We narrowed to 1,535 results for: MK

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pNL1 Ecarin

Plasmid

#106289

Purpose

Expresses Ecarin in Ecoli off a T7 promoter in the NEB control cloning vector

Depositor

James Collins

Article

Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

Insert

Ecarin

Expression

Bacterial

Promoter

T7

Available Since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P30

Plasmid

#120041

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with VSV-G

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

VSV-G

Use

Pcr template

Tags

VSV-G

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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B. thetaiotaomicron mRNA toehold switch sensor

Plasmid

#110707

Purpose

Toehold switch sensor to detect a species specific mRNA with GFP output

Depositor

James Collins

Article

Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

Insert

B. thetaiotaomicron species specific toehold switch sensor

Use

Synthetic Biology

Promoter

T7

Available Since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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Rae1-pDONR221

Plasmid

#124240

Purpose

Gateway entry clone of Drosophila Rae1 cDNA.

Depositor

Peter Vilmos

Article

Kristo et al Biochim Biophys Acta Mol Cell Res. 2017

Insert

Drosophila Rae1 (Rae1 Fly)

Expression

Insect

Available Since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

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pNP1 AND gate sfGFP

Plasmid

#107361

Purpose

Expresses sfGFP (a GFP) in Ecoli off a T7 promoter when pNP1 AND input A and pNP1 AND input B are co-expressed

Depositor

James Collins

Article

Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

Insert

AND gate + sfGFP

Expression

Bacterial

Promoter

T7

Available Since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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PU.1-S148A LZRS

Plasmid

#44633

Depositor

Ellen Rothenberg

Article

Dionne et al Dev Biol. 2005 Apr 15;280(2):448-66.

Insert

PU.1 S148A (Spi1 Mouse)

Use

Retroviral

Tags

EGFP and IRES

Expression

Mammalian

Mutation

148 mutation (S to A)

Available Since

April 22, 2013

Availability

Academic Institutions and Nonprofits only
pMSCV-TASL

Plasmid

#221265

Purpose

Expression of TASL in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

TASL (Tasl Mouse)

Use

Retroviral

Tags

3xFLAG

Available Since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pRetroX-TRE3G-YopE

Plasmid

#221328

Purpose

Dox-inducible expression of YopE in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

YopE

Use

Retroviral

Tags

FLAG

Promoter

TRE3G

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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pRetroX-TRE3G-Tir

Plasmid

#221326

Purpose

Dox-inducible expression of Tir in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

Tir

Use

Retroviral

Tags

HN-FLAG

Promoter

TRE3GV

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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pRetroX-TRE3G-Gag

Plasmid

#221325

Purpose

Dox-inducible expression of Gag in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

Gag

Use

Retroviral

Tags

FLAG

Promoter

TRE3GV

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-FKBP-empty

Plasmid

#221332

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

6xHis-3xFKBP empty scaffold

Expression

Bacterial

Promoter

T7

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-FKBP-ZC3H12B

Plasmid

#221300

Purpose

Expression of ZC3H12B (pLxIS) in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

ZC3H12B (pLxIS)

Use

Retroviral

Tags

FLAG-4xFKBP

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pBiFC-VN155(I152L)-SRCR5

Plasmid

#207993

Purpose

BiFC assay

Depositor

Young Tang

Article

Huang et al Virol J. 2020 Jul 30;17(1):116. doi: 10.

Insert

SRCR5 (CD163 pig)

Expression

Mammalian

Available Since

Dec. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSYn-DIO-ScramblemiR-IRES-mCitrine

Plasmid

#186418

Purpose

Expression of scrambled shRNA used as a control for knock down experiments

Depositor

Mary Kay Lobo

Article

Chandra et al J Neurosci. 2015 May 20;35(20):7927-37.

Insert

ScramblemiR

Use

AAV

Tags

mCitrine

Promoter

human Synapsin1

Available Since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-Egr3-miR-IRES-mCitrine

Plasmid

#186419

Purpose

Expression of shRNA for the knock down of mouse Egr3

Depositor

Mary Kay Lobo

Article

Chandra et al J Neurosci. 2015 May 20;35(20):7927-37.

Insert

miREgr3-5D

Use

AAV

Tags

mCitrine

Promoter

human Synapsin1

Available Since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

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EGFP-RanGAP*

Plasmid

#175237

Purpose

Bacterial expression and purification, low affinity SUMOylation substrate, point mutation F562A reduces affinity for E2, increasing the Km

Depositor

Michael Rosen

Article

Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

Insert

RANGAP1 (RANGAP1 Human)

Expression

Bacterial

Mutation

Amino acid 562 F to A

Promoter

tac

Available Since

Oct. 22, 2021

Availability

Academic Institutions and Nonprofits only

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mCherry-FKBP-E2

Plasmid

#175243

Purpose

Bacterial expression and purification, E2 enzyme (UBC9) for SUMOylation, can be recruited to FRB with rapamycin, CyPet acts as a FRET donor to Ypet

Depositor

Michael Rosen

Article

Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

Insert

UBE2I (UBE2I Human)

Expression

Bacterial

Promoter

T7

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pQP-2376

Plasmid

#138974

Purpose

Light-induced recruitment of NSImb-QPAS1-mCherry to the target protein bound to membrane; interaction occurs via intrabody fused to BphP1

Depositor

Vladislav Verkhusha

Article

Redchuk et al Nat Commun. 2020 Jan 30;11(1):605. doi:

Insert

NcoI-mVenus-CAAX-IRES2-BphP1- iB(GFP)- IRES2-NSImb-NES-mCh-Q-PAS1-NotI

Available Since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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EGFP-miSFIT-12-C

Plasmid

#124693

Purpose

Tuning expression of EGFP

Depositor

Tudor Fulga

Article

Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

Insert

EGFP

Expression

Mammalian

Promoter

minimal CMV

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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EGFP-miSFIT-9-G

Plasmid

#124692

Purpose

Tuning expression of EGFP

Depositor

Tudor Fulga

Article

Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

Insert

EGFP

Expression

Mammalian

Promoter

minimal CMV

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge