We narrowed to 29,763 results for: REP
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Plasmid#184380PurposeThe mouse Myod1 gene is expressed under a doxcycline-inducible TRE3G promoterDepositorAvailable SinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only
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pMALp2x-SCO5461(E164D)(43-204)
Plasmid#42508DepositorInsertguanosine ADP-ribosyltransferase (SCO5461 Streptomyces coelicolor A3(2))
UseTagsmaltose-binding proteinExpressionBacterialMutationDeleted amino acids 1-42 (nucleotide 1-126); chan…PromotertacAvailable SinceApril 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCVL.SSA TLR (Ani)
Plasmid#45577PurposeCodes for the SSA-TLR with the target site for I-Ani I in a lentiviral backboneDepositorInserts5' iRFP arm
eGFP with I-Ani I TS
+3 mCherry
3' iRFP arm
UseLentiviralTagsExpressionBacterial and MammalianMutationembedded I-Ani I TS from 163-185bp, truncated 25 …PromoterSFFVAvailable SinceNov. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
FUGW-RUSH-LAMP1-V5-APEX2
Plasmid#228524PurposeLentiviral vector, expresses Strep-KDEL-IRES-SBP-(Rat)LAMP1-V5-APEX2 in mammalian cells.DepositorInsertLAMP1 (Lamp1 Rat)
UseLentiviralTagsStreptavidin Binding Protein (SBP), Streptavidin-…ExpressionMammalianMutationPromoterUbcAvailable SinceFeb. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
FUGW-RUSH-LAMP1-mScarleti
Plasmid#228526PurposeLentiviral vector, expresses Strep-KDEL-IRES-SBP-(Rat)LAMP1-mScarleti in mammalian cells.DepositorInsertLAMP1 (Lamp1 Rat)
UseLentiviralTagsStreptavidin Binding Protein (SBP), Streptavidin-…ExpressionMammalianMutationPromoterUbcAvailable SinceFeb. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
10279-M30-663
Plasmid#225663PurposeLentivirus protein expression. Matched control construct for 8522-M31-663 (Addgene # 225661) gives basal expression from mCMVp for gating/thresholdingDepositorInsertdsCherry
UseLentiviralTagsExpressionMutationPromotermCMVpAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET3a.H2B-K120C
Plasmid#224706PurposeExpresses Human H2B K120C mutant in bacterial cells for fluorescent labelling of histone octamersDepositorAvailable SinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRAR104
Plasmid#185034Purpose400 nt l31 promoter region, l31 5'UTR A74 and A76 deletion, 90 nt of l31 coding region, glycine linker, sfGFP, trrnBDepositorInsertL31-sfGFP
UseTagsExpressionBacterialMutationA74 and A76 deletion of rpmE 5'UTR, Only fir…PromoterAvailable SinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRAR105
Plasmid#185035Purpose400 nt l31 promoter region, l31 5'UTR G52 and G79 deletion, 90 nt of l31 coding region, glycine linker, sfGFP, trrnBDepositorInsertL31-sfGFP
UseTagsExpressionBacterialMutationG52 and G79 deletion of rpmE 5'UTR, Only fir…PromoterAvailable SinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRAR107
Plasmid#185037Purpose400 nt l31 promoter region, l31 5'UTR AGA74-76GAG mutation, 90 nt of l31 coding region, glycine linker, sfGFP, trrnBDepositorInsertL31-sfGFP
UseTagsExpressionBacterialMutationAGA74-76GAG mutation of rpmE 5'UTR, Only fir…PromoterAvailable SinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRAR109
Plasmid#185038Purpose400 nt l31 promoter region, l31 5'UTR G60 and C69 deletion, 90 nt of l31 coding region, glycine linker, sfGFP, trrnBDepositorInsertL31-sfGFP
UseTagsExpressionBacterialMutationG60 and C69 deletion of rpmE 5'UTR, Only fir…PromoterAvailable SinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRAR110
Plasmid#185039Purpose400 nt l31 promoter region, l31 5'UTR G62 and G67 deletion, 90 nt of l31 coding region, glycine linker, sfGFP, trrnBDepositorInsertL31-sfGFP
UseTagsExpressionBacterialMutationG62 and G67 deletion of rpmE 5'UTR, Only fir…PromoterAvailable SinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
GFP-Bcl-2-K17R-K22R
Plasmid#220294Purposemammalian expression of Bcl-2-K17R-K22R tagged with GFPDepositorAvailable SinceJune 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
GFP-Bcl-2-G128L-A131L-T132L
Plasmid#220304Purposemammalian expression of Bcl-2-G128L-A131L-T132L tagged with GFPDepositorInsertBcl2 (BCL2 Human)
UseTagsEmerald GFPExpressionMammalianMutationG128L-A131L-T132LPromoterCMVAvailable SinceJune 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDonor-PRF1-T2A-cDNA
Plasmid#209076PurposeTargeting vector for the human PRF1 locus to replace exon 3 with repaired cDNADepositorAvailable SinceMarch 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCENPBR pc861
Plasmid#167569PurposeHuman CENP-B (aa 1-169 containing DNA-binding domain) cloned into pDsRed1-N1 vector (Clontech).DepositorInsertHomo sapiens centromere protein B (CENPB Human)
UseTagsDs-RedExpressionMammalianMutationPromoterCMVAvailable SinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMeCP2miRFP670 pc3942
Plasmid#167568PurposeThe miRFP670-gene is ligated c-terminally to the rat meCP2-gene.DepositorAvailable SinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
TetO-FUW-FLAG-NLS-EnR-MCS_N-term
Plasmid#136884PurposeDoxycycline-inducible vector backbone containing the engrailed (EnR) repressor domain with FLAG and NLS sequences, followed by a multiple cloning site (MCS)DepositorInsertengrailed repressor domain (en Fly)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterAvailable SinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
TetO-FUW-MCS-EnR_C-term
Plasmid#136885PurposeDoxycycline-inducible vector backbone containing a multiple cloning site (MCS), the engrailed (EnR) repressor domain and a STOP codonDepositorInsertengrailed repressor domain (en Fly)
UseLentiviralTagsNoneExpressionMammalianMutationPromoterAvailable SinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
lenti-EF1a-dCas9-KRAB-Puro
Plasmid#99372Purpose3rd generation lenti vector encoding dCas9-KRAB with 2A puromycin resistance marker (EF1a-dCas9-KRAB-T2A-Puro-WPRE)DepositorInsertdCas9-KRAB-T2A-Puro
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF-1aAvailable SinceAug. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 iCasper T2A HO1
Plasmid#64278PurposeExpresses infrared fluorescent executioner caspase reporter (iCasper) plus human heme oxygenase 1DepositorInsertInfrared fluorescent executioner caspase reporter (iCasper) T2A heme oxygenase-1: (HMOX1 Synthetic)
UseTagsExpressionMammalianMutationPromoterCMVAvailable SinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
kiCAP-AAV-BI-hTFR1
Plasmid#218796PurposeRepCap for AAV productionDepositorInsertAAV Rep-Cap BI-hTFR1
UseTagsExpressionMammalianMutationPromoterAvailable SinceMay 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
lentiGuide-Hygro-dTomato
Plasmid#99376PurposeExpresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A dTomato from EF-1a promoter. 3rd generation lentiviral backbone.DepositorInsertsS. pyogenes sgRNA cassette
Hygro-P2A-dTomato
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF-1a and hU6Available SinceAug. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pU6-(BbsI)_CBh-Cas9-T2A-mCherry
Plasmid#64324PurposeExpression vector for sgRNAs cloned into the BbsI sites and for expression of Cas9 linked to mCherry via a T2A peptideDepositorInsertsCas9
sgRNA cassette
UseCRISPRTags3xFLAG, NLS, and T2A-mCherryExpressionMammalianMutationPromoterCBh and U6Available SinceSept. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCF140_Gag-SpyCas9-NLS
Plasmid#225959PurposeCMV-Intron-Gag-SpyCas9-NLS. Expresses Gag-SpyCas9-NLS for VLP production.DepositorInsertCMV-Intron-Gag-SpyCas9-NLS
UseTagsExpressionMammalianMutationPromoterAvailable SinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
lentiGuide-Hygro-mTagBFP2
Plasmid#99374PurposeExpresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A mTagBFP2 from EF-1a promoter. 3rd generation lentiviral backbone.DepositorInsertsS. pyogenes sgRNA cassette
Hygro-P2A-mTagBFP2
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF-1a and hU6Available SinceAug. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pIA123
Plasmid#234038PurposeE. coli-C. difficile shuttle vector for CRISPR editing in C. difficile. Pxyl::Cas9-opt Pgdh::sgRNA-cdr_0985. PstI site to add DNA for homology directed repair. MscI and NotI for replacement of sgRNA.DepositorInsertsCas9
sgRNA
UseCRISPRTagsExpressionBacterialMutationPromoterPgdh and PxylAvailable SinceMarch 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pME-Luciferase-P2A-H2A-mCherry (JDW 926)
Plasmid#224488PurposeGateway compatible middle entry clone containing a Luciferase P2A H2A-mCherry dual reporter (Luciferase/nuclear red fluorescent reporter)DepositorInsertLuciferase-P2A-H2A-mCherry
UseLuciferaseTagsExpressionMutationPromoterAvailable SinceSept. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
lenti-EF1a-dCas9-VP64-Puro
Plasmid#99371Purpose3rd generation lenti vector encoding dCas9-VP64 with 2A puromycin resistance marker (EF1a-dCas9-VP64-T2A-Puro-WPRE)DepositorInsertdCas9-VP64-T2A-Puro
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF-1aAvailable SinceAug. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
lentiGuide-Hygro-eGFP
Plasmid#99375PurposeExpresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A eGFP from EF-1a promoter. 3rd generation lentiviral backbone.DepositorInsertsS. pyogenes sgRNA cassette
Hygro-P2A-eGFP
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF-1a and hU6Available SinceAug. 18, 2017AvailabilityAcademic Institutions and Nonprofits only