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pcDNA3-endo-GsCT

Plasmid

#205516

Purpose

Endosome-targeted C-terminal fragment of human Gαs protein (Gαs inhibitory peptide).

Depositor

Jin Zhang

Article

Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:

Insert

endo-GsCT (GNAS Human, Synthetic)

Tags

2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger) and mChe…

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 27, 2023

Availability

Academic Institutions and Nonprofits only

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PB_TetON_mCherry_P2AT2A_GFP-nb-2xKS_FtoG

Plasmid

#238244

Purpose

For integration of mCherry_P2AT2A_GFP-nb-2xKS_FtoG

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

mCherry_P2AT2A_GFP-nb-2xKS_FtoG

Expression

Mammalian

Available Since

June 4, 2025

Availability

Academic Institutions and Nonprofits only

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ΔNp63beta

Plasmid

#27014

Depositor

David Sidransky

Insert

ΔNp63beta (TP63 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 25, 2011

Availability

Academic Institutions and Nonprofits only

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pLPC-Myc-BirA-TRF1

Plasmid

#234040

Purpose

Expresses Myc-His-BirA(R118G)-TRF1

Depositor

Roderick O'Sullivan

Article

Garcia-Exposito et al Cell Rep. 2016 Nov 8;17(7):1858-1871. do

Insert

TRF1 (TERF1 Human)

Use

Retroviral

Tags

Myc-6xHis-BirA(R118G)

Expression

Mammalian

Available Since

Aug. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pOttc556 - pAAV EF1a iCre

Plasmid

#210416

Purpose

AAV viral vector packaging plasmid that expresses "improved Cre recombinase"

Depositor

Brandon Harvey, Christopher Richie

Insert

iCre

Use

AAV

Promoter

EF1a

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pBabe-Dvl2-GFP

Plasmid

#109148

Purpose

Stable Dvl2 expression

Depositor

Mariann Bienz

Article

Gammons et al J Cell Sci. 2016 Oct 15;129(20):3892-390

Insert

Dvl2 (DVL2 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Promoter

SV40

Available Since

July 2, 2018

Availability

Academic Institutions and Nonprofits only

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pHR_dSV40-Aurora A-GFP

Plasmid

#67924

Purpose

Expresses Aurora A-GFP in mammalian cells

Depositor

Ron Vale

Article

Tanenbaum et al Elife. 2015 Aug 25;4. doi: 10.7554/eLife

Insert

GFP-Aurora A (AURKA Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pCDH_Str-Ii

Plasmid

#65313

Purpose

Cytoplasmic ER hook only (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Invariant chain (p33)

Use

Lentiviral

Mutation

Streptavidin in its cytoplasmic part

Promoter

CMV

Available Since

Aug. 26, 2015

Availability

Academic Institutions and Nonprofits only

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pRK5-myc-Miro2

Plasmid

#47891

Purpose

Expresses myc tagged Miro2

Depositor

Pontus Aspenström

Article

Fransson et al J Biol Chem. 2003 Feb 21;278(8):6495-502

Insert

Miro2 (RHOT2 Human)

Tags

myc

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only

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p3E_BAG3wt-eGFP

Plasmid

#98180

Purpose

Codes for full length, GFP tagged, human wildtype Bag3

Depositor

Robert Bryson-Richardson

Article

Ruparelia et al Acta Neuropathol. 2014 Dec;128(6):821-33

Insert

BAG3wt-eGFP (BAG3 Human)

Use

Gateway vector

Tags

GFP

Available Since

June 30, 2017

Availability

Academic Institutions and Nonprofits only

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TRPC1-FLAG

Plasmid

#24408

Depositor

Craig Montell

Article

Xu et al Cell. 1997 Jun 27. 89(7):1155-64.

Insert

TRPC1 (TRPC1 Human)

Tags

Flag

Expression

Mammalian

Available Since

Aug. 20, 2010

Availability

Academic Institutions and Nonprofits only

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pLenti_gRNA -hMLHdn-Puro

Plasmid

#191105

Purpose

Backbone for lentiviral pegRNA library construction with hMLH1dn and Puro marker

Depositor

Hyongbum Kim

Article

Yu et al Cell. 2023 Apr 21:S0092-8674(23)00331-8.

Insert

hMLH1dn

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

I31 silent mutation (ATA to ATC) to remove early …

Promoter

EF-1a

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-3*Flag-APEX2 N-term NONO

Plasmid

#187580

Purpose

Express FLAG epitope and APEX2-tagged NONO fusion protein in mammalian cells

Depositor

Benjamin Blencowe

Article

Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

Insert

NONO (NONO Human)

Tags

APEX2-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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TFORF1159

Plasmid

#144273

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

EOMES (EOMES Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC4A7

Plasmid

#132204

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC4A7 (SLC4A7 Human)

Expression

Mammalian

Available Since

Oct. 28, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pHCD1B

Plasmid

#102953

Purpose

mammalian expression of CD1B

Depositor

Gennaro De Libero, Lucia Mori

Article

Manolova et al Eur J Immunol. 2003 Jan;33(1):29-37.

Insert

CD1B cDNA (CD1B Human)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 18, 2018

Availability

Academic Institutions and Nonprofits only

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TFORF1916

Plasmid

#144317

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

RUNX2 (RUNX2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pET-28a-mCherry-MED1-IDR

Plasmid

#194545

Purpose

Bacterial Expression of 6xHis mCherry-MED1-IDR Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

MED1 (MED1 Human)

Tags

mCherry

Expression

Bacterial

Available Since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

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TDP-43 ΔNTD GFP

Plasmid

#237546

Purpose

Expression of GFP-tag fused to mutant TDP-43 ΔNTD

Depositor

Tatyana Shelkovnikova

Insert

GFP-tagged TDP-43 ΔNTD (TARDBP Human)

Tags

EGFP

Expression

Mammalian

Mutation

TDP-43 1-90 a.a. deletion (TDP-43 35kDa)

Promoter

CMV

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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mNeonGreen-peroxi

Plasmid

#137806

Purpose

Visualization of peroxisomes

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

SKL

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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