Addgene

We narrowed to 21,417 results for: ESC;

Showing: 7711 - 7740 of 21417 results

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pTn7-SCOUT14.Y

Plasmid

#201009

Purpose

pLv1-pUC18R6KT-miniTn7-aad9-sYFP2, SpR,StR, ApR

Depositor

Philip Poole

Article

Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

Insert

sYFP2

Use

Tags

Expression

Bacterial

Mutation

Promoter

J23100

Available since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTn7-SCOUT22.YB

Plasmid

#201019

Purpose

pLv1-pUC18R6KT-miniTn7-Km-sYFP2-TagBFP, KmR, ApR

Depositor

Philip Poole

Article

Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

Inserts

sYFP2
TagBFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

J23104

Available since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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lentiCRISPRv2-Blast-mU6

Plasmid

#206806

Purpose

pLentiCRISPRv2 variant with mU6 promoter driving the sgRNA, and a Blasticidin resistance cassette

Depositor

Jolanda Van Leeuwen

Article

Unlu et al Genome Med. 2023 Oct 12;15(1):78. doi: 1

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mU6

Available since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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CH-GECO2.1

Plasmid

#52099

Purpose

mCherry-based Calcium indicators

Depositor

Robert Campbell

Article

Carlson et al Protein Eng Des Sel. 2013 Dec;26(12):763

Insert

CH-GECO2.1

Use

Tags

6 His tag

Expression

Bacterial

Mutation

Promoter

pBAD promotor

Available since

April 14, 2014

Availability

Academic Institutions and Nonprofits only
GFP E17TAG/Q157TAG

Plasmid

#68299

Purpose

E17/Q157 TAG GFP reporter

Depositor

Jesse Rinehart

Article

Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

Insert

GFP E17TAG/Q157TAG

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pET28a-MinE-His

Plasmid

#133623

Purpose

Expression vector for the Escherichia coli MinE with an C-terminal fusion of a His-tag for purification.

Depositor

Petra Schwille

Article

Glock et al ACS Synth Biol. 2018 Dec 20. doi: 10.102

Insert

MinE-His (minE E. coli)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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mTagBFP2-Vinculin-23

Plasmid

#55334

Purpose

Localization: Focal Adhesions, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Article

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Insert

Vinculin (VCL Human)

Use

Tags

mTagBFP2

Expression

Mammalian

Mutation

V234L in Vinculin.

Promoter

CMV

Available since

Oct. 22, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pAJM.944

Plasmid

#108523

Purpose

PCin-YFP Reporter. OHC14 sensor for use in Marionette strain.

Depositor

Christopher Voigt

Article

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

PCin-YFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yoPSmOrange-Kan

Plasmid

#44951

Purpose

Yeast tagging vector to create gene fusion with yeast optimized photoconvertible mOrange with Kan selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoPSmOrange

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 16, 2013

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yomKeima-CaURA3

Plasmid

#44885

Purpose

Yeast tagging vector to create gene fusion with yeast optimized mKeima with CaUra3 selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yomKeima

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 16, 2013

Availability

Academic Institutions and Nonprofits only

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pFA6-mTFP1-KanMX6

Plasmid

#69851

Purpose

C-terminal tagging with mTFP1 using KanMX6 as a selection marker in S. cerevisiae.

Depositor

Liza Pon

Article

Higuchi-Sanabria et al PLoS One. 2016 Jan 4;11(1):e0146120. doi

Insert

mTFP1

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 16, 2018

Availability

Academic Institutions and Nonprofits only

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pBabe hygro MTH1

Plasmid

#21296

Depositor

Bob Weinberg

Article

Rai et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

Insert

MTH1 (NUDT1 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 29, 2009

Availability

Academic Institutions and Nonprofits only
pAE03

Plasmid

#61044

Purpose

To construct C-terminal GFP fusions, single crossover integration vector

Depositor

Jan-Willem Veening

Article

Eberhardt et al Mol Microbiol. 2009 Oct;74(2):395-408. d

Type

Empty backbone

Use

Synthetic Biology

Tags

GFP+

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pBAD/HisD-TagRFP675

Plasmid

#44274

Depositor

Vladislav Verkhusha

Article

Piatkevich et al Sci Rep. 2013;3:1847. doi: 10.1038/srep0

Insert

TagRFP675

Use

Tags

Expression

Bacterial

Mutation

M41Q/F80W/S143N/L147M/S158N/D159Y/N173S

Promoter

pBAD

Available since

May 16, 2013

Availability

Academic Institutions and Nonprofits only

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pMito.iGABASnFR.F102G.mRuby3

Plasmid

#112180

Purpose

GABA Sensor

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

Insert

iGABASnFR.F102G

Use

Tags

mRuby3

Expression

Mammalian

Mutation

F102G

Promoter

Available since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pAJM.713

Plasmid

#108514

Purpose

PLuxB-YFP Reporter. OC6 sensor for use in Marionette strain.

Depositor

Christopher Voigt

Article

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

PLuxB-YFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 30, 2018

Availability

Academic Institutions and Nonprofits only

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pBSV2G_PflaB-iRFPBb

Plasmid

#118237

Purpose

Gentamicin-resistant E.coli/B. burgdorferi shuttle vector; Encodes flagellin promoter driven iRFP

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

Insert

flagellin promoter driven iRFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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pSB_init-Sb44

Plasmid

#159421

Purpose

Expression of the anti-TGP (thermostable GFP) synthetic nanobody Sb44

Depositor

Dianfan Li

Article

Cai et al Commun Biol. 2020 Dec 10;3(1):753. doi:

Insert

Sb44

Use

Tags

c-Myc and poly-His

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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mCerulean3-Talin-18

Plasmid

#55447

Purpose

Localization: Focal Adhesions, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

Talin

Use

Tags

mCerulean3

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 10, 2014

Availability

Academic Institutions and Nonprofits only

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myr-palm_mCherry-mEGFP

Plasmid

#127815

Purpose

Hetero-dimer mCherry-mEGFP expression vector with plasma membrane localization signal

Depositor

Salvatore Chiantia

Article

Dunsing et al Sci Rep. 2018 Jul 13;8(1):10634. doi: 10

Insert

myristoylated-palmitoylated-mCherry-mEGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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Gq-sensor[mTq2-sREACh]

Plasmid

#98835

Purpose

A FRET sensor for Gq activation, encoded on a single plasmid, for FLIM

Depositor

Dorus Gadella

Article

Mastop et al Sci Rep. 2017 Sep 20;7(1):11999. doi: 10

Insert

mTq2-sREACh

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pMUT2-pCaf-mCherry

Plasmid

#192860

Purpose

pMUT2 based vector for sensing caffeine in E. coli Nissle 1917

Depositor

Morten Sommer

Article

Vaaben et al ACS Synth Biol. 2022 Dec 16;11(12):4184-

Insert

Nanobody-CadC fusion protein and cognate promoter inducible by caffeine

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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CytERM-dTomato

Plasmid

#98834

Purpose

In vivo visualization of the ER (can be used for colocalization studies and the OSER assay)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

CytERM

Use

Tags

dTomato

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pUC19/N-mChe

Plasmid

#183159

Purpose

Plant transient expression vector containing mCherry tag. Unique nucleotide sequences Uc and Ud for cloning at N terminus of mCherry.

Depositor

Qinlong Zhu, Yao-Guang Liu

Article

Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

April 26, 2022

Availability

Academic Institutions and Nonprofits only

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pMito-iRFP713

Plasmid

#45465

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

Insert

iRFP713

Use

Tags

human cytochrome C oxidase subunit VIII

Expression

Mammalian

Mutation

T2A, S13L, A92T, V104T, V114I, E161K, Y193K, F198…

Promoter

CMV

Available since

June 17, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3-GR-ICUE2

Plasmid

#173017

Purpose

Improved green-red FRET based cAMP indicator.

Depositor

Jin Zhang

Article

Mo et al Nat Commun. 2020 Apr 15;11(1):1848. doi:

Insert

GR-ICUE2

Use

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pGas-miRFP670nano

Plasmid

#127441

Purpose

G protein alpha-s internally tagged with miRFP670nano

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

Insert

G protein alpha-s-miRFP670nano

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 17, 2019

Availability

Academic Institutions and Nonprofits only

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pNB648

Plasmid

#60140

Purpose

NanoLuc luciferase with a nuclear localization signal (NLS) driven by MET17 promoter.

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

NLuc

Use

Tags

Nuclear localization signal (NLS)

Expression

Yeast

Mutation

Promoter

MET17pr

Available since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

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SPDK3181

Plasmid

#97403

Purpose

binary construct of ER-targeted sfCherry1-10 for plant expression

Depositor

Savithramma Dinesh-Kumar

Article

Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

Insert

ER-sfCherry1-10

Use

Tags

ER target

Expression

Plant

Mutation

Promoter

Available since

Sept. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pAJM.718

Plasmid

#108519

Purpose

PBetI-YFP Reporter. Cho sensor for use in Marionette strain.

Depositor

Christopher Voigt

Article

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

PBetI-YFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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