We narrowed to 7,534 results for: ski

1. pCS2_IRES2_CyOFP1_KCNQ2 var4

   Plasmid

   #173155

   Purpose

   For mammalian expression of KCNQ2 and creating variants

   Depositor

   Al George

   Article

   Simkin et al Elife. 2021 Feb 5;10. pii: 64434. doi: 1

   Insert

   KCNQ2 (KCNQ2 Human)
   

   Tags

   IRES2-CyOFP1

   Expression

   Mammalian

   Promoter

   Simian CMV IE94

   Available Since

   Sept. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. HiBiT-FZD2

   Plasmid

   #195840

   Purpose

   Expresses human FZD2 with an N-terminal HiBiT

   Depositor

   Gunnar Schulte

   Article

   Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

   Insert

   FZD2 (FZD2 Human)
   

   Tags

   HiBiT

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. HiBiT-FZD4

   Plasmid

   #195842

   Purpose

   Expresses human FZD4 with an N-terminal HiBiT

   Depositor

   Gunnar Schulte

   Article

   Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

   Insert

   FZD4 (FZD4 Human)
   

   Tags

   HiBiT

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1/NDUFS3/myc-His

   Plasmid

   #127487

   Purpose

   Expresses myc-tagged NDUFS3 in mammalian cells

   Depositor

   Shashi Jain, Dieter Wolf

   Article

   Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

   Insert

   NDUFS3 (NDUFS3 Human)
   

   Tags

   myc-His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV2-CAG-mGreenLantern-KASH

   Plasmid

   #191094

   Purpose

   To express a bright monomeric green FP to label the eucaryotic nuclear envelope. To be used in tissue clearing methods and other fluorescent microscopy methods

   Depositor

   Murray Blackmore, Pantelis Tsoulfas

   Article

   Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:

   Insert

   mGreenLantern-KASH
   

   Use

   AAV and Synthetic Biology

   Tags

   mGreenLantern fused to the KASH domain of Nesprin…

   Expression

   Mammalian

   Mutation

   Optimizzation was done using the Human codon usage

   Promoter

   CMV enhancer and CAG

   Available Since

   Dec. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET21a-C2m2-SNAP

   Plasmid

   #208773

   Purpose

   To express C2m2-SNAP in bacteria

   Depositor

   Urte Neniskyte

   Article

   Dirvelyte et al Cell Mol Biol Lett. 2023 Jul 27;28(1):59

   Insert

   C2m2-SNAP (Mfge8 Mouse)
   

   Tags

   SNAP-tag

   Expression

   Bacterial

   Mutation

   Mutated 24 and 45 lysine to asparagine in C2 doma…

   Promoter

   T7 promoter

   Available Since

   Oct. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. CRY-Gal∆DD (B1013)

   Plasmid

   #92035

   Purpose

   Expresses CRY2 (full-length, with endogenous NLS) fused to Gal4 (aa1-65 of Gal4BD), downstream of mCherry-IRES

   Depositor

   Chandra Tucker

   Article

   Pathak et al Nucleic Acids Res. 2017 Apr 20. doi: 10.

   Insert

   CRY2 (CRY2 Mustard Weed)
   

   Expression

   Mammalian

   Mutation

   Fusion of plant CRY2 to Gal4 binding domain (AA1-…

   Available Since

   July 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEX Src-SH2

   Plasmid

   #46510

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   Src (SRC Human)
   

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 142-252

   Promoter

   Tac

   Available Since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-iCre-2A-cherry

   Plasmid

   #182246

   Purpose

   Expresses iCre linked to mCherry. The promoter is a fragment of the hdc gene promoter which drives pan neuronal expression.

   Depositor

   William Wisden

   Article

   Miracca et al J Neurosci. 2022 May 31. pii: JNEUROSCI.

   Insert

   pan neuronal gene promoter (hdc) fragment driving iCre recombinase and 2A linked to mCherry
   

   Use

   AAV and Cre/Lox

   Expression

   Mammalian

   Promoter

   fragment of histidine decarboxylase gene promoter…

   Available Since

   March 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV.CAG.SF-iGluSnFR.A184V

    Plasmid

    #106199

    Purpose

    Medium affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

    Insert

    SF-iGluSnFR.A184V
    

    Use

    AAV

    Mutation

    GltI: A184V

    Promoter

    CAG

    Available Since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV.hSynapsin.SF-iGluSnFR.S72A

    Plasmid

    #106176

    Purpose

    Weak affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

    Has Service

    AAV1

    Insert

    SF-iGluSnFR.S72A
    

    Use

    AAV

    Mutation

    GltI: S72A

    Promoter

    hSynapsin

    Available Since

    June 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. Basigin-isoform2-CD4d3+4-bio

    Plasmid

    #73129

    Purpose

    EXPRESs plasmid for human erythrocyte surface proteins encoding Basigin-isoform2 with rat CD4d3+4-bio

    Depositor

    Gavin Wright

    Article

    Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

    Insert

    Basigin isoform 2 (BSG Human)
    

    Tags

    biotinylation peptide and ratCD4d3+4

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pmCTIP2-N174

    Plasmid

    #60858

    Purpose

    Expresses CTIP2 under the EF1a promoter

    Depositor

    Andrew Yoo

    Article

    Victor et al Neuron. 2014 Oct 22;84(2):311-23. doi: 1

    Insert

    CTIP2 isoform b (Bcl11b Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Dec. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. p3HAT1Luc1

    Plasmid

    #236275

    Purpose

    Mammalian expression of human angiotensin II type 1 receptor; N-terminal HA tag, C-terminal Rluc8

    Depositor

    Vsevolod Gurevich

    Article

    Gimenez et al J Biol Chem. 2012 Aug 24;287(35):29495-5

    Insert

    angiotensin II type 1 receptor (AGTR1 Human)
    

    Tags

    3xHA and Rluc8

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. lentiCRISPR v2-sgBAX-1

    Plasmid

    #210013

    Purpose

    knock out BAX in mammalian cells

    Depositor

    Boyi Gan

    Article

    Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

    Insert

    Apoptosis regulator BAX (BAX Human)
    

    Use

    CRISPR

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. PB-PE

    Plasmid

    #162796

    Purpose

    All-in-one prime editor piggyBac transposon

    Depositor

    Tobias Cantz

    Article

    Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1

    Insert

    SpCas9_ H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
    

    Use

    CRISPR and Synthetic Biology; Piggybac transposon

    Tags

    SV40 NLS

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. YA1529: pAAV_hSyn-QuasAr3-P2A-CheRiff

    Plasmid

    #107700

    Purpose

    in vivo voltage imaging

    Depositor

    Adam Cohen

    Article

    Adam et al Nature. 2019 May 1. pii: 10.1038/s41586-

    Insert

    QuasAr3-P2A-CheRiff
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    human synapsin I

    Available Since

    May 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. CIB-VP64 (B1016)

    Plasmid

    #92037

    Purpose

    Expresses CIB1 (Full-length, with endogenous NLS) fused to VP64 activation domain (4xVP16 inserts)

    Depositor

    Chandra Tucker

    Article

    Pathak et al Nucleic Acids Res. 2017 Apr 20. doi: 10.

    Insert

    CIB1-VP64 (CIB1 Mustard Weed)
    

    Tags

    Fusion of plant CIB1 with VP64 activation domain.…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pC129: pAAV.CMV-CasRx mCh

    Plasmid

    #203442

    Purpose

    Plasmid expressing active RfxCas13d with mCherry reporter for investigating CasRx activity

    Depositor

    Guei-Sheung Liu

    Article

    Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

    Insert

    RfxCas13d-T2A-mCherry
    

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLV-CAG-F14F15S-Switch

    Plasmid

    #99733

    Purpose

    Flp-based FLEX reporter (from RFP to GFP)

    Depositor

    Ian Wickersham

    Article

    Chatterjee et al Nat Neurosci. 2018 Apr;21(4):638-646. do

    Insert

    TagRFP and EGFP
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Jan. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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