We narrowed to 7,101 results for: tev

1. pY71-tet.I.1

   Plasmid

   #133523

   Purpose

   sfGFP driven by a T7 promoter with the tetO sequence 4 bp downstream from the promoter start site

   Depositor

   Richard Murray

   Article

   McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

   Insert

   sfGFP
   

   Use

   Tags

   Strep tag and TEV site

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available since

   Dec. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJSC176 - Bacterial expression plasmid for SpCas9 + Q926A variant

   Plasmid

   #101206

   Purpose

   Bacterial expression plasmid for SpCas9 + Q926A variant

   Depositor

   Jennifer Doudna, Keith Joung

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant Q926A
   

   Use

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   Q926A

   Promoter

   T7

   Available since

   Nov. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET151-Vps4(101-437)-Hcp1 K321D

   Plasmid

   #89288

   Purpose

   Bacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1

   Depositor

   Chris Hill

   Article

   Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

   Insert

   Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
   

   Use

   Tags

   6xHis-V5-TEV and Hcp1

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-100, mutated Lys-321 to Asp

   Promoter

   T7

   Available since

   April 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET151-Vps4(101-437)-Hcp1 R325D

   Plasmid

   #89289

   Purpose

   Bacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1

   Depositor

   Chris Hill

   Article

   Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

   Insert

   Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
   

   Use

   Tags

   6xHis-V5-TEV and Hcp1

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-100, mutated Arg-325 to Asp

   Promoter

   T7

   Available since

   April 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET151-Vps4(101-437)-Hcp1 E322A

   Plasmid

   #87748

   Purpose

   Bacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1

   Depositor

   Chris Hill

   Article

   Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

   Insert

   Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
   

   Use

   Tags

   6xHis-V5-TEV and Hcp1

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-100, mutated Glu-322 to Ala

   Promoter

   T7

   Available since

   April 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET151-Vps4(101-437)-Hcp1 R325A

   Plasmid

   #87749

   Purpose

   Bacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1

   Depositor

   Chris Hill

   Article

   Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

   Insert

   Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
   

   Use

   Tags

   6xHis-V5-TEV and Hcp1

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-100, mutated Arg-325 to Ala

   Promoter

   T7

   Available since

   April 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET151-Vps4(101-437)-Hcp1 K321A

   Plasmid

   #87747

   Purpose

   Bacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1

   Depositor

   Chris Hill

   Article

   Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

   Insert

   Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
   

   Use

   Tags

   6xHis-V5-TEV and Hcp1

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-100, mutated Lys-321 to Ala

   Promoter

   T7

   Available since

   April 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET151-hUCH37 (ISF1)(M148D/F149D)

   Plasmid

   #71388

   Purpose

   Expression of hUCH37 (M148D/F149D) in E.coli

   Depositor

   Chris Hill

   Article

   VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

   Insert

   hUCH37 (ISF1) M148D/F149D (UCHL5 Human)
   

   Use

   Tags

   HIS-TEV sequence

   Expression

   Bacterial

   Mutation

   changed methionine 148 to aspartate, phenylalanin…

   Promoter

   T7

   Available since

   Dec. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pEF5B-FRT-GFP-αTAT1

   Plasmid

   #27099

   Depositor

   Maxence Nachury

   Article

   Shida et al Proc Natl Acad Sci U S A. 2010 Dec 14;10

   Insert

   α-Tubulin K40 acetyltransferase1 (ATAT1 Human)
   

   Use

   Tags

   GFP, S-tag, and TEV site

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 5, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGTM_YR375_SUMO_Protease_001

    Plasmid

    #190063

    Purpose

    Expresses the yeast Ulp1 Sumo protease in active form as a TEV protease-cleavable MBP fusion

    Depositor

    Gaetano Montelione

    Article

    Mazzei et al STAR Protoc. 2023 May 5;4(2):102326. doi

    Insert

    ULP1_YEAST (ULP1 Budding Yeast)
    

    Use

    Tags

    8X His-MBP (Maltose Binding Protein)

    Expression

    Bacterial

    Mutation

    Construct contains residues 347 – 621

    Promoter

    T7 lac

    Available since

    Jan. 30, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pGEX-DAXX-His

    Plasmid

    #175782

    Purpose

    DAXX protein for bacteria expression system

    Depositor

    Xiaolu Yang

    Article

    Huang et al Nature. 2021 Aug 18. pii: 10.1038/s41586

    Insert

    death domain associated protein (DAXX Human)
    

    Use

    Tags

    GST and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFastBac Dual α1B/β2B Tubulin

    Plasmid

    #170553

    Purpose

    Encodes codon-optimized human α1B and β2B tubulin for expression in and purification from insect cells.

    Depositor

    Tarun Kapoor

    Article

    Ti et al Dev Cell. 2018 Oct 22;47(2):175-190.e5.

    Inserts

    TUBA1B (TUBA1B Human)
    TUBB2B (TUBB2B Human)
    

    Use

    Tags

    AP linker, GS linker (GGSGG), His10 tag, L21 enha…

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pFastBac Dual α1B/β3 Tubulin

    Plasmid

    #170552

    Purpose

    Encodes codon-optimized human α1B and β3 tubulin for expression in and purification from insect cells.

    Depositor

    Tarun Kapoor

    Article

    Ti et al Dev Cell. 2018 Oct 22;47(2):175-190.e5.

    Inserts

    TUBA1B (TUBA1B Human)
    TUBB3 (TUBB3 Human)
    

    Use

    Tags

    AP linker, GS linker (GGSGG), His10 tag, L21 enha…

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTF023_His_hsDDA1

    Plasmid

    #174480

    Purpose

    Expresses DDA1 in insect cells

    Depositor

    Eric Fischer

    Article

    Faust et al Nat Chem Biol. 2020 Jan;16(1):7-14. doi:

    Insert

    DDA1 (DDA1 Human)
    

    Use

    Tags

    His-TEV

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. IMPT-10316:GABR1

    Plasmid

    #157850

    Purpose

    HA-TEV-VFT-TMD-CC-PreX-eGFP in pFastBac1 for Sf9 expression

    Depositor

    Raymond Stevens, Vadim Cherezov

    Article

    Shaye et al Nature. 2020 Jun 17. pii: 10.1038/s41586

    Insert

    GABR1 (GABBR1 Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    Aug. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET41-MBP-t-A3A-t-His

    Plasmid

    #109231

    Purpose

    Bacterial expression of A3A with N-terminal MBP an C-terminal His tag, both cleavable with TEV protease

    Depositor

    Rahul Kohli

    Article

    Schutsky et al Nat Biotechnol. 2018 Oct 8. pii: nbt.420

    Insert

    human APOBEC3A (APOBEC3A Human, Optimized for E. coli expression)
    

    Use

    Tags

    His and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFastBac-GST-DAXX-His

    Plasmid

    #175781

    Purpose

    DAXX protein for baculovirus expression system

    Depositor

    Xiaolu Yang

    Article

    Huang et al Nature. 2021 Aug 18. pii: 10.1038/s41586

    Insert

    death domain associated protein (DAXX Human)
    

    Use

    Tags

    GST and TEV

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGEX4T3-GST-NQO1

    Plasmid

    #177140

    Purpose

    Bacterial vector for expression of an N-terminal GST fusion of NQO1 with a TEV protease site located between the GST tag and NQO1

    Depositor

    Robert Sobol

    Article

    Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

    Insert

    NQO1 (NQO1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Tac

    Available since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHY002_Strep_Avi_hsDCAF15

    Plasmid

    #174479

    Purpose

    Expresses DCAF15 in insect cells

    Depositor

    Eric Fischer

    Article

    Faust et al Nat Chem Biol. 2020 Jan;16(1):7-14. doi:

    Insert

    DCAF 15 (DCAF15 Human)
    

    Use

    Tags

    StrepII-Avi-TEV

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJSC173 - Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9) variant

    Plasmid

    #101218

    Purpose

    Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9) variant

    Depositor

    Jennifer Doudna, Keith Joung

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant N692A/M694A/Q695A/H698A
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    N692A, M694A, Q695A and H698A

    Promoter

    T7

    Available since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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