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We narrowed to 1,807 results for: tet on vector

Showing: 1251 - 1300 of 1807 results

  5. pET His6 MBP TEV HDAC8_374

    Plasmid
    #122174
    Purpose
    Expresses a truncated construct of histone deacetylase 8 for crystallography bearing an N-terminal tobacco etch virus protease-cleavable hexahistidine-maltose binding protein tag
    Depositor
    David Christianson
    Article
    Porter et al ACS Chem Biol. 2017 Sep 15;12(9):2281-22
    Insert
    His6 MBP TEV HDAC8_374 (HDAC8 Human)
    Use
    Tags
    hexahistidine tag and tobacco etch virus protease…
    Expression
    Bacterial
    Mutation
    Residues Met1–Pro7 and H375-V377 have been delete…
    Promoter
    T7
    Available since
    March 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    122174-plasmid-map-sequence-id-237469

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  10. pCW-Cas9-Blast

    Plasmid
    #83481
    Purpose
    Lentiviral vector for mammalian expression of doxycycline-inducible Cas9 and constitutive expression of Blasticidin S deaminase -T2A -rtTA
    Depositor
    Mohan Babu
    Inserts
    SpCas9
    Blasticidin S deaminase -T2A - reverse tetracycline-controlled transactivator
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Replaced puromycin N-acetyltransferase on the ori…
    Promoter
    Tight Tre and human phosphoglycerate kinase 1 pro…
    Available since
    Nov. 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    83481-plasmid-map-sequence-id-335118

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  13. pX131

    Plasmid
    #167149
    Purpose
    MoClo-compatible Level 0-SP promoterless vector encoding Aspergillus nidulans 4'-phosphopantetheinyl transferase NpgA codon-optimised for expression in Nicotiana benthamiana and Pichia pastoris
    Depositor
    Karen Sarkisyan
    Article
    Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103
    Insert
    4'-phosphopantetheinyl transferase, npgA
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    167149-plasmid-map-sequence-id-335232

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  22. pAX2

    Plasmid
    #117398
    Purpose
    allelic exchange vector with GFP merodiploid tracker, temperature-sensitive origin of replication, and TetR-controlled kill switch
    Depositor
    Karen Guillemin
    Article
    Wiles et al MBio. 2018 Oct 9;9(5). pii: mBio.01877-1
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    117398-plasmid-map-sequence-id-230535

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  23. pFTK055

    Plasmid
    #171327
    Purpose
    Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.
    Depositor
    Arnold Driessen
    Article
    Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021
    Insert
    rTA2S-M2 (TetR-3xVP16) transcriptional activator
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    n/a
    Promoter
    Available since
    Jan. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    171327-plasmid-map-sequence-id-351262

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  26. pAH-CTX1-rha

    Plasmid
    #129390
    Purpose
    Derived from mini-CTX1. An integrase vector that can integrate the rhamnose-inducible promoter system into the chromosome of a host bacterium.
    Depositor
    Silvia Cardona
    Article
    Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021
    Insert
    Rhamnose-inducible promoter
    Use
    Tags
    Expression
    Bacterial
    Mutation
    N/A
    Promoter
    PrhaBAD
    Available since
    Dec. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    129390-plasmid-map-sequence-id-266815

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  33. pCVD518

    Plasmid
    #66158
    Purpose
    Six copies of the 415 bp PstI fragment of pCVD512, carrying the tdh1 gene probe from Vibrio parahaemolyticus WP1, for generation of probe
    Depositor
    James Kaper
    Article
    Nishibuchi et al Infect Immun. 1985 Sep;49(3):481-6.
    Insert
    tdh1
    Use
    Cloning vector
    Tags
    Expression
    Mutation
    Promoter
    Unknown
    Available since
    July 8, 2015
    Availability
    Academic Institutions and Nonprofits only

  36. pCW-ND-Cdt1ΔR198A/R210A-HA-Puro

    Plasmid
    #193769
    Purpose
    Doxycyline-inducible (TetOn) human Cdt1 (SV40 NLS , C-terminal HA ) w/ mutations stopping S phase degradation and in MCM binding (R198A/R210A), expressed from all-in-one pCW vector (TetOn + rtTA)
    Depositor
    Tobias Meyer
    Article
    Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do
    Insert
    ND-Cdt1ΔR198A/R210A (CDT1 Human)
    Use
    Lentiviral
    Tags
    HA
    Expression
    Mutation
    amino acids 1-19 deleted, ΔCy mutation (aa68-70 t…
    Promoter
    TRE
    Available since
    Jan. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    193769-plasmid-map-sequence-id-380211

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  37. pHOT-P2Y2R

    Plasmid
    #159108
    Purpose
    entiviral vector encoding P2Y purinoceptor 2 (P2Y2R), a high affinity G-protein coupled receptor that mobilizes Ca2+ from intracellular stores upon binding extracellular ATP.48,49 By using the Gibson
    Depositor
    Fabio Mammano
    Article
    Mazzarda et al Lab Chip. 2020 Jul 23. doi: 10.1039/d0lc
    Insert
    P2Y2R
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    UBC
    Available since
    Jan. 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    159108-plasmid-map-sequence-id-318664

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  38. pTU1-A-fbp_RiboJ_GFP+_MGApt_Bba_B0015

    Plasmid
    #107578
    Purpose
    B. megaterium DSM319 fbp promoter, GFP+ with malachite green mRNA aptamer for Bacillus cell-free transcription-translation. Bacillus shuttle vector backbone, colE1/AmpR (E. coli), RebB/TetA (Bacillus)
    Depositor
    Paul Freemont
    Article
    Moore et al Proc Natl Acad Sci U S A. 2018 Apr 17. p
    Insert
    GFP+
    Use
    Synthetic Biology
    Tags
    B. megaterium DSM319 xylA leader sequence (MTSSKI…
    Expression
    Bacterial
    Mutation
    F64L/ S65T/ Q80R/ F99S/ M153T/ V163A
    Promoter
    fbp promoter Bacillus megaterium DSM319
    Available since
    April 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    107578-plasmid-map-sequence-id-209151

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  39. pCW-ND-Cdt1Δ499-546-HA-Puro

    Plasmid
    #193768
    Purpose
    Doxycyline-inducible (TetOn) human Cdt1 (SDV40 NLS , C-terminal HA ) w/ mutations stopping S phase degradation and in MCM binding domain (Δ499-546), expressed from all-in-one pCW vector (TetOn + rtTA)
    Depositor
    Tobias Meyer
    Article
    Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do
    Insert
    ND-Cdt1Δ499-546 (CDT1 Human)
    Use
    Lentiviral
    Tags
    HA
    Expression
    Mutation
    amino acids 1-19 deleted, ΔCy mutation (aa68-70 t…
    Promoter
    TRE
    Available since
    Jan. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    193768-plasmid-map-sequence-id-380214

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  41. pJB158

    Plasmid
    #202601
    Purpose
    RP4-based mobilizable plasmid for the IPTG inducible expression of inserts that can be introduced using BsaI-based Golden Gate cloning. Vector has two origins of replication.
    Depositor
    Athanasios Typas
    Article
    Bobonis et al Nat Protoc. 2024 May 9. doi: 10.1038/s41
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    202601-plasmid-map-sequence-id-412308

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  42. pFP.R274

    Plasmid
    #138223
    Purpose
    Expresses the split mCherry reporter interrupted by the TvoVMA intein (in cis)
    Depositor
    Baojun Wang
    Article
    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:
    Inserts
    araC (araC )
    mCherryN(GSK).TvoVMA.(TVI)mCherryC.H6
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    P(araBAD) and iPC
    Available since
    May 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    138223-plasmid-map-sequence-id-296592

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  43. pFP.R275

    Plasmid
    #138224
    Purpose
    Expresses the split mCherry reporter interrupted by the PhoRadA intein (in cis)
    Depositor
    Baojun Wang
    Article
    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:
    Inserts
    araC (araC )
    mCherryN(SGK).PhoRadA.(TQL)mCherryC.H6
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    P(araBAD) and iPC
    Available since
    March 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    138224-plasmid-map-sequence-id-273527

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Showing: 1251 - 1300 of 1807 results