We narrowed to 13,832 results for: Cre

Showing: 5121 - 5140 of 13832 results

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pRS416 Gal G294A YFP

Plasmid

#27448

Depositor

Aaron Gitler

Publication

Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

Insert

TDP-43 (TARDBP Human)

Tags

YFP

Expression

Yeast

Mutation

Glycine 294 mutated to Alanine (G294A)

Available since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only
pHvL1P3GA

Plasmid

#112027

Purpose

Expresses sgRNA in barley

Depositor

Wendy Harwood

Publication

Lawrenson et al Methods Mol Biol. 2019;1900:217-232. doi

Insert

LacZ

Use

Unspecified

Tags

None

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pEYK3.1

Plasmid

#17366

Depositor

George Daley

Publication

Koh et al Nucleic Acids Res. 2002 Dec 15. 30(24):e

Insert

GFP

Use

Retroviral

Available since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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pDUET-1-alpha-synuclein-mCerulean3-His6

Plasmid

#110061

Purpose

For bacterial expression of human alpha-synuclein, carboxyl terminally tagged with mCerulean3 and poly-histidines

Depositor

Dan Mulvihill

Publication

Eastwood et al FEBS Lett. 2017 Feb 18. doi: 10.1002/187

Insert

alpha-synuclein (SNCA Human)

Tags

6 x His and mCerulean3

Expression

Bacterial

Promoter

T7

Available since

May 29, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pPB-EF1a-MegaGate-HA-Blast

Plasmid

#175270

Purpose

MegaGate destination with C-terminal HA tag and blasticidin resistance

Depositor

George Church

Publication

Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

Type

Empty backbone

Tags

HA

Expression

Mammalian

Promoter

EF1a

Available since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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ATF4 10: 5’ATF4.uORF1.GFP

Plasmid

#21857

Depositor

David Ron

Publication

Lu et al J Cell Biol. 2004 Oct 11. 167(1):27-33.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ATF4 (Atf4 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

upstream ORF1 (uORF) of ATF4 fused to EGFP

Available since

Sept. 10, 2009

Availability

Academic Institutions and Nonprofits only

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HotPETase_pET21b

Plasmid

#215407

Purpose

Expression construct for HotPETase gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Publication

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

HotPETase

Expression

Bacterial

Mutation

S121E/D186H/R280A/P181V/S207R/S214Y/Q119K/S213E/N…

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLV hU6-gRNA hUbC-VP64-dSaCas9-VP64-T2A-Thy1.1

Plasmid

#194279

Purpose

Expresses gRNA and VP64-dSaCas9-VP64 from lentiviral vector

Depositor

Charles Gersbach

Publication

McCutcheon et al bioRxiv. 2023 May 1:2023.05.01.538906. d

Inserts

humanized VP64 dSaCas9 VP64 T2A Thy1.1
gRNA

Use

CRISPR and Lentiviral

Tags

HA

Expression

Mammalian

Promoter

hU6 and hUbC

Available since

Feb. 16, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET21b Hot-PETase

Plasmid

#225761

Purpose

Expresses codon-optimized Hot-PETase in E. coli with a C-terminal his-tag

Depositor

Jason Boock

Publication

Carter et al Microb Cell Fact. 2024 Nov 25;23(1):319.

Insert

Hot-PETase

Tags

6xHis Tag

Expression

Bacterial

Promoter

T7

Available since

Jan. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pECE-M2-PAK2 S20A

Plasmid

#31681

Depositor

Anne Brunet

Publication

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

p21-activated protein kinase 2 S20A (PAK2 Human)

Tags

M2

Expression

Mammalian

Mutation

changed Serine 20 to Alanine

Available since

April 11, 2012

Availability

Academic Institutions and Nonprofits only

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pOPINNUSA

Plasmid

#41119

Depositor

Ray Owens

Publication

Bird et al Methods. 2011 Sep;55(1):29-37. Epub 2011

Type

Empty backbone

Expression

Bacterial, Insect, and Mamm…

Available since

March 27, 2013

Availability

Academic Institutions and Nonprofits only

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pGWB440

Plasmid

#74826

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with eYFP (no promoter).

Depositor

Tsuyoshi Nakagawa

Publication

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Available since

Sept. 29, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGWB516

Plasmid

#74858

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with 4xMyc (no promoter).

Depositor

Tsuyoshi Nakagawa

Publication

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Available since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

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pTT3 Unc5C-FLAG

Plasmid

#72196

Purpose

Expresses full-length Unc5c with a C-terminal FLAG tag

Depositor

Woj Wojtowicz

Publication

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Unc5c (Unc5c Mouse)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCIG-V5-Snail2-IRES-nls-GFP

Plasmid

#44282

Depositor

Martin Cheung

Publication

Liu et al Proc Natl Acad Sci U S A. 2013 Feb 19;11

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

V5-Snail2 (SNAI2 Chicken)

Tags

V5

Expression

Mammalian

Promoter

CMV-IE-enhancer and chicken beta-actin promoter

Available since

Aug. 19, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pGWB445

Plasmid

#74831

Purpose

Gateway cloning compatible binary vector for N-terminal fusion with eCFP (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Publication

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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SPS-6803

Plasmid

#80458

Purpose

IPTG inducible expressoin of sucrose phosphate synthase derived from Synechocystis sp. PCC6803

Depositor

Daniel Ducat

Publication

Abramson et al Plant Cell Physiol. 2016 Oct 13. pii: pc

Insert

Sucrose phosphate synthase

Expression

Bacterial

Available since

Oct. 25, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti_Ef1a:PE2-P2A-GFP

Plasmid

#184445

Purpose

Single EF1a-driven PE2-P2A-GFP (Addgene #132776) and PaqCI restriction sites for pegRNA cloning. Lentiviral backbone and AmpR.

Depositor

Bobby Koeleman

Publication

Dirkx et al BMC Biol. 2023 Jul 13;21(1):156. doi: 10

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 and Ef1A

Available since

Oct. 18, 2023

Availability

Academic Institutions and Nonprofits only

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HT103_FCMV_QuasAr6a_Citrine

Plasmid

#178822

Purpose

Expresses an archaerhodopsin-derived genetically encoded voltage indicator QuasAr6a carrying a Citrine expression tag

Depositor

Adam Cohen

Publication

Tian et al Nat Methods. 2023 Jul;20(7):1082-1094. d

Insert

QuasAr6a_Citrine

Use

Lentiviral

Tags

citrine

Expression

Mammalian

Promoter

CMV

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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PA-DAD-dark

Plasmid

#53096

Purpose

Expresses dark mutant PA-DAD in mammalian cells, an optogenetic tool to activate endogenous diaphanous related formins in live cells using light with LOV2 in the closed conformation

Depositor

Klaus Hahn, Ronen Zaidel-Bar

Publication

Rao et al Cytoskeleton (Hoboken). 2013 Jul;70(7):3

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

LOV(dark)-6aa-DAD

Tags

6x His and mVenus

Expression

Bacterial, Insect, and Mamm…

Mutation

L47F and C49S in Venus; C450M mutation in LOV2

Available since

Aug. 19, 2015

Availability

Academic Institutions and Nonprofits only

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