We narrowed to 9,203 results for: sacs

1. pCG_batCoV RaTG13 NSP15 C-V5-tag

   Plasmid

   #179984

   Purpose

   Mammalian expression vector for RaTG13 Nsp15, V5-tagged. Sequence codon-optimized.

   Depositor

   Konstantin Sparrer

   Article

   Hayn et al Cell Rep. 2021 May 18;35(7):109126.

   Insert

   RaTG13 Nsp15
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   human codon-optimized

   Promoter

   Available since

   Feb. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. Flag-KLF6-4D (1054)

   Plasmid

   #49490

   Purpose

   expresses human Flag tagged KLF6 with 4D mutation

   Depositor

   Scott Friedman

   Article

   Lang et al Oncogene. 2012 Oct 22. doi: 10.1038/onc.

   Insert

   KFL6-4D (KLF6 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   three serines and one tyrosine mutated to aspart…

   Promoter

   CMV

   Available since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pTO_Snf7_LAP

   Plasmid

   #101851

   Purpose

   Tet-inducible expression of Snf7 from S. cerevisiae with a C-terminal GFP and long linker (LAP tag), compatible with Flp-In recombination for stable integration

   Depositor

   Daniel Gerlich

   Article

   Mierzwa et al Nat Cell Biol. 2017 Jul;19(7):787-798. d

   Insert

   SNF7 (SNF7 Budding Yeast)
   

   Use

   Gateway cloning, flp-frt recombination, tet-induc…

   Tags

   Localization and Affinity Purification (LAP) tag

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. sgALK(B)

   Plasmid

   #85563

   Purpose

   Expresses sgRNA targeting human ALK around codon F1174

   Depositor

   Luca Grumolato

   Article

   Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:

   Insert

   anaplastic lymphoma receptor tyrosine kinase (ALK Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

5. AAV phSyn1(S)-FlpE-bGHpA

   Plasmid

   #50364

   Purpose

   Can be used to generate AAV virus that will express FlpE recombinse gene in neurons from the synapsin promoter

   Depositor

   Hongkui Zeng

   Article

   Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

   Insert

   FlpE recombinase gene
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn1

   Available since

   Jan. 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. mTagBFP2-FKBP-CYB5Atail

   Plasmid

   #108141

   Purpose

   For recruitment of ER

   Depositor

   Gerry Hammond

   Article

   Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

   Insert

   mTagBFP2:FKBP1A(3-108):[GGSA]4GG:CYB5A(100-134) (CYB5A, FKBP1A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   CYB5A(100-134)

   Promoter

   CMV

   Available since

   April 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. shCCL20

   Plasmid

   #65096

   Purpose

   retroviral expression of CCL20 shRNA

   Depositor

   Pau Sancho-Bru

   Article

   Affo et al Gut. 2014 Nov;63(11):1782-92. doi: 10.11

   Insert

   Ccl20 shRNA (Ccl20 Mouse)
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   June 12, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pNIA-CEN-mVenus-FLAG-LANS1-Set2

   Plasmid

   #122003

   Purpose

   mVenus-FLAG-LANS-Set2: mVenus- and FLAG-tagged LANS1-Set2 for light control of Set2 localization in yeast

   Depositor

   Brian Kuhlman

   Article

   Lerner et al Genome Res. 2020 Nov;30(11):1605-1617. d

   Insert

   mVenus-FLAG-LANS1-Set2 (SET2 Budding Yeast)
   

   Use

   Synthetic Biology

   Tags

   mVenus-FLAG

   Expression

   Yeast

   Mutation

   Set2 point mutations K538G, K539S, R549G, K550S, …

   Promoter

   ADH1

   Available since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPICZ:K2P2.1(mTREK-1)Cryst S112C

   Plasmid

   #224780

   Purpose

   Pichia pastoris expression vector for generating mouse TREK-1(21S-322T) + 3C cleavable site + eGFP + 10x His under the AOX1 promoter

   Depositor

   Dan Minor

   Article

   Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

   Insert

   Potassium channel subfamily K member 2 (Kcnk2 Mouse)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   K84R / Q85E / T86K / I88L / A89R / Q90A / A92P / …

   Promoter

   AOX1 promoter

   Available since

   Sept. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDual

    Plasmid

    #207883

    Purpose

    Expresses thermosensitive variants of the homing endonucleases I-OnuI and I-GpeMI, each in a different open reading frame. Both were made thermosensitive by a fusion with the L212P VMA1 intein.

    Depositor

    David Edgell

    Article

    Foo et al Nucleic Acids Res. 2024 Feb 28;52(4):206

    Inserts

    Thermosensitive fusion of I-OnuI and the L212P VMA1 intein.
    Thermosensitive fusion of I-GpeMI and the L212P VMA1 intein.
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Leucine 212 changed to proline, leucine 434 in th…

    Promoter

    Available since

    Aug. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA5-FRT-TO-EGFR*-CUbo(K63R)-His6

    Plasmid

    #212820

    Purpose

    Constitutive or doxycycline-inducible expression of EGFR*-CUbo(K63R)-His6 in mammalian cells

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    EGFR* (EGFR Human, Budding Yeast)
    

    Use

    Tags

    CUbo(K63R)-6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA5-FRT-TO-EGFR*-CUbo(K48R)-GFP

    Plasmid

    #212816

    Purpose

    Constitutive or doxycycline-inducible expression of EGFR*-CUbo(K48R)-GFP in mammalian cells

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    EGFR* (EGFR Human, Budding Yeast)
    

    Use

    Tags

    CUbo(K48R)-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA5-FRT-TO-EGFR*-CUbo(K63R)-GFP

    Plasmid

    #212817

    Purpose

    Constitutive or doxycycline-inducible expression of EGFR*-CUbo(K63R)-GFP in mammalian cells

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    EGFR* (EGFR Human, Budding Yeast)
    

    Use

    Tags

    CUbo(K63R)-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA5-FRT-TO-EGFR*-CUbo(K48R K63R)-GFP

    Plasmid

    #212818

    Purpose

    Constitutive or doxycycline-inducible expression of EGFR*-CUbo(K48R K63R)-GFP in mammalian cells

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    EGFR* (EGFR Human, Budding Yeast)
    

    Use

    Tags

    CUbo(K48R/K63R)-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. Coexpressed Free deadFPPS-NES

    Plasmid

    #182492

    Purpose

    Yeast integrative plasmid for expressing ERG20 (GAL10 promoter) and fusion protein deadFPPS-AcNES1 (GAL7 promoter). deadFPPS is ERG20(K197G-K254A), an inactive mutant of ERG20.

    Depositor

    Claudia Vickers

    Article

    Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.

    Inserts

    FPPS
    deadFPPS-NES
    

    Use

    Synthetic Biology; Metabolic engineering

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL10 and GAL7

    Available since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCG_SARS-CoV1 C-V5-tag ORF7a

    Plasmid

    #179971

    Purpose

    Mammalian expression vector for SARS-CoV-1 ORF7a, V5-tagged. Sequence codon-optimized.

    Depositor

    Konstantin Sparrer

    Article

    Hayn et al Cell Rep. 2021 May 18;35(7):109126.

    Insert

    SARS-CoV-1 ORF7a (ORF7a Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCG_SARS-CoV1 NSP7 C-V5-tag

    Plasmid

    #179963

    Purpose

    Mammalian expression vector for SARS-CoV-1 Nsp7, V5-tagged. Sequence codon-optimized.

    Depositor

    Konstantin Sparrer

    Article

    Hayn et al Cell Rep. 2021 May 18;35(7):109126.

    Insert

    SARS-CoV-1 Nsp7 (ORF1ab Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    human codon-optimized

    Promoter

    Available since

    Oct. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCG_batCoV RaTG13 ORF7a C-V5-tag

    Plasmid

    #179989

    Purpose

    Mammalian expression vector for RaTG13 ORF7a, V5-tagged. Sequence codon-optimized.

    Depositor

    Konstantin Sparrer

    Article

    Hayn et al Cell Rep. 2021 May 18;35(7):109126.

    Insert

    RaTG13 ORF7a
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    human codon-optimized

    Promoter

    Available since

    Feb. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCG_batCoV RaTG13 NSP16 C-V5-tag

    Plasmid

    #179985

    Purpose

    Mammalian expression vector for RaTG13 Nsp16, V5-tagged. Sequence codon-optimized.

    Depositor

    Konstantin Sparrer

    Article

    Hayn et al Cell Rep. 2021 May 18;35(7):109126.

    Insert

    RaTG13 Nsp16
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    human codon-optimized

    Promoter

    Available since

    Feb. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCG_SARS-CoV1 ORF6 C-V5-tag

    Plasmid

    #179970

    Purpose

    Mammalian expression vector for SARS-CoV-1 ORF6, V5-tagged. Sequence codon-optimized.

    Depositor

    Konstantin Sparrer

    Article

    Hayn et al Cell Rep. 2021 May 18;35(7):109126.

    Insert

    SARS-CoV-1 ORF6 (ORF6 Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    human codon-optimized

    Promoter

    Available since

    Feb. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCG_SARS-CoV1 E C-V5-tag

    Plasmid

    #179966

    Purpose

    Mammalian expression vector for SARS-CoV-1 E, V5-tagged. Sequence codon-optimized.

    Depositor

    Konstantin Sparrer

    Article

    Hayn et al Cell Rep. 2021 May 18;35(7):109126.

    Insert

    SARS-CoV-1 E (E Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    human codon-optimized

    Promoter

    Available since

    Feb. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCG_SARS-CoV1 NSP16 C-V5-tag

    Plasmid

    #179965

    Purpose

    Mammalian expression vector for SARS-CoV-1 Nsp16, V5-tagged. Sequence codon-optimized.

    Depositor

    Konstantin Sparrer

    Article

    Hayn et al Cell Rep. 2021 May 18;35(7):109126.

    Insert

    SARS-CoV-1 Nsp16 (ORF1ab Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    human codon-optimized

    Promoter

    Available since

    Feb. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. CPH3568 (YCp LEU2 Rpb1 P1455_E1456InsGGGGGLEVLFQGP(H)10,N1501K; msLink1)

    Plasmid

    #91819

    Purpose

    Yeast expression of S. cerevisiae Rpb1 with an internal PreScission protease site and 10xHis tag for purification of the linker-CTD

    Depositor

    Chris Hill

    Article

    Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

    Insert

    RPO21 (RPO21 Budding Yeast)
    

    Use

    Tags

    10xHis

    Expression

    Yeast

    Mutation

    5xGly linker, PreScission Protease site (LEVLFQGP…

    Promoter

    Endogenous Rpb1 promoter

    Available since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCDFDuet-MKK6-EE p38alpha

    Plasmid

    #82719

    Purpose

    Expresses constitutively active human MKK6 and murine p38 alpha.

    Depositor

    Kevin Janes

    Article

    Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

    Inserts

    MKK6 (MAP2K6 Human)
    p38alpha (Mapk14 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Ser207 to Glu, Thr211 to Glu

    Promoter

    Available since

    Feb. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

25. GFP-α-cateninWT

    Plasmid

    #229709

    Purpose

    Transient expression of GFP-alpha-cateninWT in mammalian cells

    Depositor

    Alpha Yap

    Article

    Noordstra et al Dev Cell. 2023 Sep 25;58(18):1748-1763.e

    Insert

    CTNNA1 (alpha-catenin) Human (CTNNA1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pTR01F/hFGFR2c-V5

    Plasmid

    #236013

    Purpose

    for PiggyBac mediated integration and stable expression of hFGFR2c protein

    Depositor

    Pavel Krejčí

    Article

    Zlinska et al Mol Ther Nucleic Acids. 2024 Nov 28;36(1

    Insert

    human FGFR2c (FGFR2 Human)
    

    Use

    Tags

    V5/His

    Expression

    Mammalian

    Mutation

    Promoter

    truncated CMV promoter

    Available since

    April 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pTR01F/hFGFR2b-V5

    Plasmid

    #236014

    Purpose

    for PiggyBac mediated integration and stable expression of hFGFR2b protein

    Depositor

    Pavel Krejčí

    Article

    Zlinska et al Mol Ther Nucleic Acids. 2024 Nov 28;36(1

    Insert

    human FGFR2b (FGFR2 Human)
    

    Use

    Tags

    V5/His

    Expression

    Mammalian

    Mutation

    Promoter

    truncated CMV promoter

    Available since

    April 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Human IFN-gamma promoter/enhancer

    Plasmid

    #17598

    Depositor

    Howard Young

    Article

    Gonsky et al J Immunol. 2000 Feb 1. 164(3):1399-407.

    Insert

    IFN gamma promoter with 1st intron enhancer (IFNG Human)
    

    Use

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    -3.6 kb to +70 of promoter plus 680 bp of IFN-gam…

    Promoter

    Available since

    March 27, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. sgAPC(A)

    Plasmid

    #85557

    Purpose

    Expresses sgRNA targeting human APC around codon I1417

    Depositor

    Luca Grumolato

    Article

    Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:

    Insert

    APC, WNT signaling pathway regulator (APC Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

30. sgAPC(B)

    Plasmid

    #85558

    Purpose

    Expresses sgRNA targeting human APC around codon I1417

    Depositor

    Luca Grumolato

    Article

    Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:

    Insert

    APC, WNT signaling pathway regulator (APC Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 8, 2017

    Availability

    Academic Institutions and Nonprofits only