We narrowed to 6,558 results for: siae
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Plasmid#104342PurposeExpress yeast Yap5-3xflag and reference flag-DHFR-ha protein, both are contolled by tetracycline mediated TDH promter, test protein expression and stability.DepositorInsertpTDH3tc3-Yap5-3xFLAG-tCyc1-pTDHtc3-flagDHFRha-tCyc1 (YAP5 Budding Yeast)
Tags3xFLAGExpressionYeastAvailable SinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
ISceI-GR-RFP
Plasmid#17654DepositorTagsmRFPExpressionMammalianMutationC655G mutation in the glucocorticoid receptor (se…Available SinceMarch 28, 2008AvailabilityAcademic Institutions and Nonprofits only -
pBDP
Plasmid#217827PurposeStuffer plasmid with golden gate compatible bidirectional promoterDepositorInsertGal1/Gal10 Bidirectional Promoter
UseCloning vectorAvailable SinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTA-Mob 2.0
Plasmid#149662PurposeConjugation helper plasmid for use in cis (self-transfer) and trans (transfer of plasmids with RK2/RP4 origin of transfer) mobilization to prokaryotic and eukaryotic cells.DepositorTypeEmpty backboneUseSynthetic Biology; Conjugation helper plasmidExpressionBacterialAvailable SinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRS415-Cas9-VPR
Plasmid#163971PurposeTrifunctional Cas9-VPR fusion protein plasmid for simultaneous transcriptional activation, transcriptional repression, and genome editing in yeastDepositorInsertCas9-VPR
UseSynthetic BiologyExpressionYeastAvailable SinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKP112 (pRS406-GFP-ADH1t)
Plasmid#106472PurposeVector for creating C-terminal GFP fusion proteins in yeast.DepositorTypeEmpty backboneTagsGFPExpressionBacterial and YeastPromoterNoneAvailable SinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAM57
Plasmid#227654PurposepETDuet-1: Rpn8(1-179)-PreScission-Strep, H6-PreScission-Rpn11(2-239, V80A)DepositorTagsH6-PreScission and PreScission-StrepExpressionBacterialMutation1-179 and amino acids 2-239, V80APromoterT7Available SinceDec. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAM71
Plasmid#227621PurposepETDuet-1 H6-precission-Rpn11(24-160-GGSGSG-192-221) , Rpn8(1-179)DepositorTagsHis-PrescissionExpressionBacterialMutation1-179 and amino acids 24-160-GGSGSG-192-221PromoterT7Available SinceDec. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIAeBlueHPV16LR
Plasmid#185894PurposeIn vivo gene amplification (HapAmp) of AeBlue + HPV16 L1 expression module at RPL25 locusDepositorInsertPRPL25(Arm1)>Kl.LEU2>TKl.LEU2-TRPL25(Arm3)-ARS305-PALD6>EforRed>TPGK1-PSe.GAL2>HPV16-L1_C-6*H>TRPL41B-PBTS1>RPL25(partial;Arm2)
ExpressionYeastMutationNoneAvailable SinceNov. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-ScChd1(118-1274)
Plasmid#221207PurposeScChd1-aa118-1274 chromatin remodelerDepositorInsertScChd1 (aa118-1274)
Tags6xHis-tag (Precission protease cleavable)ExpressionBacterialMutationdeleted amino acids 1-117 and 1275-1468Available SinceAug. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-His6-E3(1)-CUbo-TwinStrep
Plasmid#212712PurposeExpression of His6-E3(1)-CUbo-TwinStrep in insect cellsDepositorInsertE3(1)
Tags6xHis and CUbo-TwinStrepExpressionBacterialAvailable SinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDS275
Plasmid#34972PurposeExpresses Mid2(SS/TM), containing only the signal sequence and transmembrane helix, fused to GFP and LOVpep(T406A, T407A, I532A mutations).DepositorInsertMid2(SS/TM)-GFP-LOVpep
ExpressionYeastMutationT406A, T407A, I532AAvailable SinceMarch 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCSIIbsr-6085x
Plasmid#108653PurposeA tyrosine kinase biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)DepositorInsertPicchuEV
UseLentiviralTagsXFPExpressionMammalianAvailable SinceApril 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCTCON2 Aga2P-HRP
Plasmid#73152PurposeFull-length horseradish peroxidase (HRP) fused to the C-terminus of the yeast mating protein Aga2P. Galactose-inducible expression.DepositorInsertAga2P-HRP
TagsHA tag and myc tagExpressionYeastPromoterGAL1Available SinceJune 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-FLAG-APEX2-NES-NatMX6
Plasmid#229231PurposeTemplate plasmid for the endogenous tagging with FLAG-APEX2-NAS in yeastDepositorTypeEmpty backboneExpressionYeastAvailable SinceFeb. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCRCT
Plasmid#60621PurposePlasmid encoding iCas9, tracrRNA and crRNAsDepositorInsertsiCas9
tracrRNA
UseCRISPRTagsFLAG and SV40 NLSExpressionYeastMutationchanged Aspartate 147 to Tyrosine, Proline 411 to…PromoterRPR1p and TEF1pAvailable SinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn1-DIO-Flpo
Plasmid#237222PurposeFor Cre-dependent expression of Flp recombinaseDepositorInsertFlpo recombinase
UseAAV and Cre/LoxExpressionMammalianPromoterhSynAvailable SinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSJ1256 (pFA6-link-yGFP1-10-CaURA3MX)
Plasmid#86419PurposeC-terminal PCR tagging module for yeast codon optimized split GFP1-10DepositorInsertGFP1-10
ExpressionYeastAvailable SinceFeb. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-GFP(S65T)-His3MX6
Plasmid#41598DepositorInsertHis3
UseYeast genomic targetingTagsA. gossypii translation elongation factor 1a gene…ExpressionYeastPromoterTEFAvailable SinceMay 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
HDAC5 Flag
Plasmid#13822PurposeMammalian expression of human histone deacetylase 5 with flag tagDepositorAvailable SinceFeb. 19, 2007AvailabilityAcademic Institutions and Nonprofits only