We narrowed to 1,601 results for: tef

1. THI VP64 pGAP T3

   Plasmid

   #161490

   Purpose

   CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_THI11_T3_gRNA_MS2

   Depositor

   Brigitte Gasser, Diethard Mattanovich

   Article

   Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

   Inserts

   dCAS9
   MS2-VP64
   gRNA (Targeting T3 in the THI11 promoter)
   

   Expression

   Yeast

   Promoter

   pGAP, pPOR1, and pTEF2

   Available Since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. THI VP64 pGAP T4

   Plasmid

   #161491

   Purpose

   CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_THI11_T4_gRNA_MS2

   Depositor

   Brigitte Gasser, Diethard Mattanovich

   Article

   Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

   Inserts

   dCAS9
   MS2-VP64
   gRNA (Targeting T4 in the THI11 promoter)
   

   Expression

   Yeast

   Promoter

   pGAP, pPOR1, and pTEF2

   Available Since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. RIB VP64 R2

   Plasmid

   #161494

   Purpose

   CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_RIB1_R2_gRNA_MS2

   Depositor

   Brigitte Gasser, Diethard Mattanovich

   Article

   Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

   Inserts

   dCAS9
   MS2-VP64
   gRNA (Targeting R2 in the RIB1 promoter)
   

   Expression

   Yeast

   Promoter

   pGAP, pPOR1, and pTEF2

   Available Since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. RIB VP64 R3

   Plasmid

   #161495

   Purpose

   CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_RIB1_R3_gRNA_MS2

   Depositor

   Brigitte Gasser, Diethard Mattanovich

   Article

   Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

   Inserts

   dCAS9
   MS2-VP64
   gRNA (Targeting R3 in the RIB1 promoter)
   

   Expression

   Yeast

   Promoter

   pGAP, pPOR1, and pTEF2

   Available Since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. RIB VP64 R4

   Plasmid

   #161496

   Purpose

   CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_RIB1_R4_gRNA_MS2

   Depositor

   Brigitte Gasser, Diethard Mattanovich

   Article

   Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

   Inserts

   dCAS9
   MS2-VP64
   gRNA (Targeting R4 in the RIB1 promoter)
   

   Expression

   Yeast

   Promoter

   pGAP, pPOR1, and pTEF2

   Available Since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. THI VP64 pALD4 T1

   Plasmid

   #161482

   Purpose

   CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pALD4_THI11_T1_gRNA_MS2

   Depositor

   Brigitte Gasser, Diethard Mattanovich

   Article

   Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

   Inserts

   dCAS9
   MS2-VP64
   gRNA (targeting T1 of pTHI11 promoter)
   

   Expression

   Yeast

   Promoter

   pALD4, pPOR1, and pTEF2

   Available Since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. THI VP64 pALD4 T2

   Plasmid

   #161483

   Purpose

   CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pALD4_THI11_T2_gRNA_MS2

   Depositor

   Brigitte Gasser, Diethard Mattanovich

   Article

   Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

   Inserts

   dCAS9
   MS2-VP64
   gRNA (targeting T2 of pTHI11 promoter)
   

   Expression

   Yeast

   Promoter

   pALD4, pPOR1, and pTEF2

   Available Since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. THI VP64 pALD4 T3

   Plasmid

   #161484

   Purpose

   CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pALD4_THI11_T3_gRNA_MS2

   Depositor

   Brigitte Gasser, Diethard Mattanovich

   Article

   Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

   Inserts

   dCAS9
   MS2-VP64
   gRNA (targeting T3 of pTHI11 promoter)
   

   Expression

   Yeast

   Promoter

   pALD4, pPOR1, and pTEF2

   Available Since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. VanR - F165V

   Plasmid

   #158965

   Purpose

   Wild type VanR where the residue in position 165 was mutated from Phenylalanine to Valine. This mutation abolished the response to vanillic acid. The gene is under the control of the TEF1 promoter.

   Depositor

   Michael Jensen

   Article

   et al Biotechnology Notes, Volume 1, 2020, Pag

   Insert

   VanR - F165V
   

   Expression

   Yeast

   Mutation

   Wild type VanR from Caulobacter crescentus with m…

   Promoter

   TEF1

   Available Since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. VanR - F165R

    Plasmid

    #158966

    Purpose

    Wild type VanR where the residue in position 165 was mutated from Phenylalanine to Arginine. This mutation abolished the response to vanillic acid. The gene is under the control of the TEF1 promoter.

    Depositor

    Michael Jensen

    Article

    et al Biotechnology Notes, Volume 1, 2020, Pag

    Insert

    VanR - F165R
    

    Expression

    Yeast

    Mutation

    Wild type VanR from Caulobacter crescentus with m…

    Promoter

    TEF1

    Available Since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCMV Ub Lex VP16 (F442A) HA (P#1711)

    Plasmid

    #14596

    Depositor

    Matija Peterlin

    Article

    Kurosu et al Curr Biol. 2004 Jun 22. 14(12):1112-6.

    Insert

    LexA
    

    Tags

    HA, Ubiquitin, and VP16 (F442A)

    Expression

    Mammalian

    Mutation

    The VP16 sequence contains an F442A mutation. The…

    Available Since

    June 30, 2008

    Availability

    Academic Institutions and Nonprofits only

12. pEF myc Gal cycT2b (1-420) (P#1720)

    Plasmid

    #14602

    Depositor

    Matija Peterlin

    Article

    Kurosu et al Gene. 2004 Dec 8. 343(1):173-9.

    Insert

    cycT2 (CCNT2 Human)
    

    Tags

    Gal and myc

    Expression

    Mammalian

    Mutation

    amino acids 1-420 of cyclinT2

    Available Since

    June 30, 2008

    Availability

    Academic Institutions and Nonprofits only

13. pGEX cycT2b (1-589) (P#1732)

    Plasmid

    #14604

    Depositor

    Matija Peterlin

    Article

    Kurosu et al Gene. 2004 Dec 8. 343(1):173-9.

    Insert

    cycT2 (1-589) (CCNT2 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    amino acids 1-589

    Available Since

    June 30, 2008

    Availability

    Academic Institutions and Nonprofits only

14. pCMV-TM-CIBN-BLITz1-TetR-VP16

    Plasmid

    #89878

    Purpose

    expresses TM-CIBN-BLITz1-TetR-VP16 of BLITz system

    Depositor

    Hyungbae Kwon

    Article

    Lee et al Nat Methods. 2017 May;14(5):495-503. doi

    Insert

    TM-CIBN-TevN-BLITz1-TetR-VP16
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-NES-CRY2PHR-TevC

    Plasmid

    #89877

    Purpose

    expresses NES-CRY2PHR-TevC of BLITz system

    Depositor

    Hyungbae Kwon

    Article

    Lee et al Nat Methods. 2017 May;14(5):495-503. doi

    Insert

    NES-CRY2PHR-TevC
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. ampl43

    Plasmid

    #161830

    Purpose

    pRS416-dCas9-Mxi1+TetR+pRPR1(TetO)-NotI-gRNA plasmid marked with His, expressing dCas9-Mxi1 under Tef1 promoter, and a tet-inducible promoter for gRNA expression.

    Depositor

    Saeed Tavazoie

    Article

    Momen-Roknabadi et al Commun Biol. 2020 Nov 27;3(1):723. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Yeast

    Promoter

    TEF1

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFlag-CMV2-Brd4 del ET

    Plasmid

    #21938

    Depositor

    Eric Verdin

    Article

    Bisgrove et al Proc Natl Acad Sci U S A. 2007 Aug 21. 1

    Insert

    BRD4 (BRD4 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    aa 1-1362 only, ET domain deleted

    Available Since

    Sept. 16, 2009

    Availability

    Academic Institutions and Nonprofits only

18. GADD45 WT-luc

    Plasmid

    #8356

    Depositor

    Michael Greenberg

    Article

    Tran et al Science 2002 Apr 19;296(5567):530-4.

    Insert

    GADD45 promoter WT (GADD45A Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Available Since

    Dec. 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. AAV-EFS-LSL-nlsGFP-shRNA

    Plasmid

    #175584

    Purpose

    Conditional express shRNA with control of LoxP

    Depositor

    Christian Wolfrum

    Article

    Sun et al Nature. 2020 Nov;587(7832):98-102. doi:

    Type

    Empty backbone

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pWR76

    Plasmid

    #202776

    Purpose

    episomal fluorescent reporter plasmid for S. stipitis

    Depositor

    J. Brian Robertson

    Article

    Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

    Inserts

    TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
    coHPH
    ACT1 terminator (S. stipitis)
    TDH3 promoter (S. stipitis)
    coCherry
    ADH2 terminator (S. stipitis)
    ARS from S. stipitis chromosome 1
    

    Expression

    Yeast

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge