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We narrowed to 10,084 results for: Coli

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Showing: 5061 - 5080 of 10084 results

  10. GFP-itis pBE-nt

    Plasmid
    #195343
    Purpose
    Base editor plasmid expressing ABE8e-NG-Cas9n under Theophylline Riboswitch and constituitively expressed nonsense non-targeting gRNA
    Depositor
    Alexis Komor
    Article
    Vasquez et al CRISPR J. 2023 Apr 20. doi: 10.1089/cris
    Inserts
    ecTadA(8e)-SpCas9-NG
    gRNA gtgcacgacgccgtatgcga
    Use
    CRISPR
    Mutation
    SpCas9n-NG: D10A, L1111R, D1135V, G1218R, E1219F,…
    Promoter
    Lac and ProC
    Available Since
    Jan. 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    195343-plasmid-map-sequence-id-383225

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  16. pKD072

    Plasmid
    #136472
    Purpose
    SSB-C-term-GFP fusion expression plasmid
    Depositor
    James Keck
    Article
    Dubiel et al Nucleic Acids Res. 2020 Jun 19;48(11):60
    Insert
    SSB-C-term-GFP (ssb E. coli)
    Tags
    superfolder GFP
    Expression
    Bacterial
    Mutation
    superfolder GFP attached to C-terminus at Phe 178
    Promoter
    T7 promoter
    Available Since
    April 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    136472-plasmid-map-sequence-id-267962

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  17. pCCM

    Plasmid
    #162708
    Purpose
    Second CCM operon cloned from H. neapolitanus; pFA expressing the remaining 11 genes in the H. neapolitanus CCM cluster
    Depositor
    David Savage
    Article
    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1
    Insert
    Second CCM operon cloned from H. neapolitanus
    Expression
    Bacterial
    Promoter
    PLtet0-1 promoter
    Available Since
    Feb. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    162708-plasmid-map-sequence-id-324195

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  18. pH6-TEV-spTorA-GFP(C)

    Plasmid
    #169041
    Purpose
    GFP preceeded by the signal peptide of TorA (spTorA) with an N-terminal 6xHis tag and TEV cleavage site in pBAD24.
    Depositor
    Siegfried Musser
    Article
    Bageshwar et al PLoS One. 2021 Sep 9;16(9):e0256715. doi
    Insert
    H6-spTorA-GFP
    Tags
    6xHis, TEV cleavage site, and spTorA (signal pept…
    Expression
    Bacterial
    Mutation
    The mut3 GFP variant with 5 additional mutations …
    Promoter
    araBAD
    Available Since
    Oct. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    169041-plasmid-map-sequence-id-346121

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  19. pNAS1B split mCherry N-ME(pS)VD/TRAP(RK)-C

    Plasmid
    #61966
    Purpose
    Positive control duet plasmid for SepOTS-dependent split mCherry assembly assay (i.e., N-mCherry is fused to the peptide ME(pS)VD (pS = phosphoserine); C-mCherry is fused to TRAP(RK))
    Depositor
    Lynne Regan
    Article
    Sawyer et al ACS Chem Biol. 2014 Nov 21;9(11):2502-7.
    Inserts
    N-mCherry-ME(pS)VD
    TRAP(RK)-C-mCherry
    Expression
    Bacterial
    Promoter
    PBAD and PLtetO
    Available Since
    Feb. 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    61966-plasmid-map-sequence-id-101265

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  20. pSCL.177

    Plasmid
    #184998
    Purpose
    Express -Eco1 EMX1 editing ncRNA and gRNA
    Depositor
    Seth Shipman
    Article
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1: EMX1 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
    Tags
    SV40NLS
    Expression
    Mammalian
    Mutation
    EMX1 donor, a1/a2 length extended for 27 bp
    Promoter
    H1
    Available Since
    Nov. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    184998-plasmid-map-sequence-id-376623

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Showing: 5061 - 5080 of 10084 results