We narrowed to 11,231 results for: SHA

1. 6xHis(TEV)-Drp1-StrepII

   Plasmid

   #174422

   Purpose

   Expresses human Drp1 Isoform 3 in bacteria

   Depositor

   Thomas Pucadyil

   Article

   Kamerkar et al Nat Commun. 2018 Dec 7;9(1):5239. doi: 1

   Insert

   Drp1
   

   Use

   Tags

   6xHis(TEV) and StrepII

   Expression

   Bacterial

   Mutation

   None

   Promoter

   T7

   Available since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. VPR_dCas9

   Plasmid

   #134601

   Purpose

   Express CHO codon-optimized dCas9 fused to VPR for CRISPR activation. 2A linked ZsGreen1 marker

   Depositor

   Lasse Ebdrup Pedersen

   Article

   Karottki et al Biotechnol Bioeng. 2020 Feb;117(2):593-5

   Insert

   VPR-dCas9-2A-ZsGreen-DR
   

   Use

   Tags

   VPR and ZsGreen1-DR

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. ACPPiso1-V5

   Plasmid

   #113052

   Purpose

   Expresses human prostatic acid phosphatase (isoform 1) in mammalian cells

   Depositor

   Federico Sesti

   Article

   Cotella et al FASEB J. 2013 Apr;27(4):1381-93. doi: 10

   Insert

   prostatic acid phosphatase isoform 1 (ACP3 Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. qTAG-KO-PGK-Puro

   Plasmid

   #227264

   Purpose

   Empty selectable Knock-Out donor cassette. Integrate homology arms to target the N-terminus insertion of a PGK-Puro cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. ACPPiso2-V5

   Plasmid

   #113053

   Purpose

   Expresses human prostatic acid phosphatase (isoform 2) in mammalian cells

   Depositor

   Federico Sesti

   Article

   Cotella et al FASEB J. 2013 Apr;27(4):1381-93. doi: 10

   Insert

   prostatic acid phosphatase isoform 2 (ACP3 Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. 6xHis(TEV)-Drp1(K38A)-StrepII

   Plasmid

   #174423

   Purpose

   Expresses human Drp1 Isoform 3 with K38A mutation in bacteria

   Depositor

   Thomas Pucadyil

   Article

   Kamerkar et al Nat Commun. 2018 Dec 7;9(1):5239. doi: 1

   Insert

   Drp1
   

   Use

   Tags

   6xHis(TEV) and StrepII

   Expression

   Bacterial

   Mutation

   K38A

   Promoter

   T7

   Available since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. Flag pLJM1 Rap2A

   Plasmid

   #19311

   Depositor

   David Sabatini

   Article

   Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

   Insert

   RAP2A, member of RAS oncogene family (RAP2A Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 15, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSicoR PGK puro

   Plasmid

   #12084

   Purpose

   Cre-regulated lentiviral shRNA vector. Cre addition causes both puromycin and shRNA to be recombined out of the construct, turning OFF shRNA expression.

   Depositor

   Tyler Jacks

   Article

   Ventura et al Proc Natl Acad Sci U S A. 2004 Jul 13. 1

   Type

   Empty backbone

   Use

   Cre/Lox, Lentiviral, and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 3, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFA6a-GST-kanMX6

   Plasmid

   #39293

   Depositor

   John Pringle, Jurg Bahler

   Article

   Bahler et al Yeast. 1998 Jul;14(10):943-51.

   Insert

   KanR
   

   Use

   Yeast genomic targeting

   Tags

   A. gossypii translation elongation factor 1a gene…

   Expression

   Mutation

   Promoter

   Available since

   Aug. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAJM.1642

    Plasmid

    #108535

    Purpose

    CinRAM + PCin-YFP Reporter. OHC14 sensor for use in non-Marionette strain.

    Depositor

    Christopher Voigt

    Article

    Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

    Insert

    CinRAM + PCin-YFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    CinR: S72C V80I G114D G115D R137H K220R A224T *24…

    Promoter

    Available since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAJM.690

    Plasmid

    #108533

    Purpose

    PcaUAM + P3B5B-YFP Reporter. DHBA sensor for use in non-Marionette strain.

    Depositor

    Christopher Voigt

    Article

    Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

    Insert

    PcaUAM + P3B5B-YFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    PcaU: K14N A165T N201H

    Promoter

    Available since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAJM.884

    Plasmid

    #108536

    Purpose

    AcuRAM + PAcu-YFP Reporter. Acr sensor for use in non-Marionette strain.

    Depositor

    Christopher Voigt

    Article

    Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

    Insert

    AcuRAM + PAcu-YFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    AcuR: E80K R154H L202M

    Promoter

    Available since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLKO.1 RagC shRNA #1

    Plasmid

    #26629

    Depositor

    David Sabatini

    Article

    Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

    Insert

    RagC shRNA (RRAGC Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

14. pFA6a-GST-His3MX6

    Plasmid

    #41604

    Depositor

    John Pringle

    Article

    Longtine et al Yeast. 1998 Jul;14(10):953-61.

    Insert

    His3
    

    Use

    Yeast genomic targeting

    Tags

    A. gossypii translation elongation factor 1a gene…

    Expression

    Mutation

    Promoter

    Available since

    Dec. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTE102

    Plasmid

    #59397

    Purpose

    MethyloBrick expression vector, mxaF promoter; TcR

    Depositor

    Tobias Erb

    Article

    Schada von Borzyskowski et al ACS Synth Biol. 2014 Aug 8.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PmxaF

    Available since

    Dec. 30, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFA6a-GFP(S65T)-kanMX6

    Plasmid

    #39292

    Depositor

    John Pringle, Jurg Bahler

    Article

    Bahler et al Yeast. 1998 Jul;14(10):943-51.

    Insert

    KanR
    

    Use

    Yeast genomic targeting

    Tags

    A. gossypii translation elongation factor 1a gene…

    Expression

    Mutation

    Promoter

    Available since

    Aug. 14, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLKO.1 RagC shRNA #2

    Plasmid

    #26630

    Depositor

    David Sabatini

    Article

    Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

    Insert

    RagC shRNA (RRAGC Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

18. pSLC-246

    Plasmid

    #73190

    Purpose

    Template plasmid which encodes kanamycin resistance gene for positive selection and toxin gene (tse2) under the control of rhamnose induceable promoter (PrhaB) for negative selection.

    Depositor

    Swaine Chen

    Article

    Khetrapal et al Nucleic Acids Res. 2015 Jul 27;43(13):e8

    Insert

    PrhaB-tse2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PrhaB

    Available since

    July 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHiUGE GS-gRNA (no Cre)

    Plasmid

    #200370

    Purpose

    Backbone plasmid for cloning HiUGE GS-gRNA (without Cre)

    Depositor

    Scott Soderling

    Article

    Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NA

    Promoter

    Available since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pPP537

    Plasmid

    #158803

    Purpose

    pRSF-Duet1 derived plasmid containing C-terminally 2xSV40 NLS and hexa-histidine tagged CasΦ-2 in MCS1 for expression in E. coli and protein purification

    Depositor

    Jennifer Doudna

    Article

    Pausch et al Science. 2020 Jul 17;369(6501):333-337.

    Insert

    CasPhi-2
    

    Use

    Tags

    2xSV40NLS-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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