We narrowed to 17,788 results for: Por

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pCMV-deltaR8.2(A14C,E45C)

Plasmid

#79047

Purpose

mammalian expression, lentiviral packaging

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-deltaR8.2(A14C,E45C,A92E)

Plasmid

#79048

Purpose

mammalian expression, lentiviral packaging

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2017

Availability

Academic Institutions and Nonprofits only

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pAG25-L-IFP-F[1] (clon 3)

Plasmid

#52899

Purpose

Plasmid template for PCR of cassettes for HR, used to introduce IFP F[1] fragments 3’ to the ORF of the genes to study. Plasmid confers ampicillin resistance to DH5α.

Depositor

Stephen Michnick

Article

Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

Insert

L-IFP-F[1]

Use

Vector for homologous recombination

Promoter

TEF promoter

Available Since

June 12, 2014

Availability

Academic Institutions and Nonprofits only
pAG32-L-IFP-F[2] (clon 3)

Plasmid

#52900

Purpose

Plasmid template for PCR of cassettes for HR, used to introduce IFP F[2] fragments 3’ to the ORF of the genes to study. Plasmid confers ampicillin resistance to DH5α.

Depositor

Stephen Michnick

Article

Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

Insert

L-IFP-F[2]

Use

Vector for homologous recombination

Promoter

TEF promoter

Available Since

June 5, 2014

Availability

Academic Institutions and Nonprofits only
pCL1316

Plasmid

#217958

Purpose

Expression of human H4 tagged on its C-terminus with eGFP. Expression in mammalian cells driven by the CMV promoter.

Depositor

Andrew Bowman

Article

Pardal et al Elife. 2022 Sep 6;11:e81755. doi: 10.755

Insert

Histone H4 (H4C1 Human)

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

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pLpC-L-IFP-F[1] (mammalian)

Plasmid

#52901

Purpose

Binary retroviral pl. (puromycin). MCS:(GGGGS)X 2 + IFP-F[1] (ClaI/EcoRI). Insert can be subcloned between the MCS. Plasmid confers ampicillin resistance to DH5α.

Depositor

Stephen Michnick

Article

Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

Insert

L-IFP-F[1]

Use

Retroviral

Expression

Mammalian

Promoter

Unknown

Available Since

June 5, 2014

Availability

Academic Institutions and Nonprofits only
pLpC-L-IFP-F[2] (mammalian)

Plasmid

#52902

Purpose

Binary retroviral pl. (puromycin). MCS:(GGGGS)X 2 + IFP-F[2] (ClaI/EcoRI). Insert can be subcloned between the MCS. Plasmid confers ampicillin resistance to DH5α.

Depositor

Stephen Michnick

Article

Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

Insert

L-IFP-F[2]

Use

Retroviral

Expression

Mammalian

Promoter

Unknown

Available Since

June 5, 2014

Availability

Academic Institutions and Nonprofits only
pVHed02

Plasmid

#82352

Purpose

Bxb1/TP901 temporal logic gate with mKate2 and sfGFP on low-copy plasmid

Depositor

Richard Murray

Article

Hsiao et al Mol Syst Biol. 2016 May 17;12(5):869. do

Inserts

mKate2 RFP
Superfolder GFP
Temporal logic gate with TP901 and Bxb1 attachment sites

Use

Synthetic Biology

Expression

Bacterial

Available Since

Dec. 19, 2016

Availability

Academic Institutions and Nonprofits only

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KBB285

Plasmid

#185076

Purpose

RFA3-GFP fusion

Depositor

Ken Belanger

Article

Belanger et al J Biol Chem. 2004 Oct 15;279(42):43530-9

Insert

RFA3

Tags

GFP

Expression

Yeast

Available Since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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KBB284

Plasmid

#185075

Purpose

RFA2-GFP fusion

Depositor

Ken Belanger

Article

Belanger et al J Biol Chem. 2004 Oct 15;279(42):43530-9

Insert

RFA2

Tags

GFP

Expression

Yeast

Available Since

July 21, 2022

Availability

Academic Institutions and Nonprofits only

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pDHt-KS

Plasmid

#63616

Purpose

The multiple cloning site of pGreenII0000 cloned into binary vector pDHt

Depositor

Seogchan Kang

Article

Khang et al Fungal Genet Biol. 2005 Jun;42(6):483-92

Type

Empty backbone

Use

Fungal expression

Available Since

July 27, 2015

Availability

Academic Institutions and Nonprofits only

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pdelta105-ABCb10-GFP

Plasmid

#19034

Depositor

Orian Shirihai

Article

Graf et al J Biol Chem. 2004 Oct 8. 279(41):42954-6

Insert

ABCb10 lacking the mitochondrial targetting sequence

Tags

GFP

Expression

Mammalian

Available Since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only
insulator_seq_UENU_donor

Plasmid

#221459

Purpose

Vector from which reporter gene 3'UTRs, an enhancer, and a neutral DNA spacer are cloned for assembling an insulator-seq reporter library.

Depositor

Maria Cristina Gambetta

Article

Tonelli et al STAR Protoc. 2024 Oct 24;5(4):103391. do

Insert

3'UTR-OpIE2 enhancer-neutral DNA spacer-3'UTR

Use

Synthetic Biology

Available Since

Nov. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pro::EX1-2:GFP

Plasmid

#218565

Purpose

Transcriptional reporter for ARF7 promoter

Depositor

Anthony Bishopp

Insert

AT5G20730 (NPH4 Mustard Weed)

Expression

Plant

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pro::EX1:GFP

Plasmid

#218564

Purpose

Transcriptional reporter for ARF7 promoter

Depositor

Anthony Bishopp

Insert

AT5G20730 (NPH4 Mustard Weed)

Expression

Plant

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pro::GFP

Plasmid

#218563

Purpose

Transcriptional reporter for ARF7 promoter

Depositor

Anthony Bishopp

Insert

AT5G20730 (NPH4 Mustard Weed)

Expression

Plant

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV128-Rluc-CTE-GAG_O

Plasmid

#147108

Purpose

Mammalian Expression of Rluc-CTE-GAG

Depositor

Elisa Izaurralde

Article

Teplova et al Nat Struct Mol Biol. 2011 Aug 7;18(9):99

Insert

Rluc-CTE-GAG

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pLNHA-C1-HIV-Rev-M10_O

Plasmid

#147112

Purpose

Mammalian Expression of HIV-Rev-M10

Depositor

Elisa Izaurralde

Article

Teplova et al Nat Struct Mol Biol. 2011 Aug 7;18(9):99

Insert

HIV-Rev-M10

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HIV-Rev-M10_O

Plasmid

#147114

Purpose

Mammalian Expression of HIV-Rev-M10

Depositor

Elisa Izaurralde

Article

Teplova et al Nat Struct Mol Biol. 2011 Aug 7;18(9):99

Insert

HIV-Rev-M10

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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KBB329

Plasmid

#185077

Purpose

NUP82 expression in yeast

Depositor

Ken Belanger

Article

Belanger et al J Biol Chem. 2004 Oct 15;279(42):43530-9

Insert

NUP82

Expression

Yeast

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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KBB382

Plasmid

#185085

Purpose

HA-tagged POM152 with glycosylation sites mutated

Depositor

Ken Belanger

Article

Belanger et al Genetics. 2005 Nov;171(3):935-47. doi: 1

Insert

POM152

Tags

HA

Expression

Yeast

Mutation

Pom152 N280A, N398A, N???A, N???A = Pom152Dglyc

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only
pVHed07

Plasmid

#82350

Purpose

Bxb1/TP901 temporal logic gate with mKate2 and sfGFP in Phi80 chromosomal integration plasmid

Depositor

Richard Murray

Article

Hsiao et al Mol Syst Biol. 2016 May 17;12(5):869. do

Inserts

mKate2 RFP
Superfolder GFP
Temporal logic gate with TP901 and Bxb1 attachment sites

Use

Synthetic Biology

Expression

Bacterial

Available Since

Jan. 20, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE-mt-mKeima

Plasmid

#131626

Purpose

lentiviral expression of mitochondrial mKeima for generation of stable cell lines

Depositor

Richard Youle

Article

Vargas et al Mol Cell. 2019 Apr 18;74(2):347-362.e6.

Insert

mt-mKeima

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-HIFR

Plasmid

#192946

Purpose

Lentiviral production of HIF-1alpha reporter

Depositor

Markus Covert

Article

Jeknic et al J Biol Chem. 2023 Mar 10:104599. doi: 10

Insert

HIF-1alpha reporter

Use

Lentiviral

Promoter

mCMV

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-ADP-MCS-FLAG

Plasmid

#192360

Purpose

Adipocyte-specific overexpression of genes

Depositor

Yifu Qiu

Article

Xue et al Cell Metab. 2022 Sep 6;34(9):1325-1341.e

Type

Empty backbone

Use

AAV

Tags

3xflag

Expression

Mammalian

Promoter

adiponectin promoter

Available Since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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NM9-SpCas9-NLS3

Plasmid

#128177

Purpose

Expresses R. toruloides codon-optimized SpCas9 under PGK1 promoter

Depositor

Huimin Zhao

Article

Schultz et al Biotechnol Bioeng. 2019 Apr 30. doi: 10.

Insert

SpCas9-NLS3

Use

CRISPR

Expression

Yeast

Promoter

pPGK1

Available Since

April 1, 2020

Availability

Academic Institutions and Nonprofits only

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NM1-5S-tRNA-SgH

Plasmid

#128178

Purpose

Expresses gRNA using fusion of R. toruloides 5S rRNA and tRNA-Arg as promoter

Depositor

Huimin Zhao

Article

Schultz et al Biotechnol Bioeng. 2019 Apr 30. doi: 10.

Insert

gRNA cloning cassette

Use

CRISPR

Expression

Yeast

Promoter

5S rRNA-tRNA(Arg)

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a_T7-ARG2

Plasmid

#106474

Purpose

Expresses acoustic reporter genes or gas vesicles (ARG2) in Escherichia coli

Depositor

Mikhail Shapiro

Article

Bourdeau et al Nature. 2018 Jan 3;553(7686):86-90. doi:

Insert

ARG2

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7

Available Since

March 23, 2018

Availability

Academic Institutions and Nonprofits only

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pAcBac1-3nitroY-A7-RS

Plasmid

#141173

Purpose

Expression of orthogonal tRNA and tRNA synthetase for incorporation of unnatural amino acids

Depositor

Ryan Mehl

Article

Porter et al ACS Chem Biol. 2019 Jun 21;14(6):1328-13

Insert

A7 3nY tRNA synthetase

Use

Lentiviral

Expression

Mammalian

Mutation

P186T L274R N311G C313N

Promoter

CMV

Available Since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a_T5-ARG1

Plasmid

#106476

Purpose

Expresses acoustic reporter genes or gas vesicles (ARG1) in Escherichia coli Nissle 1917

Depositor

Mikhail Shapiro

Article

Bourdeau et al Nature. 2018 Jan 3;553(7686):86-90. doi:

Insert

ARG1

Use

Synthetic Biology

Expression

Bacterial

Promoter

T5

Available Since

March 23, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 iTEV

Plasmid

#64277

Purpose

Expresses infrared fluorescent tobacco etch virus protease sensor (iTEV)

Depositor

Xiaokun Shu

Article

To et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

Insert

Infrared fluorescent tobacco etch virus protease sensor (iTEV)

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pTD103-ARG1

Plasmid

#106475

Purpose

Expresses acoustic reporter genes or gas vesicles (ARG1) in Salmonella typhimurium

Depositor

Mikhail Shapiro

Article

Bourdeau et al Nature. 2018 Jan 3;553(7686):86-90. doi:

Insert

ARG1

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLux

Available Since

Aug. 27, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
TPC1-G-GECO1.2

Plasmid

#207143

Purpose

Fusion of Ca2+ reporter and endosomal Ca2+-permeable channel

Depositor

Antony Galione

Article

Davis et al EMBO J. 2020 Jul 15;39(14):e104058. doi:

Insert

TPCN1 (TPCN1 Human)

Tags

G-GECO1.2

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pTK305

Plasmid

#66812

Purpose

IVT vector for Fluc mRNA

Depositor

Ron Weiss

Article

Andries et al J Control Release. 2015 Sep 2. pii: S016

Insert

Firefly luciferase

Use

Luciferase; Mrna ivt

Promoter

T7

Available Since

Sept. 8, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-TetO(3G)-WPRE

Plasmid

#186206

Purpose

tTA dependent gene expression vector

Depositor

Naoshige Uchida

Article

Amo et al Nat Neurosci. 2022 Aug;25(8):1082-1092.

Type

Empty backbone

Use

AAV

Promoter

TetO(3G)

Available Since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-C1-Nup35

Plasmid

#87342

Purpose

To express human Nup35 in mammalian cells

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

nucleoporin 35 (NUP35 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-NP-B/BRISBANE/60/2008

Plasmid

#187921

Purpose

Expresses nucleoprotein of Influenza B/Brisbane/60/2008 virus in mammalian cells

Depositor

Arindam Mondal

Article

Kedia et al Front Microbiol. 2022 May 25;13:868367.

Insert

Influenza B Nucleoprotein

Tags

UNTAGGED

Expression

Mammalian

Promoter

CMV Promoter

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
4xHRE_RE:RedF

Plasmid

#178314

Purpose

HRE RedFirefly luciferase reporter

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Methods Mol Biol. 2022;2524:409-432. doi

Insert

HRE RedFirefly reporter

Use

Luciferase and Synthetic Biology

Expression

Mammalian

Available Since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
3xDBE_RE:NLuc

Plasmid

#178318

Purpose

FOXO NLuc luciferase reporter

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Methods Mol Biol. 2022;2524:409-432. doi

Insert

FOXO NLuc Reporter

Use

Luciferase and Synthetic Biology

Expression

Mammalian

Available Since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
GAG-Pan

Plasmid

#99640

Purpose

EGFP-mCherry Met71GAG reporter for methionine misincorporation

Depositor

Tao Pan

Article

Gomes et al RNA. 2016 Mar;22(3):467-76. doi: 10.1261

Insert

mCherry

Tags

EGFP

Expression

Mammalian

Mutation

Met71 mutated to GAG (Glu)

Promoter

CMV

Available Since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 1001 - 1040 of 17788 results

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We narrowed to 17,788 results for: Por