We narrowed to 7,454 results for: ski

1. pAAV.hSynap-FLEX.SF-iGluSnFR.S72A

   Plasmid

   #106182

   Purpose

   Weak affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

   Depositor

   Loren Looger

   Article

   Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

   Insert

   SF-iGluSnFR.S72A
   

   Use

   AAV

   Tags

   Expression

   Mutation

   GltI: S72A

   Promoter

   hSynapsin

   Available since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. YA1531: pAAV_CAG-FLEX-QuasAr3

   Plasmid

   #107701

   Purpose

   in vivo voltage imaging

   Depositor

   Adam Cohen

   Article

   Adam et al Nature. 2019 May 1. pii: 10.1038/s41586-

   Insert

   QuasAr3
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-TRE3_ mTFP1- Vamp2

   Plasmid

   #201214

   Purpose

   AAV expression of TRE(TRE3G)-driven, Vamp2-conjugated mTFP1 expression for fluorescent labling of axon terminals

   Depositor

   Tetsuo Yamamori

   Article

   Watakabe et al Neuron. 2023 May 9:S0896-6273(23)00338-0

   Insert

   mTFP1 (from addgene #54613)
   

   Use

   AAV

   Tags

   mouse Vamp2

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   TRE3G

   Available since

   July 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. CLXSN 5myc-CDC6-mut

   Plasmid

   #109333

   Purpose

   express tagged Cdc6 mutant

   Depositor

   Jean Cook

   Article

   Matson et al Elife. 2017 Nov 17;6. doi: 10.7554/eLife

   Insert

   CDC6 (CDC6 Human)
   

   Use

   Retroviral

   Tags

   5x Myc

   Expression

   Mammalian

   Mutation

   R56A, L59A, K81A, E82A, N83A

   Promoter

   Available since

   July 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. bio-CD4d3+4-Kell blood group

   Plasmid

   #73089

   Purpose

   EXPRESs plasmid for human erythrocyte surface proteins encoding Kell blood group with bio-rat CD4d3+4

   Depositor

   Gavin Wright

   Article

   Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

   Insert

   Kell blood group (KEL Human)
   

   Use

   Tags

   biotinylation peptide and ratCD4d3+4

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCC_02 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-Cas9NG-NLS-2A-Puro-WPRE

   Plasmid

   #139087

   Purpose

   Expresses human codon-optimized Cas9-NG nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.

   Depositor

   Neville Sanjana

   Article

   Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

   Insert

   SpCas9-NG
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   L1111R, D1135V,G1218R, E1219F, A1322R, R1335V, T1…

   Promoter

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. LENTICRISPR-HK2_sgRNA1

   Plasmid

   #201596

   Purpose

   CRISPR/Cas9-mediated gene knock-out

   Depositor

   Alexis Jourdain, Vamsi Mootha

   Article

   Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

   Insert

   HK2 (HK2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. HiBiT-FZD9

   Plasmid

   #195847

   Purpose

   Expresses human FZD9 with an N-terminal HiBiT; contains P199R; I448V; S524N; R556H

   Depositor

   Gunnar Schulte

   Article

   Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

   Insert

   FZD9 (FZD9 Human)
   

   Use

   Tags

   HiBiT

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. Duffy-CD4d3+4-bio

   Plasmid

   #73122

   Purpose

   EXPRESs plasmid for human erythrocyte surface proteins encoding Duffy with rat CD4d3+4-bio

   Depositor

   Gavin Wright

   Article

   Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

   Insert

   Duffy (ACKR1 Human)
   

   Use

   Tags

   biotinylation peptide and ratCD4d3+4

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBabe_puro_DEST_Flag_SOX2

    Plasmid

    #45270

    Depositor

    William Hahn

    Article

    Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

    Insert

    MCOPS3 (SOX2 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2013

    Availability

    Academic Institutions and Nonprofits only

11. KAT6B-Tol2

    Plasmid

    #113384

    Purpose

    evaluate FOXA1 DIV motif enhancer activity near 50 kb of KAT6B gene

    Depositor

    Ralf Jauch

    Article

    Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

    Insert

    KAT6B-enhancer (KAT6B Human)
    

    Use

    Tol2 reporter

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

12. pD2sE_SC10

    Plasmid

    #219716

    Purpose

    DV2 stable E dimer with fusion loop mutation

    Depositor

    Brian Kuhlman

    Article

    Kudlacek et al Sci Adv. 2021 Oct 15;7(42):eabg4084. doi

    Insert

    DV2 sE SC10
    

    Use

    Tags

    8xHis and human serum albumin (HSA)

    Expression

    Mammalian

    Mutation

    G106D, A259W, T262R, F279W, T280P

    Promoter

    Available since

    Aug. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV_hsyn_NES-his-CaMPARI2-H396K-WPRE-SV40

    Plasmid

    #101062

    Purpose

    AAV vector expressing CaMPARI2_H396K (Kd = 360nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons

    Depositor

    Eric Schreiter

    Article

    Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

    Insert

    CaMPARI2-H396K
    

    Use

    AAV

    Tags

    FLAG-HA-myc and NES_his

    Expression

    Mutation

    Promoter

    hsyn (synapsin-1)

    Available since

    Oct. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRB112

    Plasmid

    #180173

    Purpose

    pAAV construct GluN1WT (shRNA resistant)

    Depositor

    Richard Bergeron

    Article

    Cappelli et al iScience. 2021 Dec 3;25(1):103539. doi:

    Insert

    GluN1 (Grin1 Rat)
    

    Use

    AAV

    Tags

    Phluorin

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. bio-CD4d3+4-TMEM24

    Plasmid

    #73092

    Purpose

    EXPRESs plasmid for human erythrocyte surface proteins encoding TMEM24 with bio-rat CD4d3+4

    Depositor

    Gavin Wright

    Article

    Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

    Insert

    TMEM24 (C2CD2L Human)
    

    Use

    Tags

    biotinylation peptide and ratCD4d3+4

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. KAT6B-luciferase

    Plasmid

    #113353

    Purpose

    evaluate FOXA1 DIV motif enhancer activity near 50 kb of KAT6B gene

    Depositor

    Ralf Jauch

    Article

    Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

    Insert

    KAT6B-enhancer (KAT6B Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. GlycophorinC-CD4d3+4-bio

    Plasmid

    #73096

    Purpose

    EXPRESs plasmid for human erythrocyte surface proteins encoding GlycophorinC with rat CD4d3+4-bio

    Depositor

    Gavin Wright

    Article

    Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

    Insert

    Glycophorin C (GYPC Human)
    

    Use

    Tags

    biotinylation peptide and ratCD4d3+4

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCIBN-EYFP-hTRF2

    Plasmid

    #103804

    Purpose

    BLInCR 'Localizer' construct that marks the telomeres and is targeted by a PHR-tagged effector upon illumination with blue light

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    CIBN-EYFP-hTRF2 (CIB1, TERF2 Mustard Weed, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    hTRF2: deletion of amino acids 1-42 and 476 compa…

    Promoter

    CMV

    Available since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. MCP-3x9aaTAD

    Plasmid

    #210707

    Purpose

    This Plasmid express EF1a promoter driven MCP-3x 9aa transactivation module

    Depositor

    Isaac Hilton

    Article

    Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

    Insert

    MCP fused to 3x 9aa transactivation domain derived from MRTF-B and MYOCD1
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    N55K in MCP

    Promoter

    EF1a

    Available since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET30a-His6-ABP-LOXCATmut

    Plasmid

    #139972

    Purpose

    A control for His6-ABP-LOXCAT where LOX is catalytically dead (mutations H265A and R268A)

    Depositor

    Vamsi Mootha

    Article

    Patgiri et al Nat Biotechnol. 2020 Jan 13. pii: 10.103

    Insert

    A fusion of lactate oxidase from A. viridans and catalase from E. coli
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Mutation in H265A and R268A in LOX (used original…

    Promoter

    T7

    Available since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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