We narrowed to 53,424 results for: plasmid

1. pPTG-GG-sg2+sg3

   Plasmid

   #127199

   Purpose

   Plasmid expressing sgRNA2 (TACCACATTTGTAGAGGTT) & sgRNA3 (CAATGTATCTTATCATGTC) as polycistronic tRNA-gRNA from single U6 promoter

   Depositor

   Jordan Green

   Article

   Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

   Insert

   tRNA-gRNA
   

   Use

   Empty sgrna plasmid

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV/EF1a::DIO-iLMO2

   Plasmid

   #129482

   Purpose

   AAV2 transfer plasmid for Cre-dependent expression of iLMO2, an opto-chemogenetic probe for neuronal inhibition by light or chemical

   Depositor

   Robert Gross

   Article

   Jaiswal et al J Neurosci Res. 2017 Aug 3. doi: 10.1002

   Insert

   iLMO2
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV/CaMKIIa::iLMO2

   Plasmid

   #129481

   Purpose

   AAV2 transfer plasmid for iLMO2, an opto-chemogenetic probe for neuronal inhibition by light or chemical, under control of the glutamatergic-selective CaMKIIa promoter

   Depositor

   Robert Gross

   Article

   Tung et al Sci Rep. 2015 Sep 24;5:14366. doi: 10.10

   Insert

   iLMO2
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CaMKIIa

   Available since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTS2308-Tier1-pGL3-PPGK-NLuc_TB

   Plasmid

   #169551

   Purpose

   Tier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' insulator sequence derived from the pGL3 plasmid (pGL3-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   pGL3-insulator-modulated PPGK-driven NanoLuc Luciferase - mTagBFP expression cassette
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PPGK

   Available since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUDP002

   Plasmid

   #103872

   Purpose

   Broad-host-range Cas9/gRNA co-expression backbone plasmid (no gRNA)

   Depositor

   Jean-Marc Daran

   Article

   Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

   Inserts

   hph
   Spcas9 D147Y P411T
   

   Use

   CRISPR

   Tags

   NLS

   Expression

   Yeast

   Mutation

   D147Y P411T

   Promoter

   Arxula adeninivorans TEF1 and Ashbya gossypii (Er…

   Available since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-HA

   Plasmid

   #128034

   Purpose

   Mammalian expression vector with N terminal HA tag

   Depositor

   Oskar Laur

   Type

   Empty backbone

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-ccdB-Nanoluc

   Plasmid

   #87067

   Purpose

   Mammalian Gateway-compatible expression vector backbone with a C-terminal Nanoluc luciferase

   Depositor

   Mikko Taipale

   Type

   Empty backbone

   Use

   Tags

   Nanoluc luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pXPR_047

   Plasmid

   #107145

   Purpose

   measures Cas9 activity

   Depositor

   David Root

   Insert

   GFP + sgRNA for GFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pICherryNeo

   Plasmid

   #52119

   Purpose

   a bicistronic IRES-mCherry containing vector for mammalian expression (CMV promoter)

   Depositor

   Dario Vignali

   Type

   Empty backbone

   Use

   Tags

   IRES-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-cCFP

    Plasmid

    #185361

    Purpose

    For mammalian expression of C-term CFP for split-CFP

    Depositor

    Ayaz Najafov

    Insert

    CFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFastbac1 Nterminal GST

    Plasmid

    #128058

    Purpose

    Baculovirus expression vector with N terminal GST

    Depositor

    Oskar Laur

    Type

    Empty backbone

    Use

    Tags

    GST

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Nov. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. MSCV Luciferase PGK-hygro

    Plasmid

    #18782

    Depositor

    Scott Lowe

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Luciferase and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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13. LifeAct-mScarletI pDRF1-GW

    Plasmid

    #168085

    Purpose

    Expresses Lifeact-mScarletI to visualize actin in yeast

    Depositor

    Bas Teusink

    Insert

    LifeAct-mScarletI
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pIGneo

    Plasmid

    #52116

    Purpose

    a bicistronic IRES-GFP containing vector for mammalian expression (CMV promoter)

    Depositor

    Dario Vignali

    Type

    Empty backbone

    Use

    Tags

    IRES-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pC-sleeping beauty

    Plasmid

    #65487

    Purpose

    HSP-driven SB100X Sleeping Beauty transposase in pCasper backbone.

    Depositor

    Guillaume Filion

    Insert

    sleeping beauty transposase
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    HSP

    Available since

    Dec. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. WT-eGFP

    Plasmid

    #215168

    Purpose

    Encodes the enhanced green fluorescent protein

    Depositor

    Travis Beddoe, Bhuvana Shanbhag

    Insert

    WT-eGFP
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 lac

    Available since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. FUGW ECFP

    Plasmid

    #128068

    Purpose

    Lentiviral expression of ECFP

    Depositor

    Oskar Laur

    Insert

    ECFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    no

    Promoter

    Available since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pVL1393-6xMyc

    Plasmid

    #128035

    Purpose

    Baculovirus expression vector with C-terminal 6xMyc tag

    Depositor

    Oskar Laur

    Type

    Empty backbone

    Use

    Tags

    6xMyc tag

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Nov. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1-nCFP

    Plasmid

    #185360

    Purpose

    For mammalian expression of N-term CFP for split-CFP

    Depositor

    Ayaz Najafov

    Insert

    CFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    June 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pN1-TOMM20-mScarletI

    Plasmid

    #129346

    Purpose

    Mitochondrial outer membrane localization

    Depositor

    Yasushi Okada

    Insert

    mScarlet(i) (Addgene 85044)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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