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We narrowed to 13,953 results for: LEA

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Showing: 5261 - 5280 of 13953 results

  5. 2xMARS-nGFP

    Plasmid
    #205235
    Purpose
    Expresses two repeats of PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody in mammalian cells
    Depositor
    Jeremy Baskin
    Publication
    Cao et al Nat Cell Biol. 2024 Oct;26(10):1804-1816
    Insert
    2xPLEKHA5(143-271)-K163A/R164A-HA-nGFP (PLEKHA5 Human)
    Tags
    HA, Nuclear Export Sequence, mScarlet-i, and nGFP…
    Expression
    Mammalian
    Mutation
    amino acids 143-271 of PLEKHA5 (GenBank reference…
    Promoter
    CMV
    Available since
    July 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    205235-plasmid-map-sequence-id-404746

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  6. pAAV2-CAG-3xNLS-AausFP1

    Plasmid
    #191096
    Purpose
    To express a bright green FP to label eucaryotic cell nuclei. To be used in tissue-clearing methods and other fluorescent microscopy methods
    Depositor
    Murray Blackmore, Pantelis Tsoulfas
    Publication
    Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:
    Insert
    3xNLS-AausFP1
    Use
    AAV and Synthetic Biology
    Tags
    Three repeats of the nuclear localizzation seque…
    Expression
    Mammalian
    Mutation
    Optimized to Human codon usage
    Promoter
    CMV enhancer and CAG
    Available since
    Dec. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    191096-plasmid-map-sequence-id-380123

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  8. 6xHis-TEV-mCherry-LC3B-Gly (Microtubule-associated proteins 1A/1B light chain 3B)

    Plasmid
    #169168
    Purpose
    Conjugatable form of LC3B lacking 5 C-terminal amino acids (MKLSV), with C-terminal Glycine120 exposed for lipidation reaction.
    Depositor
    Sascha Martens
    Publication
    Zaffagnini et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017
    Insert
    MAP1LC3B (MAP1LC3B Human)
    Tags
    6X Histidine Tag, TEV cleavage site, mCherry
    Expression
    Bacterial
    Promoter
    T7 lac promoter
    Available since
    May 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    169168-plasmid-map-sequence-id-332048

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  10. XClone-Cas9-Cronus-T2A-BFP

    Plasmid
    #141209
    Purpose
    Tunable and temporal expression control of Cas9
    Depositor
    Rory Johnson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cas9
    Expression
    Mammalian
    Available since
    July 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    141209-plasmid-map-sequence-id-299562

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  11. pGBW-m4136463

    Plasmid
    #149527
    Purpose
    Bacterial expression plasmid for SARS-CoV-2 E (envelope)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 E (envelope) (E Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    6xHIS;Linker_GGSG;SUMO tag;Linker_GGSG;Cleavable …
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    May 14, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149527-plasmid-map-sequence-id-300219

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  12. pelB-MBP-P66-ss

    Plasmid
    #137045
    Purpose
    E. coli expression clone (T7lac promoter) for mature P66 (aa 22-618) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage
    Depositor
    Debra Hansen
    Publication
    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10
    Insert
    AAC66949.1 (p66 Borrelia burgdorferi B31)
    Tags
    PelB signal peptide + His10 + maltose binding pro…
    Expression
    Bacterial
    Mutation
    mature P66: lacks the P66 signal peptide
    Promoter
    T7lac
    Available since
    April 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    137045-plasmid-map-sequence-id-291951

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  15. pCEP4-gp120(BaL)-CD4

    Plasmid
    #98957
    Purpose
    Mammalian expression plasmid for CD4-fused gp120 from HIV-1 BaL isolate
    Depositor
    Erik Procko
    Publication
    Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HIV-1 (BaL) gp120
    Tags
    CD4 (domains D1-D2), CD5 leader sequence, FLAG ep…
    Expression
    Mammalian
    Mutation
    Codon-optimized synthetic gene
    Promoter
    CMV
    Available since
    April 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    98957-plasmid-map-sequence-id-193406

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  17. pSc1

    Plasmid
    #80436
    Purpose
    Expresses eGFP along with an U6 promoter driven gRNA which cleaves the plasmid in-cell
    Depositor
    Ervin Welker
    Publication
    Talas et al DNA Res. 2017 Jun 30. doi: 10.1093/dnare
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    sp Cas9 gRNA
    eGFP
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CMV and U6
    Available since
    Aug. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    80436-plasmid-map-sequence-id-151445

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  19. pcDNA4-hB90

    Plasmid
    #18968
    Depositor
    Mariano Viapiano
    Publication
    Hu et al J Biol Chem. 2008 Sep 5;283(36):24848-59
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Brevican C-terminus (BCAN Human)
    Expression
    Mammalian
    Mutation
    Human brevican signal peptide aa 1-23 followed by…
    Available since
    Dec. 12, 2008
    Availability
    Academic Institutions and Nonprofits only

  20. pGD251

    Plasmid
    #132372
    Purpose
    contains the sequence of mCerulean3 for C-term fusions in the pGGD000 backbone (GreenGate)
    Depositor
    Rüdiger Simon
    Publication
    Denay et al Plant Direct. 2019 Dec 6;3(12):e00189. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    linker-mCerulean3
    Use
    Greengate entry plasmid
    Available since
    Oct. 31, 2019
    Availability
    Academic Institutions and Nonprofits only
    132372-plasmid-map-sequence-id-258710

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Showing: 5261 - 5280 of 13953 results