We narrowed to 5,913 results for: pcDNA 3.1

1. WN10150

   Plasmid

   #80443

   Purpose

   Expresses dead (908A) AsCpf1

   Depositor

   Ervin Welker

   Article

   Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

   Insert

   nuclease dead (908A) AsCpf1
   

   Use

   Tags

   NLS-3xHA

   Expression

   Mammalian

   Mutation

   nuclease dead (D908A)

   Promoter

   Available since

   Aug. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. CMV-mito-LAR-GECO1.2

   Plasmid

   #61245

   Purpose

   Expresses LAR-GECO1.2 in the mitochondria in mammalian cells

   Depositor

   Robert Campbell

   Article

   Wu et al Biochem J. 2014 Nov 15;464(1):13-22. doi

   Insert

   LAR-GECO1.2
   

   Use

   Tags

   a duplex of the mitochondrial targeting signal of…

   Expression

   Mammalian

   Mutation

   Substitutions relative to R-GECO1.2: N45I/A47R/E1…

   Promoter

   CMV

   Available since

   March 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. Mito-mGreenLantern

   Plasmid

   #164461

   Purpose

   Localization of mGreenLantern fluorescent protein to mitochondria

   Depositor

   Gregory Petsko

   Article

   Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

   Insert

   mGreenLantern
   

   Use

   Tags

   Human COX8a first 29 amino acids

   Expression

   Mammalian

   Mutation

   Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…

   Promoter

   CMV

   Available since

   Jan. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. HA-MDFI

   Plasmid

   #207836

   Purpose

   Tagged MDFI for western blotting and imaging

   Depositor

   Charles Cox

   Article

   Zhou et al Science. 2023 Aug 18;381(6659):799-804.

   Insert

   MDFI (MDFI Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. RANLS

   Plasmid

   #50843

   Purpose

   Expresses one ddFP in mammalian cells, the second plasmid for a green to red colour switch-based fluorescent caspase-3 biosensor, used together with plasmid GANES-DEVD-BNLS.

   Depositor

   Robert Campbell

   Article

   Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

   Insert

   ddRFP A
   

   Use

   Tags

   triplicated NLS sequence DPKKKRKV

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. CAGGS-AsCpf1-2A-GFP-U6-sgRNA-cloning vector

   Plasmid

   #159281

   Purpose

   A multicistronic vector with both CAGGS promoter-driven AsCpf1 and U6 promoter-driven single guide RNA (sgRNA)

   Depositor

   Rudolf Jaenisch

   Article

   Ma et al Cell Rep Med. 2020 Sep 22;1(6):100097. d

   Insert

   AsCpf1-HA-2A-GFP
   

   Use

   Multicistronic vector with both caggs promoter-dr…

   Tags

   3X HA

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. GANLS

   Plasmid

   #50852

   Purpose

   Expresses one ddFP in mammalian cells, the second plasmid for a red to green colour switch-based fluorescent caspase-3 biosensor, used together with plasmid NESRA-DEVD-BNLS.

   Depositor

   Robert Campbell

   Article

   Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

   Insert

   ddGFP A
   

   Use

   Tags

   triplicated NLS sequence DPKKKRKV

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

8. β₂AR-mCFP

   Plasmid

   #38260

   Depositor

   Robert Lefkowitz

   Article

   Violin et al J Biol Chem. 2006 Jul 21;281(29):20577-8

   Insert

   β₂AR (ADRB2 Human)
   

   Use

   Tags

   Flag, mCFP, and signal peptide sequence (MKTIIALS…

   Expression

   Mammalian

   Mutation

   diglycine linker (replacing terminator codon)

   Promoter

   CMV

   Available since

   Aug. 23, 2012

   Availability

   Academic Institutions and Nonprofits only

9. pSL3718 (pQCascade_Pse)

   Plasmid

   #200899

   Purpose

   Expresses human codon-optimized Pseudoalteromonas Cas7, Cas8, Cas6, and TniQ from a CMV promoter, in a polycistronic vector format separated by T2A sequences.

   Depositor

   Samuel H. Sternberg

   Article

   Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

   Insert

   PseCas7, PseCas8, PseCas6, PseTniQ
   

   Use

   Tags

   NLS (TniQ) and NLS, T2A (Cas7, Cas8, Cas6)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. VN-alpha Synuclein

    Plasmid

    #89470

    Purpose

    expresses human alpha Synuclein fused to the N-terminal part of Venus

    Depositor

    Bradley Hyman, Tiago Outeiro

    Article

    Outeiro et al PLoS One. 2008 Apr 2;3(4):e1867. doi: 10

    Insert

    SNCA (SNCA Human)
    

    Use

    Tags

    N-terminal part of Venus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. MC4R

    Plasmid

    #233630

    Purpose

    encodes the human melanocortin 4 receptor, without any tags

    Depositor

    Christian Gruber

    Article

    Muratspahic et al ACS Pharmacol Transl Sci. 2023 Sep 26;6(

    Insert

    MC4R (MC4R Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/T7

    Available since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. MLF2-GFP-3xFLAG

    Plasmid

    #230986

    Purpose

    Expresses GFP tagged MLF2 in mammalian cells

    Depositor

    Christian Schlieker

    Article

    Prophet et al Nat Cell Biol. 2022 Nov;24(11):1630-1641

    Insert

    MLF2 (MLF2 Human)
    

    Use

    Tags

    3xFLAG and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. CCM2_wt_eGFP

    Plasmid

    #208876

    Purpose

    Express eGFP-CCM2 in mammalian cells.

    Depositor

    Guillaume Jacquemet

    Article

    Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003

    Insert

    CCM2 (CCM2 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    WT sequence

    Promoter

    Available since

    Dec. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. HiBiT-FZD7

    Plasmid

    #195845

    Purpose

    Expresses human FZD7 with an N-terminal HiBiT

    Depositor

    Gunnar Schulte

    Article

    Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

    Insert

    FZD7 (FZD7 Human)
    

    Use

    Tags

    HiBiT

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. cyt-GafD-mTurboID-V5

    Plasmid

    #184640

    Purpose

    Expresses V5-tagged GlycoID construct: GafD_short-miniTurboID in mammalian cytosol for O-GlcNAc interactome tagging

    Depositor

    Charlie Fehl

    Article

    Liu et al ACS Chem Biol. 2022 Jul 12. doi: 10.1021

    Insert

    cyt-GlycoID-V5
    

    Use

    Tags

    Nuclear export sequence and V5 tag

    Expression

    Mammalian

    Mutation

    GafD -> expressed aa22-178 ; BirA* -> expre…

    Promoter

    CMV

    Available since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. Syn-ATP

    Plasmid

    #51819

    Purpose

    Optical reporter of presynaptic ATP

    Depositor

    Timothy Ryan

    Article

    Rangaraju et al Cell. 2014 Feb 13;156(4):825-35. doi: 10

    Insert

    Chimeric Synaptophysin-mCherry-Luciferase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Within WT luciferase gene, changed Threonine 214 …

    Promoter

    CMV

    Available since

    March 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-Target-ACEmax

    Plasmid

    #139105

    Purpose

    Mammalian Target-ACEmax expression vector (pSI-625)

    Depositor

    Nozomu Yachie

    Article

    Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

    Insert

    Bi_partite_NLS-TadA*-XTEN_linker-TadA-XTEN_linker-SpCas9(D10A)-SH3_linker-PmCDA1-UGI-SV40NLS
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    June 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. alpha catenin conformation sensor

    Plasmid

    #71709

    Purpose

    This alpha catenin biosensor undergoes a change in FRET upon mechanical perturbation of the alpha catenin conformation.

    Depositor

    Deborah Leckband

    Article

    Kim et al Curr Biol. 2015 Jan 19;25(2):218-24. doi

    Insert

    alpha-catenin FRET Sensor
    

    Use

    Tags

    ECFP and YPET

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. nuc-GafD-mTurboID-HA

    Plasmid

    #184641

    Purpose

    Expresses 3xHA-tagged GlycoID construct: GafD_short-miniTurboID in mammalian nucleus for O-GlcNAc interactome tagging

    Depositor

    Charlie Fehl

    Article

    Liu et al ACS Chem Biol. 2022 Jul 12. doi: 10.1021

    Insert

    nuc-GlycoID-HA
    

    Use

    Tags

    3xHA tag and Nuclear localization sequence

    Expression

    Mammalian

    Mutation

    GafD -> expressed 22-178 ; BirA* -> express…

    Promoter

    CMV

    Available since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. ER-mGreenLantern

    Plasmid

    #164463

    Purpose

    Localization and retention of mGreenLantern fluorescent protein in the ER

    Depositor

    Gregory Petsko

    Article

    Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    mGreenLantern
    

    Use

    Tags

    Calreticulin and KDEL

    Expression

    Mammalian

    Mutation

    Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…

    Promoter

    CMV

    Available since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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