Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 12,221 results for: LEA

Clear keywords
Showing: 5081 - 5100 of 12221 results

  1. pFUSEss-CHIg-mM-Asn209Asp_M18

    Plasmid
    #91781
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Asp (EU numbering).
    Depositor
    Joanna Bereta
    Publication
    Klaus et al Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1
    Insert
    immunoglobulin heavy constant mu (Ighm Mouse)
    Expression
    Mammalian
    Mutation
    Asn209 changed to Asp (EU numbering)
    Promoter
    hEF1-HTLV prom
    Available since
    June 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    91781-plasmid-map-sequence-id-181014

    Enlarge

  2. pEGFPN1-N-gamma-tubulin (334-449)

    Plasmid
    #87859
    Purpose
    Fluorescent fragment of gamma-tubulin containing gamma-tubulin DNA binding domain and NLS
    Depositor
    Maria Alvarado-Kristensson
    Publication
    Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    gamma-tubulin 1 (TUBG1 Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    fragment from aa 334 to 449
    Available since
    May 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    87859-plasmid-map-sequence-id-178206

    Enlarge

  3. pYFP-ErbB2ΔC990

    Plasmid
    #66946
    Purpose
    Mammalian expression of rat ErbB2 ΔC990 tagged with YFP
    Depositor
    Mads Lerdrup, Bo van_Deurs
    Publication
    Lerdrup et al Mol Biol Cell. 2007 Sep;18(9):3656-66. E
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ErbB2 (Erbb2 Rat)
    Tags
    6xHis, V5 Tag, and YFP
    Expression
    Mammalian
    Mutation
    Deletion of C990. (see depositor comment below on…
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  4. 3xFN-Tel-TAL/pMXs-puro

    Plasmid
    #63590
    Purpose
    Expresses a 3xFLAG-tagged TAL protein recognizing a telomere repeat. A retroviral expression vector with the puromycin resistance gene.
    Depositor
    Hodaka Fujii
    Publication
    Fujita et al Sci Rep. 2013 Nov 8;3:3171. doi: 10.1038
    Insert
    3xFVN-Tel-TAL
    Use
    Retroviral and TALEN
    Tags
    3xFLAG-tag, V5-tag, and nuclear localization sign…
    Expression
    Mammalian
    Promoter
    LTR
    Available since
    April 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    63590-plasmid-map-sequence-id-108379

    Enlarge

  11. pEJS2708-CMV-Nme2Smu-ABE8e-i1-L10-E932R

    Plasmid
    #228729
    Purpose
    Expresses domain inlaid Nme2Smu nCas9 (D16A)-i1-ABE8e base editor with linker-10 and E932R mutation in mammalian cells
    Depositor
    Erik Sontheimer
    Publication
    Bamidele et al bioRxiv [Preprint]. 2024 Nov 7:2024.10.3
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Domain inlaid Nme2Smu nCas9 (D16A)-i1-L10-E932R
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Nme2-Cas9, Smu PID, D16A E932R
    Promoter
    CMV
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    228729-plasmid-map-sequence-id-454007

    Enlarge

  12. Polymerase-unfused click editor - pCMV-T7-PCV2-nCas9 (LM2702)

    Plasmid
    #208961
    Purpose
    Polymerase-unfused click editor comprised of PCV2 fused to nCas9, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Publication
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PCV2-XTEN-nSpCas9-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A)
    Promoter
    CMV and T7
    Available since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208961-plasmid-map-sequence-id-415791

    Enlarge

  13. pcDNA3-AktAR-Kras

    Plasmid
    #123357
    Purpose
    FRET-based biosensor for monitoring Akt activity in non-raft plasma membrane regions.
    Depositor
    Jin Zhang
    Publication
    Gao et al Mol Biol Cell. 2008 Oct;19(10):4366-73.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AktAR-Kras
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Nov. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    123357-plasmid-map-sequence-id-307467

    Enlarge

  17. pcDNA3-Venus-cpVenus-FLARE-ICUE

    Plasmid
    #123355
    Purpose
    Yellow-fluorescent homoFRET/anisotropy-based cAMP indicator. Contains monomeric Venus/cpVenus-E172 homoFRET pair.
    Depositor
    Jin Zhang
    Publication
    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Venus-cpVenus-FLARE-ICUE
    Tags
    6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    123355-plasmid-map-sequence-id-307489

    Enlarge

  18. pcDNA3-mCherry-mCherry-FLARE-AKAR

    Plasmid
    #123333
    Purpose
    Red-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity.
    Depositor
    Jin Zhang
    Publication
    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCherry-mCherry-FLARE-AKAR
    Tags
    6xHIS, T7 tag (gene 10 leader), Xpress (TM) tag, …
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    123333-plasmid-map-sequence-id-306922

    Enlarge

  19. pGBW-m4046919

    Plasmid
    #145615
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 nsp7
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 nsp7 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;StrepII
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145615-plasmid-map-sequence-id-300779

    Enlarge

  20. pGBW-m4046899

    Plasmid
    #145608
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 ORF6 protein
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 ORF6 protein (ORF6 Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;6xHIS
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 28, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145608-plasmid-map-sequence-id-292566

    Enlarge

Showing: 5081 - 5100 of 12221 results