We narrowed to 22,195 results for: his

1. pQE60 Q58L wgMDH

   Plasmid

   #204246

   Purpose

   Bacterial expression of Q58L wgMDH with IPTG induction - Subunit interface mutant

   Depositor

   Malate dehydrogenase CUREs Community (MCC)

   Article

   Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

   Insert

   MDHG_CITLA
   

   Use

   Tags

   6X His

   Expression

   Bacterial

   Mutation

   Q58L

   Promoter

   T5

   Available Since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET28a_Pro-IL-33

   Plasmid

   #214342

   Purpose

   Protein overexpression in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

   Insert

   IL33 (IL33 Human)
   

   Use

   Tags

   His-TEV and Myc

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET28a_Pro-IL-1β

   Plasmid

   #214340

   Purpose

   Protein overexpression in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

   Insert

   IL1B (IL1B Human)
   

   Use

   Tags

   His-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. RET-V5

   Plasmid

   #139627

   Purpose

   V5-His-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Yadav et al iScience. 2020 Feb 21;23(2):100871. doi:

   Insert

   RET (RET Human)
   

   Use

   Tags

   3xV5-6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. STAT3(Y705F)-TAL-Luc

   Plasmid

   #46933

   Purpose

   Luciferase Reporter for mutant STAT3

   Depositor

   Afshin Dowlati

   Article

   Dabir et al Mol Cancer Res. 2009 Nov;7(11):1854-60.

   Insert

   STAT3(Y705F) (STAT3 Human)
   

   Use

   Luciferase

   Tags

   FLAG

   Expression

   Mutation

   Tyrosine 705 to Phenylalanine

   Promoter

   TA Luciferase

   Available Since

   Nov. 7, 2013

   Availability

   Academic Institutions and Nonprofits only

6. HP35 tension sensor

   Plasmid

   #101250

   Purpose

   Expresses the HP35-based tension sensor module. The biosensor allows force measurements at 6-8 pN.

   Depositor

   Carsten Grashoff

   Article

   Austen et al Nat Cell Biol. 2015 Dec;17(12):1597-606.

   Insert

   YPet(short)-HP35-mCherry
   

   Use

   Retroviral

   Tags

   His-tag and cysteine

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Human J-chain

   Plasmid

   #158215

   Purpose

   Express the human J-chain

   Depositor

   Junyu Xiao

   Article

   Li et al Science. 2020 Feb 28;367(6481):1014-1017

   Insert

   JCHAIN (JCHAIN Human)
   

   Use

   Tags

   8×his

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. bNS2B47NS3

   Plasmid

   #155320

   Purpose

   co-expression of DENV4 47 peptides from NS2B cofactor and NS3 full length

   Depositor

   Dahai Luo

   Article

   Phoo et al Antiviral Res. 2020 Oct;182:104900. doi:

   Insert

   Co expression of DENV4 NS3 full length protein with 47 peptides from NS2B cofactor
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available Since

   Nov. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET_RGG-GFP-RGG

   Plasmid

   #124948

   Purpose

   Expression of RGG-GFP-RGG

   Depositor

   Daniel Hammer, Benjamin Schuster, Matthew Good

   Article

   Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

   Insert

   RGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
   

   Use

   Tags

   EGFP and His

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   May 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pQStrep2-CRYM

    Plasmid

    #31293

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    crystallin, mu (CRYM Human)
    

    Use

    Tags

    His and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAB46 (CK2beta)

    Plasmid

    #27085

    Depositor

    David Litchfield

    Article

    Turowec et al Methods Enzymol. 2010;484:471-93.

    Insert

    CK2beta (CSNK2B Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. tUI

    Plasmid

    #122661

    Purpose

    Express tUI protein in E. coli

    Depositor

    Robert Cohen

    Article

    Choi et al Nat Methods. 2019 Jul 15. pii: 10.1038/s

    Insert

    tUI (UBQLN1, USP5 Human)
    

    Use

    Tags

    His-tag

    Expression

    Mutation

    Promoter

    T7 promoter

    Available Since

    July 17, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pcDNAFLAG-ATM-kd

    Plasmid

    #43908

    Depositor

    Stephen Elledge

    Article

    Cortez et al Science. 1999 Nov 5. 286(5442):1162-6.

    Insert

    ataxia telangiectasia mutated (ATM Human)
    

    Use

    Tags

    FLAG and His

    Expression

    Mammalian

    Mutation

    kinase dead (D2870A and N2875K)

    Promoter

    Available Since

    Aug. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pJSC111 - Bacterial expression plasmid for SpCas9-HF1 variant

    Plasmid

    #101209

    Purpose

    Bacterial expression plasmid for SpCas9-HF1 variant

    Depositor

    Jennifer Doudna, Keith Joung

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant N497A/R661A/Q695A/Q926A
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    N497A, R661A, Q695A and Q926A

    Promoter

    T7

    Available Since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET 28 full-length human PARP1

    Plasmid

    #192635

    Purpose

    Expresses human PARP1 with an N-terminal His-tag

    Depositor

    John Pascal

    Article

    Langelier et al J Biol Chem. 2008 Feb 15;283(7):4105-14.

    Insert

    Human PARP1 (PARP1 Human)
    

    Use

    Tags

    histidine-tag

    Expression

    Bacterial

    Mutation

    Polymorphism A762

    Promoter

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET_MBP-RGG-GFP-RGG

    Plasmid

    #124949

    Purpose

    Expression of MBP-RGG-GFP-RGG

    Depositor

    Daniel Hammer, Benjamin Schuster, Matthew Good

    Article

    Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

    Insert

    MBP-RGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
    

    Use

    Tags

    EGFP, His, and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJSC114 - Bacterial expression plasmid for SpCas9 eSpCas9(1.1) variant

    Plasmid

    #101215

    Purpose

    Bacterial expression plasmid for SpCas9 eSpCas9(1.1) variant

    Depositor

    Jennifer Doudna, Keith Joung

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant K848A/K1003A/R1060A
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    K848A, K1003A and R1060A

    Promoter

    T7

    Available Since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV Syn CaMPARI2-F391W_L398V-human-a-Tubulin (high affinity TubuTag)

    Plasmid

    #164193

    Purpose

    Freeze-frame imaging of dendritic calcium signals High calcium affinity (CaMPARI2_F391W_L398V moiety Kd=174nM)

    Depositor

    Thomas Oertner

    Article

    Perez-Alvarez et al Front Mol Neurosci. 2021 Mar 4;14:635820

    Inserts

    CamPARI2
    Human α Tubulin
    

    Use

    AAV

    Tags

    6 His tag and NES

    Expression

    Mammalian

    Mutation

    F391W-L398V

    Promoter

    Syn

    Available Since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. EGFP-hPMCA2w/b

    Plasmid

    #47586

    Depositor

    Emanuel Strehler

    Article

    Chicka et al J Biol Chem. 2003 May 16;278(20):18464-7

    Insert

    PMCA2w/b (ATP2B2 Human)
    

    Use

    Tags

    EGFP-HA-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET15b CnA CnB

    Plasmid

    #11787

    Depositor

    Anjana Rao

    Article

    Mondragon et al Biochemistry. 1997 Apr 22. 36(16):4934-4

    Insert

    CnA CnB (PPP3R1, PPP3CA Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 4, 2006

    Availability

    Academic Institutions and Nonprofits only

21. pET51b(+)-NADPH-LUCID-3 (C50 1.4 uM)

    Plasmid

    #117910

    Purpose

    Plasmid encoding the bacterial expression of the BRET semi-synthetic biosensor measuring the NADPH concentration with a C50 of 1.4 uM

    Depositor

    Kai Johnsson

    Article

    Yu et al Science. 2018 Sep 14;361(6407):1122-1126

    Insert

    SNAP_Pro30_[cpNLuc23_24]eDHFR(R44A)
    

    Use

    Tags

    His-tag and Strep-tag

    Expression

    Bacterial

    Mutation

    R44A on eDHFR

    Promoter

    T7

    Available Since

    Oct. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pABCb10-GFP

    Plasmid

    #19031

    Depositor

    Orian Shirihai

    Article

    Graf et al J Biol Chem. 2004 Oct 8. 279(41):42954-6

    Insert

    ATP-binding cassette, sub-family B (MDR/TAP), member 10 (Abcb10 Mouse)
    

    Use

    Tags

    HIS

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLEGFP-S727A-STAT3

    Plasmid

    #71446

    Purpose

    Retroviral expression of S727A-STAT3. Please note that this plasmid contains S727A-STAT3 tagged with FLAG, not GFP.

    Depositor

    George Stark

    Article

    Dasgupta et al Proc Natl Acad Sci U S A. 2015 Mar 31;11

    Insert

    STAT3 (STAT3 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mutation

    S727A

    Promoter

    CMV

    Available Since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pRSFDuet-1_VNp-mNeongreen_OmpA-mCherry

    Plasmid

    #182390

    Purpose

    Bacterial expression of Vesicle Nucleating peptide-mNeongreen and ompA-mCherry fusions

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Inserts

    VNp-mNeongreen-His
    OmpA-mCherry
    

    Use

    Tags

    Vesicle Nucleating peptide (VNp)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCherry-mNrp1

    Plasmid

    #21934

    Depositor

    Guido Serini

    Article

    Valdembri et al PLoS Biol. 2009 Jan 27. 7(1):e25.

    Insert

    neuropilin 1 (Nrp1 Mouse)
    

    Use

    Tags

    Cherry and His

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

26. pQTEV-ASF1A

    Plasmid

    #31591

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    ASF1 anti-silencing function 1 homolog A (S. cerevisiae) (ASF1A Human)
    

    Use

    Tags

    His and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET28a_Pro-IL-1α

    Plasmid

    #214341

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    IL1A (IL1A Human)
    

    Use

    Tags

    His-TEV and Myc

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. α-actinin-4-M522-Venus in pcDNA6

    Plasmid

    #190802

    Purpose

    Imaging of α-actinin-4 in live cells

    Depositor

    Vivian Tang

    Article

    Morris et al J Cell Biol. 2022 May 2;221(5). pii: 213

    Insert

    alpha-actiinin-4-M522-venus (ACTN4 Synthetic, Human)
    

    Use

    Tags

    His, myc, and venus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET_MBP-RGG-RGG

    Plasmid

    #124946

    Purpose

    Expression of MBP-RGG-RGG

    Depositor

    Daniel Hammer, Benjamin Schuster, Matthew Good

    Article

    Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

    Insert

    MBP-RGG-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
    

    Use

    Tags

    His and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. tetO-NKX2.5

    Plasmid

    #46029

    Depositor

    John Gearhart

    Article

    Addis et al J Mol Cell Cardiol. 2013 Apr 13;60C:97-1

    Insert

    NKX2.5 (NKX2-5 Human)
    

    Use

    Lentiviral

    Tags

    His and V5

    Expression

    Mammalian

    Mutation

    Promoter

    tetO

    Available Since

    July 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. SB_concave

    Plasmid

    #110102

    Purpose

    pBXPHM3 with scaffold Sybody of the concave library

    Depositor

    Markus Seeger

    Article

    Zimmermann et al Elife. 2018 May 24;7. pii: 34317. doi: 1

    Insert

    scaffold Sybody of the concave library
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    PBAD

    Available Since

    May 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pYFJ16-LplA(W37V) (for E. Coli expression)

    Plasmid

    #34838

    Depositor

    Alice Ting

    Article

    Uttamapinant et al Proc Natl Acad Sci U S A. 2010 Jun 15. 1

    Insert

    LplA W37V (lplA E. Coli)
    

    Use

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    Mutated Trp37 to Val

    Promoter

    T5 promoter

    Available Since

    May 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pFastBac_human-IL-18

    Plasmid

    #214338

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    IL18 (IL18 Human)
    

    Use

    Tags

    His-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pET28C-mCherry-53BP1LCD

    Plasmid

    #204407

    Purpose

    E. coli expression of the 53BP1 low-complexity domain (1208-1977) with an N-terminal mCherry tag

    Depositor

    Samie Jaffrey

    Article

    Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

    Insert

    53BP1(1208-1977) (TP53BP1 Human)
    

    Use

    Tags

    6x-His; mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCG-Hc∆18-AA-IL-13

    Plasmid

    #86171

    Purpose

    Plasmid encodes blinded measles virus H glycoprotein displaying IL-13

    Depositor

    Jakob Reiser

    Article

    Ou et al Hum Gene Ther Methods. 2012 Apr;23(2):13

    Insert

    Measles virus H glycoprotein fused to IL-13
    

    Use

    Tags

    6x His

    Expression

    Mammalian

    Mutation

    Y463A, R515A

    Promoter

    Available Since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. SB_convex

    Plasmid

    #110104

    Purpose

    pBXPHM3 with scaffold Sybody of the convex library

    Depositor

    Markus Seeger

    Article

    Zimmermann et al Elife. 2018 May 24;7. pii: 34317. doi: 1

    Insert

    scaffold Sybody of the convex library
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    PBAD

    Available Since

    May 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pET21b-Caspase-3(C163S)

    Plasmid

    #90088

    Purpose

    Expresses caspase 3 with C163S to prevent autocatalysis in bacterial cells

    Depositor

    Clay Clark

    Article

    Pop et al Biochemistry. 2001 Nov 27;40(47):14224-3

    Insert

    Caspase 3 (CASP3 Human)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    C163S

    Promoter

    Available Since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. SB_loop

    Plasmid

    #110103

    Purpose

    pBXPHM3 with scaffold Sybody of the loop library

    Depositor

    Markus Seeger

    Article

    Zimmermann et al Elife. 2018 May 24;7. pii: 34317. doi: 1

    Insert

    scaffold Sybody of the loop library
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    PBAD

    Available Since

    May 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pETDuet-1_VNp-mNeongreen_CydAB-mCherry

    Plasmid

    #182391

    Purpose

    Bacterial expression of Vesicle Nucleating peptide-mNeongreen and cydAB-mCherry fusions

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Inserts

    VNp-mNeongreen-His
    cydAB-mCherry
    

    Use

    Tags

    Vesicle Nucleating peptide (VNp)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. D. vulgaris Cascade/I-C (Cas5c-Cas8c-Cas7)/pHMGWA

    Plasmid

    #81185

    Purpose

    Expresses Desulfovibrio vulgaris Cascade/I-C complex subunits in E. coli with TEV-cleavable N-terminal His-MBP tag on Cas5c

    Depositor

    Jennifer Doudna

    Article

    Hochstrasser et al Mol Cell. 2016 Sep 1;63(5):840-51. doi:

    Inserts

    Cas5c
    Cas8c
    Cas7
    

    Use

    CRISPR

    Tags

    6xHis - MBP - TEV protease site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge