We narrowed to 15,964 results for: GRN

1. pUC119-gRNA

   Plasmid

   #52255

   Purpose

   Can use a PCR template to assemble new desired guide RNA. Contains an Arabidopsis U6 promoter to drive guide RNA (targeting AtPDS3 gene target site 1) expression with a TTTTTT as terminator.

   Depositor

   Jen Sheen

   Article

   Li et al Nat Biotechnol. 2013 Aug;31(8):688-91. d

   Insert

   guide RNA targeting AtPDS3 (PDS3 Mustard Weed)
   

   Use

   CRISPR; Plant expression

   Promoter

   Arabidopsis U6 polymerase III promoter

   Available Since

   March 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV hPGRN

   Plasmid

   #213682

   Purpose

   Expresses human progranulin (PGRN) with an N-terminal twin-Strep-V5 tag

   Depositor

   Thomas Kukar

   Article

   Root et al Cell Rep. 2024 Nov 19;43(12):114985. doi

   Insert

   Human Progranulin (hPGRN) (GRN Human)
   

   Use

   AAV

   Tags

   Twin-Strep tag and V5 tag (after signal peptide)

   Promoter

   cytomegalovirus enhancer/chicken β-actin promoter

   Available Since

   Dec. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pU6-gRNA

   Plasmid

   #53062

   Purpose

   Transcription of guide RNA (gRNA) in wheat cells

   Depositor

   Caixia Gao

   Article

   Shan et al Nat Biotechnol. 2013 Aug;31(8):686-8. do

   Insert

   wheat U6 promoter - gRNA
   

   Use

   CRISPR; Plant expression

   Promoter

   wheat U6

   Available Since

   Aug. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGGDestTol2LC-3sgRNA

   Plasmid

   #64241

   Purpose

   destination vector for three U6x:sgRNA cassettes

   Depositor

   Wenbiao Chen

   Article

   Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   May 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGGDestTol2LC-1sgRNA

   Plasmid

   #64239

   Purpose

   destination vector for one U6x:sgRNA cassette

   Depositor

   Wenbiao Chen

   Article

   Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   May 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. AY27_pU6.gRNA.CLYBL

   Plasmid

   #199238

   Purpose

   Expression construct encoding a S. pyogenes guide RNA targeting the human CLYBL safe harbor locus

   Depositor

   Manuel Goncalves

   Article

   Wang et al Nucleic Acids Res. 2023 Mar 17:gkad165.

   Insert

   S. pyogenes gRNA spacer
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Promoter

   RNA polymerase III promoter for human U6 snRNA

   Available Since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX459_gRNA-ROSA26_hspCas9

   Plasmid

   #193310

   Purpose

   Cas9 from S. pyogenes and U6 ROSA26 sgRNA (V2.0)

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Methods Mol Biol. 2023;2676:169-180. doi

   Insert

   Cas9 from S. pyogenes and U6 ROSA26 sgRNA (V2.0)
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   not applicable

   Available Since

   Dec. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcU6_3 sgRNA

   Plasmid

   #92359

   Purpose

   Chicken-specific U6 sgRNA expression mini-vector, harbouring chick U6_3 pol III promoter.

   Depositor

   Tatjana Sauka-Spengler

   Article

   Williams et al Development. 2018 Feb 23;145(4). pii: de

   Insert

   chick U6.3 promoter and gRNA cloning cassette
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   chick U6.3

   Available Since

   Aug. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. VE-cad KI gRNA1

   Plasmid

   #92310

   Purpose

   CRISPR-GFP-gRNA for cutting VEcad

   Depositor

   Sean Palecek

   Article

   Bao et al Bioeng Transl Med. 2017 Jun;2(2):191-201

   Insert

   VEcad KI gRNA1 (CDH5 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   July 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. IL1RN gRNA1

    Plasmid

    #113130

    Purpose

    Expresses gRNA targeting the IL1RN promoter (SpyCas9 scaffold)

    Depositor

    Dominik Niopek

    Article

    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

    Insert

    IL1RN guideRNA 1 (SpCas9 scaffold), co-expressed GFP (transfection marker)
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Expression

    Mammalian

    Promoter

    U6 for gRNA expression, RSV for GFP expression

    Available Since

    March 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. IL1RN gRNA3

    Plasmid

    #113132

    Purpose

    Expresses gRNA targeting the IL1RN promoter (SpyCas9 scaffold)

    Depositor

    Dominik Niopek

    Article

    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

    Insert

    IL1RN guideRNA 3 (SpCas9 scaffold), co-expressed GFP (transfection marker)
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Nov. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. IL1RN gRNA4

    Plasmid

    #113133

    Purpose

    Expresses gRNA targeting the IL1RN promoter (SpyCas9 scaffold)

    Depositor

    Dominik Niopek

    Article

    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

    Insert

    IL1RN guideRNA 4 (SpCas9 scaffold), co-expressed GFP (transfection marker)
    

    Use

    AAV, Synthetic Biology, and TALEN

    Expression

    Mammalian

    Available Since

    Nov. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. IL1RN gRNA2

    Plasmid

    #113131

    Purpose

    Expresses gRNA targeting the IL1RN promoter (SpyCas9 scaffold)

    Depositor

    Dominik Niopek

    Article

    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

    Insert

    IL1RN guideRNA 1 (SpCas9 scaffold), co-expressed GFP (transfection marker)
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Nov. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. U6-AsCas12f-sgRNA-v2

    Plasmid

    #204639

    Purpose

    Expression of trancated AsCas12f sgRNA in mammalian cells

    Depositor

    Weixin Tang

    Article

    Wu et al Nat Chem Biol. 2023 Jul 3. doi: 10.1038/

    Insert

    trancated AsCas12f sgRNA (sgRNA-v2)
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. VE-cad KI gRNA2

    Plasmid

    #92311

    Purpose

    CRISPR-GFP-gRNA for cutting VEcad

    Depositor

    Sean Palecek

    Article

    Bao et al Bioeng Transl Med. 2017 Jun;2(2):191-201

    Insert

    VEcad KI gRNA2 (CDH5 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSMART-sgRNA (Sp)

    Plasmid

    #80427

    Purpose

    U6 promoter driven sgRNA expression plasmid. Annealed oligonucleotides encoding crRNA sequence are ligated into BbsI site.

    Depositor

    Sara Howden, Melissa Little

    Article

    Howden et al Stem Cell Reports. 2016 Sep 13;7(3):508-

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pU3-gRNA

    Plasmid

    #53063

    Purpose

    Transcription of guide RNA (gRNA) in rice cells

    Depositor

    Caixia Gao

    Article

    Shan et al Nat Biotechnol. 2013 Aug;31(8):686-8. do

    Insert

    OsU3 promoter + gRNA scaffold
    

    Use

    CRISPR; Plant expression

    Promoter

    rice U3

    Available Since

    Aug. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLH-stsgRNA3.1

    Plasmid

    #64118

    Purpose

    Vector for expression of St1 sgRNA3.1 in mammalian cells

    Depositor

    Thoru Pederson

    Article

    Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

    Insert

    St1 sgRNA3.1
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTAKN2_J23119-sgRNA

    Plasmid

    #108552

    Purpose

    Bacterial sgRNA expression vector

    Depositor

    Akihiko Kondo

    Article

    Banno et al Nat Microbiol. 2018 Feb 5. pii: 10.1038/

    Insert

    2x Streptococcus pyogenes sgRNA
    

    Expression

    Bacterial

    Promoter

    J23119

    Available Since

    May 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pYLsgRNA-OsU6c

    Plasmid

    #66197

    Purpose

    cloning of sgRNAs for expression in plants

    Depositor

    Yao-Guang Liu

    Article

    Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

    Type

    Empty backbone

    Use

    Cloning vector

    Promoter

    OsU6c

    Available Since

    Aug. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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