58,349 results

1. pENTR-actin-R62D

   Plasmid

   #118379

   Purpose

   Gateway compatible donor vector with actin-R62D.

   Depositor

   Maria Vartiainen

   Article

   Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

   Insert

   actin beta (ACTB Human)
   

   Use

   Donor-plasmid

   Tags

   Expression

   Mutation

   R62D

   Promoter

   Available since

   Nov. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHAGE-3FLAG-TFR1

   Plasmid

   #176490

   Purpose

   For lentiviral transduction of 3FLAG-TFR1

   Depositor

   Wade Harper

   Article

   Park et al Nat Commun. 2022 Oct 16;13(1):6112. doi:

   Insert

   TFR1 (TFRC Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   Available since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. WWP-Luc (p21/WAF1 promoter)

   Plasmid

   #16451

   Depositor

   Bert Vogelstein

   Article

   el-Deiry et al Cell. 1993 Nov 19. 75(4):817-25.

   Insert

   WAF1 promoter (CDKN1A Human)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 11, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEX6P1‐hsRNASEH2BCA

   Plasmid

   #108692

   Purpose

   For expression in E coli of N-terminally GST-tagged human RNASEH2B and untagged RNASEH2C and A, allowing affinity purification of the RNase H2 trimeric enzyme

   Depositor

   Andrew Jackson, Martin Reijns

   Article

   Reijns et al J Biol Chem. 2011 Mar 25;286(12):10530-9

   Inserts

   RNASEH2B (RNASEH2B Human)
   RNASEH2C (RNASEH2C Human)
   RNASEH2A (RNASEH2A Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Shine Dalgarno sequence upstream of start codon a…

   Promoter

   tac

   Available since

   April 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. EYFP WC visual barcode

   Plasmid

   #158675

   Purpose

   tagging clonal populations with whole cell EYFP fluorescent protein

   Depositor

   Ravid Straussman

   Article

   Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

   Insert

   EYFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDNA5/FRT/TO_TOMM20_HaloTag9_T2A_EGFP

   Plasmid

   #169333

   Purpose

   Expression of HaloTag9 at the mitochondrial surface of mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   TOMM20-HaloTag9 (TOMM20 Human)
   

   Use

   Tags

   HaloTag9

   Expression

   Mammalian

   Mutation

   HaloTag9 = HaloTag7-Q165H-P174R

   Promoter

   Available since

   Nov. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. MAC_C_CH60

   Plasmid

   #111662

   Purpose

   MAC-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

   Insert

   CH60 (HSPD1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. Integrin alpha5-AHCys

   Plasmid

   #27313

   Depositor

   Timothy Springer

   Article

   Takagi et al Nat Struct Biol. 2001 May . 8(5):412-6.

   Insert

   integrin alpha 5 (ITGA5 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   integrin alpha5 (1-954) + AHCys

   Promoter

   Available since

   March 8, 2011

   Availability

   Academic Institutions and Nonprofits only

9. mEmerald-Mito-7

   Plasmid

   #54160

   Purpose

   Localization: Mitochondria, Excitation: 487, Emission: 509

   Depositor

   Michael Davidson

   Article

   Planchon et al Nat Methods. 2011 May;8(5):417-23. doi:

   Insert

   Mito (COX8A Human)
   

   Use

   Tags

   mEmerald

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 16, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. MS2_adRNA-MCP_ADAR2 DD (E488Q)_NES

    Plasmid

    #124707

    Purpose

    Expresses MS2_adRNA targeting the RAB7A transcript along with the MCP_ADAR2 DD(E488Q)_NES

    Depositor

    Prashant Mali

    Article

    Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

    Insert

    MCP_ADAR2 DD (E488Q)_NES
    

    Use

    AAV

    Tags

    NES

    Expression

    Mammalian

    Mutation

    E488Q hyperactive mutant

    Promoter

    CMV

    Available since

    Sept. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-BEACON2

    Plasmid

    #171698

    Purpose

    Expresses BEACON2 in mammalian cells

    Depositor

    Jia Chen

    Article

    Wang et al Cell Rep. 2020 Jun 2;31(9):107723. doi:

    Insert

    BEACON2
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    hAPOBEC3A_W98Y/W104A/Y130F

    Promoter

    CMV

    Available since

    Aug. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-CD68 promoter/enhancer

    Plasmid

    #34837

    Depositor

    Peter Murray

    Article

    Lang et al J Immunol. 2002 Apr 1;168(7):3402-11.

    Insert

    CD68 promoter/enhancer -680/+140 (CD68 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3 HA human NEMO

    Plasmid

    #13512

    Depositor

    Kunliang Guan

    Article

    Tang et al J Biol Chem. 1999 Jun 11. 274(24):16741-

    Insert

    NEMO (IKBKG Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 11, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. Human PNP his-tagged

    Plasmid

    #64076

    Purpose

    Expression of human Purine Nucleoside Phosphorylase

    Depositor

    Vern Schramm

    Article

    Lewandowicz et al J Biol Chem. 2003 Aug 22;278(34):31465-8

    Insert

    Purine Nucleoside Phosphorylase (PNP Human)
    

    Use

    Tags

    Histidine tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pMIR-DsRed-IRES-His-Halo-Tev-Keap1

    Plasmid

    #58240

    Purpose

    expresses halo tagged human Keap1 protein

    Depositor

    Yimon Aye

    Article

    Fang et al J Am Chem Soc. 2013 Oct 2;135(39):14496-

    Insert

    Kelch like ECH associated protein 1 (KEAP1 Human)
    

    Use

    Tags

    His-Halo-Tev

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pLEX307-DPP4-puro

    Plasmid

    #158451

    Purpose

    Constitutive lentiviral expression of human DPP4.

    Depositor

    Sho Iketani, Alejandro Chavez

    Insert

    DPP4 (DPP4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pC0050-CMV-dPspCas13b-longlinker-ADAR2DD(wt)

    Plasmid

    #103866

    Purpose

    dPspCas13b-ADAR2DD(wt) fusion that can be used to selectively edit adenosine to inosine in RNA molecules when used in conjuction with a guide RNA. Longer linker than pC0039.

    Depositor

    Feng Zhang

    Article

    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

    Inserts

    PspCas13b
    huADAR2DD (ADARB1 Human)
    

    Use

    CRISPR

    Tags

    GSGGGGS and HIV NES

    Expression

    Mammalian

    Mutation

    Changed histidne 133 to alanine and histidine 105…

    Promoter

    Available since

    Nov. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. A*66:01:01

    Plasmid

    #203291

    Purpose

    Overexpress HLA allele

    Depositor

    Derin Keskin, Catherine Wu

    Article

    Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

    Insert

    A*66:01:01 (HLA-A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-3XFLAG-hINO80

    Plasmid

    #44149

    Depositor

    Joan Conaway

    Article

    Chen et al J Biol Chem. 2011 Apr 1;286(13):11283-9.

    Insert

    hINO80 full length (INO80 Human)
    

    Use

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGEX-EZH2

    Plasmid

    #28060

    Depositor

    Mien-Chie Hung

    Article

    Wei et al Nat Cell Biol. 2011 Jan . 13(1):87-94.

    Insert

    EZH2 (EZH2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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