We narrowed to 13,644 results for: NTS
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Plasmid#71257Purposeluciferase reporter with 3 copies of the GM420 NFAT-AP-1 site in front of hGM-CSF -55 to +28 minimal promoterDepositorInsert3 copies of the GM420 NFAT-AP-1 site (CSF2 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterGM-CSF minimalAvailable sinceDec. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLM-vexGFP-Oct4
Plasmid#22240Purpose2nd generation lentiviral vectorDepositorInsertvexGFP_2A_OCT4 (POU5F1 Human, synthetic fluorescent protein)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
pYPQ-CBE-SpRYn-Act3.0
Plasmid#178958PurposeIt consists of a SpRY nickase (D10A) fused with a cytidine deaminase (A3A/Y130F) and MS2 -SunTag-activators (ScFv-sfGFP-2xTAD), enabling simultaneous C to T conversion and gene activation.DepositorInserthA3A-SpRY-UGI-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBT2-4xUAS:P2A-EGFP
Plasmid#127591PurposeThis vector is used for cloning a gene driven by 4xnrUAS, to be co-expressed with EGFP through P2A self-cleaving peptides.DepositorTypeEmpty backboneUseZebrafish expressionTagsExpressionMammalianMutationPromoterAvailable sinceSept. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYPQ166-SpRY
Plasmid#161520PurposeGateway entry plasmid (attL1 & attR5) expressing 3_FLAG-NLS-zSpRYCas9-NLS without promoterDepositorInsertzSpRYCas9
UseCRISPR; Gateway compatible zsprycas9 entry cloneTags3X FLAG, NLS and NLSExpressionPlantMutationPromoterAvailable sinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_GLP1R-NTEV-TCS-GV-2xHA
Plasmid#194378PurposeSplit TEV assaysDepositorInsertGLP1R-NTEV-TCS-GV-2xHA (GLP1R Synthetic, Human)
UseTags2xHAExpressionMammalianMutationPromoterCMVAvailable sinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
6xHis-MBP-BrCas12b
Plasmid#182276PurposeExpresses recombinant BrCas12bDepositorInsertBrCas12b
UseCRISPR and Synthetic BiologyTags6xHis, T7, Thrombin siteExpressionBacterialMutationPromoterT7Available sinceApril 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
EGFR D837A
Plasmid#11014PurposeRetroviral construct for expressing human EGFR with D837A mutation in human cellsDepositorInsertEGFR D837A (EGFR Human)
UseRetroviralTagsExpressionMammalianMutationD837APromoterAvailable sinceDec. 2, 2005AvailabilityAcademic Institutions and Nonprofits only -
pCD565
Plasmid#153027PurposedxCas9(3.7), MCP-SoxS(R93A/S101A), J306 scRNADepositorInsertdxCas9(3.7), MCP-SoxS(R93A/S101A), J306 scRNA
UseCRISPRTagsExpressionBacterialMutationSoxS has R93A and S101A mutationsPromoterAvailable sinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMOD_C2200
Plasmid#91082PurposeModule C, Promoter: none – to be combined with gRNA array in module B, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers (only works with pMOD_B2203), Terminator: 35SDepositorInsertSapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers
UseCRISPRTagsExpressionMutationPromoternone (will be fused to gRNA array in MODULE B)Available sinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYPQ-ABE-SpRYn-Act3.0
Plasmid#178959PurposeIt consists of a SpRY nickase (D10A) fused with an adenine deaminase (ABE8e) and MS2-SunTag-activators (ScFv-sfGFP-2xTAD), enabling simultaneous A to G conversion and gene activation.DepositorInsertABE8e-SpRY-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/TO-N-mEGFP-PARP11
Plasmid#177998PurposeExpresses N-terminally mEGFP-tagged PARP11DepositorInsertPARP11 (PARP11 Human)
UseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
Lenti_PGK-Cas9-P2A-HygR
Plasmid#164135PurposeLentiviral construct for the expression of SpCas9 driven by PGK promoter in mammalian cellsDepositorInsertSpCas9 (cas9 Synthetic)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterPGKAvailable sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHR Grb2-TagBFP
Plasmid#188631PurposeHuman Grb2 C-terminally labeled with TagBFPDepositorInsertGRB2 (GRB2 Synthetic, Human)
UseLentiviralTagsTagBFPExpressionMammalianMutationPromoterSFFVAvailable sinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBSFI-myr-akt-T308A/S473A
Plasmid#49191PurposecDNA of Akt1 with myristyolation signal and point mutation from T to A at position 308 and S to A at position 473 in cloning vectorDepositorInsertAkt1 (Akt1 Mouse)
UseAdaptor plasmidTagsHA tag and myristolation signalExpressionMutationT308A; S473APromoterT7Available sinceDec. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLM-mCitrine-Sox2
Plasmid#23242DepositorInsertmCitrine_2A_SOX2 (SOX2 Human, synthetic fluorescent protein)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
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LentiU6-hASCL1 gRNA_1-MS2-Puro
Plasmid#192670PurposeLentiviral expression of sgRNA targeting hASCL1 promoter to activate human ASCL1 transcriptionDepositorInsertHuman ASCL1 activating gRNA #1 (ASCL1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBSFI-myr-akt-T308D/S473D
Plasmid#49192PurposecDNA of Akt1 with myristyolation signal and point mutation from T to D at position 308 and S to D at position 473 in cloning vectorDepositorInsertAkt1 (Akt1 Mouse)
UseAdaptor plasmidTagsHA tag and myristolation signalExpressionMutationT308D; S473DPromoterT7Available sinceDec. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
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FSW-HRP-V5-LRRTM2
Plasmid#82537PurposeLentiviral vector for expressing HRP-LRRTM2 in neuronsDepositorInsertLRRTM2 (LRRTM2 Human)
UseLentiviralTagsHRP and V5ExpressionMammalianMutationPromoterHuman SynapsinAvailable sinceSept. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
Lenti_CMV-Cas9-P2A-HygR
Plasmid#164133PurposeLentiviral construct for the expression of SpCas9 driven by CMV promoter in mammalian cellsDepositorInsertSpCas9 (cas9 Synthetic)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
CoV2-N-B.1.1.7
Plasmid#177958PurposeTransient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (D3L, R203K, G204R, S235F). Used for generating RNA packaging virus-like particles.DepositorInsertSARS-CoV-2 Nucleocapsid (D3L, R203K, G204R, S235F) (N )
UseTagsExpressionMammalianMutationD3L,R203K,G204R,S235FPromoterAvailable sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ265E6
Plasmid#213469PurposeGateway entry clone (attL1 & attR5) TadA-CDa-32aa linker-zSpCas9-D10A-2xUGI for C-T base editingDepositorInsertTadA-CDa-32aa linker-zSpCas9-D10A-2xUGI
UseCRISPR; Gateway compatible tada-cda-32aa linker-z…TagsNoExpressionPlantMutationPromoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-GST-DAXX-His
Plasmid#175781PurposeDAXX protein for baculovirus expression systemDepositorInsertdeath domain associated protein (DAXX Human)
UseTagsGST and TEVExpressionInsectMutationPromoterAvailable sinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
mH2A2-CT-GFP
Plasmid#45171DepositorUseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceJuly 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 LIC 6A FLAG-TEV-DPP9S
Plasmid#166794PurposeMammalian cell expression vector for Flag-tagged DPP9SDepositorInsertDPP9 (DPP9 Human)
UseTagsFLAG-TEVExpressionMammalianMutationPromoterCMVAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Puro-TDP-43-K263E
Plasmid#133758PurposeLentiviral expression of human TDP-43 K263E disease mutant in mammalian cells.DepositorInsertTDP-43 (TARDBP Human)
UseLentiviralTagsExpressionMammalianMutationChanged lysine 263 to glutamate.PromoterCMVAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_Zeo_ARRB2-1-383-CTEV-2xHA
Plasmid#194382PurposeSplit TEV assaysDepositorInsertARRB2-CTEV-2xHA (ARRB2 Synthetic, Human)
UseTags2xHAExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYPQ262-ABE8e
Plasmid#161523PurposeGateway entry clone (attL1 & attR5) for CRISPR-zSpCas9 ABE8e mediated A-G base editingDepositorInsertABE8e-zSpCas9(D10A)
UseCRISPR; Gateway compatible abe8e-zspcas9(d10a) en…TagsExpressionPlantMutationPromoterAvailable sinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only