We narrowed to 46,665 results for: cha;

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pLenti-CaMKIIa-hChR2(H134R)-EYFP-WPRE

Plasmid

#20944

Purpose

3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to EYFP driven by CaMKIIa promoter for optogenetic activation

Depositor

Karl Deisseroth

Article

Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

Insert

channelrhodopsin-2

Use

Lentiviral

Tags

EYFP

Expression

Mammalian

Mutation

H134R

Available Since

May 13, 2009

Availability

Academic Institutions and Nonprofits only
L1_lacZgRNA-Ck3

Plasmid

#136136

Purpose

L1 in position 3, to clone gRNA target using BbsI, lacZ blue-white screening

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-MpU6:lacZgRNA

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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LCC-ICCG_pET21b

Plasmid

#215408

Purpose

Expression construct for LCC-ICCG gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

LCC-ICCG

Expression

Bacterial

Mutation

F243I/D238C/S283C/Y127G

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pMXs-Runx1

Plasmid

#131609

Purpose

expression of mouse Runx1 in mammalian cells

Depositor

Kateri Moore, Filipe Pereira

Article

Pereira et al Cell Stem Cell. 2013 Aug 1;13(2):205-18.

Insert

Runx1 (Runx1 Mouse)

Use

Retroviral

Expression

Bacterial

Available Since

Dec. 6, 2019

Availability

Academic Institutions and Nonprofits only

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ChromosomesMembranesandCytoskeleton

Plasmid

#62450

Purpose

MXS_chaining vector with 4 cassettes

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Inserts

CMV::H2B-TagBFPx3-bGHpA
CMV::Lyn-Ceruleanx3-bGHpA
CMV::mCherryx3-linker-betaActin-bGHpA
CMV::Citrinex3-linker-alphaTubulin-bGHpA

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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PB-TRE-dCas9-KRAB-MeCP2

Plasmid

#122267

Purpose

SP-dCas9-KRAB-MeCP2 with doxycycline-inducible expression

Depositor

Andrea Califano

Article

Tan et al bioRxiv 2021.06.28.449297

Insert

dCas9-KRAB-MeCP2

Tags

dCas9-KRAB-MeCP2

Expression

Mammalian

Available Since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

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NylB'-SCY_pET21b

Plasmid

#215417

Purpose

Expression construct for NylB'-SCY gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

NylB'-SCY

Expression

Bacterial

Mutation

R187S/F264C/D370Y

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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L_NLS 41kDa

Plasmid

#201342

Purpose

Nucleocytoplasmic transport probe

Depositor

Pere Roca-Cusachs

Article

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

(NLS)SV40A4-EGFP-2PrA

Expression

Mammalian

Available Since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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pET19b-EcMscL-TEV-mEGFP

Plasmid

#165097

Purpose

Expresses EcMscLGFP as a fusion protein in E. coli BL21or using in vitro expression.

Depositor

Neha Kamat

Article

Jacobs et al Proc Natl Acad Sci U S A. 2019 Feb 13. p

Insert

E. Coli Mechanosensitive Channel of Large Conductance (mscL E. coli)

Use

Synthetic Biology

Tags

monomeric Enhanced Green Fluorescent Protein

Expression

Bacterial

Promoter

T7

Available Since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pPMVAd3

Plasmid

#98298

Purpose

Enhancing the lower-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of consitutive promoters.

Depositor

Claudia Vickers

Article

Peng et al Metab Eng. 2017 Jan;39:209-219. doi: 10.

Insert

PRPL8B-ERG12-TNAT5-PSSB1-ERG8-TIDP1-PRPL3-MVD1-TIDP1-PYEF3-IDI1-TRPL15A

Expression

Yeast

Available Since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pAF0687 Pa01_attP-mCherry donor

Plasmid

#193466

Purpose

Promoterless mcherry donor for Pa01

Depositor

Patrick Hsu

Article

Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

Insert

Pa01_attP

Expression

Mammalian

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pET-16b-10His-TEV-MCES_VACCA_D1_D12

Plasmid

#203671

Purpose

The D1 and D12 subunits of the vaccinia capping enzyme were cloned in tandem into the vector pET-16b (Novagen). An N-terminal His10 tag was added to the D1 subunit and the original factor Xa cleavage

Depositor

Stephen Cusack

Article

Kyrieleis et al Structure. 2014 Mar 4;22(3):452-65. doi:

Insert

MCES_VACCA_D1_D12

Tags

10xHis

Expression

Bacterial

Available Since

Aug. 3, 2023

Availability

Academic Institutions and Nonprofits only

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L1_Cas9-Ck4

Plasmid

#136135

Purpose

L1 in position 4, for AtcoCas9 (Puchta) expression in L2 CRISPR vectors

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-MpEF1a:Cas9_3tNos-35S

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pKP112 (pRS406-GFP-ADH1t)

Plasmid

#106472

Purpose

Vector for creating C-terminal GFP fusion proteins in yeast.

Depositor

Daniel Klionsky

Article

Parzych et al Mol Biol Cell. 2018 May 1;29(9):1089-109

Type

Empty backbone

Tags

GFP

Expression

Bacterial and Yeast

Promoter

None

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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L1_lacZgRNA-Ck2

Plasmid

#136137

Purpose

L1 in position 2, to clone gRNA target using BbsI, lacZ blue-white screening

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-MpU6:lacZgRNA

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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h-alpha-ENaC

Plasmid

#83430

Purpose

full length human alpha ENaC with Flag tag

Depositor

Christie Thomas

Article

Raikwar et al Am J Physiol Renal Physiol. 2008 May;294

Insert

human alpha ENaC (SCNN1A Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pAM57

Plasmid

#227654

Purpose

pETDuet-1: Rpn8(1-179)-PreScission-Strep, H6-PreScission-Rpn11(2-239, V80A)

Depositor

Andreas Martin

Article

Worden et al Nat Struct Mol Biol. 2014 Mar;21(3):220-

Inserts

Rpn11 (RPN11 Budding Yeast)
Rpn8 (RPN8 Budding Yeast)

Tags

H6-PreScission and PreScission-Strep

Expression

Bacterial

Mutation

1-179 and amino acids 2-239, V80A

Promoter

T7

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pAM71

Plasmid

#227621

Purpose

pETDuet-1 H6-precission-Rpn11(24-160-GGSGSG-192-221) , Rpn8(1-179)

Depositor

Andreas Martin

Article

Worden et al Mol Cell. 2017 Sep 7;67(5):799-811.e8. d

Inserts

Rpn11 (RPN11 Budding Yeast)
rpn8 (RPN8 Budding Yeast)

Tags

His-Prescission

Expression

Bacterial

Mutation

1-179 and amino acids 24-160-GGSGSG-192-221

Promoter

T7

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pPD0029_pIVTRup(BsmBI)_SpCas9

Plasmid

#242535

Purpose

IVT

Depositor

Christoph Bock, Paul Datlinger

Article

Datlinger et al Nature. 2025 Oct;646(8086):963-972. doi:

Insert

SpCas9

Use

CRISPR

Available Since

Nov. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pZac2.1 gfaABC1D-BioID2-BioID2-HA

Plasmid

#176740

Purpose

Expresses the promiscuous small biotin ligase, BioID2, in astrocytes

Depositor

Baljit Khakh

Article

Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

Insert

BioID2-BioID2-HA

Use

AAV

Tags

HA

Expression

Bacterial

Promoter

gfaABC1D

Available Since

April 11, 2023

Availability

Academic Institutions and Nonprofits only

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