163,606 results

1. pLEX_306

   Plasmid

   #41391

   Purpose

   Constitutive lentiviral expression, SV40-puro; PGK-gateway-V5 tag

   Depositor

   David Root

   Type

   Empty backbone

   Use

   Lentiviral; Gateway destination vector

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available Since

   Nov. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRL278

   Plasmid

   #70256

   Purpose

   sacB plasmid with polylinker; kanamycin/neomycin resistance: used as a conditionally lethal gene in Anabaena sp. strain PCC 7120 to select for double recombinants and to entrap insertion seq.

   Depositor

   Peter Wolk

   Article

   Cai et al J Bacteriol. 1990 Jun;172(6):3138-45.

   Type

   Empty backbone

   Use

   Conditionally lethal

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. EGFP-Rab7A

   Plasmid

   #28047

   Depositor

   Qing Zhong

   Article

   Sun et al Proc Natl Acad Sci U S A. 2010 Nov 9. 10

   Insert

   Rab7A (RAB7A Human)
   

   Use

   Tags

   2xHA and EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 27, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. PB-tetO-NB

   Plasmid

   #197092

   Purpose

   PiggyBac plasmid with tet-inducible expression of transcription factors for sensory neuron differentiation

   Depositor

   Brian Wainger

   Article

   Held et al Cell Rep. 2023 Aug 30;42(9):113046. doi:

   Insert

   NGN2-T2A-BRN3A (NEUROG2, POU4F1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available Since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPPED

   Plasmid

   #162468

   Purpose

   Destination Expression vector for dicot plant prime editing

   Depositor

   Adam Bogdanove

   Article

   Wang et al Front Genome Ed. 2021 Mar 4;3:617553. do

   Inserts

   Cas9H840A
   attR gateway destination cassette
   

   Use

   Tags

   MLV reverse transcriptase

   Expression

   Plant

   Mutation

   Change histidine 840 to Alanine based sequence o…

   Promoter

   2x35S

   Available Since

   Dec. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-dCas13-M3nls

   Plasmid

   #155366

   Purpose

   Targeted m6A RNA methylation in mammalian cells

   Depositor

   David Liu

   Article

   Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

   Insert

   dCas13-M3nls (METTL3 Cas13b is from Prevotella sp. P5-125; METTL3 is from H. sapiens)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   PspCas13b Δ984-1090 H133A

   Promoter

   Available Since

   July 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMycVec2

   Plasmid

   #32361

   Depositor

   Michael Niederweis

   Article

   Kaps et al Gene. 2001 Oct 31;278(1-2):115-24.

   Insert

   pJAZ38 ori
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Nov. 21, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLKO5.sgRNA.EFS.tRFP

   Plasmid

   #57823

   Purpose

   3rd generation Lentiviral Vector for Sp sgRNA delivery without SpCas9, tagRFP, EFS Promoter driven

   Depositor

   Benjamin Ebert

   Article

   Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

   Inserts

   sgRNA
   EFS
   tagRFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAVS1-Pur-CAG-EGFP

   Plasmid

   #80945

   Purpose

   human AAVS1 site targeting donor plasmid for knocking-in EGFP expression cassette driven by CAG promoter

   Depositor

   Su-Chun Zhang

   Article

   Chen et al Cell Stem Cell. 2016 Jun 2;18(6):817-26.

   Insert

   EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG promoter

   Available Since

   Nov. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. IST1-GFP

    Plasmid

    #186298

    Purpose

    Expresses human IST1 C-terminally fused to GFP

    Depositor

    Geert van den Bogaart

    Article

    Stempels et al J Cell Biol. 2023 Jul 3;222(7):e20220513

    Insert

    IST1 homolog (IST1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    GFP

    Promoter

    CMV

    Available Since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCW57-MCS1-P2A-MCS2 (GFP)

    Plasmid

    #80924

    Purpose

    All-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.

    Depositor

    Adam Karpf

    Article

    Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

    Type

    Empty backbone

    Use

    Lentiviral; Doxycycline inducible; p2a self cleav…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available Since

    Aug. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. CoV2-M-IRES-E

    Plasmid

    #177938

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Membrane and Envelope proteins (original Wuhan Hu1 sequence). Used for generating RNA packaging virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Membrane and Envelope proteins (original Wuhan Hu1 sequence) (E, M )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUltra-Plus-H2B-mTagBFP2

    Plasmid

    #161790

    Purpose

    3rd generation lentiviral bicistronic vector for mammalian expression of H2B-mTagBFP2 and one gene of interest

    Depositor

    Jorge Luis Arias-Arias

    Insert

    H2B-mTagBFP2
    

    Use

    Lentiviral; Bicistronic

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    I174A in the mTagBFP2 protein according to PLoS O…

    Promoter

    UbC and UbC promoter

    Available Since

    Nov. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. BCL2L1_pLX307

    Plasmid

    #98323

    Purpose

    Lentiviral expression of BCL2L1

    Depositor

    William Hahn, Sefi Rosenbluh

    Article

    Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

    Insert

    BCL2L1 (BCL2L1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    E1Fa

    Available Since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDH-EF1-Large BiT - SNAP-tag - Beta-actin

    Plasmid

    #171019

    Purpose

    Lentiviral expression of a Large BiT-SNAP-tag-Beta-actin fusion in mammalian cells

    Depositor

    Angus Johnston

    Article

    Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

    Insert

    LargeBit-SNAPtag- Beta-actin
    

    Use

    Lentiviral and Luciferase

    Tags

    Beta actin and LargeBiT

    Expression

    Mammalian

    Mutation

    Promoter

    EF1

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1 TEV (full-length)

    Plasmid

    #64276

    Purpose

    Expresses tobacco etch virus protease (TEV)

    Depositor

    Xiaokun Shu

    Article

    To et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

    Insert

    Tobacco etch virus (TEV) protease
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCBC-MT1T2

    Plasmid

    #50593

    Purpose

    CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites

    Depositor

    Qi-Jun Chen

    Article

    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

    Insert

    T1, gRNA scaffold, OsU3t plus, TaU3p, T2
    

    Use

    CRISPR; Pcr template

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. AP1muA-Halo-ALFA-polyA-G418 HR

    Plasmid

    #229681

    Purpose

    Homology repair plasmid for endogenous tagging of AP1muA at the C-terminus with HaloTag and an ALFA tag. Contains a G418 resistance cassette for selection of edited cells.

    Depositor

    Francesca Bottanelli

    Article

    Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/

    Insert

    genomic sequence AP1M1 locus (AP1M1 Human)
    

    Use

    CRISPR

    Tags

    Halo, ALFA

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCX-HO1-2A-EGFP

    Plasmid

    #74672

    Purpose

    Mammalian expression of HO1 and EGFP

    Depositor

    Roberto Giovannoni

    Article

    Cinti et al PLoS One. 2015 Oct 29;10(10):e0141933. d

    Insert

    HO1 (HMOX1 Human)
    

    Use

    Tags

    2A-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    pCAGGS

    Available Since

    Feb. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1(Hygro) TagBFP-hNucleolin

    Plasmid

    #182592

    Purpose

    expresses bfp-hNucleolin in mammalian cells

    Depositor

    Agnel Sfeir

    Article

    Laprade et al Mol Cell. 2020 Jul 2;79(1):115-126.e6. d

    Insert

    hNucleolin (NCL Human)
    

    Use

    Tags

    BFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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21. pBS-hsFlt3L

    Plasmid

    #25074

    Depositor

    Maria Castro

    Article

    Curtin et al J Immunol. 2006 Mar 15. 176(6):3566-77.

    Insert

    human soluble FMS-like tyrosine kinase 3 ligand (FLT3LG Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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22. pRN112

    Plasmid

    #84464

    Purpose

    pJB38-NWMN29-30+ SarA_P1-mAmetrine-Term

    Depositor

    Reindert Nijland

    Article

    de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

    Insert

    SarA_P1-mAmetrine from pRN12
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. SMA-Cherry

    Plasmid

    #110209

    Purpose

    Mapping of mesenchymal progenitor cells.

    Depositor

    David Rowe

    Article

    Grcevic et al Stem Cells. 2012 Feb;30(2):187-96. doi:

    Insert

    mCherry
    

    Use

    Tags

    None

    Expression

    Mammalian

    Mutation

    Promoter

    Smooth muscle alpha-actin and first intron

    Available Since

    June 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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24. Creb1

    Plasmid

    #154942

    Purpose

    Expresses Creb1 in mammalian cells

    Depositor

    Georgios Stathopoulos

    Insert

    Creb1 (Creb1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. GUT BuGZ FL

    Plasmid

    #84024

    Purpose

    Expression of GFP tagged BuGZ in human cells

    Depositor

    Patrick Paddison

    Article

    Toledo et al Dev Cell. 2014 Feb 10;28(3):282-94. doi:

    Insert

    BuGZ (ZNF207 Human)
    

    Use

    Lentiviral

    Tags

    TurboGFP

    Expression

    Mammalian

    Mutation

    Q394R

    Promoter

    UCOE / SFFV

    Available Since

    Oct. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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26. hE-cadherin-pcDNA3

    Plasmid

    #45769

    Depositor

    Barry Gumbiner

    Article

    Gottardi et al J Cell Biol. 2001 May 28;153(5):1049-60.

    Insert

    hE-cadherin (CDH1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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27. pX458-Ef1a-dCas9-VPR-H2B-GFP (CRISPRa)

    Plasmid

    #175571

    Purpose

    Inactivated Cas9 (dCas9) fusion to VPR for the purpose of targeted activation

    Depositor

    Joseph Brzezinski

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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28. pRSF-G1(7FTrp)RS

    Plasmid

    #177310

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of 7-Fluoro-L-Tryptophan (7FTrp) into proteins in E. coli in response to the amber (TAG) codon

    Depositor

    Thomas Huber

    Article

    Qianzhu et al ACS Sens. 2022 Jan 10. doi: 10.1021/acss

    Insert

    tRNA synthetase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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29. pDisplay-mgSrtA

    Plasmid

    #125792

    Purpose

    Transfected into cell of interest for membrane expression. The encoded mgSrtA could enable labeling of the interacting cells which is useful for recording cell-cell interactions.

    Depositor

    P. Chen

    Article

    Ge et al J Am Chem Soc. 2019 Feb 6;141(5):1833-18

    Insert

    mgSrtA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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30. gRNA NIA TLS1

    Plasmid

    #196980

    Purpose

    Expresses two gRNAs targeting Arabidopsis NIA1 gene for knockout. Each gRNA is fused to TLS1 mobility signal that confers graft mobility.

    Depositor

    Friedrich Kragler

    Article

    Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    AtNIA1 gRNAs fused to TLS1 tags
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    pU6-26, pU6-29

    Available Since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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