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gRNA-eGFP-Reporter

Plasmid

#60719

Purpose

Expresses guide RNA used to activate pGL3-Basic-8x-gRNA-eGFP reporter.

Depositor

Charles Gersbach

Publication

Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

gRNA (5'-AAAGGTCGAGAAACTGCAAA-3')

Use

CRISPR

Expression

Mammalian

Promoter

U6 and mouse U6

Available since

Jan. 30, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pDS257

Plasmid

#34971

Purpose

Expresses Mid2(SS/TM), containing only the signal sequence and transmembrane helix, fused to GFP and LOVpep.

Depositor

Michael Glotzer

Publication

Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

Insert

Mid2(SS/TM)-GFP-LOVpep

Expression

Yeast

Available since

March 23, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pEntr-GATA4flbio

Plasmid

#32937

Depositor

William Pu

Publication

He et al Proc Natl Acad Sci U S A. 2011 Apr 5. 10

Insert

GATA4 (Gata4 Mouse)

Use

Gateway Cloning

Tags

flag-bio

Promoter

none

Available since

Jan. 10, 2012

Availability

Academic Institutions and Nonprofits only

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pOPINJ

Plasmid

#26045

Depositor

Ray Owens

Publication

Berrow et al Nucleic Acids Res. 2007. 35(6):e45.

Insert

HisGST3C-lacZ

Tags

MAHHHHHH_GST_LEVLFQGT

Expression

Bacterial, Insect, and Mamm…

Available since

Oct. 19, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pLKO.1-GSK3β-#2

Plasmid

#32497

Depositor

Alex Toker

Publication

Yoeli-Lerner et al Mol Cancer Res. 2009 Mar;7(3):425-32. Ep

Insert

GSK3B (GSK3B Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6

Available since

Sept. 26, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pGEX-4T2-Rheb

Plasmid

#15889

Depositor

John Blenis

Publication

Tee et al Curr Biol. 2003 Aug 5. 13(15):1259-68.

Insert

Rheb (RHEB Human)

Tags

GST

Expression

Bacterial

Available since

Oct. 26, 2007

Availability

Academic Institutions and Nonprofits only
pOPINK-Cezanne (OTU, aa 53-446)

Plasmid

#61581

Purpose

Expresses human Cezanne (OTU domain) in E. coli.

Depositor

David Komander

Publication

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

Cezanne (OTUD7B Human)

Tags

His6-GST-3C

Expression

Bacterial

Mutation

Deleted aa 1-52 and aa 447-843.

Promoter

T7

Available since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pET21a-SerRS-His

Plasmid

#124118

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Publication

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Seryl-tRNA ligase (serS Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET21a-LysRS-His

Plasmid

#124114

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Publication

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Lysine-tRNA ligase (lysU Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pEBB HA RelA

Plasmid

#74892

Purpose

Express RelA in mammalian cells

Depositor

Ezra Burstein, Colin Duckett

Publication

Burstein et al J Biol Chem. 2005 Jun 10;280(23):22222-3

Insert

RelA (RELA Human)

Tags

HA

Expression

Mammalian

Promoter

EF-1alpha promoter

Available since

June 23, 2016

Availability

Academic Institutions and Nonprofits only

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pCS2-Gal4ff.zf1

Plasmid

#61392

Purpose

mRNA synthesis of Gal4ff codon modified for zebrafish

Depositor

Harold Burgess

Publication

Horstick et al Nucleic Acids Res. 2015 Jan 27. pii: gkv

Insert

Gal4ff.zf1

Use

Zebrafish expression

Promoter

sp6

Available since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pGEX-2T-IQGAP1

Plasmid

#30107

Depositor

David Sacks

Publication

Ho et al J Biol Chem. 1999 Jan 1. 274(1):464-70.

Insert

IQGAP1 (IQGAP1 Human)

Tags

GST

Expression

Bacterial

Available since

July 29, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pBabe WT Akt2

Plasmid

#14551

Depositor

Joan Brugge

Publication

Irie et al J Cell Biol. 2005 Dec 19. 171(6):1023-34

Insert

Akt2 (AKT2 Human)

Use

Retroviral

Tags

HA tag

Expression

Mammalian

Available since

April 12, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pBabe-TetCMV-puro-mCherry-PA-Rac1

Plasmid

#22035

Purpose

Expression of photoactivatable Rac1

Depositor

Klaus Hahn

Publication

Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

Insert

mCherry-PA-Rac1 (RAC1 Avena sativa (oat), Human)

Use

Retroviral

Expression

Mammalian

Mutation

Rac1 starts at I4 and contains mutations Q61L, E9…

Promoter

Tet-CMV

Available since

Sept. 11, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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ADORA3-Tango

Plasmid

#66212

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Publication

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ADORA3 (ADORA3 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-NOD2-EGFP

Plasmid

#131207

Purpose

mammalian expression, tet R

Depositor

Thomas Kufer

Publication

Pei et al EMBO J. 2021 Jul 1;40(13):e106272. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

NOD2 (NOD2 Human)

Tags

EGFP

Expression

Mammalian

Promoter

pCMV

Available since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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Ai34

Plasmid

#34881

Depositor

Hongkui Zeng

Publication

Madisen et al Nat Neurosci. 2012 Mar 25;15(5):793-802.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CAG-Floxed Synaptophysin-tdTomato

Use

Mouse Targeting

Tags

tdTomato

Promoter

CAG

Available since

March 1, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pHAGE-CMV-Rheb(S16H)-IRES-eGFP-W

Plasmid

#32520

Depositor

Mustafa Sahin

Publication

Nie et al Nat Neurosci. 2010 Feb;13(2):163-72. Epu

Insert

Constitutively active Rheb (S16H) (Rheb Rat)

Use

Lentiviral

Expression

Mammalian

Mutation

S16H to make constitutively active

Promoter

CMV

Available since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pEGFPC1-muscle Amphiphysin 2

Plasmid

#22213

Depositor

Pietro De Camilli

Publication

Butler et al J Cell Biol. 1997 Jun 16. 137(6):1355-67

Insert

Amphiphysin II (BIN1 Human)

Tags

GFP

Expression

Bacterial and Mammalian

Available since

Oct. 9, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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Flag pLJM1 Rap2A

Plasmid

#19311

Depositor

David Sabatini

Publication

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

RAP2A, member of RAS oncogene family (RAP2A Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Available since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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