We narrowed to 838 results for: transfection

1. pSIL-eGFP-shACTN2

   Plasmid

   #52677

   Purpose

   shRNA against α-actinin-2 with eGFP transfection marker

   Depositor

   Johannes Hell

   Article

   Schnizler et al J Biol Chem. 2009 Jan 30;284(5):2697-705

   Insert

   ACTN2 (Actn2 Rat)
   

   Use

   RNAi

   Tags

   CMV-EGFP

   Promoter

   U6

   Available Since

   July 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. qTAG-C-moxGFP-Puro-H3C2

   Plasmid

   #207783

   Purpose

   Donor template for moxGFP-2A-Puro insertion into the C-terminus of the H3C2 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H3C2 Addgene #207780

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   H3C2 Homology Arms flanking a moxGFP-Puro Cassette (H3C2 Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Available Since

   Nov. 29, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pF713-pEGFP-N1-Hs-FL-METTL20 wt

   Plasmid

   #85114

   Purpose

   transfection of HeLa cell for transient expression of human full-length METTL20-EGFP fusion

   Depositor

   Pal Falnes

   Article

   Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

   Insert

   human full-length METTL20 (METTL20 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSBL_DK202

   Plasmid

   #156426

   Purpose

   Mammalian expression of 3a delta N(aa 42-275)-EGFP with CMV promoter, can be used for transfection or used to generate bacmids for baculoviral infection of mammalian cells

   Depositor

   Stephen Brohawn

   Article

   Kern et al bioRxiv [Preprint]. 2021 Jan 26:2020.06.

   Insert

   SARS-CoV-2 3a protein (aa 42-275)
   

   Tags

   EGFP-His

   Expression

   Mammalian

   Mutation

   aa 42-275

   Promoter

   CMV

   Available Since

   July 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. qTAG-N-Blast-sTagRFP-TUBA1B

   Plasmid

   #207768

   Purpose

   Donor template for Blast-2A-sTagRFP insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   TUBA1B Homology Arms flanking a Blast-sTagRFP Cassette (TUBA1B Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Available Since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. HA-IFITM3-F8-TAG-C72A

   Plasmid

   #122477

   Purpose

   expresses human IFITM3-C72A with unnatural amino acid incorporated site specifically in response to the amber codon TAG in mammalian cells when co-transfected with tRNA synthetase/tRNA pair.

   Depositor

   Howard Hang

   Article

   Spence et al Nat Chem Biol. 2019 Jan 14. pii: 10.1038

   Insert

   IFITM3 (IFITM3 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Changed Phenylalanine 8 to amber codon, cysteine …

   Available Since

   March 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV-RSV-GFP-U6-CCR5 gRNA (SpyCas9 scaffold)

   Plasmid

   #113041

   Purpose

   AAV vector; encodes GFP as well as a U6-driven CCR5-targeting gRNA (SpyCas9 scaffold)

   Depositor

   Dominik Niopek

   Article

   Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

   Insert

   gRNA CCR5 (SpCas9 scaffold), co-expressed GFP (transfection marker)
   

   Use

   AAV, CRISPR, and Synthetic Biology

   Expression

   Mammalian

   Available Since

   Nov. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. IFITM3-N21del-V93-TAG

   Plasmid

   #122480

   Purpose

   expresses human IFITM3-N21del with unnatural amino acid incorporated site specifically in response to the amber codon TAG in mammalian cells when co-transfected with tRNA synthetase/tRNA pair.

   Depositor

   Howard Hang

   Article

   Spence et al Nat Chem Biol. 2019 Jan 14. pii: 10.1038

   Insert

   IFITM3 (IFITM3 Human)
   

   Expression

   Mammalian

   Mutation

   Changed Valine 93 to amber codon and deleted amin…

   Available Since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV-RSV-GFP-U6-EMX1 gRNA (SpyCas9 scaffold)

   Plasmid

   #113040

   Purpose

   AAV vector; encodes GFP as well as a U6-driven EMX1-targeting gRNA (SpyCas9 scaffold)

   Depositor

   Dominik Niopek

   Article

   Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

   Insert

   gRNA EMX1 (SpCas9 scaffold), co-expressed GFP (transfection marker)
   

   Use

   AAV, CRISPR, and Synthetic Biology

   Expression

   Mammalian

   Available Since

   Nov. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV-RSV-GFP-U6-CFTR gRNA (SpyCas9 scaffold)

    Plasmid

    #113042

    Purpose

    AAV vector; encodes GFP as well as a U6-driven CFTR-targeting gRNA (SpyCas9 scaffold)

    Depositor

    Dominik Niopek

    Article

    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

    Insert

    CFTR gRNA (SpCas9 scaffold), co-expressed GFP (transfection marker)
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Nov. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. HA-IFITM3-F8-TAG-C105A

    Plasmid

    #122478

    Purpose

    expresses human IFITM3-C105A with unnatural amino acid incorporated site specifically in response to the amber codon TAG in mammalian cells when co-transfected with tRNA synthetase/tRNA pair.

    Depositor

    Howard Hang

    Article

    Spence et al Nat Chem Biol. 2019 Jan 14. pii: 10.1038

    Insert

    IFITM3 (IFITM3 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Changed Phenylalanine 8 to amber codon, cysteine …

    Available Since

    March 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. HA-IFITM3-V93-TAG

    Plasmid

    #122479

    Purpose

    expresses human IFITM3 with unnatural amino acid incorporated site specifically in response to the amber codon TAG in mammalian cells when co-transfected with tRNA synthetase/tRNA pair.

    Depositor

    Howard Hang

    Article

    Spence et al Nat Chem Biol. 2019 Jan 14. pii: 10.1038

    Insert

    IFITM3 (IFITM3 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Changed Valine 93 to amber codon

    Available Since

    March 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. HA-IFITM3-F8-TAG-C71A

    Plasmid

    #122476

    Purpose

    expresses human IFITM3-C71A with unnatural amino acid incorporated site specifically in response to the amber codon TAG in mammalian cells when co-transfected with tRNA synthetase/tRNA pair.

    Depositor

    Howard Hang

    Article

    Spence et al Nat Chem Biol. 2019 Jan 14. pii: 10.1038

    Insert

    IFITM3 (IFITM3 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Changed Phenylalanine 8 to amber codon, cysteine …

    Available Since

    March 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBOB-Gpr161-3xFlag

    Plasmid

    #245992

    Purpose

    Express 3xFlag C-terminal tagged mouse GPR161 transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.

    Depositor

    Zhao Zhang

    Article

    Xun et al Science. 2025 Jun 5;388(6751):eadp3989.

    Insert

    Gpr161 (Gpr161 Mouse)
    

    Use

    Lentiviral

    Tags

    3xFlag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCMV-HA-Gnai3

    Plasmid

    #245997

    Purpose

    Express HA N-terminal tagged mouse Galphai3 transiently via transfection in mammalian cells.

    Depositor

    Zhao Zhang

    Article

    Xun et al Science. 2025 Jun 5;388(6751):eadp3989.

    Insert

    Gnai3 (Gnai3 Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBOB-HA-Gna15

    Plasmid

    #246003

    Purpose

    Express HA N-terminal tagged mouse Galpha15 transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.

    Depositor

    Zhao Zhang

    Article

    Xun et al Science. 2025 Jun 5;388(6751):eadp3989.

    Insert

    Gna15 (Gna15 Mouse)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBOB-HA-Gna14

    Plasmid

    #246002

    Purpose

    Express HA N-terminal tagged mouse Galpha14 transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.

    Depositor

    Zhao Zhang

    Article

    Xun et al Science. 2025 Jun 5;388(6751):eadp3989.

    Insert

    Gna14 (Gna14 Mouse)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBOB-HA-Gnao1-A

    Plasmid

    #245998

    Purpose

    Express HA N-terminal tagged mouse GalphaoA transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.

    Depositor

    Zhao Zhang

    Article

    Xun et al Science. 2025 Jun 5;388(6751):eadp3989.

    Insert

    Gnao1 (Gnao1 Mouse)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBOB-HA-Gnaz

    Plasmid

    #246000

    Purpose

    Express HA N-terminal tagged mouse Galphaz transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.

    Depositor

    Zhao Zhang

    Article

    Xun et al Science. 2025 Jun 5;388(6751):eadp3989.

    Insert

    Gnaz (Gnaz Mouse)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBOB-HA-Gnaq

    Plasmid

    #246001

    Purpose

    Express HA N-terminal tagged mouse Galphaq transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.

    Depositor

    Zhao Zhang

    Article

    Xun et al Science. 2025 Jun 5;388(6751):eadp3989.

    Insert

    Gnaq (Gnaq Mouse)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge