We narrowed to 7,526 results for: Ski;

1. pNAS1b split mCherry NEDD4 WW2 pos ctrl

   Plasmid

   #112029

   Purpose

   Mode #2 phosphosite positive control (split mCherry, using NEDD4 WW2 binding domain paired with proline-rich positive control peptide)

   Depositor

   Jesse Rinehart

   Article

   Barber et al Nat Biotechnol. 2018 Aug;36(7):638-644.

   Insert

   NEDD4 WW2 domain fused to C-terminal split mCherry, proline-rich pos ctrl interacting peptide fused to N-terminal split mCherry
   

   Tags

   Split mCherry

   Expression

   Bacterial

   Available Since

   Aug. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEX Abl-SH2 RK

   Plasmid

   #46403

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   Abl RK (Abl1 Mouse)
   

   Tags

   GST and Thrombin site

   Expression

   Bacterial

   Mutation

   contains amino acids 139-239

   Promoter

   Tac

   Available Since

   Aug. 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEX Jak1-SH2

   Plasmid

   #46447

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   Jak1 (JAK1 Human)
   

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 430-552

   Promoter

   Tac

   Available Since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEX PLCg2(NC)-SH2

   Plasmid

   #46472

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   PLCg2(NC) (PLCG2 Human)
   

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 524-749

   Promoter

   Tac

   Available Since

   Aug. 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPN186

   Plasmid

   #91573

   Purpose

   Express sgRNA targeting human AKT3

   Depositor

   Lindy Barrett

   Article

   Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

   Insert

   AKT3 (AKT3 Human)
   

   Use

   CRISPR

   Promoter

   U6

   Available Since

   Oct. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPHR-EYFP-hPMLIII

   Plasmid

   #103825

   Purpose

   Soluble BLInCR effector that is recruited to 'localizer' sites upon blue light illumination

   Depositor

   Karsten Rippe

   Article

   Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

   Insert

   PHR-EYFP-hPMLIII (CRY2, PML Mustard Weed, Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEX Grb10-SH2

   Plasmid

   #46437

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   Grb10 (GRB10 Human)
   

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 435-548

   Promoter

   Tac

   Available Since

   July 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPN008

   Plasmid

   #91576

   Purpose

   Express sgRNA targeting human BCL11A

   Depositor

   Lindy Barrett

   Article

   Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

   Insert

   BCL11A (BCL11A Human)
   

   Use

   CRISPR

   Promoter

   U6

   Available Since

   Oct. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pWzl_neo_DEST_flag_SOX2

   Plasmid

   #45309

   Depositor

   William Hahn

   Article

   Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

   Insert

   MCOPS3 (SOX2 Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   May 22, 2013

   Availability

   Academic Institutions and Nonprofits only

10. pPN187

    Plasmid

    #91574

    Purpose

    Express sgRNA targeting human AKT3

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

    Insert

    AKT3 (AKT3 Human)
    

    Use

    CRISPR

    Promoter

    U6

    Available Since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCIBN-TetR-tagRFP-T

    Plasmid

    #103809

    Purpose

    'Localizer' construct that marks tetO arrays and is targeted by a PHR-tagged effector upon illumination with blue light; can be visualized without triggering PHR recruitment

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    CIBN-TetR-tagRFP-T (CIB1, tetR Mustard Weed, E. coli)
    

    Expression

    Mammalian

    Mutation

    TetR: A4G (M2V)

    Promoter

    CMV

    Available Since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. mCherry-FKBP-TPD54(L53P,L67P)

    Plasmid

    #172458

    Purpose

    Expression of Tumor Protein D52 like 2 (TPD54/TPD52L2) with N-terminal mCherry-FKBP tag L53P,L67P mutant

    Depositor

    Stephen Royle

    Article

    Larocque et al J Cell Biol. 2021 Oct 4;220(10). pii: 21

    Insert

    TPD54 (TPD52L2 Human)
    

    Tags

    mCherry-FKBP

    Expression

    Mammalian

    Mutation

    changed Leucines 53 and 67 to proline (breaks coi…

    Promoter

    CMV

    Available Since

    Aug. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEX Nsp2-SH2

    Plasmid

    #46460

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Nsp2 (BCAR3 Human)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 144-259

    Promoter

    Tac

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEX6P1-HA-MEK1 C121S

    Plasmid

    #53164

    Purpose

    Expresses GST-HA tagged human MEK1 C121S from bacterial expression vector

    Depositor

    Christopher Counter

    Article

    Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

    Insert

    MEK1 (MAP2K1 Human)
    

    Tags

    GST and HA

    Expression

    Bacterial

    Mutation

    C121S

    Available Since

    June 9, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pPN094

    Plasmid

    #91621

    Purpose

    Express sgRNA targeting human GRIN2A

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

    Insert

    GRIN2A (GRIN2A Human)
    

    Use

    CRISPR

    Promoter

    U6

    Available Since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSUPER-retro-puro-tetO-Mek1-shRNA

    Plasmid

    #53179

    Purpose

    Expresses shRNA against mouse Mek1 from puromycin resistance retroviral vector

    Depositor

    Christopher Counter

    Article

    Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

    Insert

    MEK1 (Map2k1 Mouse)
    

    Use

    RNAi and Retroviral

    Expression

    Mammalian

    Available Since

    June 19, 2014

    Availability

    Academic Institutions and Nonprofits only

17. AIDx-ID-1058

    Plasmid

    #135347

    Purpose

    Expression of intradomain insertion of truncated human cytosine deaminase, hAIDx, at position 1058 of nSpyCas9 with uracil DNA glycosylase inhibitor (UGI) at the C terminus

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    nSpyCas9-hAIDx-ID-1058-UGI
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLJM1 Flag GFP 4E-BP1 Omp25

    Plasmid

    #112760

    Purpose

    express GFP-4EBP1-Omp25

    Depositor

    Roberto Zoncu

    Article

    Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

    Insert

    Flag-GFP-4EBP1-Omp25 (EIF4EBP1 Human)
    

    Use

    Lentiviral

    Tags

    Flag eGFP and Omp25

    Available Since

    Oct. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-ABEMAX-NLS-P2A_BLAST

    Plasmid

    #135355

    Purpose

    Expression of ABEMax (wildtype Tada monomer coupled with evolved Tada monomer) with dual C-terminal NLS in a single operon encoding P2A blasticidine resistance

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    ABEMax-2XNLS
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGEX chimerin2-SH2

    Plasmid

    #46416

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    chimerin2 (CHN2 Human)
    

    Tags

    AviTag, GST, and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 49-155

    Promoter

    Tac

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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