161,727 results

1. pSF4 TetCMV ATF4(5'UTR)-SunTag-Renilla-FKBP-Stop-24xMS2v5-CTE-polyA

   Plasmid

   #164615

   Purpose

   Expresses ATF4(5'UTR)-SunTag-Renilla mRNA reporter under tetracycline-inducible promoter. SunTag and Renilla luciferase are in frame with the main start codon of ATF4.

   Depositor

   Jeffrey Chao

   Article

   Mateju et al Cell. 2020 Dec 23;183(7):1801-1812.e13.

   Insert

   Renilla luciferase
   

   Use

   Tags

   24xMS2v5 (3'UTR) and SunTag

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   TetCMV

   Available since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. mito-mStayGold_pTwistCMVBetaGlobWPRENeo

   Plasmid

   #221898

   Purpose

   Expresses mStayGold targeted to the mitochondria

   Depositor

   Janelia Integrative Imaging

   Insert

   cox8-mStayGold
   

   Use

   Tags

   Cox8

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pYPQAT05

   Plasmid

   #223377

   Purpose

   T-DNA vector for SpCas9 mediated mutagenesis for monocot plants; NGG PAM; Cas9 was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; BASTA for plants selection.

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

   Insert

   ZmUbi-SpCas9-OsU3-gRNA scaffold
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDEST-CMV-N-mCherry

   Plasmid

   #123215

   Purpose

   N-terminal mCherry tag. Gateway destination vector for mammalian expression.

   Depositor

   Robin Ketteler

   Article

   Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFP-TDP-43

   Plasmid

   #235487

   Purpose

   Expression of human TDP-43-EGFP

   Depositor

   Maxime Rousseaux

   Article

   Suk et al Mol Neurodegener. 2025 Mar 28;20(1):38.

   Insert

   TARDBP (TARDBP Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 30, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pAAV-EF1α-F-FLEX-mNaChBacMut-T2A-tdTomato

   Plasmid

   #60660

   Purpose

   Flp-dependent expression of mNaChBacMut-T2A-tdTomato

   Depositor

   Massimo Scanziani

   Article

   Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

   Insert

   FLEX-mNaChBacMut-T2A-tdTomato
   

   Use

   AAV

   Tags

   Expression

   Mutation

   E191K

   Promoter

   EF1α

   Available since

   Oct. 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR221_SLC7A8

   Plasmid

   #132233

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC7A8 (SLC7A8 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 8, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. 35S-sfGFP-nosT

   Plasmid

   #80129

   Purpose

   Transient expression vector for sfGFP in plants

   Depositor

   Yutaka Kodama

   Article

   Fujii et al Plant Biotech. 2015; 32(1): 81-87.

   Type

   Empty backbone

   Use

   Tags

   sfGFP

   Expression

   Plant

   Mutation

   Promoter

   CaMV35S

   Available since

   Aug. 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBEN276

   Plasmid

   #69150

   Purpose

   Integration of luciferase operon in Tn7 insertion site of Gram negative bacteria

   Depositor

   Pierre Germon

   Article

   Howe et al BMC Microbiol. 2010 Jul 23;10:197. doi:

   Insert

   luxCDABE
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   frr

   Available since

   Dec. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pmCherry-SopF

    Plasmid

    #135174

    Purpose

    Expresses an mCherry-SopF fusion in mammalian cells

    Depositor

    Leigh Knodler

    Article

    Lau et al PLoS Pathog. 2019 Jul 24;15(7):e1007959.

    Insert

    SopF (STM1239 Salmonella enterica serovar Typhimurium)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAP215.rAAV.mU6-sgRNA.Handle.EF1a-mTagBFP2

    Plasmid

    #217635

    Purpose

    AAV backbone for sgRNA expression. Contains a Lox-flanked handle sequence for Cre-dependent PCR amplification of sgRNAs. Protospacer is cloned between BstXI and BlpI.

    Depositor

    Martin Kampmann

    Insert

    mU6-sgRNA, Handle sequence, EF1a-mTagBFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF1alpha and mU6

    Available since

    Oct. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti-TRE-GFP-Blast

    Plasmid

    #135673

    Purpose

    Dox-inducible expression (TRE promoter) of GFP cDNA (control for other cDNAs), Blasticidin selection

    Depositor

    Florian Karreth

    Insert

    GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. phU1-PspCas13b-non-targeting (pEZ36)

    Plasmid

    #229053

    Purpose

    Cloning backbone for PspCas13b crRNA (Human U1 promoter)

    Depositor

    S Chul Kwon

    Article

    Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBAD-iPASnFR-L

    Plasmid

    #225238

    Purpose

    Bacterial expression of iPASnFR-L

    Depositor

    Loren Looger

    Article

    Tamura et al bioRxiv 2024.08.21.609037

    Insert

    iPASnFR-L
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti-mCherry-Cre-blast

    Plasmid

    #179390

    Purpose

    Lentiviral expression of Cre recombinase and mCherry reporter from CMV promoter (separated by P2A peptide), and blasticidin resistance gene for selection in mammalian cells.

    Depositor

    Moshe Szyf

    Article

    Sapozhnikov et al Nat Commun. 2021 Sep 29;12(1):5711. doi:

    Insert

    mCherry (P2A) Cre recombinase
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCis mCherry-SS Himar1 A7

    Plasmid

    #84687

    Purpose

    Can be used for the transformation of rickettsia, anaplasma and ehrlichia. Uses the Himar1 transposon system, with the A7 allele and both transposase and transposon contained in one plasmid.

    Depositor

    Ulrike Munderloh

    Article

    Munderloh et al Intracellular Pathogens II: Rickettsiale

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pXG237

    Plasmid

    #141281

    Purpose

    ATF4 translational reporter

    Depositor

    Martin Kampmann

    Article

    Guo et al Nature. 2020 Mar;579(7799):427-432. doi:

    Insert

    uORF1/2 of ATF4 followed by mApple
    

    Use

    Lentiviral

    Tags

    mApple

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHAGE2-TetOminiCMV-SV40LT

    Plasmid

    #136616

    Purpose

    Dox-inducible lentiviral vector expressing SV40 large T antigen for immortalizing cells

    Depositor

    Hans Schöler

    Article

    Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

    Insert

    SV40 large T antigen
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. DiLiCre 2.0

    Plasmid

    #198752

    Purpose

    A Cre recombinase that is expressed upon doxycycline when combined with the UbC-blasticidine-P2A-TETon-3G transactivator. DiLiCre 2.0 is a cre recombinase that is activated by violet light.

    Depositor

    Jacco van Rheenen

    Article

    Vizoso et al Nat Commun. 2022 Oct 28;13(1):6442. doi:

    Insert

    Cre-PhoCI-ERT2-ERT2
    

    Use

    Lentiviral

    Tags

    Cre, kept in a photocleavable site, followed by 2…

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    TRE3GV

    Available since

    July 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET-29b(+)_His-CBD-SpEndoH

    Plasmid

    #218196

    Purpose

    Bacterial expression of a His-tagged Chitin Binding fusion of Streptomyces plicatus EndoH

    Depositor

    Florence Pojer

    Insert

    EndoH
    

    Use

    Tags

    tufA-His-CBD

    Expression

    Bacterial

    Mutation

    codon optimized Ecoli, residues 45-313

    Promoter

    T7

    Available since

    April 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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