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pQC-SEAP #M004#725

Plasmid

#42125

Depositor

Ayano Satoh

Article

Koreishi et al PLoS One. 2013;8(1):e54382. doi: 10.1371

Insert

alkaline phosphatase, placental (SEAP) (ALPP Human)

Use

Retroviral

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 20, 2013

Availability

Academic Institutions and Nonprofits only
pZR069_CROPseq-sgPTPRC-CRISPRi

Plasmid

#180271

Purpose

Lentiviral CROPseq vector expressing PTPRC (CD45) targeting sgRNA for CRISPRi

Depositor

Alexander Marson

Article

Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

Insert

PTPRC (CD45) sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pADV-Sec61b-TurboID

Plasmid

#179143

Purpose

Recombinant adenoviral plasmid for Sec61b-TurboID expression

Depositor

Jae Myoung Suh

Article

Kim et al Nat Commun. 2021 Sep 1;12(1):5204. doi:

Insert

Sec61b-V5-TurboID

Use

Adenoviral

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pDAI-SceI-SacB

Plasmid

#113635

Purpose

Broad host range replicative plasmid expressing the I-SceI homing endonuclease and the counterselectable marker SacB

Depositor

Miguel Valvano

Article

Aubert et al Methods Mol Biol. 2014;1197:311-27. doi:

Insert

sacB

Expression

Bacterial

Mutation

Please see Depositor Comments

Promoter

sacB promoter

Available Since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pTRIPZ-EGFP:LIMD1

Plasmid

#108230

Purpose

Lentiviral vector for Tet-inducible LIMD1 expression

Depositor

Kenneth Irvine

Article

Ibar et al J Cell Sci. 2018 Feb 13. pii: jcs.214700

Insert

LIMD1 (LIMD1 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

Tet/ON Minimal CMV promoter

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-neo-Strep_Flag_Cterm

Plasmid

#102645

Purpose

Epitope tag c-terminus with SBP-Flag moiety

Depositor

Michael E. Wright

Article

Hsiao et al BMC Cancer. 2015 Mar 31;15:204. doi: 10.

Type

Empty backbone

Tags

SBP-Flag, MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREPG…

Expression

Mammalian

Available Since

Oct. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pDD121

Plasmid

#91833

Purpose

Cas9 expression in C. elegans

Depositor

Bob Goldstein

Article

Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

Insert

Cas9

Use

CRISPR

Expression

Worm

Mutation

Codon optimized and with synthetic introns for C.…

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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hTRIM65_EGFP_N1

Plasmid

#54320

Purpose

Mammalian expression vector for human TRIM65 fusion protein with c-terminal EGFP.

Depositor

Martin Dorf

Article

Li et al Proc Natl Acad Sci U S A. 2014 May 13;11

Insert

TRIM65 (TRIM65 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

July 1, 2014

Availability

Academic Institutions and Nonprofits only

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pSMP-SETDB1_1

Plasmid

#36347

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

SETDB1 (SETDB1 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
FLAG-ARP8

Plasmid

#15361

Depositor

Joan Conaway, Ronald Conaway

Article

Jin et al J Biol Chem. 2005 Dec 16. 280(50):41207-

Insert

actin-related protein 8 (ACTR8 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Aug. 17, 2007

Availability

Academic Institutions and Nonprofits only

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SBFP2-anti-AlfaTag nanobody

Plasmid

#171826

Purpose

Bacterial expression vector for production of fluorescent anti-AlfaTag nanobody

Depositor

Ikuo Wada

Article

Matsumoto et al J Biol Chem. 2021 Jul;297(1):100851. doi

Insert

SBFP2-anti-AlfaTag nanobody

Tags

6xHis and SEP tag (6xD)

Expression

Bacterial

Available Since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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pTRIPZ-TIR1-HA

Plasmid

#172610

Purpose

Lentiviral vector for the Tet-inducible expression of HA-tagged TIR1 and a constitutive puromycin resistance marker in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

TIR1

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

Tet/ON Minimal CMV promoter

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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MS03

Plasmid

#164526

Purpose

Expresses Sonic Hedgehog

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Sonic Hedgehog (SHH Human)

Use

Lentiviral

Mutation

WT

Promoter

CMV

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-CMVSP6-nEGFP-SV40-PURO

Plasmid

#138364

Purpose

Lentiviral plasmid for nuclear EGFP expression driven by CMVSP6 and puromycin resistance under SV40 promoter for selection

Depositor

Nathan Lawson

Article

Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC

Insert

nuclear EGFP

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-RAB10 T23N

Plasmid

#130885

Purpose

Expresses human RAB10 T23N mutant with N-terminal GFP tag

Depositor

Michael Bassik

Article

Jeng et al Cell Host Microbe. 2019 Oct 9;26(4):551-

Insert

RAB10 (RAB10 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

T23N

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pSMP-Bmi1_1

Plasmid

#36352

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

Bmi1 (BMI1 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only

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pSRP-mTAZ

Plasmid

#31795

Depositor

Michael Yaffe

Article

Hong et al Science. 2005 Aug 12;309(5737):1074-8.

Insert

TAZ

Use

RNAi and Retroviral

Promoter

RNA Pol-III

Available Since

Aug. 29, 2011

Availability

Academic Institutions and Nonprofits only
pLPCXU67SKTP

Plasmid

#27662

Depositor

Kathleen Collins

Article

Hogg et al RNA. 2007 Jun . 13(6):868-80.

Insert

U67SKTP (RN7SK Human)

Use

Retroviral

Expression

Mammalian

Available Since

April 20, 2011

Availability

Academic Institutions and Nonprofits only

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cmv Flag/HA 16 E7

Plasmid

#13734

Depositor

Karl Munger

Article

Baldwin et al J Virol. 2006 Jul . 80(13):6669-77.

Insert

HPV 16 E7 (E7 Human)

Tags

Flag and HA

Expression

Mammalian

Available Since

March 12, 2007

Availability

Academic Institutions and Nonprofits only
pDONR223 SARS-CoV-2 ORF3B_nostop

Plasmid

#149320

Purpose

Gateway-compatible Entry vector, with insert of ORF3B gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

ORF3B

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available Since

May 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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