We narrowed to 13,477 results for: crispr cas9

1. pSpCas9n(BB)-2A-Puro (PX462)

   Plasmid

   #48141

   Purpose

   NOTE: A new version of this plasmid is now available. See Addgene plasmid 62987

   Depositor

   Feng Zhang

   Article

   Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

   Insert

   hSpCas9n-2A-Puro
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Mutation

   Cas9 D10A nickase mutant

   Promoter

   Cbh

   Available Since

   Oct. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

2. pDT-SuperFi-Cas9

   Plasmid

   #183630

   Purpose

   Expression and purification of N-terminal His-MBP tagged SuperFi Cas9 in E. coli

   Depositor

   David Taylor

   Article

   Bravo et al Nature. 2022 Mar 2. pii: 10.1038/s41586-

   Insert

   SuperFi-Cas9
   

   Use

   CRISPR

   Tags

   6xHis-MBP

   Expression

   Bacterial

   Mutation

   Y1010D, Y1013D, Y1016D, V1018D, R1019D, Q1027D, …

   Promoter

   T7

   Available Since

   May 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCW57-GFP-2A-Cas9

   Plasmid

   #170805

   Purpose

   All-in-one doxycycline inducible lentiviral vector for expression of Cas9 in combination with turbo GFP using the P2A self-cleaving peptide

   Depositor

   Morten Frodin

   Article

   Niu et al Nat Genet. 2022 Dec 5. doi: 10.1038/s415

   Insert

   Cas9
   

   Use

   CRISPR and Lentiviral

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. NLS-SpCas9mSA-His

   Plasmid

   #182951

   Purpose

   Express SpCas9mSA in bacteria

   Depositor

   Wen Xue

   Article

   Liang et al Nat Commun. 2022 Jan 21;13(1):437. doi:

   Insert

   NLS-SpCas9mSA-His
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX330a dCas9-LSD1

   Plasmid

   #92362

   Purpose

   Modified CAG promoter-containing vector for ubiquitous expression of catalytically inactive Cas9 fused to LSD1. For targeted enhancer demethylation in chicken embryos.

   Depositor

   Tatjana Sauka-Spengler

   Article

   Williams et al Development. 2018 Feb 23;145(4). pii: de

   Insert

   dCas9-LSD1
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Aug. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG Cas9-2A-Citrine

   Plasmid

   #92358

   Purpose

   CAG-driven ubiquitous expression of Cas9. Contains 2A-Citrine reporter. For CRISPR mediated gene knockouts in chicken embryos.

   Depositor

   Tatjana Sauka-Spengler

   Article

   Williams et al Development. 2018 Feb 23;145(4). pii: de

   Insert

   Cas9 2A Citrine
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-CASI-Cas9C

   Plasmid

   #80932

   Purpose

   Expresses Cas9C in mammalian cells; derived from pZac2.1 with CASI promoter.

   Depositor

   George Church

   Article

   Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

   Insert

   Cas9C
   

   Use

   AAV, CRISPR, and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. G10 Cas9 MDC123

   Plasmid

   #59187

   Purpose

   G10 promoting Glycing Max codon optimized Cas9 (N and C terminus NLS) with bar plant selection

   Depositor

   Robert Stupar

   Article

   Michno et al GM Crops Food. 2015;6(4):243-52. doi: 10

   Insert

   Glycine Max codon optimized Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   G10

   Available Since

   Dec. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. HiFi dCas9 VPR

   Plasmid

   #188510

   Purpose

   Expresses FLAG tagged HiFi dCas9 VPR

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A/R691A/H840A

   Promoter

   EF1a

   Available Since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMK-Cas9-gate

    Plasmid

    #113743

    Purpose

    Gateway destination vector encoding Maize ubiquitin promoter driving Cas9 and 35S promoter driving aminoglycoside phosphotransferase for G418 selection in plants.

    Depositor

    Magdalena Bezanilla

    Article

    Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

    Insert

    Cas9
    

    Use

    CRISPR; Gateway destination vector

    Tags

    Expression

    Mutation

    Promoter

    Z. Mays ubiquitin promoter, 35S promoter

    Available Since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. HiFi dCas9 KRAB

    Plasmid

    #188500

    Purpose

    Expresses FLAG tagged HiFi dCas9 KRAB

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A/R691A/H840A

    Promoter

    EF1a

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pYPQ-dpcoCas9-EV2.1

    Plasmid

    #158411

    Purpose

    CRISPR-dpcoCas9 fused with EDLL activator for transcriptional activation

    Depositor

    Yiping Qi

    Article

    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

    Insert

    dpcoCas9-EDLL-T2A-MS2-VPR(VP64-p65-Rta)-NOS-ter
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pVMG0173_Cq-Actin5c-Cas9

    Plasmid

    #169345

    Purpose

    Expression of Cas9 in Culex quinquefasciatus or other mosquitoes

    Depositor

    Valentino Gantz

    Article

    Feng et al Nat Commun. 2021 May 20;12(1):2960. doi:

    Insert

    pVMG0173_Cq-Actin5c-Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    CPIJ009808

    Available Since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pVMG0212_Cq-nanos-Cas9

    Plasmid

    #169348

    Purpose

    Expression of Cas9 in Culex quinquefasciatus or other mosquitoes

    Depositor

    Valentino Gantz

    Article

    Feng et al Nat Commun. 2021 May 20;12(1):2960. doi:

    Insert

    pVMG0212_Cq-nanos-Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    CPIJ011551

    Available Since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pVMG0213_Cq-vasa-Cas9

    Plasmid

    #169347

    Purpose

    Expression of Cas9 in Culex quinquefasciatus or other mosquitoes

    Depositor

    Valentino Gantz

    Article

    Feng et al Nat Commun. 2021 May 20;12(1):2960. doi:

    Insert

    pVMG0213_Cq-vasa-Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    CPIJ009286

    Available Since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCas9-EcRT-Z3-Kan

    Plasmid

    #232096

    Purpose

    Yeast CEN plasmid for estradiol-inducible expression of spCas9 and Ec86 reverse transcriptase.

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

    Insert

    Z3 promoter and Z3EV transcription factor
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Z3

    Available Since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pVMG0193_Cq-Rpl40-Cas9

    Plasmid

    #169346

    Purpose

    Expression of Cas9 in Culex quinquefasciatus or other mosquitoes

    Depositor

    Valentino Gantz

    Article

    Feng et al Nat Commun. 2021 May 20;12(1):2960. doi:

    Insert

    pVMG0193_Cq-Rpl40-Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    CPIJ002413

    Available Since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. PB-iCas9

    Plasmid

    #160048

    Purpose

    inducible expression of Cas9

    Depositor

    Xiaojun Lian

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available Since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKMW124_dCas9

    Plasmid

    #244821

    Purpose

    Mammalian expression of catalytically inactive Cas9 (dCas9)

    Depositor

    Jennifer Doudna

    Article

    Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

    Insert

    dCas9 (WT)
    

    Use

    CRISPR

    Tags

    3xFLAG-SV40 NLS and Nucleoplasmin NLS

    Expression

    Mammalian

    Mutation

    D10A/H840A

    Promoter

    CAG, U6

    Available Since

    Sept. 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHS9_10xHis_MBP_TEV_dCas9

    Plasmid

    #244831

    Purpose

    Bacterial expression of catalytically inactive Cas9 (dCas9) for protein purification

    Depositor

    Jennifer Doudna

    Article

    Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

    Insert

    dCas9 (WT)
    

    Use

    CRISPR

    Tags

    10xHis-MBP

    Expression

    Bacterial

    Mutation

    D10A/H840A

    Promoter

    T7

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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