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Showing: 1301 - 1350 of 14587 results

  9. pBiFC-PBZ-4A-VC

    Plasmid
    #110649
    Purpose
    expresses a PAR-binding (PBZ) domain fused to venus C-terminus, contains point mutations that abolish PAR binding
    Depositor
    Chris Lord
    Article
    Krastev et al Nat Commun. 2018 May 22;9(1):2016. doi:
    Insert
    PBZ domain from APLF, amino acids 371-451 (APLF Human)
    Tags
    HA and VC
    Expression
    Mammalian
    Mutation
    C379A, C385A, C421A and C427A
    Promoter
    CMV
    Available Since
    Aug. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    110649-plasmid-map-sequence-id-217742

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  10. pNOC_dfnCas12a-KRAB_crRNA NC

    Plasmid
    #176262
    Purpose
    pNOC episomal plasmid harboring the dead version of humanized fnCas12a gene sequence tagged to the KRAB domain and Nlux and spacer sequence is replaced by the type IIS restriction site for endonucleas
    Depositor
    John van der Oost
    Article
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Insert
    humanized fnCas12a
    Use
    CRISPR, Luciferase, and Synthetic Biology ; Expre…
    Tags
    Kruppel associated box domain and luciferase
    Mutation
    D917A and E1006A mutations to inactivate the endo…
    Available Since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    176262-plasmid-map-sequence-id-346962

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  11. pNOC_dCas9-KRAB_sgRNA Td2

    Plasmid
    #176264
    Purpose
    pNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged to the KRAB domain and Nlux and sgRNA targeting the fluorescent gene tdtomato with spacer 2
    Depositor
    John van der Oost
    Article
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Insert
    dead spCas9
    Use
    CRISPR, Luciferase, and Synthetic Biology ; Expre…
    Tags
    Kruppel associated box domain and luciferase
    Mutation
    H840A and D10A mutations on spCas9 to inactivate …
    Available Since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    176264-plasmid-map-sequence-id-347050

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  12. pBiFC-PBZ-4A-VN

    Plasmid
    #110647
    Purpose
    expresses a PAR-binding (PBZ) domain fused to venus N-terminus, contains point mutations that abolish PAR binding
    Depositor
    Chris Lord
    Article
    Krastev et al Nat Commun. 2018 May 22;9(1):2016. doi:
    Insert
    PBZ domain from APLF, amino acids 371-451 (APLF Human)
    Tags
    DDK and VN
    Expression
    Mammalian
    Mutation
    C379A, C385A, C421A and C427A
    Promoter
    CMV
    Available Since
    Aug. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    110647-plasmid-map-sequence-id-217748

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  13. pNOC_dfnCas12a-KRAB_crRNA Td2

    Plasmid
    #176261
    Purpose
    pNOC episomal plasmid harboring the dead version of humanized fnCas12a gene sequence tagged to the KRAB domain and Nlux and crRNA targeting the fluorescent gene tdtomato with spacer 2
    Depositor
    John van der Oost
    Article
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Insert
    humanized fnCas12a
    Use
    CRISPR, Luciferase, and Synthetic Biology ; Expre…
    Tags
    Kruppel associated box domain and luciferase
    Mutation
    D917A and E1006A mutations to inactivate the endo…
    Available Since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    176261-plasmid-map-sequence-id-346586

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  18. pRSET-RCaMP1h

    Plasmid
    #42874
    Purpose
    a single-wavelength red-shifted genetically encoded calcium indicator constructed from calmodulin and cp-mRuby
    Depositor
    Loren Looger
    Article
    Akerboom et al Front Mol Neurosci. 2013;6:2. doi: 10.33
    Insert
    Red Fluorescent Calcium binding Protein
    Tags
    EK (Enterokinase) Recognition Site, His Tag (6x),…
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Feb. 22, 2013
    Availability
    Academic Institutions and Nonprofits only
    42874-plasmid-map-sequence-id-58020

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  22. pUC18T-mini-Tn7T-Gm-CC

    Plasmid
    #246943
    Purpose
    Plasmid strain containing pUC18T-mini-Tn7T-Gm-CC01 plasmid, which will insert a nonsense spacer version of the synthetic CRISPR-Cas system from P. aeruginosa PA14 into the genome at the att-Tn7 site.
    Depositor
    Tiffany Taylor
    Article
    Elliott et al Philos Trans R Soc Lond B Biol Sci. 2025
    Insert
    Type IF CRISPR-Cas system from Pseudomonas aeruginosa PA14
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    Native promoters from PA14
    Available Since
    Feb. 5, 2026
    Availability
    Academic Institutions and Nonprofits only
    246943-plasmid-map-sequence-id-499177

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  23. pLCKO-CMV-Blasticidin-P2A-3xHA-TMEM106B_Monkey

    Plasmid
    #238132
    Purpose
    This plasmid encodes the full-length African Green Monkey (Chlorocebus sabaeus) TMEM106B protein fused with a 3xHA tag, along with an N-terminal blasticidin selection marker.
    Depositor
    Dirk Daelemans
    Article
    Baggen et al Cell. 2023 Aug 3;186(16):3427-3442.e22.
    Insert
    TMEM106B
    Use
    Lentiviral
    Tags
    Blasticidin-P2A-3xHA
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    238132-plasmid-map-sequence-id-476568

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  27. pMT646 human L1 ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro

    Plasmid
    #213026
    Purpose
    Expresses full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2
    Depositor
    Martin S. Taylor
    Article
    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:
    Insert
    Human LINE-1 (ORFeus-Hs codon optimized sequence) with ORF2-3xFlag
    Tags
    3xFlag (ORF2p)
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    May 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    213026-plasmid-map-sequence-id-422069

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  28. pRT006.3 L1 dual-luciferase AI retrotransposition reporter

    Plasmid
    #213031
    Purpose
    Bi-directional luciferase antisense intron (firefly fluc AI) human LINE-1 retrotransposition reporter (ORFeus-Hs sequence) for sleeping beauty integration
    Depositor
    Donna Romero, Martin S. Taylor
    Article
    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:
    Insert
    Tet-On Human LINE-1 (ORFeus-Hs) Firefly + Renilla Luciferase Antisense Intron dual retrotransposition marker
    Use
    Luciferase
    Tags
    Firefly luciferase antisense intron (3' UTR)
    Expression
    Mammalian
    Promoter
    pTRE bidirectional tet-on
    Available Since
    July 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    213031-plasmid-map-sequence-id-422025

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  29. pLD564 human L1 ORF2p-3xFlag (L1RP, CMV promoter) in pCEP4 Puro

    Plasmid
    #213030
    Purpose
    Expresses full length human LINE-1 in human cells (native L1RP sequence), with a C-terminal 3xFlag tag on ORF2
    Depositor
    Martin S. Taylor
    Article
    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:
    Insert
    Human LINE-1 (L1RP Sequence) with 5' UTR and ORF2-3xFlag
    Tags
    3xFlag (ORF2p)
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    213030-plasmid-map-sequence-id-422062

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  30. pMT870 human L1 RT- (ORF2p D702Y) ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro (derivative of pMT646)

    Plasmid
    #213027
    Purpose
    Expresses reverse transcriptase dead (RT- ORF2p D702Y) full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2
    Depositor
    Martin S. Taylor
    Article
    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:
    Insert
    Human LINE-1 (ORFeus-Hs codon optimized sequence, RT- D702Y) with ORF2-3xFlag
    Tags
    3xFlag (ORF2p)
    Expression
    Mammalian
    Mutation
    D702Y (RT- reverse transcriptase catalytic dead)
    Promoter
    CMV
    Available Since
    Nov. 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    213027-plasmid-map-sequence-id-452358

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  31. pMT1093 human L1 EN- (ORF2p E43S, D145N) ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro (derivative of pMT646)

    Plasmid
    #213028
    Purpose
    Expresses endonuclease dead (EN- ORF2p E43S, D145N) full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2
    Depositor
    Martin S. Taylor
    Article
    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:
    Insert
    Human LINE-1 (ORFeus-Hs codon optimized sequence, EN- E43S D145N) with ORF2-3xFlag
    Tags
    3xFlag (ORF2p)
    Expression
    Mammalian
    Mutation
    E43S D145N double mutant EN- endonuclease catalyt…
    Promoter
    CMV
    Available Since
    May 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    213028-plasmid-map-sequence-id-422046

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  32. pET28b(+)-HP0377

    Plasmid
    #39334
    Depositor
    SeWon Suh
    Insert
    oxidoreductase
    Tags
    His-tag
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Sept. 17, 2012
    Availability
    Academic Institutions and Nonprofits only

  35. FLX1.8MitoKate2

    Plasmid
    #28132
    Depositor
    Geoffrey Owens
    Insert
    MitoKate2
    Use
    Lentiviral
    Tags
    COX VIII signal sequence
    Expression
    Mammalian
    Available Since
    Oct. 14, 2011
    Availability
    Academic Institutions and Nonprofits only
    28132-plasmid-map-sequence-id-13328

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  36. SbMBP

    Plasmid
    #117861
    Purpose
    protein expression
    Depositor
    Geoffrey Chang
    Insert
    SbMBP
    Expression
    Yeast
    Promoter
    AOX
    Available Since
    Dec. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    117861-plasmid-map-sequence-id-231212

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  37. pET28b(+)-HP0377 C92A

    Plasmid
    #39335
    Depositor
    SeWon Suh
    Insert
    oxidoreductase
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    changed Cysteine 92 to Alanine
    Promoter
    T7
    Available Since
    Sept. 17, 2012
    Availability
    Academic Institutions and Nonprofits only

  38. pQCXIP-turboRFP

    Plasmid
    #73016
    Purpose
    Retroviral expression of turboRFP
    Depositor
    Michael Grusch
    Insert
    TurboRFP
    Use
    Retroviral
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    73016-plasmid-map-sequence-id-264354

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  39. pQCXIN-turboRFP

    Plasmid
    #138275
    Purpose
    Retroviral expression of turboRFP
    Depositor
    Michael Grusch
    Insert
    TurboRFP
    Use
    Retroviral
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    138275-plasmid-map-sequence-id-274573

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  40. HP0421_(7-383)_pET21a

    Plasmid
    #29790
    Depositor
    SeWon Suh
    Insert
    hp0421 (HP0421 H. pylori)
    Tags
    His
    Expression
    Bacterial
    Mutation
    deleted amino acids 1-6 and 384-389
    Available Since
    March 27, 2012
    Availability
    Academic Institutions and Nonprofits only

  41. pYTK001_GFP

    Plasmid
    #220932
    Purpose
    Expresses GFP in bacterial cells
    Depositor
    Ryan Jackson
    Insert
    superfolder GFP
    Expression
    Bacterial
    Available Since
    March 13, 2026
    Availability
    Academic Institutions and Nonprofits only
    220932-plasmid-map-sequence-id-436284

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  42. pYTK001_BFP

    Plasmid
    #220933
    Purpose
    Expresses BFP in bacterial cells
    Depositor
    Ryan Jackson
    Insert
    mTagBFP2
    Expression
    Bacterial
    Available Since
    March 13, 2026
    Availability
    Academic Institutions and Nonprofits only
    220933-plasmid-map-sequence-id-436285

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  43. pSB1A3_OFP

    Plasmid
    #220934
    Purpose
    Expresses OFP in bacterial cells
    Depositor
    Ryan Jackson
    Insert
    OFP BBa_K156009
    Expression
    Bacterial
    Available Since
    March 13, 2026
    Availability
    Academic Institutions and Nonprofits only
    220934-plasmid-map-sequence-id-436809

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  44. pAIP4_3K1H

    Plasmid
    #234157
    Purpose
    Heterologous protein expression of O25709_HELPY in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    3K1H
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234157-plasmid-map-sequence-id-464123

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  45. pUC-L3L2

    Plasmid
    #114019
    Purpose
    Create Entry clones for Gateway LR reactions through simple restriction enzyme based cloning
    Depositor
    Christopher Grefen
    Insert
    ccdB gene, Chloramphenicol resistance gene
    Use
    Gateway
    Available Since
    Jan. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    114019-plasmid-map-sequence-id-222772

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  46. pUC-L1L4

    Plasmid
    #114018
    Purpose
    Create Entry clones for Gateway LR reactions through simple restriction enzyme based cloning
    Depositor
    Christopher Grefen
    Insert
    ccdB gene, Chloramphenicol resistance gene
    Use
    Gateway
    Available Since
    Jan. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    114018-plasmid-map-sequence-id-222765

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  47. pUC-L1L2

    Plasmid
    #114017
    Purpose
    Create Entry clones for Gateway LR reactions through simple restriction enzyme based cloning
    Depositor
    Christopher Grefen
    Insert
    ccdB gene, Chloramphenicol resistance gene
    Use
    Gateway
    Available Since
    Jan. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    114017-plasmid-map-sequence-id-222768

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  49. pBD026

    Plasmid
    #201309
    Purpose
    AvrSr27-2 as Golden Gate compatible MoClo CDS part
    Depositor
    Benjamin Schwessinger
    Insert
    AvrSr27-2
    Expression
    Plant
    Mutation
    removal of BsaI and BpiI sites
    Available Since
    Oct. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    201309-plasmid-map-sequence-id-410982

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  50. pBD025

    Plasmid
    #201308
    Purpose
    AvrSr27-1 as Golden Gate compatible MoClo CDS part
    Depositor
    Benjamin Schwessinger
    Insert
    AvrSr27-1
    Expression
    Plant
    Mutation
    removal of BsaI and BpiI sites
    Available Since
    Oct. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    201308-plasmid-map-sequence-id-409510

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Showing: 1301 - 1350 of 14587 results