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Showing: 1041 - 1080 of 15188 results

  1. pCV019-catP

    Plasmid
    #232825
    Purpose
    Random transposon mutagenesis vector for Bacteroidales species - chloramphenicol
    Depositor
    Athanasios Typas
    Insert
    catP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    232825-plasmid-map-sequence-id-462956

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  2. pCV020-tetQ

    Plasmid
    #232826
    Purpose
    Random transposon mutagenesis vector for Bacteroidales species - tetracycline
    Depositor
    Athanasios Typas
    Insert
    tetQ
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    232826-plasmid-map-sequence-id-462958

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  3. pCW-LIC

    Plasmid
    #26098
    Purpose
    SGC Empty backbone for bacterial expression
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Use
    Tags
    Three consecutive Taq promoters, 2 active
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 20, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
    26098-plasmid-map-sequence-id-251071

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  4. pET28GST-LIC

    Plasmid
    #26101
    Purpose
    SGC Empty backbone for bacterial expression
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Use
    Tags
    6XHis, GST, and Thrombin CLeavage Site
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 20, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
    26101-plasmid-map-sequence-id-142370

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  5. pZLrhaB2plus

    Plasmid
    #211787
    Purpose
    pSCrhaB2plus with modifications of resistance cassette to CmR
    Depositor
    Silvia Cardona
    Insert
    CmR
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    211787-plasmid-map-sequence-id-426773

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  6. pSARGENT1

    Plasmid
    #216315
    Purpose
    Reporter cassette for SARGENT with piggyBac ITRs and 10x Capture Sequence
    Depositor
    Barak Cohen
    Insert
    tagBFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    216315-plasmid-map-sequence-id-430346

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  8. pGoldenGreenGate-M (pGGG-M)

    Plasmid
    #165422
    Purpose
    A Golden Gate (MoClo) compatible Level 2 acceptor binary vector based on pGreenII (Hellens et al., 2000).
    Depositor
    John Innes Centre - Wheat Transformation and Genome Editing, Wendy Harwood
    Article
    Smedley et al Curr Protoc. 2021 Mar;1(3):e65. doi: 10.
    Type
    Empty backbone
    Use
    Synthetic Biology; Level 2 golden gate (moclo) du…
    Tags
    Expression
    Mutation
    Promoter
    Contain GG cassette and MCS with LacZ for blue/wh…
    Available since
    April 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    165422-plasmid-map-sequence-id-331272

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  9. pL1P4 ZmUbiP:GRF-GIF:NosT

    Plasmid
    #198048
    Purpose
    MoClo Golden Gate Level 1 Position 4. Overexpression cassette of Growth-Regulating Factor 4 (GRF4) plus GRF-Interacting Factor 1 (GRF4-GIF1). Improves in vitro wheat regeneration and transformation.
    Depositor
    Cristobal Uauy, John Innes Centre - Wheat Transformation and Genome Editing
    Insert
    ZmUbiP::TaGRF4-GIF1::NosT
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Zea mays (maize) ubiquitin promoter
    Available since
    April 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    198048-plasmid-map-sequence-id-389382

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  10. Level 1 P2 OsUbiP:Cas9:NosT

    Plasmid
    #165424
    Purpose
    GoldenGate (MoClo) Level 1 Position 2 OsUbiP: Cas9: NosT cassette
    Depositor
    John Innes Centre - Wheat Transformation and Genome Editing, Wendy Harwood
    Article
    Smedley et al Curr Protoc. 2021 Mar;1(3):e65. doi: 10.
    Insert
    Os Ubiquitin promoter :: Cas9 :: NosTerminator
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Os Ubiquitin promoter :: Cas9 :: NosTerminator
    Available since
    April 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    165424-plasmid-map-sequence-id-331265

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  11. Level 1 P4 TaU6 guide acceptor

    Plasmid
    #165600
    Purpose
    GoldenGate (MoClo) Level 1 Position 4 TaU6 guide acceptor plasmid
    Depositor
    John Innes Centre - Wheat Transformation and Genome Editing, Wendy Harwood
    Article
    Smedley et al Curr Protoc. 2021 Mar;1(3):e65. doi: 10.
    Insert
    TaU6 promoter::LacZ::sgRNA scaffold
    Use
    CRISPR and Synthetic Biology; Sgrna acceptor with…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    165600-plasmid-map-sequence-id-331273

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  12. Level 1 P3 TaU6 guide acceptor

    Plasmid
    #165599
    Purpose
    GoldenGate (MoClo) Level 1 Position 3 TaU6 guide acceptor plasmid
    Depositor
    John Innes Centre - Wheat Transformation and Genome Editing, Wendy Harwood
    Article
    Smedley et al Curr Protoc. 2021 Mar;1(3):e65. doi: 10.
    Insert
    TaU6 promoter::LacZ::sgRNA scaffold
    Use
    CRISPR and Synthetic Biology; Sgrna acceptor with…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    165599-plasmid-map-sequence-id-331270

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  13. Level 1 P1 OsActinP:Hygint:NosT

    Plasmid
    #165423
    Purpose
    GoldenGate (MoClo) Level 1 Position 1 (Reverse) hygromycin selection cassette
    Depositor
    John Innes Centre - Wheat Transformation and Genome Editing, Wendy Harwood
    Article
    Smedley et al Curr Protoc. 2021 Mar;1(3):e65. doi: 10.
    Insert
    Os Actin promoter :: Hygromycin resistance gene (hpt) containing CAT1 intron :: NosTerminator
    Use
    Synthetic Biology; L1 p1(r) golden gate (moclo) h…
    Tags
    Expression
    Mutation
    Promoter
    Os Actin promoter :: Hygromycin resistance gene (…
    Available since
    April 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    165423-plasmid-map-sequence-id-331267

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  14. pL1P5 ZmUbiP:GRF-GIF:NosT

    Plasmid
    #198046
    Purpose
    MoClo Golden Gate Level 1 Position 5. Overexpression cassette of Growth-Regulating Factor 4 (GRF4) plus GRF-Interacting Factor 1 (GRF4-GIF1). Improves in vitro wheat regeneration and transformation.
    Depositor
    Cristobal Uauy, John Innes Centre - Wheat Transformation and Genome Editing
    Insert
    ZmUbiP::TaGRF4-GIF1::NosT
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Zea mays (maize) ubiquitin promoter
    Available since
    April 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    198046-plasmid-map-sequence-id-383687

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  15. pGGG Wheat GUS marker

    Plasmid
    #165418
    Purpose
    Wheat transformation binary vector pGGG confers hygromycin resistance in wheat and contains the GUS (2x introns) reporter gene driven by OsUbi promoter
    Depositor
    John Innes Centre - Wheat Transformation and Genome Editing, Wendy Harwood
    Article
    Hayta et al Curr Protoc. 2021 Mar;1(3):e58. doi: 10.
    Insert
    Os Ubiquitin Promoter::Gus (2x introns)::NosT
    Use
    Wheat transformation binary vector
    Tags
    Expression
    Mutation
    Promoter
    OS UbiP::GUS (2xint)::NosT
    Available since
    April 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    165418-plasmid-map-sequence-id-331269

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  16. pL1P3 ZmUbiP:GRF-GIF:NosT

    Plasmid
    #198047
    Purpose
    MoClo Golden Gate Level 1 Position 3. Overexpression cassette of Growth-Regulating Factor 4 (GRF4) plus GRF-Interacting Factor 1 (GRF4-GIF1). Improves in vitro wheat regeneration and transformation.
    Depositor
    Cristobal Uauy, John Innes Centre - Wheat Transformation and Genome Editing
    Insert
    ZmUbiP::TaGRF4-GIF1::NosT
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Zea mays (maize) ubiquitin promoter
    Available since
    March 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    198047-plasmid-map-sequence-id-389251

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  17. pL1P6 ZmUbiP:GRF-GIF:NosT

    Plasmid
    #198049
    Purpose
    MoClo Golden Gate Level 1 Position 6. Overexpression cassette of Growth-Regulating Factor 4 (GRF4) plus GRF-Interacting Factor 1 (GRF4-GIF1). Improves in vitro wheat regeneration and transformation.
    Depositor
    Cristobal Uauy, John Innes Centre - Wheat Transformation and Genome Editing
    Insert
    ZmUbiP::TaGRF4-GIF1::NosT
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Zea mays (maize) ubiquitin promoter
    Available since
    April 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    198049-plasmid-map-sequence-id-391867

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  18. Hg

    Plasmid
    #107573
    Purpose
    4 kb HindII rat genomic fragment containing the entire VGF gene
    Depositor
    Andrea Levi
    Insert
    genomic fragment containing the entire VGF gene (Vgf Rat)
    Use
    Cloning vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    107573-plasmid-map-sequence-id-213994

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  19. FLAG-LoxP-GFP-LoxP-SNAP-TERT

    Plasmid
    #71391
    Purpose
    Homologues recombination donor plasmid for inserting a FLAG-SNAP-tag at the N-terminus of the TERT locus in human cells. Includes GFP marker flanked by LoxP sites for selection.
    Depositor
    Thomas Cech
    Article
    Xi et al Genome Biol. 2015 Nov 10;16(1):231. doi:
    Insert
    TERT (TERT Human)
    Use
    Hr donor
    Tags
    FLAG-SNAP
    Expression
    Mutation
    Promoter
    Endogenous TERT promotor
    Available since
    Dec. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    71391-plasmid-map-sequence-id-128452

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  20. Inducible Caspex expression

    Plasmid
    #97421
    Purpose
    For stable clone generation of cells expressing the dCas9-APEX2 fusion protein for Genomic locus proteomics
    Depositor
    Steven Carr, Samuel Myers
    Article
    Myers et al Nat Methods. 2018 May 7. pii: 10.1038/s4
    Inserts
    Caspex
    T2A-Gfp
    Use
    CRISPR
    Tags
    3xFLAG and V5
    Expression
    Mammalian
    Mutation
    dCas9: D10A and H840A, APEX2: K14D, W41F, E112K, …
    Promoter
    TRE3G
    Available since
    Oct. 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    97421-plasmid-map-sequence-id-466895

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  21. FLAG-LoxP-PURO-LoxP-SNAP-TERT

    Plasmid
    #71390
    Purpose
    Homologues recombination donor plasmid for inserting a FLAG-SNAP-tag at the N-terminus of the TERT locus in human cells. Includes PURO resistance flanked by LoxP sites for selection.
    Depositor
    Thomas Cech
    Article
    Xi et al Genome Biol. 2015 Nov 10;16(1):231. doi:
    Insert
    TERT (TERT Human)
    Use
    Hr donor
    Tags
    FLAG-SNAP
    Expression
    Mutation
    Promoter
    Endogenous TERT promotor
    Available since
    Dec. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    71390-plasmid-map-sequence-id-128451

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  22. pRDB_053

    Plasmid
    #216083
    Purpose
    for transgenic flies; gypsy insulator sequences; white+ selectable marker; attB site for genome integration by phiC31, destination vector
    Depositor
    John Doench
    Article
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Has Service
    Cloning Grade DNA
    Insert
    gypsy insulator sequences; white+ selectable marker; attB site for genome integration by phiC31
    Use
    Destination
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    216083-plasmid-map-sequence-id-431419

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  23. U6_sgRNA_CAG_hSt1Cas9_LMD9

    Plasmid
    #110626
    Purpose
    A single vector mammalian expression system containing a CAG promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD-9) and its U6-driven sgRNA.
    Depositor
    Yannick Doyon
    Article
    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:
    Inserts
    St1Cas9 LMD-9
    sgRNA for St1Cas9
    Use
    CRISPR and Synthetic Biology
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and hU6
    Available since
    May 25, 2018
    Availability
    Academic Institutions and Nonprofits only
    110626-plasmid-map-sequence-id-208002

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  24. HR-TERT-SV40-GFP

    Plasmid
    #71397
    Purpose
    Homologues recombination donor plasmid fo the TERT promotor region introducing a SV-40 driven GFP marker in the TERT promotor. Includes 1kb of TERT coding region in right homologues arm.
    Depositor
    Thomas Cech
    Article
    Xi et al Genome Biol. 2015 Nov 10;16(1):231. doi:
    Insert
    TERT (TERT Human)
    Use
    Tags
    SV-40 GFP insertion in TERT promotor
    Expression
    Mutation
    Promoter
    Endogenous TERT promotor
    Available since
    Dec. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    71397-plasmid-map-sequence-id-128453

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  33. U6_sgRNA_CAG_hSt1Cas9_CNRZ1066

    Plasmid
    #136651
    Purpose
    A single vector mammalian expression system containing a CAG promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD9:CNRZ1066 chimera) and its U6-driven sgRNA.
    Depositor
    Yannick Doyon
    Article
    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:
    Inserts
    St1Cas9 LMD-9:CNRZ_1066 Chimera
    sgRNA for St1Cas9
    Use
    CRISPR and Synthetic Biology
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and hU6
    Available since
    Feb. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    136651-plasmid-map-sequence-id-273320

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Showing: 1041 - 1080 of 15188 results