We narrowed to 20,115 results for: IRE;

Showing: 8011 - 8040 of 20115 results

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WT Nedd4L

Plasmid

#200086

Purpose

Nedd4L in pQCXIN vector

Depositor

Jason MacGurn

Article

Qualls-Histed et al iScience. 2023 Feb 6;26(3):106150. doi:

Insert

Nedd4L (NEDD4L Human)

Use

Retroviral

Expression

Mammalian

Promoter

CMV

Available Since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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Pr-deGFP-MGapt

Plasmid

#67734

Purpose

OR2-OR1-Pr promoter expressing deGFP-MG aptamer fusion

Depositor

Richard Murray

Article

Siegal-Gaskins et al ACS Synth Biol. 2014 Jun 20;3(6):416-25.

Insert

UTR1-deGFP-MGapt-T500

Use

Synthetic Biology

Expression

Bacterial

Promoter

OR2-OR1-Pr

Available Since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pMBA323

Plasmid

#214751

Purpose

To create delta(glmS,nadE) working strains and maintaining their viability

Depositor

Tae Seok Moon

Article

Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

Insert

BBa_J23105-cas9 + pBAD-λ Red genes + ptet-gRNA (pBR322ori)

Use

CRISPR

Expression

Bacterial

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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DIRAS3

Plasmid

#55686

Purpose

expression of human DIRAS3

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

DIRAS3 (DIRAS3 Human)

Expression

Bacterial

Promoter

lac

Available Since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only
pCS2 GFP-GSK3-MAPK-E3mut

Plasmid

#29691

Depositor

Edward De Robertis

Article

Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

Insert

GFP glycogen synthase kinase 3 recognition site E3 ligase site mutated primed by MAPK

Tags

Flag and GFP

Expression

Mammalian

Mutation

recognition sites only, and E3 ligase site mutated

Available Since

June 9, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+)-Rab10-FLAG

Plasmid

#135559

Purpose

Express mouse Rab10 in mammalian cells, FLAG at N terminal

Depositor

Jingshi Shen

Article

Gulbranson et al Proc Natl Acad Sci U S A. 2017 Sep 26;11

Insert

Rab10 (Rab10 Mouse)

Tags

Flag

Expression

Insect

Promoter

T7 promoter

Available Since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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Tt-NylC_pET28a

Plasmid

#215431

Purpose

Expression construct for Tt-NylC gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

Tt-NylC

Expression

Bacterial

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-3-SH3BP1-CTD

Plasmid

#136649

Purpose

Expression of SH3BP1-CTD-GST fusion protein in E. coli

Depositor

Maria Balda, Karl Matter

Article

Elbediwy et al J Cell Biol. 2012 Aug 20;198(4):677-93.

Insert

SH3BP1-CTD (SH3BP1 Human)

Expression

Bacterial

Mutation

Contains only C-terminal domain

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-3-SH3BP1-NTD

Plasmid

#136648

Purpose

Expression of SH3BP1-NTD-GST fusion protein in E. coli

Depositor

Maria Balda, Karl Matter

Article

Elbediwy et al J Cell Biol. 2012 Aug 20;198(4):677-93.

Insert

SH3BP1-NTD (SH3BP1 Human)

Expression

Bacterial

Mutation

Contains only N-terminal domain

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pUPD2+pCmYLCV911

Plasmid

#170878

Purpose

Phytobrick - pCmYLCV911

Depositor

Markita Landry

Article

Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

Insert

pCmYLCV911

Expression

Plant

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pMAL669

Plasmid

#169148

Purpose

Encodes OptoQ-AMP2

Depositor

Jose Avalos

Article

Lalwani et al ACS Synth Biol. 2021 Aug 20;10(8):2060-2

Insert

VP16-EL222

Tags

SV40 NLS

Expression

Yeast

Available Since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pMAL722

Plasmid

#169149

Purpose

Encodes OptoQ-AMP3

Depositor

Jose Avalos

Article

Lalwani et al ACS Synth Biol. 2021 Aug 20;10(8):2060-2

Insert

VP16-EL222

Tags

SV40 NLS

Expression

Yeast

Mutation

A79Q

Available Since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pMAL716

Plasmid

#169150

Purpose

Encodes OptoQ-AMP4

Depositor

Jose Avalos

Article

Lalwani et al ACS Synth Biol. 2021 Aug 20;10(8):2060-2

Insert

VP16-EL222

Tags

SV40 NLS

Expression

Yeast

Available Since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pHA-ΔN332-Kin1C

Plasmid

#31608

Depositor

Anthony Brown

Article

Uchida et al Mol Biol Cell. 2009 Dec . 20(23):4997-50

Insert

ΔN332 kinesin-1C (Kif5c Mouse)

Tags

HA-tag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 4, 2011

Availability

Academic Institutions and Nonprofits only
GL2 shRNA

Plasmid

#86083

Purpose

A lentiviral construct for building stable cell lines expressing GL2 shRNA via puromycin selection.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

control shRNA

Promoter

U6

Available Since

May 26, 2017

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmGW182

Plasmid

#22419

Depositor

Elisa Izaurralde

Article

Behm-Ansmant et al Genes Dev. 2006 Jul 15. 20(14):1885-98.

Insert

gawky, variant A (gw Fly)

Use

Drosophila expression

Tags

EGFP

Available Since

Dec. 10, 2009

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmGIGYF_AH

Plasmid

#148918

Purpose

Insect Expression of DmGIGYF

Depositor

Elisa Izaurralde

Article

Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

Insert

DmGIGYF (Gyf Fly)

Expression

Insect

Mutation

one silent mutation compared to the sequence give…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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p3003 pGEM E6-AP

Plasmid

#8644

Depositor

Peter Howley

Article

Huibregtse et al Mol Cell Biol 1993 Aug;13(8):4918-27.

Insert

E6-AP (UBE3A Human)

Expression

Bacterial

Available Since

June 16, 2005

Availability

Academic Institutions and Nonprofits only
pEF-1α_ZF9LOV[C2]-VP64 (pLZA126)

Plasmid

#203911

Purpose

Encodes engineered opto-ZF9 with AsLOV2(408-543) inserted in C2 position in mammalian cells

Depositor

Jared Toettcher

Article

Zhu et al Nat Commun. 2023 Jun 2;14(1):3185. doi:

Insert

ZF9LOV[C2]-VP64

Expression

Mammalian

Promoter

EF1α

Available Since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

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pEF-1α_ZF9LOV[C1]-VP64 (pLZA125)

Plasmid

#203910

Purpose

Encodes engineered opto-ZF9 with AsLOV2(408-543) inserted in C1 position in mammalian cells

Depositor

Jared Toettcher

Article

Zhu et al Nat Commun. 2023 Jun 2;14(1):3185. doi:

Insert

ZF9LOV[C1]-VP64

Expression

Mammalian

Promoter

EF1α

Available Since

July 24, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG-FLEX.iGABASnFR.F102Y.Y137L

Plasmid

#112168

Purpose

GABA Sensor

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

Insert

iGABASnFR.F102Y.Y137L

Use

AAV

Mutation

F102Y.Y137L

Promoter

CAG

Available Since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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ORF31-2xStrep pCDNA4

Plasmid

#129744

Purpose

Expresses KSHV ORF31 with Cterm 2xStrep tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

Insert

ORF31 (ORF31 Kaposi's sarcoma-associated herpesvirus (HHV-8))

Tags

2xStrep Tag II

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pLNHA-C1-HsUPF2-siRNAres_I

Plasmid

#146567

Purpose

Mammalian Expression of HsUPF2-siRNAres

Depositor

Elisa Izaurralde

Article

Kashima et al Genes Dev. 2010 Nov 1. 24(21):2440-50.

Insert

HsUPF2-siRNAres (UPF2 Human)

Expression

Mammalian

Available Since

March 10, 2022

Availability

Academic Institutions and Nonprofits only

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RASD2

Plasmid

#55672

Purpose

expression of mouse RASD2

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

RASD2 (Rasd2 Mouse)

Expression

Bacterial

Promoter

lac

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
pQP-2460

Plasmid

#138973

Purpose

Light-induced recruitment of QPAS1-mCherry to the target protein bound to membrane; interaction occurs via intrabody fused to BphP1

Depositor

Vladislav Verkhusha

Article

Redchuk et al Nat Commun. 2020 Jan 30;11(1):605. doi:

Insert

NcoI-mVenus-CAAX-IRES2-BphP1- iB(GFP)- IRES2-NES-mCherry-Q-PAS1-NotI

Available Since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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pET14b_tev_PSGU

Plasmid

#59738

Purpose

Expresses a.a. 417-887 of human ZO-1

Depositor

Alan Fanning

Article

Nomme et al J Biol Chem. 2011 Dec 16;286(50):43352-6

Insert

zonula occludens-1 (TJP1 Human)

Tags

HIS tag and thrombin clvg

Expression

Bacterial

Promoter

T7

Available Since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only
pSuper-Rem2-2

Plasmid

#51595

Purpose

shRNA 2 against rodent Rem2

Depositor

Suzanne Paradis

Article

Moore et al PLoS One. 2013 Aug 26;8(8):e74751. doi:

Insert

Rem2 (Rem2, Rem2 Rat, Mouse)

Use

RNAi

Promoter

U6

Available Since

April 7, 2014

Availability

Academic Institutions and Nonprofits only
Geph CR3m shRNA

Plasmid

#121069

Purpose

Negative control shRNA for plasmid 121068: shRNA targeting the coding region of the gephyrin mRNA

Depositor

Angel de Blas

Article

Yu et al Mol Cell Neurosci. 2007 Dec;36(4):484-50

Insert

GPHN shRNA (Gphn Rat)

Use

RNAi

Available Since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pLentiGuide_GFP_sgEPAS1#1

Plasmid

#183119

Purpose

pLentiGuide modified to express GFP and an sgRNa targeting EPAS1

Depositor

William Kaelin

Article

Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

Insert

sgEPAS1-1

Use

Lentiviral

Promoter

U6

Available Since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pSopB2xHA

Plasmid

#112513

Purpose

PsopB driven sopB-2xHA and sigE

Depositor

Olivia Steele-Mortimer

Article

Knodler et al Cell Microbiol. 2009 Nov;11(11):1652-70.

Insert

sopB-2xHA/sigE operon

Tags

2xHA

Expression

Bacterial

Mutation

SopB C-terminal 2xHA

Promoter

PsopB

Available Since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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