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pTHSSe_50

Plasmid

#109248

Purpose

incW BbsI cloning vector

Depositor

Christopher Voigt

Article

Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

Insert

sfGFP

Expression

Bacterial

Promoter

PJ23105

Available Since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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pSDM177

Plasmid

#194476

Purpose

pHERD30T-crRNA(Lse)_V3

Depositor

Joseph Bondy-Denomy

Article

Guan et al Nat Microbiol. 2022 Oct 31. pii: 10.1038

Insert

crRNA(Lse)

Expression

Bacterial

Available Since

Sept. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pAW019-2

Plasmid

#85614

Purpose

Inducible dCas9 integration vector for Bacillus subtilis

Depositor

Perry Chou

Article

Westbrook et al Appl Environ Microbiol. 2016 Jun 3. pii:

Insert

dcas9

Expression

Bacterial

Promoter

PxylA (B. megaterium)

Available Since

Jan. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pRSET-mEosEM

Plasmid

#132708

Purpose

application in Correlative light-electron microscopy

Depositor

Pingyong Xu

Article

Fu et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

Insert

mEosEM

Expression

Bacterial

Available Since

Oct. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pDisplay-BirA-ER

Plasmid

#20856

Depositor

Alice Ting

Article

Howarth et al Nat Methods. 2008 May . 5(5):397-9.

Insert

BirA-ER

Expression

Mammalian

Mutation

C-terminal ER targeting sequence (KDEL)

Available Since

May 7, 2009

Availability

Academic Institutions and Nonprofits only

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Lac-I-SceI-Tet

Plasmid

#17655

Depositor

Tom Misteli

Article

Soutoglou et al Nat Cell Biol. 2007 Jun . 9(6):675-82.

Insert

lac I-SceI tet

Expression

Bacterial

Available Since

May 30, 2008

Availability

Academic Institutions and Nonprofits only
pCJ162

Plasmid

#175055

Purpose

lox66-72 flanked gentamycin resistance cassette integrates into aquI integration site

Depositor

David Nielsen

Article

Jones et al ACS Synth Biol. 2021 Sep 17;10(9):2371-2

Insert

lox66::gmR::lox71

Use

Unspecified

Promoter

n/a

Available Since

Jan. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pPB_EF1a_Gag-EIF4E

Plasmid

#212657

Purpose

piggyBac vector to express an MLV Gag-EIF4E fusion protein

Depositor

Paul Blainey

Article

Najia et al bioRxiv 2024.10.01.616098

Insert

MLV Gag (UniProt ID: P03332) (gag MoMLV)

Use

Piggybac

Tags

EIF4E

Expression

Mammalian

Promoter

EF1a

Available Since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pLV_CAG_Gag-EIF4E

Plasmid

#212635

Purpose

Lentivirus vector to express an MLV Gag-EIF4E fusion protein

Depositor

Paul Blainey

Article

Najia et al bioRxiv 2024.10.01.616098

Insert

MLV Gag (UniProt ID: P03332) (gag MoMLV)

Use

Lentiviral

Tags

EIF4E

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pYPQ146

Plasmid

#69298

Purpose

Golden Gate recipient and Gateway entry vector; assembly of 6 gRNAs

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available Since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pPTn2P-PTK

Plasmid

#85604

Purpose

Passport transposon with a puromycin-thymidine kinase fusion driven by a PGK promoter.

Depositor

Karl Clark, Scott Fahrenkrug

Article

Clark et al BMC Biotechnol. 2007 Jul 17;7:42.

Insert

Passport transposon with a puromycin-thymidine kinase fusion

Expression

Mammalian

Promoter

PGK

Available Since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pMYT095

Plasmid

#198866

Purpose

CRISPR/Cas9 Vector with URA3 selectable marker to be used in the Ellis MYT system

Depositor

Tom Ellis

Article

Shaw et al ACS Synth Biol. 2023 Nov 6. doi: 10.1021

Type

Empty backbone

Use

Synthetic Biology

Available Since

April 12, 2023

Availability

Academic Institutions and Nonprofits only

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pMYT096

Plasmid

#198867

Purpose

Transient CRISPR/Cas9 Vector without a selectable marker to be used in the Ellis MYT system

Depositor

Tom Ellis

Article

Shaw et al ACS Synth Biol. 2023 Nov 6. doi: 10.1021

Type

Empty backbone

Use

Synthetic Biology

Available Since

April 12, 2023

Availability

Academic Institutions and Nonprofits only

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pYPQ141A

Plasmid

#69290

Purpose

Gateway entry vector; single gRNA under AtU6 promoter

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available Since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

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MyD116.PFLAG.CMV2

Plasmid

#21834

Depositor

David Ron

Article

Novoa et al J Cell Biol. 2001 May 28. 153(5):1011-22

Insert

GADD34 (Ppp1r15a Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Sept. 14, 2009

Availability

Academic Institutions and Nonprofits only

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pHR_D17_hrGFP

Plasmid

#84616

Purpose

HR donor to integrate UAS1B8TEF(136)-hrGFP-CYC1t into D17 locus

Depositor

Ian Wheeldon

Article

Schwartz et al ACS Synth Biol. 2016 Dec 19.

Insert

hrGFP

Use

Synthetic Biology

Expression

Yeast

Promoter

UAS1B8-TEF(136)

Available Since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pYPQ144

Plasmid

#69296

Purpose

Golden Gate recipient and Gateway entry vector; assembly of 4 gRNAs

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available Since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pYD1-mSA

Plasmid

#39865

Depositor

Sheldon Park

Article

Lim et al Biotechnol Bioeng. 2013 Jan;110(1):57-67

Insert

monomeric streptavidin

Tags

Aga2, FLAG, and HA

Expression

Yeast

Available Since

March 15, 2013

Availability

Academic Institutions and Nonprofits only

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pHR_MFE1_hrGFP

Plasmid

#84617

Purpose

HR donor to integrate UAS1B8TEF(136)-hrGFP-CYC1t into MFE1 locus

Depositor

Ian Wheeldon

Article

Schwartz et al ACS Synth Biol. 2016 Dec 19.

Insert

hrGFP

Use

Synthetic Biology

Expression

Yeast

Promoter

UAS1B8-TEF(136)

Available Since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pCMK191

Plasmid

#226658

Purpose

Broad host range WVO1 vector that replicates in Synechococcus sp. PCC 7002, GentR, contains HIP1 site

Depositor

David Nielsen

Article

Kamoku et al Synthetic Biology and Engineering 2024,

Type

Empty backbone

Use

Unspecified

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pCEV-G1-Km

Plasmid

#46813

Purpose

TEF1 and PGK1 promoter controlled expression cassettes with G418 resistance for yeast transformation

Depositor

Lars Nielsen, Claudia Vickers

Article

Vickers et al Microb Cell Fact. 2013 Oct 25;12(1):96.

Type

Empty backbone

Tags

FLAG and c-myc

Expression

Yeast

Promoter

TEF1 and PGK1

Available Since

Oct. 11, 2013

Availability

Academic Institutions and Nonprofits only

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EGFP-fMCP

Plasmid

#238908

Purpose

Contains EGFP-fMCP fluorogenic protein

Depositor

Jian-Hui Jiang

Article

Zhou et al Angew Chem Int Ed Engl. 2025 Apr 10:e202

Insert

EGFP-fMCP

Expression

Mammalian

Promoter

miniCMV (GGTAGGCGTGTACGGTGGGAGGCCTATATAAGCAGAGCT)

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_Lifeact-EGFP

Plasmid

#67303

Purpose

Fluorescent indicator for F-actin

Depositor

Moritoshi Sato

Article

Kawano et al Nat Commun. 2015 Feb 24;6:6256. doi: 10.

Insert

Lifeact-EGFP

Expression

Mammalian

Promoter

CMV

Available Since

July 9, 2015

Availability

Academic Institutions and Nonprofits only

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pLPC myc hPOT1

Plasmid

#12387

Depositor

Titia de Lange

Article

Loayza et al Nature. 2003 Jun 26. 423(6943):1013-8.

Insert

Protection of Telomeres 1 (POT1 Human)

Use

Retroviral

Tags

Myc

Available Since

Oct. 20, 2006

Availability

Academic Institutions and Nonprofits only

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pHR_CMV_Melt-30-mCherry

Plasmid

#234061

Purpose

Encodes a Melt variant with a switching temperature of 30°C

Depositor

Lukasz Bugaj

Article

Benman et al Nat Methods. 2025 Jan 23. doi: 10.1038/s

Insert

Melt-30-mCherry

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pHR_XPR2_hrGFP

Plasmid

#84614

Purpose

HR donor to integrate UAS1B8TEF(136)-hrGFP-CYC1t into XPR2 locus

Depositor

Ian Wheeldon

Article

Schwartz et al ACS Synth Biol. 2016 Dec 19.

Insert

hrGFP

Use

Synthetic Biology

Expression

Yeast

Promoter

UAS1B8-TEF(136)

Available Since

Nov. 7, 2016

Availability

Academic Institutions and Nonprofits only

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haA1.pBABEpu

Plasmid

#21813

Depositor

David Ron

Article

Novoa et al J Cell Biol. 2001 May 28. 153(5):1011-22

Insert

GADD34 C-term

Use

Retroviral

Expression

Mammalian

Available Since

Sept. 10, 2009

Availability

Academic Institutions and Nonprofits only

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pMT mCherry-CaaX

Plasmid

#126669

Purpose

Inducible expression of mCherry-CaaX in S2 cells

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

mCherry-CaaX

Expression

Insect

Available Since

Jan. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pHR_A08_hrGFP

Plasmid

#84615

Purpose

HR donor to integrate UAS1B8TEF(136)-hrGFP-CYC1t into A08 locus

Depositor

Ian Wheeldon

Article

Schwartz et al ACS Synth Biol. 2016 Dec 19.

Insert

hrGFP

Use

Synthetic Biology

Expression

Yeast

Promoter

UAS1B8-TEF(136)

Available Since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pKmK.P2

Plasmid

#125035

Purpose

Storage plasmid for K.marxianus promoter; type 2 part

Depositor

John Morrissey

Article

Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

Insert

PDC1 promoter

Use

Synthetic Biology

Expression

Yeast

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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