We narrowed to 43,548 results for: cha;
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Plasmid#53693PurposeLuciferase reporter assay for RECK 3'UTR that has point mutations on miR-21 binding siteDepositorInsertRECK-3'UTR (RECK Human)
UseLuciferaseTagsExpressionMutationnucleotide sequence 1083-1086 is mutated to TTCGPromoterAvailable sinceJuly 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
AAV2_hSyn_Phobos_Citrine
Plasmid#98216PurposeBlue-shifted artificial anion conducting channelrhodopsin (aACR). Activation max 466 nm; off-kinetics 10 ms. Codon optimized for mammalian expression.DepositorInsertSynthetic construct Phobos gene
UseAAVTagsCitrineExpressionMammalianMutationT59S, E83N, E90Q, E101S, V117R, E123S, T159G, G16…Promoterhuman synapsinAvailable sinceAug. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGL3-IRES-Lin28b-P3
Plasmid#64794PurposeLuciferase expression driven by Lin28b promoter P3DepositorInsertLin28b promoter P3 (LIN28B Human)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceJuly 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET21b-LOV-ipaA L514K L531E
Plasmid#40253DepositorInsertLOV-ipaA L514K L531E
UseTags6xHisExpressionBacterialMutationL514K; L531E; Jα helix region hybridized (AsLOV2 …PromoterT7Available sinceSept. 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-LC3-K51A
Plasmid#155265PurposeMammalian expression of rat LC3 fused to EGFPDepositorInsertmicrotubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
UseTagspEGFPExpressionMammalianMutationLysine 51 to AlaninePromoterAvailable sinceMay 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-LC3-R70A
Plasmid#155264PurposeMammalian expression of rat LC3 fused to EGFPDepositorInsertmicrotubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
UseTagspEGFPExpressionMammalianMutationArginine 70 to AlaninePromoterAvailable sinceMay 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP TEM4 R130D
Plasmid#58895PurposeExpresses GFP Tagged full length TEM4 protein with R130D mutation. Please see notes below for information regarding the L722F mutation in TEM4.DepositorInsertTEM4 (ARHGEF17 Human)
UseTagsEGFPExpressionMammalianMutationR130D, L722FPromoterCMVAvailable sinceSept. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
Rat Alpha2 delta-1 mutMIDAS pMT2
Plasmid#58730Purposeexpression of rat alpha2 delta-1 with MIDAS site mutations in mammalian cellsDepositorInsertalpha2 delta-1 (Cacna2d1 Rat)
UseTagsExpressionMammalianMutationMIDAS site mutations (D259VSGS to AVAGA)PromoterSV40Available sinceOct. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFUSEss-CHIg-mG1_Arg322Lys
Plasmid#105849PurposeExpression plasmid coding for mutated heavy chain of mouse IgG1 antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Arg322Lys (EU numbering).DepositorInsertIgG1 heavy chain (Ighg1 Mouse)
UseTagsExpressionMammalianMutationArg322Lys in constant region of IgG1 heavy chainPromoterhEF1-HTLVAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLEX307-MERS-CoV-3CL-C148A
Plasmid#160283PurposeAllows for constituitive mammalian expression of the MERS-CoV 3CL protease with C145A mutation via transfection or lentivirusDepositorInsert3CL Protease
UseLentiviralTagsExpressionMammalianMutationC148APromoterEF1aAvailable sinceOct. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMS04 [myo-3p::bPGC::SL2::mCherry]
Plasmid#168172PurposeExpression of bPGC in BWMs of C. elegansDepositorInsertbPGC
UseTagsExpressionWormMutationPromoterAvailable sinceMay 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP TEM4 DEL 125-135
Plasmid#58896PurposeExpresses GFP tagged full length TEM4 protein with amino acids 125 - 135 deletedDepositorInsertTEM4 (ARHGEF17 Human)
UseTagsEGFPExpressionMammalianMutationDeleted aa's 125-135PromoterCMVAvailable sinceSept. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLEX307-IBV-3CL-C143A
Plasmid#160289PurposeAllows for constituitive mammalian expression of the IBV 3CL protease with C143A mutation via transfection or lentivirusDepositorInsert3CL Protease
UseLentiviralTagsExpressionMammalianMutationC143APromoterEF1aAvailable sinceOct. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pαH-S-HRV3C/D614G
Plasmid#164568PurposeSARS-CoV-2 Spike protein with furin site mutated to HRV3C site and D614G mutation (S-HRV3C-D614G variant)DepositorInsertSpike (S-HRV3C-D614G variant)
UseTags2X Strep-Tag II and 8X His tagExpressionMammalianMutationD614GPromoterAvailable sinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO-Egr3-EYFP-WPRE
Plasmid#186417PurposeOverexpression of Egr3 transcript variant 1 CDS under EF-1α promoterDepositorInsertEarly growth response 3 (Egr3 Mouse)
UseAAVTagsEYFPExpressionMutationPromoterEF-1-alphaAvailable sinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCVL.SSA TLR (CCR5 TALEN Sce Spacer)
Plasmid#46941PurposeCodes for the SSA-TLR with the target site for the CCR5 TALEN with the spacer region corresponding to I-Sce I in a lentiviral backboneDepositorInserts5' iRFP arm
eGFP with TALEN TS
+3 mCherry
3' iRFP arm
UseLentiviralTagsExpressionBacterial and MammalianMutationembedded TALEN TS from 163-218, truncated 25 amin…PromoterSFFVAvailable sinceNov. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
FKBP-DCRD-YFP
Plasmid#87450Purposerapamycin-mediated heterodimerization between PCRD and DCRD from C-terminal of L-type Ca2+ channelsDepositorInsertDCRD (distal C-terminal regulatory domain) (CACNA1F Human)
UseTags6xHis-T7, FKBP, and YFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(hPLK1)-PGKpuro2ABFP-W
Plasmid#163138PurposeLentiviral gRNA plasmid targeting human PLK1 gene, co-expression of BFP tagDepositorInsertPLK1 (PLK1 Human)
UseCRISPR and LentiviralTagsBFPExpressionMammalianMutationPromoterU6Available sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
p-AAV2-hSyn-MerMAID6-Citrine
Plasmid#126521PurposeAnion-conducting Channelrhodopsin with near-complete desensitization in continuous light. Codon-optimized for mammalian expression (human/mouse).DepositorInsertMerMAID6
UseAAVTagsCitrineExpressionMutationPromoterhuman synapsinAvailable sinceNov. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRC/RSV-rat Agt
Plasmid#122104PurposeExpression of angiotensinogenDepositorInsertangiotensinogen (Agt Rat)
UseTagsExpressionMammalianMutationPromoterRSV promoterAvailable sinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pECE-M2-BAIAP2 S366A
Plasmid#31657DepositorInsertBAI1-associated protein 2 S366A (BAIAP2 Human)
UseTagsM2ExpressionMammalianMutationchanged Serine 366 to AlaninePromoterAvailable sinceApril 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
CFP-PCRD-FRB
Plasmid#87449Purposerapamycin-mediated heterodimerization between PCRD and DCRD from C-terminal of L-type Ca2+ channelsDepositorInsertPCRD (proximal C-terminal regulatory domain) (CACNA1F Human)
UseTags6xHis-T7, CFP, and FRBExpressionMammalianMutationPromoterCMVAvailable sinceMarch 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23 T230A 2014 EBOV + Mucin-Like Domain
Plasmid#86017PurposeEbola Virus (Makona) Glycoprotein in CMV driven mammalian expression vectorDepositorInsertMakona Ebola Virus Glycoprotein
UseTagsExpressionMammalianMutationChanged threonine 230 to alaninePromoterCMV IEAvailable sinceJan. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23 D637G 2014 EBOV + Mucin-Like Domain
Plasmid#86018PurposeEbola Virus (Makona) Glycoprotein in CMV driven mammalian expression vectorDepositorInsertMakona Ebola Virus Glycoprotein
UseTagsExpressionMammalianMutationChanged aspartic acid 637 to glycinePromoterCMV IEAvailable sinceJan. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
FUW TetO NET1A-V5 WT
Plasmid#69828PurposeExpresses NET1A-V5 in mammalian cells in a doxycycline inducible mannerDepositorInsertNeuroepithelial cell-transforming gene 1 protein A (NET1 Human)
UseLentiviralTagsv5ExpressionMammalianMutationPromoterminimal CMV promoter with tetracycline operatorAvailable sinceApril 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
RIPK4 gRNA (BRDN0001147121)
Plasmid#75969Purpose3rd generation lentiviral gRNA plasmid targeting human RIPK4DepositorInsertRIPK4 (RIPK4 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
GB1-His-TET1-878-911(pL91)
Plasmid#124390PurposeE.Coli produced TET1 SID (TET1-878-911) purified by FPLC nickel columnDepositorInsertGB1-His-TET1-878-911 wildtype (TET1 Human)
UseTagsGB1-HistidineExpressionBacterialMutationPromoterAvailable sinceMay 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pECE M2-SH3PXD2A 8 serines mutated to alanines
Plasmid#69816PurposeExpresses M2-SH3PXD2A in mammalian cellsDepositorInsertSH3 and PX domain-containing protein 2A (SH3PXD2A Human)
UseTagsFLAGExpressionMammalianMutationPromoterSV40 early promoterAvailable sinceMay 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHR-mCherry-p2A-LIC1 G domain
Plasmid#74602Purposeexpresses LIC1 in mammalian cells; aa 1-389, untagged but cells indicate expression with diffuse mCherryDepositorInsertfull length human dynein light intermediate chain 1 amino acids 1-389 (DYNC1LI1 Human)
UseLentiviralTagsHA tagExpressionMammalianMutationPromoterSFFVAvailable sinceMay 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKdH_P-GAP-ScADH2+P-GAP*-ScALD6+ScACS1*
Plasmid#126739Purposeyeast plasmid overexpressing ADH2,ALD6 and ACS1 from Saccharomyces cerevisiaeDepositorInsertadh2, acs1*, ald6
UseTags6xHis,MycExpressionYeastMutationT3542C and A30S, S629N (please see depositor comm…PromoterGAPAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pECE M2-SH3PXD2A 6 serines mutated to alanines
Plasmid#69815PurposeExpresses M2-SH3PXD2A in mammalian cellsDepositorInsertSH3 and PX domain-containing protein 2A (SH3PXD2A Human)
UseTagsFLAGExpressionMammalianMutationPromoterSV40 early promoterAvailable sinceMay 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.2/V5-DEST NET1A S46A
Plasmid#69818PurposeExpresses NET1A-V5 in mammalian cellsDepositorInsertNeuroepithelial cell-transforming gene 1 protein A (NET1 Human)
UseTagsV5ExpressionMammalianMutationS46APromoterCMVAvailable sinceApril 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23 A82V/T230A 2014 EBOV + Mucin-Like Domain
Plasmid#86019PurposeEbola Virus (Makona) Glycoprotein in CMV driven mammalian expression vectorDepositorInsertMakona Ebola Virus Glycoprotein
UseTagsExpressionMammalianMutationChanged alanine 82 to valine and threonine 230 to…PromoterCMV IEAvailable sinceJan. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
I-GGTT
Plasmid#43138DepositorInsertI-GGTT
UseBacterial cloningTagsExpressionMutationPromoterAvailable sinceJuly 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
II-CTCG
Plasmid#43337DepositorInsertII-CTCG
UseBacterial cloningTagsExpressionMutationPromoterAvailable sinceJuly 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
RIPK4 gRNA (BRDN0001144783)
Plasmid#75970Purpose3rd generation lentiviral gRNA plasmid targeting human RIPK4DepositorInsertRIPK4 (RIPK4 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
RIPK4 gRNA (BRDN0001146610)
Plasmid#75971Purpose3rd generation lentiviral gRNA plasmid targeting human RIPK4DepositorInsertRIPK4 (RIPK4 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-human LIC1 G domain
Plasmid#74598Purposeused for expression in E. coli and purification. N-terminal GST tag and C-terminal Strep tag, aa 1-389DepositorInserthuman dynein light intermediate chain 1 amino acids 1-389 (DYNC1LI1 Human)
UseTagsN-terminal GST and Strep IIExpressionBacterialMutationPromoterT7Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
IV-AAA
Plasmid#43731DepositorInsertIV-AAA
UseBacterial cloningTagsExpressionMutationPromoterAvailable sinceJuly 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
tdEos-Keratin-17
Plasmid#57629PurposeNote- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 505 / 569, Emission: 516 / 581DepositorInsertKeratin (KRT18 Human)
UseTagstdEosExpressionMammalianMutationV282LPromoterCMVAvailable sinceJan. 13, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits