We narrowed to 29,515 results for: REP

1. pAR-B1(1986.1)

   Plasmid

   #78503

   Purpose

   Trans plasmid for production of recombinant AAV-B1 vectors. Plasmid encodes AAV2 Rep and AAV-B1 capsid

   Depositor

   Miguel Sena-Esteves

   Article

   Choudhury et al Mol Ther. 2016 Apr 27. doi: 10.1038/mt.2

   Inserts

   AAV2 Rep
   AAV-B1 capsid
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pαH-S-RRAR

   Plasmid

   #164569

   Purpose

   SARS-CoV-2 Spike protein with Furin site restored (S-RRAR variant)

   Depositor

   Priyamvada Acharya

   Article

   Gobeil et al Cell Rep. 2021 Jan 12;34(2):108630. doi:

   Insert

   Spike (S-RRAR variant)
   

   Use

   Tags

   2X Strep-Tag II, 8X His tag, and HRV3C cleavage s…

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-FLAG-puro-PTPIP51(1-150_175-470)

   Plasmid

   #170537

   Purpose

   Expresses PTPIP51 FFAT-like motif (151-175) deletion mutant (1-150_176-470) in human HeLa cells after transfection

   Depositor

   Byung Il Lee

   Article

   Yeo et al EMBO Rep. 2021 Jun 4;22(6):e51323. doi:

   Insert

   Protein tyrosine phosphatase interacting protein 51 (RMDN3 Human)
   

   Use

   Tags

   FLAG tag

   Expression

   Mammalian

   Mutation

   deleted amino acids 151-175

   Promoter

   CAG promoter

   Available since

   Aug. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

4. pMXs-E55A-ATPIF1

   Plasmid

   #85404

   Purpose

   Retroviral vector expressing human ATPIF1 with E55A mutation, which abrogates the interaction between ATPIF1 and the ATP synthase

   Depositor

   David Sabatini

   Article

   Chen et al Cell Rep. 2014 Apr 10;7(1):27-34. doi: 1

   Insert

   ATPIF1 (ATP5IF1 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   E55A point mutation abrogating the ability of ATP…

   Promoter

   Available since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pelB-MBP-P66-ss

   Plasmid

   #137045

   Purpose

   E. coli expression clone (T7lac promoter) for mature P66 (aa 22-618) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage

   Depositor

   Debra Hansen

   Article

   Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

   Insert

   AAC66949.1 (p66 Borrelia burgdorferi B31)
   

   Use

   Tags

   PelB signal peptide + His10 + maltose binding pro…

   Expression

   Bacterial

   Mutation

   mature P66: lacks the P66 signal peptide

   Promoter

   T7lac

   Available since

   April 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFUSEss-CHIg-mM_M18

   Plasmid

   #91729

   Purpose

   Expression plasmid coding for heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18.

   Depositor

   Joanna Bereta

   Article

   Klaus et al Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1

   Insert

   immunoglobulin heavy constant mu (Ighm Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hEF1-HTLV prom

   Available since

   June 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRSFDuet-1_VNp-LZ_VNp-LZ-hGH

   Plasmid

   #182412

   Purpose

   Bacterial expression of Vesicle Nucleating peptide-Leucine Zipper and Vesicle Nucleating peptide-Leucine zipper-human growth hormone fusion

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

   Inserts

   VNp-LZ-hGH (GH1 Human)
   VNp-LZ
   

   Use

   Tags

   Vesicle Nucleating peptide (VNp)

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-VTKS5

   Plasmid

   #170856

   Purpose

   AAV backbone for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-OFF) of any transgene in neurons under the control of the hSyn1 promoter.

   Depositor

   Jordane Dimidschstein

   Article

   Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

   Type

   Empty backbone

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDNA4TO K164A H165A CoV-2 nsp1 3xFLAG

   Plasmid

   #176060

   Purpose

   Expresses K164A K165A CoV2 nsp1 in mammalian cells. This contains a C-terminal 3xFLAG tag fusion to nsp1.

   Depositor

   Britt Glaunsinger

   Article

   Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

   Insert

   CoV-2 K164A H165A nsp1 (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   K164A and H165A

   Promoter

   CMV

   Available since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pS6-TGFB2-H8

    Plasmid

    #128502

    Purpose

    Expresses human pro-TGFβ2 (from L21 to S414) in mammalian cells. With N-t H8x tag.

    Depositor

    F Xavier Gomis-Ruth

    Article

    Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

    Insert

    TGFB2 (L21-S414) (TGFB2 Human)
    

    Use

    Tags

    His tag (6x)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPN458

    Plasmid

    #137876

    Purpose

    PiggyBac vector for expression of 1x gRNA targeting TCF4 for CRISPRa;mRFP-T2A-BlasticidinR

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

    Insert

    TCF4 (TCF4 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. LV-hHOXA13

    Plasmid

    #135561

    Purpose

    Express hHOXA13 and EGFP in mammalian cells

    Depositor

    Jose Teixeira

    Article

    George et al Cell Rep. 2019 Dec 17;29(12):4069-4085.e

    Insert

    Homeobox A13 (HOXA13 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CBH

    Available since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcmv3-STING-C1471S-FLAG

    Plasmid

    #198188

    Purpose

    Mammalian expression of FLAG-tagged mouse STING1 C1471S

    Depositor

    Yun Zhao

    Article

    Li et al Cell Rep. 2023 Mar 28;42(3):112145. doi:

    Insert

    TMEM173 (Sting1 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    The cysteine in STING at position 147 mutates to …

    Promoter

    CMV

    Available since

    April 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRSFDuet-1_VNp-LZ_VNp-LZ-mNeongreen-hGH

    Plasmid

    #182425

    Purpose

    Bacterial expression of Vesicle Nucleating peptide-Leucine Zipper and Vesicle Nucleating peptide-Leucine Zipper-mNeongreen-human growth hormone fusion proteins

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Inserts

    VNp-LZ-mNeongreen-hGH (GH1 Human)
    VNp-LZ
    

    Use

    Tags

    Vesicle Nucleating peptide (VNp) and Vesicle Nucl…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDNA4TO R124A K125A CoV-2 nsp1 3xFLAG

    Plasmid

    #176059

    Purpose

    Expressing R124A K125A CoV2 nsp1 in mammalian cells. This contains a C-terminal 3xFLAG tag fusion to nsp1.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    CoV-2 nsp1 R124A/K125A (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    R124A and K125A

    Promoter

    CMV

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-VTKD5-GCaMP6f

    Plasmid

    #178728

    Purpose

    AAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-OFF) of GCaMP6f in cortical interneurons under the control of the Dlx enhancer (Dimidschstein et al 2016)

    Depositor

    Jordane Dimidschstein

    Article

    Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

    Insert

    GCaMP6f
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRetroX-TRE3G_FLAG-CRTC1/MAML2_PGK-GpNLuc

    Plasmid

    #154265

    Purpose

    Dox/Tet-inducible expression of the CRTC1-MAML2 fusion oncogene in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Musicant et al Cell Rep. 2021 Feb 23;34(8):108768. doi:

    Insert

    FLAG-CRTC1/MAML2 fusion oncogene
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. p-mCherry-C1-Phobos_CA

    Plasmid

    #98169

    Purpose

    slow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin (aACR). High light sensitivity. Activation with blue light, inactivation max with 580 nm (accelarates closure to ms). Codon optimized for mammalian expression.

    Depositor

    Peter Hegemann

    Article

    Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.

    Insert

    Synthetic construct Phobos_C128A gene
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    T59S, E83N, E90Q, E101S, V117R, E123S, C128A, T15…

    Promoter

    CMV (+enhancer)

    Available since

    July 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSBbi-RP-CoV2/stSpike-puro

    Plasmid

    #161794

    Purpose

    Transposon-based SARS-CoV-2 secretable trimeric Spike stable expression vector for protein production

    Depositor

    Jorge Luis Arias-Arias

    Article

    Arias-Arias et al Biotechnol Rep (Amst). 2023 Mar;37:e0078

    Insert

    SARS-CoV-2 secretable trimeric Spike
    

    Use

    Transposon

    Tags

    6xHis and dTomato

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a/RPBSA

    Available since

    Aug. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLV-tetO-HA-GFI1B

    Plasmid

    #125599

    Purpose

    doxycycline-inducible expression of human GFI1B (HA tagged) in mammalian cells

    Depositor

    Filipe Pereira

    Article

    Gomes et al Cell Rep. 2018 Dec 4;25(10):2821-2835.e7

    Insert

    Growth Factor Independent 1B transcriptional repressor (GFI1B Human)
    

    Use

    Lentiviral

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    Promoter

    TRE-mCMV

    Available since

    June 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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