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pMJA065

Plasmid

#128517

Purpose

Expresses Ber e 1 in Pichia

Depositor

Marcos Alcocer

Article

Alcocer et al J Mol Biol. 2002 Nov 15;324(1):165-75.

Insert

Ber e 1 a allergenic 2S albumin from Brazil nut

Expression

Yeast

Promoter

AOX1

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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SARS-CoV-2_N_deltaNTD

Plasmid

#157869

Purpose

express MBP tagged SARS-CoV-2 Nucleocapsid protein without the N terminal domain

Depositor

Nicolas Fawzi

Article

Perdikari et al EMBO J. 2020 Nov 17:e106478. doi: 10.152

Insert

N (N SARS-CoV-2)

Tags

MBP

Expression

Bacterial

Mutation

deltaNTD

Available Since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pSHS306 - Bacterial expression plasmid for SpCas9, HNH FRET variant

Plasmid

#87350

Purpose

S867C/S355C in cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid, HNH-1 FRET construct

Depositor

Jennifer Doudna

Article

Sternberg et al Nature. 2015 Nov 5;527(7576):110-3. doi:

Insert

Cas9

Tags

His-MBP

Expression

Bacterial

Mutation

C80S/S355C/C574S/S867C

Promoter

T7

Available Since

March 22, 2017

Availability

Academic Institutions and Nonprofits only

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JH04

Plasmid

#73513

Purpose

mRuby2_PH pCFJ150 - contains C. elegans codon optimized mRuby2

Depositor

Bob Goldstein

Article

Heppert et al Mol Biol Cell. 2016 Nov 7;27(22):3385-33

Insert

pmex-5::mRuby2::PH::tbb-2

Tags

mRuby2

Promoter

mex-5

Available Since

Feb. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pFPV25.1_dGFPmut3-AAV

Plasmid

#187377

Purpose

rpsM promoter driving the expression of destabilized GFPmut3 (containing a AAV C-terminal tail)

Depositor

Roi Avraham

Article

Ciolli Mattioli et al Curr Biol. 2023 Nov 20;33(22):4880-4892.

Insert

dGFPmut3_AAV

Tags

AAV

Expression

Bacterial

Mutation

C-terminal AAV tail

Available Since

Aug. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-fCR-GFP

Plasmid

#22911

Depositor

Edward Callaway

Article

Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

Insert

fugu calretinin promoter driving GFP

Use

AAV

Expression

Mammalian

Available Since

May 25, 2010

Availability

Academic Institutions and Nonprofits only
pMTP140 (pBAD322G_TniQ-Cascade(Tn6900))

Plasmid

#164262

Purpose

Vector expressing the TniQ, Cas8/5, Cas7, and Cas6 proteins from the Tn6900 element with an arabinose promoter.

Depositor

Joseph Peters

Article

Petassi et al Cell. 2020 Nov 30. pii: S0092-8674(20)31

Insert

Codon-optimized tniQ-cas8/5-cas7-cas6 operon from Tn6900 from A. salmonicida S44 pS44-1

Expression

Bacterial

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pUC19-U6-AksgRNA

Plasmid

#121955

Purpose

Mammalian expression, Genome editing, gRNA scaffold

Depositor

Wei Li

Article

Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

Insert

AkCas12b single chimeric gRNA

Expression

Mammalian

Available Since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pEN_TT 3xFLAG Luciferase

Plasmid

#98391

Purpose

Gateway entry vector for inducible expression of Luciferase with N-terminal 3xFLAG tag

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

Luciferase

Use

Entry vector for gateway cloning

Tags

3xFLAG

Promoter

TRE tight and TRE tight promoter

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-AkCas12b-2AeGFP

Plasmid

#121946

Purpose

Mammalian expression, Genome editing

Depositor

Wei Li

Article

Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

Insert

AkCas12b

Tags

3xHA tag

Expression

Mammalian

Available Since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-nDsRedFL-miR124

Plasmid

#177806

Purpose

The pre-miR-124 sequence has been inserted into the EcoRV of pCAG-nDsRedFL-intron.

Depositor

Rikako Sanuki

Article

Suzuki et al Genes Cells. 2020 Nov;25(11):741-752. do

Insert

miR-124

Expression

Mammalian

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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mc2 blast

Plasmid

#206217

Purpose

For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

Depositor

Simon Conn, Brett Stringer

Article

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available Since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pSCT-GAL93-PSPC1

Plasmid

#46321

Depositor

Steven Brown

Article

Kowalska et al Mol Cell Biol. 2012 Nov;32(22):4585-94.

Insert

PSPC1 (PSPC1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

July 11, 2013

Availability

Academic Institutions and Nonprofits only
pCRISPaint-T2A-Nluc-3xP3-RFP

Plasmid

#127563

Purpose

CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Nluc-SV40 and 3xP3-RFP visible eye marker.

Depositor

Norbert Perrimon

Article

Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

Insert

Nluc

Use

CRISPR

Expression

Insect

Available Since

Aug. 13, 2019

Availability

Academic Institutions and Nonprofits only

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SARS-CoV-2_N_deltaNIDR

Plasmid

#157868

Purpose

express MBP tagged SARS-CoV-2 Nucleocapsid protein without the N-terminal IDR

Depositor

Nicolas Fawzi

Article

Perdikari et al EMBO J. 2020 Nov 17:e106478. doi: 10.152

Insert

N (N SARS-CoV-2)

Tags

MBP

Expression

Bacterial

Mutation

deltaNIDR

Available Since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-Nd1

Plasmid

#191523

Purpose

Expression of mitochondrial v8(HS1) ZF-DdCBE pair in mammalian cells (LB510-Nd1+RB54-Nd1); AAV packaging vector

Depositor

David Liu

Article

Willis et al Nat Commun. 2022 Nov 23;13(1):7204. doi:

Insert

v8(HS1) ZF-DdCBE pair LB510-Nd1+RB54-Nd1 AGKS

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pYFAC-riboB

Plasmid

#168975

Purpose

Filamentous fungi vector with AMA1; 2µ replicon; URA3; AmpR ; riboB

Depositor

Yit Heng Chooi

Article

Hu et al Chem Sci. 2018 Nov 22;10(5):1457-1465. d

Type

Empty backbone

Use

Synthetic Biology; Expression in aspergillus nidu…

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pUC19-U6-AasgRNA

Plasmid

#121954

Purpose

Mammalian expression, Genome editing, gRNA scaffold

Depositor

Wei Li

Article

Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

Insert

AaCas12b single chimeric gRNA

Expression

Mammalian

Available Since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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CH00X

Plasmid

#73517

Purpose

TagRFP-T_PH pCFJ150 - contains C. elegans codon optimized TagRFP-T

Depositor

Bob Goldstein

Article

Heppert et al Mol Biol Cell. 2016 Nov 7;27(22):3385-33

Insert

pmex-5::TagRFP-T::PH::tbb-2

Tags

TagRFP-T

Promoter

mes-5

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pT7-agrBD-I

Plasmid

#53439

Purpose

T7-dependent expression of the type-I agrB and agrD genes of S. aureus RN4220

Depositor

Cynthia Collins

Article

Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003

Insert

agrBD locus

Use

Synthetic Biology

Tags

V5 epitope, 6x HIS (not expressed on agrB or agrD…

Expression

Bacterial

Mutation

none

Promoter

T7-lac

Available Since

June 3, 2014

Availability

Academic Institutions and Nonprofits only

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