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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 28,416 results for: REP

Showing: 7651 - 7680 of 28416 results

  2. pCVL.SSA TLR (Sce)

    Plasmid
    #45576
    Purpose
    Codes for the SSA-TLR with the target site for I-Sce I in a lentiviral backbone
    Depositor
    Andrew Scharenberg
    Article
    Kuhar et al Nucleic Acids Res. 2013 Oct 10.
    Inserts
    5' iRFP arm
    eGFP with I-Sce I TS
    +3 mCherry
    3' iRFP arm
    Use
    Lentiviral
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    embedded I-Sce I TS from 163-185bp, truncated 25 …
    Promoter
    SFFV
    Available since
    Nov. 4, 2013
    Availability
    Academic Institutions and Nonprofits only
    45576-plasmid-map-sequence-id-67850

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  3. pBAD-bHs

    Plasmid
    #134259
    Purpose
    For L-arabinose inducible bacterial expression of S. typhimurium codon-optimized hyaluronidase (Streptomyces koganeinsis UniProt A0A0U2E2) with N-terminal 6x His tag
    Depositor
    Edwin Manuel
    Article
    Ebelt et al Mol Cancer Ther. 2019 Nov 6. pii: 1535-7
    Insert
    Hyaluronidase from Streptomyces koganeinsis codon-optimized for Salmonella typhimurium
    Use
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    codon optimized for Salmonella typhimurium
    Promoter
    AraC
    Available since
    Nov. 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    134259-plasmid-map-sequence-id-264461

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  4. pLV[Exp]-Neo-EF1A>Tet3G

    Plasmid
    #184379
    Purpose
    The Tet3G gene is expressed under a constitutive EF1-alpha promoter. This protein binds a TRE3G promoter to activate gene transcription only in the presence of tetracycline or its analogs (e.g. doxycycline)
    Depositor
    Ori Bar-Nur
    Article
    Domenig et al Stem Cell Reports. 2022 Feb 8;17(2):321-
    Insert
    Tet3G
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    184379-plasmid-map-sequence-id-362511

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  7. pNAD1

    Plasmid
    #154347
    Purpose
    pUC19 backbone with editing template for homologous recombination mediated deletion of Nitrate reductase gene of N. oceanica and selection based on zeocin resistance
    Depositor
    John van der Oost
    Article
    Naduthodi et al Biotechnol Biofuels. 2019 Mar 25;12:66.
    Insert
    ShBle
    Use
    Microalgae
    Tags
    Expression
    Bacterial
    Mutation
    none
    Promoter
    Violaxanthin Chlorophyll a Binding Protein Precur…
    Available since
    Nov. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    154347-plasmid-map-sequence-id-316607

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  8. pBeta-lacZ-attBx2

    Plasmid
    #124445
    Purpose
    Plasmid for in vivo enhancer reporter assay. Plasmid contains a β-globin minimal promoter followed by a lacZ reporter gene derived from pRS16, with the entire reporter cassette flanked by attB sites.
    Depositor
    Yingguang Frank Chan
    Article
    Castro et al Elife. 2019 Jun 6;8:e42014. doi: 10.7554
    Type
    Empty backbone
    Use
    Enhancer reporter assay construct
    Tags
    LacZ
    Expression
    Mammalian
    Mutation
    Promoter
    beta globin minimal promoter
    Available since
    May 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    124445-plasmid-map-sequence-id-242276

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  10. SpIMPDH

    Plasmid
    #206980
    Purpose
    Streptococcus pyogenes Inosine 5′-Monophosphate Dehydrogenase (SpIMPDH, EC. 1.1.1.205). Contains His6 tag with TEV protease cleavage site in pET-28b(+) plasmid for protein expression.
    Depositor
    Vern Schramm
    Article
    Glockzin et al Biochemistry. 2022 Oct 4;61(19):2088-210
    Insert
    SpIMPDH (guaB Streptococcus pyogenes)
    Use
    Tags
    His tag (with TEV cleavage site)
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    206980-plasmid-map-sequence-id-410397

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  18. pCVL.SSA TLR (Ani)

    Plasmid
    #45577
    Purpose
    Codes for the SSA-TLR with the target site for I-Ani I in a lentiviral backbone
    Depositor
    Andrew Scharenberg
    Article
    Kuhar et al Nucleic Acids Res. 2013 Oct 10.
    Inserts
    5' iRFP arm
    eGFP with I-Ani I TS
    +3 mCherry
    3' iRFP arm
    Use
    Lentiviral
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    embedded I-Ani I TS from 163-185bp, truncated 25 …
    Promoter
    SFFV
    Available since
    Nov. 4, 2013
    Availability
    Academic Institutions and Nonprofits only
    45577-plasmid-map-sequence-id-67848

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  23. pRAR104

    Plasmid
    #185034
    Purpose
    400 nt l31 promoter region, l31 5'UTR A74 and A76 deletion, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB
    Depositor
    Julius Lucks
    Article
    Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127
    Insert
    L31-sfGFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    A74 and A76 deletion of rpmE 5'UTR, Only fir…
    Promoter
    Available since
    July 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    185034-plasmid-map-sequence-id-366945

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  24. pRAR105

    Plasmid
    #185035
    Purpose
    400 nt l31 promoter region, l31 5'UTR G52 and G79 deletion, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB
    Depositor
    Julius Lucks
    Article
    Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127
    Insert
    L31-sfGFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    G52 and G79 deletion of rpmE 5'UTR, Only fir…
    Promoter
    Available since
    July 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    185035-plasmid-map-sequence-id-363329

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  25. pRAR107

    Plasmid
    #185037
    Purpose
    400 nt l31 promoter region, l31 5'UTR AGA74-76GAG mutation, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB
    Depositor
    Julius Lucks
    Article
    Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127
    Insert
    L31-sfGFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    AGA74-76GAG mutation of rpmE 5'UTR, Only fir…
    Promoter
    Available since
    July 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    185037-plasmid-map-sequence-id-363330

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  26. pRAR109

    Plasmid
    #185038
    Purpose
    400 nt l31 promoter region, l31 5'UTR G60 and C69 deletion, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB
    Depositor
    Julius Lucks
    Article
    Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127
    Insert
    L31-sfGFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    G60 and C69 deletion of rpmE 5'UTR, Only fir…
    Promoter
    Available since
    July 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    185038-plasmid-map-sequence-id-363331

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  27. pRAR110

    Plasmid
    #185039
    Purpose
    400 nt l31 promoter region, l31 5'UTR G62 and G67 deletion, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB
    Depositor
    Julius Lucks
    Article
    Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127
    Insert
    L31-sfGFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    G62 and G67 deletion of rpmE 5'UTR, Only fir…
    Promoter
    Available since
    July 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    185039-plasmid-map-sequence-id-363604

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Showing: 7651 - 7680 of 28416 results