We narrowed to 23,191 results for: his

Showing: 10321 - 10360 of 23191 results

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NpT7-5-Erk2

Plasmid

#39230

Depositor

Melanie Cobb

Article

Robbins et al J Biol Chem. 1993 Mar 5;268(7):5097-106.

Insert

Erk2 (Mapk1 Rat)

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

July 15, 2013

Availability

Academic Institutions and Nonprofits only
pCellFree_G03 BATF3

Plasmid

#67050

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

BATF3 (BATF3 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pQE60 R196M wgMDH

Plasmid

#204273

Purpose

Bacterial expression of R196M wgMDH with IPTG induction - Active site mutant

Depositor

Malate dehydrogenase CUREs Community (MCC)

Article

Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

Insert

MDHG_CITLA

Tags

6X His

Expression

Bacterial

Mutation

R196M

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pQE60 D193E wgMDH

Plasmid

#204269

Purpose

Bacterial expression of D193E wgMDH with IPTG induction - Active site mutant

Depositor

Malate dehydrogenase CUREs Community (MCC)

Article

Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

Insert

MDHG_CITLA

Tags

6X His

Expression

Bacterial

Mutation

D193E

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 TGFB1

Plasmid

#67080

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

TGFB1 (TGFB1 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
Nano-lantern-plasma membrane/pcDNA3

Plasmid

#51975

Purpose

mammalian expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging, targeted to the plasma membrane

Depositor

Takeharu Nagai

Article

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern

Tags

HIS and Lyn

Expression

Mammalian

Mutation

S257G in Rluc8

Available Since

April 15, 2014

Availability

Academic Institutions and Nonprofits only
pKPY197

Plasmid

#62599

Purpose

Encodes Thr412Gly-CePheRS Alpha Subunit

Depositor

David Tirrell

Article

Yuet et al Proc Natl Acad Sci U S A. 2015 Feb 17. p

Insert

Thr412Gly-CePheRS Alpha Subunit

Tags

HIS

Expression

Worm

Mutation

Thr412Gly

Available Since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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pUCBB-ntH6-eGFP

Plasmid

#32557

Depositor

Claudia Schmidt-dannert

Article

Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

Insert

eGFP (eGFP )

Use

Synthetic Biology

Tags

6 His tag with Thrombin site

Expression

Bacterial

Promoter

Consitutive lac

Available Since

Oct. 18, 2011

Availability

Academic Institutions and Nonprofits only

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K401-iLid

Plasmid

#122484

Purpose

Component of optically-induced motor dimerization system

Depositor

Matt Thomson

Article

Ross et al Nature. 2019 Aug;572(7768):224-229. doi:

Insert

Kinesin 1-401 iLid His tag

Tags

His6 and iLid

Expression

Bacterial

Mutation

Kinesin amino acids 1-401

Promoter

T7

Available Since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 TFAM

Plasmid

#67064

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

TFAM (TFAM Human)

Use

Cell-free expression

Tags

EGFP and His

Mutation

S115C

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
NpT7-5-ERK1

Plasmid

#39229

Depositor

Melanie Cobb

Article

Robbins et al J Biol Chem. 1993 Mar 5;268(7):5097-106.

Insert

ERK1 (MAPK3 Human)

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

July 15, 2013

Availability

Academic Institutions and Nonprofits only
pQTEV-GYG

Plasmid

#31324

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

glycogenin (GYG1 Human)

Tags

His and TEV

Expression

Bacterial

Available Since

Nov. 20, 2012

Availability

Academic Institutions and Nonprofits only

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HM3C NaVAe1_p pET24b

Plasmid

#127854

Purpose

Bacterial expression vector for NaVAe1 bacterial sodium channel residues 143-288. It expresses HIStag-MBP-3C-channel protein.

Depositor

Dan Minor

Article

Arrigoni et al Cell. 2016 Feb 25;164(5):922-36. doi: 10

Insert

NaVAe1p

Tags

3C Cleavage site, His, and MBP

Expression

Bacterial

Available Since

May 15, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX-EGFAB(H306Y)

Plasmid

#25771

Depositor

Johann Deisenhofer

Article

McNutt et al J Biol Chem. 2009 Apr 17. 284(16):10561-

Insert

LDL receptor EGFAB (LDLR Human)

Tags

GST

Expression

Bacterial

Mutation

His 306 to Tyr

Available Since

Dec. 3, 2010

Availability

Academic Institutions and Nonprofits only
PM18033-PLS-KP1_rpoA-U

Plasmid

#190975

Purpose

Test the U-to-C editing activity of KP1 on AtrpoA editing site, in human cells

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP1

Tags

His

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 ATF1

Plasmid

#67065

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

ATF1 (ATF1 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pFR-mTq2-antiFRET-P2A-jRCaMP1b

Plasmid

#191468

Purpose

Compare the intensity of the genetically encoded calcium sensor (GECI) jRCaMP1b to mTurquoise2 fluorescent protein

Depositor

Dorus Gadella

Article

van der Linden et al eLife 14:RP105086; doi: https://doi.org/

Insert

jRCaMP1b

Tags

His-mTurquoise2-antiFRETlinker-P2A

Expression

Bacterial and Mammalian

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a_Pro-IL-36α

Plasmid

#214343

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL36A (IL36A Human)

Tags

His-TEV and Myc

Expression

Bacterial

Promoter

T7

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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SRS-MTBD(22/19), AB(monomer), wt

Plasmid

#22380

Depositor

Ron Vale

Article

Gibbons et al J Biol Chem. 2005 Jun 24. 280(25):23960-

Insert

Seryl tRNA synthetase (SRS) – mouse cytoplasmic dynein microtubule binding domain (MTBD) fusion protein

Tags

His and myc

Expression

Bacterial

Available Since

Nov. 24, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pET16b-Trx C35S

Plasmid

#36947

Depositor

Anna Blom

Article

King et al J Immunol. 2012 Apr 15;188(8):4103-12. E

Insert

Thioredoxin-1 C35S mutation (TXN Human)

Tags

His tag

Expression

Bacterial

Mutation

cys 35 to serine

Promoter

T7

Available Since

May 20, 2013

Availability

Academic Institutions and Nonprofits only
pEW4: pET-32a Dot1L(2-386)

Plasmid

#124099

Purpose

Dot1L(2-386) expression plasmid

Depositor

Cynthia Wolberger

Article

Worden et al Cell. 2019 Feb 8. pii: S0092-8674(19)301

Insert

Dot1L(2-386) (DOT1L Human)

Tags

Trx-His-TEV

Expression

Bacterial

Promoter

T7

Available Since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-PLS-KP6_rpoA-C

Plasmid

#190966

Purpose

Test the C-to-U editing activity of KP6 on AtrpoA editing site, in E. coli

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP6

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAD/CMV/V5-DEST-Rac1-2G

Plasmid

#66112

Purpose

Second generation Rac1 FRET biosensor for adenovirus production

Depositor

Olivier Pertz

Article

Fritz et al Dev Cell. 2015 Sep 29. pii: S1534-5807(1

Insert

Second generation Rac1 FRET biosensor

Use

Adenoviral

Tags

His-Myc

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
human b1 ecto-pIRES

Plasmid

#113387

Purpose

Express human beta 1 ectodomain with secretion peptide, purification tags, and C-terminal clasp

Depositor

Timothy Springer

Article

Li et al EMBO J. 2017 Mar 1;36(5):629-645. doi: 1

Insert

human beta 1 ectodomain

Tags

His tag

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 31, 2019

Availability

Academic Institutions and Nonprofits only

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pET22b-T5-FB-E25TAG-F6C

Plasmid

#176546

Purpose

Expresses IgG affinity protein FB(F6C) with a TAG stop codon at E25 position

Depositor

Han Xiao

Article

Yu et al Bioconjug Chem. 2018 Nov 21;29(11):3522-

Insert

IgG affinity protein

Tags

His tag

Expression

Bacterial

Mutation

change phenylalanine 6 to cysteine; change glutam…

Promoter

T5

Available Since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pQTEV-PRKRA

Plasmid

#31571

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

protein kinase, interferon-inducible double stranded RNA dependent activator (PRKRA Human)

Tags

His and TEV

Expression

Bacterial

Available Since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-Hook3 aa 1-160-GCN4

Plasmid

#74608

Purpose

Expresses artificially dimerized Hook domain of Hook3 in bacteria for purification

Depositor

Ron Vale

Article

Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

Insert

human Hook3 amino acids 1-160 (HOOK3 Human)

Tags

6x His, GCN4, Strep II, and superfolder GFP

Expression

Bacterial

Available Since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
spa-GCaMP6s

Plasmid

#67556

Purpose

photoactivatable fluorescent calcium reporter

Depositor

Ehud Isacoff

Article

Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

Insert

spa-GCaMP6s

Tags

HIS-tag

Expression

Mammalian

Mutation

K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…

Available Since

July 28, 2015

Availability

Academic Institutions and Nonprofits only
pET42b-A3A-PBE-ΔUGI

Plasmid

#119771

Purpose

To express the recombinant APOBEC3A in vitro

Depositor

Caixia Gao

Article

Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426

Insert

APOBEC3A-nCas9-NLS

Tags

6*HIS and NLS

Expression

Bacterial

Mutation

nCas9 has a mutation D10A in wild type SpCas9

Promoter

T7 promoter

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFPm-DAD M1041A

Plasmid

#25411

Depositor

Arthur Alberts

Article

Alberts et al J Biol Chem. 2001 Jan 26. 276(4):2824-30

Insert

Diap3 (Diaph3 Mouse)

Tags

EGFP, His, and Myc

Expression

Mammalian

Mutation

Alanine substitution at critical residue in DAD t…

Available Since

Oct. 6, 2014

Availability

Academic Institutions and Nonprofits only
pET28a-TmCA-P(11)4

Plasmid

#185783

Purpose

Encodes thermostable carbonic anhydrase enzyme linked to P(11)4 self-assembling peptide via a GS-linker sequence

Depositor

Lizhong He

Article

Shanbhag et al Adv. Funct. Mater. 2022, 32, 2108039

Insert

TmCA-P(11)4

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
PM18033-PLS-KP6_rpoA-U

Plasmid

#190987

Purpose

Test the U-to-C editing activity of KP6 on AtrpoA editing site, in human cells

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP6

Tags

His

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pEBG-TFII-I-delta N90

Plasmid

#22165

Depositor

Ananda Roy

Article

Sacristan et al J Biol Chem. 2004 Feb 20. 279(8):7147-58

Insert

TFII-I (GTF2I Human)

Tags

GST and His

Expression

Mammalian

Mutation

The first 90 aa of TFII-I are deleted

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only
mRFP-dmBACCS2

Plasmid

#67630

Purpose

Drosophila expression of mRFP-dmBACCS2, a Drosophila melanogaster blue light-activated Ca2+ channel switch

Depositor

Takao Nakata

Article

Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

Insert

mRFP-dmBACCS2

Use

Drosophila expression

Promoter

Drosophila actin gene 5C gene promoter

Available Since

Aug. 21, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pET14b_tev_PSGU

Plasmid

#59738

Purpose

Expresses a.a. 417-887 of human ZO-1

Depositor

Alan Fanning

Article

Nomme et al J Biol Chem. 2011 Dec 16;286(50):43352-6

Insert

zonula occludens-1 (TJP1 Human)

Tags

HIS tag and thrombin clvg

Expression

Bacterial

Promoter

T7

Available Since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only
pCellFree_G03 GATA1

Plasmid

#67068

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

GATA1 (GATA1 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pET-GST-TNRC6A-(935-984)

Plasmid

#42050

Depositor

Kumiko Ui-Tei

Article

Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

Insert

TNRC6A (TNRC6A Human)

Tags

GST and His

Expression

Bacterial

Mutation

contain aa935-984

Available Since

March 6, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-sCS4-2T

Plasmid

#28250

Depositor

Yawen Bai

Article

Zhou et al Nature. 2011 Apr 14. 472(7342):234-7.

Insert

single chain Cse4-Scm3-H4 with 2 Thrombin site

Tags

His

Expression

Bacterial

Mutation

A thrombin cutting site between Cse4 and Scm3. An…

Available Since

July 12, 2011

Availability

Academic Institutions and Nonprofits only
pET28a Gx-NL3

Plasmid

#169108

Purpose

Expresses protein G fused to 3 copies of NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.

Depositor

Maarten Merkx

Article

Wouters et al Bioconjug Chem. 2020 Mar 18;31(3):656-66

Insert

protein G - NanoLuc - NanoLuc - NanoLuc

Tags

his-tag and strep-tag

Expression

Bacterial

Mutation

Ala 24 stop

Promoter

T7

Available Since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDUAL_2_T5

Plasmid

#155175

Purpose

see pDUAL_T5: exchanged origin of replication (pBR322) and introduced bom site

Depositor

Bernd Nidetzky

Article

Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:

Inserts

Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
Cellobiose phosphorylase from Cellulomonas uda

Use

Synthetic Biology

Tags

His Tag and Strep - Tag II

Expression

Bacterial

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 23,191 results for: his