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We narrowed to 32,367 results for: fer

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Showing: 5201 - 5220 of 32367 results

  2. Polymerase-recruited click editor - pCMV-T7-PCV2-nCas9-CC(N6) (LM2705)

    Plasmid
    #208962
    Purpose
    Polymerase-recruited click editor comprised of PCV2 fused to nCas9 with a C-terminal N6 coiled coil (CC) domain, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    PCV2-XTEN-nSpCas9-BPNLS-CC(N6)
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A)
    Promoter
    CMV and T7
    Available Since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208962-plasmid-map-sequence-id-415796

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  3. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-T7Sequenase (JO584)

    Plasmid
    #208955
    Purpose
    A variant CE1 construct with T7 Sequenase DNA polymerase, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    PCV2-XTEN-nSpCas9-BPNLS-Sequenase-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A)
    Promoter
    CMV and T7
    Available Since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208955-plasmid-map-sequence-id-415744

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  4. pAAV-hSyn-LMO3 (sbGLuc-VChR1-EYFP)

    Plasmid
    #114099
    Purpose
    fusion protein of Gaussia luciferase variant slow burn, Volvox channelrhodopsin-1, and enhanced yellow fluorescent protein for bioluminescent optogenetics
    Depositor
    Ute Hochgeschwender
    Article
    Berglund et al Proc Natl Acad Sci U S A. 2016 Jan 19;11
    Insert
    Gaussia luciferase, slow burn; Volvox Channelrhodopsin 1; EYFP
    Use
    AAV
    Expression
    Mammalian
    Promoter
    hSyn
    Available Since
    Aug. 6, 2019
    Availability
    Academic Institutions and Nonprofits only
    114099-plasmid-map-sequence-id-252568

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  8. Recruited polymerase expression - pCMV-T7-ePhi29(+exo)-CC(N5) (LM2982)

    Plasmid
    #208972
    Purpose
    Recruited ePhi29(+exo) DNA polymerase with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    ePhi29(+exo)-BPNLS-CC(N5)
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    ePhi29(+exo)(M8R/V51A/M97T/G197D/E221K/Q497P/K512…
    Promoter
    CMV and T7
    Available Since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208972-plasmid-map-sequence-id-415827

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  15. pLPC-L647RLaminA-CS

    Plasmid
    #69062
    Purpose
    encodes a lamin A mutant with Leucine 647 mutated to an Arginin and with a substitution of cysteine in CaaX-motif by a serine
    Depositor
    Gerardo Ferbeyre
    Article
    Moiseeva et al Cell Cycle. 2015 Aug 3;14(15):2408-21. d
    Insert
    LaminA (LMNA Human)
    Use
    Retroviral
    Expression
    Mammalian
    Mutation
    Lamin A with Leucine 647 mutated to Arginine and …
    Promoter
    CMV
    Available Since
    Dec. 17, 2015
    Availability
    Academic Institutions and Nonprofits only

  18. ss-ED-mCherry-GPI

    Plasmid
    #163680
    Purpose
    Exprsses the GPI-anchored luminal domain of ST6GAL1 with a mCherry tag in mammalian cells
    Depositor
    Lei Lu
    Article
    Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27
    Insert
    The luminal domain of ST6 beta-galactoside alpha-2,6-sialyltransferase 1 with N-terminally tagged signal sequences (ST6GAL1 Human)
    Tags
    GPI anchor, mCherry, and signal sequences
    Expression
    Mammalian
    Mutation
    This chimera contains the ecto-domain of ST. Its …
    Available Since
    Nov. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    163680-plasmid-map-sequence-id-322820

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  20. pAAV_hSyn_HA-TALE(NG)-Tet1FL-NLS_2A_EGFP_W3SL

    Plasmid
    #172195
    Purpose
    AAV sequence-specific control vector for TALE-Tet1FL-SID4X. Synapsin promoter for neuronal expression. C-terminal, self-cleaving EGFP marker.
    Depositor
    Garrett Kaas
    Article
    Greer et al J Neurosci. 2021 Jan 27;41(4):578-593. d
    Insert
    HA-TALE(NG)-Tet1FL-NLS_2A_EGFP_W3SL
    Use
    AAV and Mouse Targeting; Tale
    Tags
    HA, NLS, and T2A-EGFP
    Expression
    Mammalian
    Mutation
    transcription regulatory domain removed
    Promoter
    hSyn human synapsin (mammalian neuronal expressio…
    Available Since
    Aug. 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    172195-plasmid-map-sequence-id-343564

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Showing: 5201 - 5220 of 32367 results