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pCI-HA-ERK5-FL

Plasmid

#31817

Depositor

Astar Winoto

Article

Kasler et al Mol Cell Biol. 2000 Nov;20(22):8382-9.

Insert

Erk5 (Mapk7 Mouse)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2011

Availability

Academic Institutions and Nonprofits only
proE-cad670-Luc-mEbox

Plasmid

#42084

Depositor

Kumiko Ui-Tei

Article

Mazda et al PLoS One. 2011;6(12):e28688. doi: 10.137

Insert

E-cadherin (CDH1 Human)

Use

Luciferase

Expression

Mammalian

Mutation

contains E-cadherin promoter region from -670 to …

Promoter

E-cadherin

Available Since

April 10, 2013

Availability

Academic Institutions and Nonprofits only

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pJH200

Plasmid

#13327

Depositor

Martin Gellert

Article

Lieber et al Genes Dev. 1987 Oct . 1(8):751-61.

Insert

recombination substrate

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-FRB-LgBiT

Plasmid

#182650

Purpose

FRB-LgBiT

Depositor

Masaharu Somiya

Article

Somiya et al Mol Pharm. 2022 May 20. doi: 10.1021/acs

Insert

FRB-LgBiT fusion

Tags

LgBiT

Expression

Mammalian

Promoter

CMV

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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pLEX-EF1a ANXA11-mEmerald

Plasmid

#164210

Purpose

pLEX lentivirus backbone expresses mEmerald tagged ANXA11 under EF1a promoter

Depositor

Jennifer Lippincott-Schwartz

Article

Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

Insert

ANXA11 (ANXA11 Human)

Use

Lentiviral

Tags

mEmerald

Promoter

EF1a

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-pMecp2-dSaCas9-KRAB-pU6-sgRNA

Plasmid

#158988

Purpose

Vector E encodes pAAV-pMecp2-dSaCas9-KRAB-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of CRISPR interference in neurons

Depositor

Chung Tin

Article

Lau et al Mol Ther Nucleic Acids. 2019 Jun 7;16:63

Insert

dSaCas9-KRAB

Use

AAV and CRISPR

Expression

Mammalian

Promoter

pMecp2

Available Since

Aug. 29, 2020

Availability

Academic Institutions and Nonprofits only

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MAC_N_MED13

Plasmid

#111689

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

Insert

MED13 (MED13 Human)

Expression

Mammalian

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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phage UbiC scAB-GFP

Plasmid

#104998

Purpose

Lentiviral vector expressing sequence-optimized single chain Antibody-GFP fusion protein for SunTag labeling

Depositor

Jeffrey Chao

Article

Voigt et al Cell Rep. 2017 Dec 26;21(13):3740-3753.

Insert

single chain Antibody against GCN4

Use

Lentiviral

Tags

GB1, HA tag, and sfGFP

Expression

Bacterial and Mammalian

Promoter

UbiC

Available Since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

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GFP-CECR2

Plasmid

#65385

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

CECR2 (CECR2 Human)

Tags

EmGFP

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
pAC84-pCR8-dCas9

Plasmid

#48218

Purpose

dCas9 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression

Depositor

Rudolf Jaenisch

Article

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Insert

dCas9(D10A;H840A)

Use

CRISPR and Gateway Cloning

Tags

HA Tag

Mutation

D10A;H840A

Available Since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3-GI-ZFP2

Plasmid

#42215

Purpose

Expresses GI-ZFP2 for light-inducible activation of gene expression

Depositor

Charles Gersbach

Article

Polstein et al J Am Chem Soc. 2012 Oct 10;134(40):16480

Insert

GIGANTEA-FLAG-ZFP2 (GI Synthetic, Mustard Weed)

Tags

FLAG

Promoter

CMV

Available Since

Jan. 29, 2013

Availability

Academic Institutions and Nonprofits only

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pESC-URA::CYP71AV1/CPR

Plasmid

#20118

Depositor

Jay Keasling

Article

Ro et al Nature. 2006 Apr 13. 440(7086):940-3.

Insert

amorphadiene oxidase and p450 reductase

Use

Synthetic Biology

Expression

Yeast

Available Since

March 2, 2009

Availability

Academic Institutions and Nonprofits only
pYPQ146

Plasmid

#69298

Purpose

Golden Gate recipient and Gateway entry vector; assembly of 6 gRNAs

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available Since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pLentiRNAGuide_003

Plasmid

#192505

Purpose

For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat and smRNA, evopreQ1, capture sequences and GFP

Depositor

Neville Sanjana

Article

Wessels et al Nat Methods. 2023 Jan;20(1):86-94. doi:

Insert

hU6-RfxCas13d-DR1-BsmBI-DR-smRNA-SapI-CS1-evopreQ1-EFS-EGFP-2A-Puro-WPRE

Use

Lentiviral

Promoter

hU6

Available Since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pETcon_SARS-CoV-2_RBD

Plasmid

#166782

Purpose

Yeast surface display vector, expressing the SARS-2 RBD, 201aa construct

Depositor

Jesse Bloom

Article

Starr et al Cell. 2020 Sep 3;182(5):1295-1310.e20. d

Insert

Spike receptor binding domain (RBD) from SARS-CoV-2 (S )

Expression

Yeast

Available Since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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Rad51–Cas9(D10A)

Plasmid

#125567

Purpose

Expresses the Rad51–Cas9(D10A) fusion construct in mammalian cells under CMV promoter

Depositor

David Liu

Article

Rees et al Nat Commun. 2019 May 17;10(1):2212. doi:

Insert

Rad51–Cas9(D10A) (RAD51 Human)

Use

CRISPR

Expression

Mammalian

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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psigma1-EGFP

Plasmid

#53611

Purpose

Expresses sigma1 of adaptor protein of AP1

Depositor

Tom Kirchhausen

Article

Kural et al Cell Rep. 2012 Nov 29;2(5):1111-9. doi:

Insert

sigma 1 (Ap1s1 Mouse)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

July 23, 2014

Availability

Academic Institutions and Nonprofits only

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TCF4-DN in pLX303

Plasmid

#42592

Depositor

William Hahn

Article

Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

Insert

TCF7L2 (TCF7L2 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

31 first amino acids of hTcf4 have been deleted

Promoter

CMV

Available Since

Feb. 8, 2013

Availability

Academic Institutions and Nonprofits only
pEGFPC1-GluR1Ctail

Plasmid

#32441

Depositor

Robert Malinow

Article

Rumpel et al Science. 2005 Apr 1;308(5718):83-8. Epub

Insert

GluR1 (GRIA1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

Contains the last C-terminal 81 amino acids of th…

Promoter

CMV

Available Since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC2A1

Plasmid

#132103

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC2A1 (SLC2A1 Human)

Expression

Mammalian

Available Since

Dec. 3, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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