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Showing: 751 - 780 of 1463 results

  3. pDP1.2

    Plasmid
    #171092
    Purpose
    Expresses cell cycle-dependent OsTIR1 fused with mEmerald and Geminin (1-110). AKA ROLECCS G2(allows integration into AAVS1 safe-harbor)
    Depositor
    Dario Palmieri
    Article
    Capece et al Cell Death Differ. 2023 Aug 3. doi: 10.1
    Insert
    AAVS1-CMV-OsTIR1-mEmerald-Geminin-Puro-AAVS1
    Use
    Synthetic Biology
    Tags
    Emerald, Geminin
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    171092-plasmid-map-sequence-id-339101

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  5. pLentiDP1.3

    Plasmid
    #171096
    Purpose
    lentiviral construct (3rd generation) for the expression of cell cycle-dependent OsTIR1 fused with mEmerald and Cdt1 (AKA ROLECCS G1) and miniAID-fused mCherry2
    Depositor
    Dario Palmieri
    Article
    Capece et al Cell Death Differ. 2023 Aug 3. doi: 10.1
    Insert
    OsTIR1-mEmerald-Cdt1-P2A-miniAID-mCherry2
    Use
    Lentiviral and Synthetic Biology
    Tags
    Emerald, cdt1, miniAID, mCherry2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    171096-plasmid-map-sequence-id-339099

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  6. pLentiDP1.2

    Plasmid
    #171095
    Purpose
    lentiviral construct (3rd generation) for the expression of cell cycle-dependent OsTIR1 fused with mEmerald and Geminin (AKA ROLECCS G2) and miniAID-fused mCherry2
    Depositor
    Dario Palmieri
    Article
    Capece et al Cell Death Differ. 2023 Aug 3. doi: 10.1
    Insert
    OsTIR1-mEmerald-Geminin-P2A-miniAID-mCherry2
    Use
    Lentiviral and Synthetic Biology
    Tags
    Emerald, Geminin, miniAID, mCherry2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    171095-plasmid-map-sequence-id-339475

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  7. LV-miR-142

    Plasmid
    #117056
    Purpose
    Overexpression of miR-142-3p using miR-451 backbone to enable DICER-independent expression along with orange fluorescent protein (OFP) to track miRNA expression
    Depositor
    Michele De Palma
    Article
    Baer et al Nat Cell Biol. 2016 Jul;18(7):790-802. d
    Insert
    miR-142-3p - OFP (Mir142 Mouse)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    SFFV promoter
    Available since
    Oct. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    117056-plasmid-map-sequence-id-223999

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  12. pb-NES-ZapCV5 (cpV143)

    Plasmid
    #112061
    Purpose
    Genetically-encoded Cyan-Yellow Zinc FRET sensor. Localizes to the cytosol. Low affinity to free zinc (Kd ~ 300nM, n = 0.55) due to mutation of the zinc binding Cys residues to His.
    Depositor
    Amy Palmer
    Article
    Fiedler et al Anal Chem. 2017 Jan 3;89(1):711-719. doi
    Insert
    NES-ZapCV5
    Use
    Tags
    Nuclear Export Signal (NES) derived from HIV-1
    Expression
    Mammalian
    Mutation
    ECFP gene is truncated 36 bp at 3' end. Venu…
    Promoter
    CBA
    Available since
    July 9, 2018
    Availability
    Academic Institutions and Nonprofits only

  13. pcDNA3.1(+)-NES-ZapCV2 (cpV143)

    Plasmid
    #112060
    Purpose
    Genetically-encoded Cyan-Yellow Zinc FRET sensor. Localizes to the cytosol. Useful for detecting free zinc ion levels in the cytosol (in vitro Kd ~2.3 nM, n = 0.53)
    Depositor
    Amy Palmer
    Article
    Fiedler et al Anal Chem. 2017 Jan 3;89(1):711-719. doi
    Insert
    NES-ZapCV2
    Use
    Tags
    Nuclear Export Signal (NES) derived from HIV-1
    Expression
    Mammalian
    Mutation
    ECFP gene is truncated 36 bp at 3' end. Venu…
    Promoter
    CMV
    Available since
    July 25, 2018
    Availability
    Academic Institutions and Nonprofits only
    112060-plasmid-map-sequence-id-216934

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  15. PB-NES-ZapCV2

    Plasmid
    #222950
    Purpose
    Genetically-encoded Cytosolic Cyan-Yellow Zinc FRET sensor. Useful for detecting free zinc ion levels in the cytosol (in vitro Kd ~2.3 nM, n = 0.53)
    Depositor
    Amy Palmer
    Article
    Fiedler et al Anal Chem. 2017 Jan 3;89(1):711-719. doi
    Insert
    NES-ZapCV2 (NES Synthetic)
    Use
    Tags
    ECFP (Enhanced Cyan Fluorescent Protein), Nuclear…
    Expression
    Mammalian
    Mutation
    ECFP gene is truncated 36 bp at 3' end. Venu…
    Promoter
    Available since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    222950-plasmid-map-sequence-id-450583

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  16. MPP7

    Plasmid
    #28149
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    membrane protein, palmitoylated 7 (MPP7 Human)
    Use
    Tags
    His tag with thrombin cleavage
    Expression
    Bacterial
    Mutation
    PDZ Domain (residues 135-225)
    Promoter
    Available since
    Feb. 28, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 751 - 780 of 1463 results