We narrowed to 4,801 results for: PIR

1. pMetTU1-A_Sv5K_araC-PBAD_MCD1_MegaB-S9G-R31L-R85L_MegaRNFGLSKJTU_Bba-B0015

   Plasmid

   #202025

   Purpose

   Expresses Bacillus megaterium ARG cluster (GvpB S9G-R31L-R85L mutant) in E. coli

   Depositor

   Mikhail Shapiro

   Article

   Hurt et al bioRxiv 2024.03.30.587094

   Insert

   Bacillus megaterium ARG cluster (GvpB S9G-R31L-R85L mutant)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   GvpB S9G-R31L-R85L

   Promoter

   Available since

   July 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. E17TAG GFP zeo resistance

   Plasmid

   #69118

   Purpose

   For E17TAG GFP reporter expression in non-zeocin resistant strains

   Depositor

   Jesse Rinehart

   Article

   Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

   Insert

   E17TAG GFP
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   E17 to TAG

   Promoter

   Available since

   Sept. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

3. Beta lactamase S68TAG

   Plasmid

   #68305

   Purpose

   Beta lactamase S68TAG gene

   Depositor

   Jesse Rinehart

   Article

   Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

   Insert

   S68TAG beta lactamase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28_LmFPPS

   Plasmid

   #186322

   Purpose

   pET-28a vector (Novagen) (encoding an N-terminal His tag), to transform Escherichia coli BL21 (DE3) cells

   Depositor

   Sandra Gabelli

   Article

   Aripirala et al Acta Crystallogr D Biol Crystallogr. 201

   Insert

   FPPS
   

   Use

   Tags

   His Tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTcI39-PRM-wasabi

   Plasmid

   #195555

   Purpose

   Temperature-dependent expression of mWasabi reporter with transcription activation by TcI39

   Depositor

   Mikhail Shapiro

   Article

   Xiong et al ACS Synth Biol. 2022 Jul 15;11(7):2518-2

   Inserts

   mWasabi
   TcI39
   

   Use

   Tags

   6x His and AANDENYADAS degradation tag

   Expression

   Bacterial

   Mutation

   A67T

   Promoter

   Available since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMetTU1-A_Sv5K_araC-PBAD_MCD1_AnaA-T6A-L40A_AnaCNJKFGW_Bba-B0015

   Plasmid

   #202024

   Purpose

   Expresses Anabaena flos-aquae ARG cluster (GvpA T6A-L40A mutant) in E. coli

   Depositor

   Mikhail Shapiro

   Article

   Hurt et al bioRxiv 2024.03.30.587094

   Insert

   Anabaena flos-aquae ARG cluster (GvpA T6A-L40A mutant)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   GvpA T6A-L40A

   Promoter

   Available since

   Aug. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAP01

   Plasmid

   #186260

   Purpose

   sfGFP under light light responsive PEL222 promoter

   Depositor

   Yongku Cho

   Article

   Pirhanov et al ACS Synth Biol. 2021 Feb 19;10(2):345-35

   Insert

   sfGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PEL222 (GGTAGCCTTTAGTCCATGTTAGCGAAGAAAATGGTTTGTTA…

   Available since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1_GEM7-Halo-FRB

   Plasmid

   #197058

   Purpose

   Expression of Rhodospirillum rubrum encapsulin Halo-FRB-fusion in mammalian cells

   Depositor

   Julia Mahamid

   Article

   Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

   Insert

   Encapsulin
   

   Use

   Tags

   FRB and HaloTag

   Expression

   Mammalian

   Mutation

   Codon-optimised for human cell expression

   Promoter

   Available since

   Sept. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEF1alpha-rtTA-Antares

   Plasmid

   #197587

   Purpose

   Expresses TET-On reverse transactivator labeled with Antares

   Depositor

   Mikhail Shapiro

   Article

   Hurt et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   rtTA-P2A-Antares
   

   Use

   Tags

   Antares

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1alpha

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. SQT1665

    Plasmid

    #78744

    Purpose

    Human expression plasmid for LbCpf1-NLS-3xHA

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

    Insert

    mammalian codon-optimized Lachnospiraceae bacterium ND2006 Cpf1-NLS-3xHA
    

    Use

    CRISPR

    Tags

    NLS-3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGBW-m4046785

    Plasmid

    #145684

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 N (nucleocapsid)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 N (nucleocapsid) (N Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;StrepII

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pJK-caCas9-NatMX-Neut5L

    Plasmid

    #89576

    Purpose

    caCas9 integrating vector into the Neut5L locus

    Depositor

    George Church

    Article

    Shapiro et al Nat Microbiol. 2018 Jan;3(1):73-82. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHYP-NPC-10H

    Plasmid

    #162789

    Purpose

    SARS-CoV-2 Nucleocapsid Protein expression in E.coli

    Depositor

    Ivan Vorobiev

    Article

    Kolesov et al PeerJ. 2022 Jan 7;10:e12751. doi: 10.771

    Insert

    Nucleocapsid phosphoprotein [Severe acute respiratory syndrome coronavirus 2] (N Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    10xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7lac

    Available since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMA-Thermal_CTLA-4

    Plasmid

    #199751

    Purpose

    Thermal expression of CTLA-4

    Depositor

    Mikhail Shapiro

    Article

    Abedi et al Nat Commun. 2022 Mar 24;13(1):1585. doi:

    Insert

    CTLA-4 (Ctla4 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBEST_OR2-OR1-Pr-UTR1_ClpP-T500

    Plasmid

    #153302

    Purpose

    Expresses ClpP protease in the cell-free TX-TL system

    Depositor

    Mikhail Shapiro

    Article

    Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

    Insert

    clpP (clpP Escherichia coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGBW-m4046810

    Plasmid

    #149251

    Purpose

    Bacterial expression plasmid for SARS-CoV-2 N (nucleocapsid)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 N (nucleocapsid) (N Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;HA

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    May 11, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pET28a-T5-ASG_ClpXP

    Plasmid

    #153299

    Purpose

    Expresses acoustic sensor genes or ClpXP-sensitive gas vesicles in E. coli Nissle 1917

    Depositor

    Mikhail Shapiro

    Article

    Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

    Insert

    ASG
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTetR-BCD2-Ana GvpC

    Plasmid

    #153298

    Purpose

    Expresses wild-type gas vesicle protein C (GvpC) from Anabaena flos-aquae

    Depositor

    Mikhail Shapiro

    Article

    Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

    Insert

    Gas vesicle protein C
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pTet

    Available since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGG-PIP31

    Plasmid

    #196769

    Purpose

    Contains RBOHD promoter

    Depositor

    Marc Somssich

    Article

    Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

    Insert

    RESPIRATORY BURST OXIDASE HOMOLOGUE D (RBOHD Mustard Weed)
    

    Use

    Greengate (gg) entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTcI38-PRM-wasabi

    Plasmid

    #195554

    Purpose

    Temperature-dependent expression of mWasabi reporter with transcription activation by TcI38

    Depositor

    Mikhail Shapiro

    Article

    Xiong et al ACS Synth Biol. 2022 Jul 15;11(7):2518-2

    Inserts

    mWasabi
    TcI38
    

    Use

    Tags

    6x His and AANDENYADAS degradation tag

    Expression

    Bacterial

    Mutation

    A67T, M1V, L65S, K68R, F115L, D126G, D188G

    Promoter

    Available since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge