We narrowed to 8,449 results for: Ott

1. CaMKII-somBiPOLES-mCerulean

   Plasmid

   #154948

   Purpose

   Optogenetic tool for blue-light inhibition and red-light excitation of neurons

   Depositor

   Simon Wiegert

   Article

   Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

   Has Service

   AAV Retrograde, AAV5, and AAV9

   Insert

   somBiPOLES
   

   Use

   AAV

   Tags

   soma-targeting motif from Kv2.1 channel

   Expression

   Mammalian

   Mutation

   Promoter

   CaMKIIa(0.4)

   Available since

   Aug. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. plx307 SHOC2 V5 WT

   Plasmid

   #190568

   Purpose

   SHOC2 variant overexpression

   Depositor

   William Hahn

   Article

   Kwon et al Nature. 2022 Jul 13. pii: 10.1038/s41586

   Insert

   SHOC2 (SHOC2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLCA.66/2272

   Plasmid

   #22733

   Depositor

   Mark Magnuson

   Article

   Chen et al Dis Model Mech. 2011 Feb 14. ():.

   Insert

   Lox66-pU(delta)TK-EM7Neo-Lox2272
   

   Use

   Cre/Lox; Bac recombineering

   Tags

   Expression

   Mutation

   This vector was designed for assembling gene targ…

   Promoter

   Available since

   Dec. 14, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. pmCherry-N1 GBP (GBP-mCherry)

   Plasmid

   #162879

   Purpose

   Expression in mammalian cells of GFP binding protein (GBP) tagged with mCherry

   Depositor

   Frederic Meunier

   Article

   Gormal et al Proc Natl Acad Sci U S A. 2020 Nov 19. p

   Insert

   GFP Binding Protein
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEN35-CDKN2A-Ex2-R

   Plasmid

   #110737

   Purpose

   Encodes Cas9 nickase and gRNA targeting CDKN2A Exon2

   Depositor

   Robert Judson-Torres

   Article

   Zeng et al Cancer Cell. 2018 Jul 9;34(1):56-68.e9.

   Insert

   CDKN2A gRNA (CDKN2A Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAGGS D614 soluble Spike

   Plasmid

   #164652

   Purpose

   Expresses SARS-CoV-2 soluble Spike protein

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

   Insert

   SARS-CoV-2 soluble spike (S SARS-CoV-2)
   

   Use

   Tags

   HIS

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Feb. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

7. HNF4A_P2-2200

   Plasmid

   #31062

   Depositor

   Gerhart Ryffel

   Article

   Thomas et al Hum Mol Genet. 2001 Sep 15. 10(19):2089-

   Insert

   HNF4A P2 promoter (HNF4A Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   -2200 to -1 of P2 HNF4A promoter

   Promoter

   Available since

   Oct. 14, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEN35-CDKN2A-Ex2-L

   Plasmid

   #110736

   Purpose

   Encodes Cas9 nickase and gRNA targeting CDKN2A Exon2

   Depositor

   Robert Judson-Torres

   Article

   Zeng et al Cancer Cell. 2018 Jul 9;34(1):56-68.e9.

   Insert

   CDKN2A gRNA (CDKN2A Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. lentiCRISPR - EGFP sgRNA 4

   Plasmid

   #51763

   Purpose

   Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

   Depositor

   Feng Zhang

   Article

   Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:

   Inserts

   Cas9
   Puromycin resistance
   EGFP sgRNA 4
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EFS and U6

   Available since

   March 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. GFP-ataxin-3 C14A

    Plasmid

    #89977

    Purpose

    Expresses catalytic inactive GFP-tagged ataxin-3

    Depositor

    Nico Dantuma

    Article

    Pfeiffer et al EMBO J. 2017 Mar 8. pii: e201695151. doi

    Insert

    ataxin-3 (ATXN3 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    changed Cysteine 14 to Alanine

    Promoter

    Available since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. lentiCRISPR - EGFP sgRNA 5

    Plasmid

    #51764

    Purpose

    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

    Depositor

    Feng Zhang

    Article

    Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:

    Inserts

    Cas9
    Puromycin resistance
    EGFP sgRNA 5
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EFS and U6

    Available since

    March 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTriEx-RhoA-Q63L_mScarlet-i_SGFP2

    Plasmid

    #85072

    Purpose

    Green/red FRET biosensor for RhoA in the GTP locked constitutively active state

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Insert

    RhoA Biosensor constitutively active (Q63L) (RHOA Human)
    

    Use

    Tags

    6xHis and mScarlet-i and SGFP2

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Q63L

    Promoter

    CMV

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. AAV-hSyn-Ribo-jGCaMP8m

    Plasmid

    #167574

    Purpose

    DEPRECATED: Please see Addgene item #169248 for an improved version of this plasmid. AAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8m.

    Depositor

    Marianne Fyhn

    Article

    Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

    Has Service

    AAV1

    Insert

    Ribo-GCaMP8m
    

    Use

    AAV

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAcGFP1-N1-Dsup-C

    Plasmid

    #90022

    Purpose

    To express GFP-tagged C-terminal region of Dsup in mammalian cells transiently

    Depositor

    Takekazu Kunieda

    Article

    Hashimoto et al Nat Commun. 2016 Sep 20;7:12808. doi: 10

    Insert

    Dsup
    

    Use

    Tags

    AcGFP1

    Expression

    Mammalian

    Mutation

    C-terminal region alone

    Promoter

    CMV

    Available since

    May 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. AT4G23740 J11_pECIA2

    Plasmid

    #114998

    Purpose

    Bait vector AT4G23740 J11_pECIA2 should be used with prey vector AT4G23740 J11_pECIA14.

    Depositor

    Youssef Belkhadir

    Article

    Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

    Insert

    AT4G23740 (AT4G23740 Mustard Weed)
    

    Use

    Tags

    Fc, V5 and hexahistidine tags

    Expression

    Insect

    Mutation

    Promoter

    Metallothionein (Copper-Inducible)

    Available since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-EF1a-DIO-FLPo-Myc

    Plasmid

    #124641

    Purpose

    AAV-mediated Cre-dependent expression of FLPo recombinase under EF1a promoter

    Depositor

    David Dupret

    Article

    Trouche et al Cell. 2019 Mar 7;176(6):1393-1406.e16. d

    Insert

    FLPo
    

    Use

    AAV and Cre/Lox

    Tags

    myc tag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    May 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGAPTrap-eGFP-IRESMygro

    Plasmid

    #82503

    Purpose

    Vector targeting the eGFP gene to the GAPDH locus of human cells. eGFP is expressed from a 2A sequence at the C terminus of GAPDH. Hygromycin B resistance is encoded by the Mygro gene.

    Depositor

    Ed Stanley

    Article

    Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221

    Insert

    eGFP-IRESMygro
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGAPTrap-mCherry-IRESMygro

    Plasmid

    #82505

    Purpose

    Vector targeting the mCherry gene to the GAPDH locus of human cells. mCherry is expressed from a 2A sequence at the C terminus of GAPDH. Hygromycin B resistance is encoded by the Mygro gene.

    Depositor

    Ed Stanley

    Article

    Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221

    Insert

    mCherry-IRESMygro
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. MSCV-BRD9-PGK-Puro-IRES-GFP

    Plasmid

    #75114

    Purpose

    Retroviral expression plasmid encoding wild type human BRD9

    Depositor

    Christopher Vakoc

    Article

    Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

    Insert

    BRD9 (BRD9 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 19, 2016

    Availability

    Academic Institutions and Nonprofits only

20. pTBL210

    Plasmid

    #126451

    Purpose

    Expresses Cas9-XTEN-TdT in mammalian cells.

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Insert

    Cas9-XTEN-TdT (DNTT Streptococcus pyogenes, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    See depositor comments below

    Promoter

    CMV

    Available since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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