We narrowed to 15,565 results for: LEA

1. TR- ATG-HP-GFP

   Plasmid

   #80592

   Purpose

   Expresses GFP with an Csy4 target hairpin inserted immediately following the start codon for Csy4-mediated regulation. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.

   Depositor

   Aravind Asokan

   Article

   Borchardt et al RNA. 2015 Nov;21(11):1921-30. doi: 10.12

   Insert

   GFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Inserted 30 nt hairpin which is targeted by Csy4 …

   Promoter

   CBA

   Available since

   Oct. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJL2

   Plasmid

   #184130

   Purpose

   For rescuing null mrp-1 mutation and labelling mrp-1 protein. Translational fusion construct. pPD95.75_Pmrp-1::mrp-1(isoform C cDNA)::gfp.

   Depositor

   Alejandro Aballay

   Article

   Lalsiamthara et al Commun Biol. 2022 May 5;5(1):422. doi: 1

   Insert

   MRP-1 (mrp-1 Nematode)
   

   Use

   Tags

   GFP

   Expression

   Worm

   Mutation

   Promoter

   Pmrp-1

   Available since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJL1

   Plasmid

   #184129

   Purpose

   For rescuing null mrp-1 mutation. pPD95.77_Pmrp-1::mrp-1(isoform C cDNA)::SL2::gfp. SL2 prevents fusion of MRP-1 and GFP.

   Depositor

   Alejandro Aballay

   Article

   Lalsiamthara et al Commun Biol. 2022 May 5;5(1):422. doi: 1

   Insert

   MRP-1 (mrp-1 Nematode)
   

   Use

   Tags

   GFP

   Expression

   Worm

   Mutation

   Promoter

   Pmrp-1 (3kb)

   Available since

   June 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMKO.1-shPrc1

   Plasmid

   #160959

   Purpose

   Prc1 shRNA in pMKO.1 retroviral vector

   Depositor

   Matthew Steinhauser

   Article

   Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

   Insert

   Prc1 shRNA (Prc1 Mouse)
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Dec. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. PcDNA-DEST47-CypB-Del3 C-term GFP

   Plasmid

   #36136

   Depositor

   Patrick Van Oostveldt

   Article

   Dieriks et al Int J Mol Med. 2012 Mar 13. doi: 10.3892

   Insert

   cyclophilin B (PPIB Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   contains amino acids 39-216 of cyclophilin B; G63…

   Promoter

   CMV

   Available since

   Nov. 1, 2012

   Availability

   Academic Institutions and Nonprofits only

6. pAAV-CaMKIIa-ChRger1-TS-EYFP

   Plasmid

   #127244

   Purpose

   High photocurrent, low-light sensitive channelrhodopsin (ChRger1) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CamKIIa promoter.

   Depositor

   Viviana Gradinaru

   Article

   Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

   Insert

   ChRger1-TS-EYFP
   

   Use

   AAV

   Tags

   EYFP and SpyTag

   Expression

   Mammalian

   Mutation

   Promoter

   CamKIIa

   Available since

   Sept. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pROSA26-G19

   Plasmid

   #21711

   Depositor

   Philippe Soriano

   Article

   Zambrowicz et al Proc Natl Acad Sci U S A. 1997 Apr 15. 9

   Insert

   ROSA26 G19 (Gt(ROSA)26Sor Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 28, 2009

   Availability

   Academic Institutions and Nonprofits only

8. pLSU4mg10

   Plasmid

   #126079

   Purpose

   Plant transformation vector for stable expression of mitochondria targeted GFP1-10 in plant cells

   Depositor

   Ralf-Bernd Klösgen

   Article

   Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

   Insert

   mitochondria Rieske iron sulphur protein (1-100 aa)
   

   Use

   Binary

   Tags

   GFP1-10

   Expression

   Plant

   Mutation

   Promoter

   'long' CaMV35S

   Available since

   Aug. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAGGS-PB1-MontiRed-LOV2dark

   Plasmid

   #178874

   Purpose

   Mammalian expression of PB1-MontiRed-LOV2dark

   Depositor

   Shinya Tsukiji

   Article

   Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

   Insert

   PB1-MontiRed-LOV2dark
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   LOV2: C450A, L514K, G528A, L531E, N538E

   Promoter

   CAG

   Available since

   Jan. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. UR1Ct (265-1040)

    Plasmid

    #115647

    Purpose

    expression of truncated Rag1 including the RING, E3 ubiquitin ligase (265-1008) and C-terminal region (384-1040)

    Depositor

    Martin Gellert

    Article

    Grundy et al Proc Natl Acad Sci U S A. 2010 Dec 28;10

    Insert

    Rag1 (265-1040) (Rag1 Mouse)
    

    Use

    Tags

    8XHis-MBP-PreScission

    Expression

    Mammalian

    Mutation

    truncated Rag1 (aa 265-1040)

    Promoter

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. CD23b in pIRES2eGFP

    Plasmid

    #19136

    Depositor

    Zena Werb

    Article

    Lemieux et al J Biol Chem. 2007 May 18. 282(20):14836-

    Insert

    CD23 beta (FCER2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

12. pET30b GluBP600n

    Plasmid

    #119835

    Purpose

    Production of recombinant GluBP600n high affinity protein in E. coli

    Depositor

    Katalin Torok

    Article

    Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

    Insert

    GluBP600n
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pProvector_AAV2_586_PLGLAR

    Plasmid

    #129024

    Purpose

    Recombinant AAV2 rep/cap plasmid modified to include an 18 amino acid peptide at cap position 586.

    Depositor

    Junghae Suh

    Article

    Tong et al J Control Release. 2019 Jun 25. pii: S01

    Insert

    Rep2/Cap2 with PLGLAR lock at 586
    

    Use

    AAV

    Tags

    Expression

    Mutation

    inserted peptide motif (PLGLARGDDDDGPLGLAR) at po…

    Promoter

    Available since

    May 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. M48 GST-TEV-Chibby-N

    Plasmid

    #17196

    Depositor

    Randall Moon

    Article

    Takemaru et al Nature. 2003 Apr 24. 422(6934):905-9.

    Insert

    Chibby (CBY1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    amino acids 1-63

    Promoter

    Available since

    Sept. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

15. pBT296_pBS-U-attP-FRT5-pSV40-Neo-pA-FRT5

    Plasmid

    #52537

    Purpose

    Expression of FRT5-flanked Neo; homologous recombination targeting vector cloning

    Depositor

    Liqun Luo

    Article

    Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

    Inserts

    PhiC31o attP (full length)
    Neo
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBT298_pBS-U-attPx3-FRT5-pSV40-Neo-pA-FRT5

    Plasmid

    #52538

    Purpose

    Expression of FRT5-flanked Neo; homologous recombination targeting vector cloning

    Depositor

    Liqun Luo

    Article

    Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

    Inserts

    PhiC31o attPx3 (70 bp each)
    Neo
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET41a iGlul

    Plasmid

    #119832

    Purpose

    Production of recombinant iGluSnFR T92A low affinity variant, a single-wavelength glutamate indicator, in E. coli

    Depositor

    Katalin Torok

    Article

    Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

    Insert

    iGlul
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    T92A

    Promoter

    T7

    Available since

    Jan. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pIS1 DICER1 short UTR

    Plasmid

    #21648

    Depositor

    David Bartel

    Article

    Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

    Insert

    Dicer 1 (DICER1 Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-hB50

    Plasmid

    #18967

    Depositor

    Mariano Viapiano

    Article

    Hu et al J Biol Chem. 2008 Sep 5;283(36):24848-59

    Insert

    Brevican N-terminus (BCAN Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Human brevican N-terminus aa 1-400

    Promoter

    Available since

    Dec. 12, 2008

    Availability

    Academic Institutions and Nonprofits only

20. pET21-pelB-LaG-2

    Plasmid

    #172739

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-2; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-2
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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