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We narrowed to 14,011 results for: flag

Showing: 7771 - 7800 of 14011 results

  1. pFETCh_PBX2

    Plasmid
    #72356
    Purpose
    Donor vector for 3' FLAG tag of human PBX2
    Depositor
    Eric Mendenhall, Richard M. Myers
    Insert
    PBX2 (PBX2 Human)
    Use
    CRISPR
    Tags
    3XFLAG-P2A-NeoR
    Expression
    Mutation
    Promoter
    Available Since
    Aug. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    72356-plasmid-map-sequence-id-190163

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  4. pHEE401E

    Plasmid
    #71287
    Purpose
    Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter), Hyg resistance
    Depositor
    Qi-Jun Chen
    Article
    Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10
    Inserts
    gRNA scaffold
    zCas9
    Use
    CRISPR; Plant binary vector
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    Zea mays codon-optimized Cas9
    Promoter
    EC1.2 enhancer fused to EC1.1 promoter and U6-26p…
    Available Since
    Jan. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    71287-plasmid-map-sequence-id-352460

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  5. FH-Tet2-pEF

    Plasmid
    #41710
    Purpose
    This plasmid expresses mouse Tet2 with N-terminal FLAG and HA tags in a mammalian system
    Depositor
    Anjana Rao
    Article
    Ko et al Nature. 2010 Dec 9;468(7325):839-43. doi
    Insert
    tet methylcytosine dioxygenase 2 (Tet2 Mouse)
    Use
    Tags
    FLAG and HA
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available Since
    Feb. 18, 2014
    Availability
    Academic Institutions and Nonprofits only
    41710-plasmid-map-sequence-id-322448

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  6. CCR2-DuET

    Plasmid
    #213198
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    CCR2 (CCR2 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available Since
    May 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    213198-plasmid-map-sequence-id-431752

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  7. CNR1-DuET

    Plasmid
    #213213
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    CNR1 (CNR1 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213213-plasmid-map-sequence-id-430775

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  8. pHEE401

    Plasmid
    #71286
    Purpose
    Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter), Hyg resistance
    Depositor
    Qi-Jun Chen
    Article
    Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10
    Inserts
    sgRNA scaffold
    zCas9
    Use
    CRISPR; Plant binary vector
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    Zea mays codon-optimized Cas9
    Promoter
    EC1.2 promoter and U6-26p Arabidopsis U6 gene pro…
    Available Since
    Jan. 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    71286-plasmid-map-sequence-id-127777

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  9. CXCR3-DuET

    Plasmid
    #213220
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    CXCR3 (CXCR3 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213220-plasmid-map-sequence-id-430782

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  10. GPER-DuET

    Plasmid
    #213256
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    GPER (GPER1 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213256-plasmid-map-sequence-id-430806

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  11. pHEE2E-TRI

    Plasmid
    #71288
    Purpose
    Egg cell-specific promoter-controlled expression 3×FLAG-NLS-zCas9-NLS and two sgRNAs targeting three genes: ETC2, TRY, and CPC
    Depositor
    Qi-Jun Chen
    Article
    Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10
    Inserts
    sgRNA targeting TRY and CPC genes
    sgRNA targeting ETC2 gene
    zCas9
    Use
    CRISPR; Plant binary vector
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    Zea mays codon-optimized Cas9
    Promoter
    EC1.2 enhancer fused to EC1.1 promoter and U6-26p…
    Available Since
    Jan. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    71288-plasmid-map-sequence-id-166343

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  12. FRIG-myc-Sema4D-CD4

    Plasmid
    #51606
    Purpose
    expresses myc-tagged Sema4D/CD4 non-cleavable chimera with extracellular Sema4D, intracellular CD4 in lentiviral backbone
    Depositor
    Suzanne Paradis
    Article
    Raissi et al Mol Cell Neurosci. 2013 Nov;57:23-32. do
    Insert
    Sema4D/CD4-FLAG (Sema4d Human, Mouse)
    Use
    Lentiviral
    Tags
    FLAG and myc
    Expression
    Mammalian
    Mutation
    Sema4D amino acids 660-861 were replaced with CD4…
    Promoter
    RSV
    Available Since
    April 7, 2014
    Availability
    Academic Institutions and Nonprofits only

  13. CCR8-DuET

    Plasmid
    #213204
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    CCR8 (CCR8 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213204-plasmid-map-sequence-id-430767

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  14. CCR7-DuET

    Plasmid
    #213203
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    CCR7 (CCR7 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213203-plasmid-map-sequence-id-430766

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  15. CCR5-DuET

    Plasmid
    #213201
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    CCR5 (CCR5 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213201-plasmid-map-sequence-id-430764

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  19. CXCR4-DuET

    Plasmid
    #213221
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    CXCR4 (CXCR4 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213221-plasmid-map-sequence-id-430783

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  21. CRHR1-DuET

    Plasmid
    #213215
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    CRHR1 (CRHR1 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213215-plasmid-map-sequence-id-430777

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  22. CCR4-DuET

    Plasmid
    #213200
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    CCR4 (CCR4 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213200-plasmid-map-sequence-id-430763

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  23. LPAR1-DuET

    Plasmid
    #213329
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    LPAR1 (LPAR1 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213329-plasmid-map-sequence-id-430863

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  25. pcDNA.3.1.ADRB2-NP

    Plasmid
    #134376
    Purpose
    Nanoluc complementation assay. Expression of adrenoceptor beta 2 fused at C termimus with Natural peptide (NP) of NanoLuc. Addition of signal sequence and Flag epitope at N terminus of ADRB2.
    Depositor
    Julien Hanson
    Article
    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40
    Insert
    ADRB2-NP (ADRB2 Human)
    Use
    Tags
    Flag, natural peptide of nanoluciferase, and sign…
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    T7
    Available Since
    Aug. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    134376-plasmid-map-sequence-id-306396

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  26. pCW57.1 KBTBD4 WT

    Plasmid
    #184624
    Purpose
    Doxycycline inducible lentiviral vector for N-terminal Flag tagged KBTBD4 WT expression in mammalian cells
    Depositor
    Vincenzo D'Angiolella
    Article
    Chen et al Cell Death Differ. 2022 Apr 4. pii: 10.1
    Insert
    N-terminal Flag tagged KBTBD4 WT (KBTBD4 Human)
    Use
    Lentiviral; Doxycycline inducible
    Tags
    Expression
    Mammalian
    Mutation
    no mutation
    Promoter
    TRE promoter, Tet ON
    Available Since
    June 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    184624-plasmid-map-sequence-id-363386

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  27. pcDNA5-FRT-TO-Nprot-HF_GC3opt

    Plasmid
    #157732
    Purpose
    mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 N protein under control of a tetracycline-inducible promoter
    Depositor
    David Tollervey
    Article
    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi
    Insert
    Nprot-HF_GC3opt (N SARS-CoV-2)
    Use
    Tags
    His8-Flag
    Expression
    Mammalian
    Mutation
    codon optimized; gene expression was optimized by…
    Promoter
    Available Since
    Aug. 10, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    157732-plasmid-map-sequence-id-308146

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  28. pCAGGS-PHFF

    Plasmid
    #100280
    Purpose
    Expression vector for phycocyanobilin (PCB) synthesis in mammalian cells.
    Depositor
    Kazuhiro Aoki
    Article
    Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p
    Insert
    MTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Myc-Fd-P2A-MTS-Fnr-T7 (synthetic genes)
    Use
    Tags
    FLAG, T7 and Mitochondria-targeting sequence (MTS…
    Expression
    Mammalian
    Mutation
    All genes are codon-optimized for expression in h…
    Promoter
    CAG promoter
    Available Since
    Sept. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    100280-plasmid-map-sequence-id-194335

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  29. pU6-(BsaI)_CBh-UN-Cas9

    Plasmid
    #135011
    Purpose
    Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 with 126aa MRN-recruiting domain from HSV-1 UL12 fused to N-terminus of Cas9
    Depositor
    Yosef Shaul
    Article
    Reuven et al Biomolecules. 2019 Oct 8;9(10). pii: bio
    Insert
    humanized S. pyogenes Cas9
    Use
    Tags
    126aa domain from HSV-1 UL12 fused to the N-termi…
    Expression
    Mammalian
    Mutation
    126aa domain from HSV-1 UL12 fused to the N-termi…
    Promoter
    CBh
    Available Since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    135011-plasmid-map-sequence-id-267594

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  30. HTR1B-DuET

    Plasmid
    #213317
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    HTR1B (HTR1B Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213317-plasmid-map-sequence-id-430854

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Showing: 7771 - 7800 of 14011 results