We narrowed to 13,358 results for: Mpl;

1. pEBTetD-β-tubulin_SNAP

   Plasmid

   #136832

   Purpose

   Mammalian expression of the centrosomal protein _-tubulin C-terminally fused to SNAP-tag

   Depositor

   Kai Johnsson

   Article

   Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

   Insert

   TUBB_SNAP (TUBB Human, Synthetic)
   

   Tags

   FLAG-tag / His-tag

   Expression

   Mammalian

   Promoter

   CMV-TetO2

   Available Since

   April 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHis-hPim1

   Plasmid

   #100722

   Purpose

   Expression of human Pim1 kinase domain in E.coli

   Depositor

   Byung Il Lee

   Article

   Lee et al PLoS One. 2013 Jul 31;8(7):e70358. doi:

   Insert

   Pim1 (PIM1 Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   Residues 29-313 of isoform 2

   Promoter

   T7 promotor

   Available Since

   Oct. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGAD-Atg17

   Plasmid

   #121386

   Purpose

   For Yeast-two-hybrid analysis

   Depositor

   Daniel Klionsky

   Article

   Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

   Insert

   ATG17
   

   Expression

   Yeast

   Promoter

   ADH1 promoter

   Available Since

   March 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. HS-DN-Ect2

   Plasmid

   #105956

   Purpose

   heat shock inducible transgenesis

   Depositor

   Caren Norden

   Article

   Dzafic et al Cell Rep. 2015 Oct 6;13(1):168-182. doi:

   Insert

   Ect2 (ECT2 Human)
   

   Tags

   EGFP

   Expression

   Bacterial

   Available Since

   Feb. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSLQ2804 pHR: U6-SpsgTRE3G CMV-PYL1-VPR-IRES-mCherry

   Plasmid

   #84258

   Purpose

   Expresses Sp sgTRE3G gRNA with ABA-inducible VPR and mCherry for OR gate

   Depositor

   Stanley Qi

   Article

   Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

   Inserts

   Sp sgTRE3G
   PYL1-VPR
   

   Use

   CRISPR and Lentiviral

   Tags

   IRES-mCherry and PYL1

   Expression

   Mammalian

   Promoter

   CMV and mouse U6

   Available Since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTS108 pHAGE2 CMVtetO2 MCS-3C-SUMOstar-22xGS-GFP

   Plasmid

   #199361

   Purpose

   Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally 3C-SUMOstar-GFP-tagged protein in mammalian cells.

   Depositor

   Rebecca Voorhees

   Article

   Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   3C-SUMOstar-EGFP

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTS109 pHAGE2 CMVtetO2 MCS-TEV-SUMOstar-22xGS-GFP

   Plasmid

   #199362

   Purpose

   Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally TEV-SUMOstar-GFP-tagged protein in mammalian cells.

   Depositor

   Rebecca Voorhees

   Article

   Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   TEV-SUMOstar-EGFP

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFPC1/GgVcl 1-851 A50I mutant

   Plasmid

   #46269

   Depositor

   Susan Craig

   Article

   Cohen et al J Biol Chem. 2006 Jun 9;281(23):16006-15

   Insert

   Vcl 1-851 A50I mutation (VCL Chicken)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   1-851 (Vh) head domain, A50I mutation

   Promoter

   CMV

   Available Since

   July 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pNIC-Bio2_SMAC(delta(55))_9His

   Plasmid

   #197976

   Purpose

   E. coli expression of SMAC(delta(55))-9His

   Depositor

   Eric Fischer

   Article

   Hunkeler et al Science. 2023 Mar 17;379(6637):1105-1111

   Insert

   Smac (DIABLO Human)
   

   Tags

   9His

   Expression

   Bacterial

   Mutation

   delta (1-55)

   Available Since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCFJ2300 - [1-2] ENTR - Fluor - ce-Dendra2(1 intron, syntrons(3), no_atg, no_stop)

    Plasmid

    #159869

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Fluor - ce-Dendra2(1 intron, syntrons(3), no_atg, no_stop)
    

    Expression

    Worm

    Mutation

    Not applicable

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAGGS-FLAG-BMAL1-S513A

    Plasmid

    #186831

    Purpose

    Expresses FLAG-BMAL1-S513A in mammalian cells

    Depositor

    Hung-Chun Chang

    Article

    Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

    Insert

    bmal1 (Bmal1 Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed Serine 513 to Alanine

    Available Since

    April 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAGGS-FLAG-BMAL1-S515A

    Plasmid

    #186832

    Purpose

    Expresses FLAG-BMAL1-S515A in mammalian cells

    Depositor

    Hung-Chun Chang

    Article

    Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

    Insert

    bmal1 (Bmal1 Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed Serine 515 to Alanine

    Available Since

    April 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAGGS-FLAG-BMAL1-S516A

    Plasmid

    #186833

    Purpose

    Expresses FLAG-BMAL1-S516A in mammalian cells

    Depositor

    Hung-Chun Chang

    Article

    Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

    Insert

    bmal1 (Bmal1 Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed Serine 516 to Alanine

    Available Since

    April 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAGGS-FLAG-BMAL1-S519A

    Plasmid

    #186834

    Purpose

    Expresses FLAG-BMAL1-S519A in mammalian cells

    Depositor

    Hung-Chun Chang

    Article

    Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

    Insert

    bmal1 (Bmal1 Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed Serine 519 to Alanine

    Available Since

    April 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pmcsg7-mCRT(WT)

    Plasmid

    #83505

    Purpose

    Bacterial expression of Calreticulin

    Depositor

    Malini Raghavan

    Article

    Del Cid et al J Biol Chem. 2010 Feb 12;285(7):4520-35.

    Insert

    CALR (Calr Mouse)
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

16. pVH016

    Plasmid

    #80400

    Purpose

    No anti-scaffold control with 3xFLAG tags for Western blots. Contains pBAD-CusR (response regulator) and pCusR-GFP(3xFLAG).

    Depositor

    Richard Murray

    Article

    Hsiao et al ACS Synth Biol. 2015 Feb 20;4(2):150-61.

    Inserts

    ffGFP-3xFLAG
    CusR (cusR )
    AraC (araC )
    

    Use

    Synthetic Biology

    Tags

    C-terminal 3xFLAG tag and C-terminal LZx domain

    Expression

    Bacterial

    Available Since

    Sept. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pENTR_L1-BirA(R118G)-NES-YFP-STOP-L2

    Plasmid

    #127357

    Purpose

    gateway entry vector for expressing cytosolic BirA* (R118G)-YFP under a promoter of choice

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    BirA* (R118G mutant)
    

    Use

    Gateway entry vector

    Tags

    GS linker, NES, V5, and YFP

    Mutation

    R118G

    Promoter

    no promoter

    Available Since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. K8.1Pr-pGL4.16

    Plasmid

    #131037

    Purpose

    Firefly Luciferase reporter for K8.1 promoter from KSHV

    Depositor

    Britt Glaunsinger

    Article

    Nandakumar et al PLoS Pathog. 2019 May 16;15(5):e1007774.

    Insert

    KSHV K8.1 100 bp promoter
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1(+) neo with Smad5 CDS

    Plasmid

    #61800

    Purpose

    pcDNA3.1(+) with full length mouse Smad5 CDS

    Depositor

    Judy Lieberman

    Article

    Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

    Insert

    Smad5 CDS (Smad5 Mouse)
    

    Expression

    Mammalian

    Available Since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pcDNA-NES-ZapCY2

    Plasmid

    #59016

    Purpose

    Cytosolic zinc-binding FRET biosensor

    Depositor

    Amy Palmer

    Article

    Qin et al ACS Chem Biol. 2013 Nov 15;8(11):2366-71

    Insert

    NES-ZapCY2 genetically encoded cytosolic Zn(II) sensor
    

    Tags

    Nuclear Exclusion Signal

    Expression

    Mammalian

    Mutation

    See comment

    Promoter

    CMV

    Available Since

    Aug. 21, 2014

    Availability

    Academic Institutions and Nonprofits only