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TC374

Plasmid

#164886

Purpose

Prpl28::rtetR-QFAD::p2A::mKate::T2A::tetR-pie1,

Depositor

Tian Chi

Article

Mao et al iScience. 2019 Jan 25;11:224-237. doi: 1

Insert

rpl28-rtTA(Q)

Expression

Worm

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pAM1954

Plasmid

#40250

Depositor

Susan Golden

Article

Yoon et al Science. 1998 Oct 30;282(5390):935-8.

Insert

GFPmut2

Use

Cyanobacteria cloning vector

Promoter

n/a

Available Since

March 25, 2014

Availability

Academic Institutions and Nonprofits only

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pAM1956

Plasmid

#40251

Depositor

Susan Golden

Article

Yoon et al Science. 1998 Oct 30;282(5390):935-8.

Insert

GFPmut2

Use

Cyanobacteria cloning vector

Promoter

n/a

Available Since

March 25, 2014

Availability

Academic Institutions and Nonprofits only

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pCM5.40

Plasmid

#19393

Depositor

Geraldine Seydoux

Article

Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

Insert

glp-1 3'utr in pDONRP2RP3 (glp-1 Nematode)

Expression

Worm

Available Since

Nov. 10, 2008

Availability

Academic Institutions and Nonprofits only

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CH00X

Plasmid

#73517

Purpose

TagRFP-T_PH pCFJ150 - contains C. elegans codon optimized TagRFP-T

Depositor

Bob Goldstein

Article

Heppert et al Mol Biol Cell. 2016 Nov 7;27(22):3385-33

Insert

pmex-5::TagRFP-T::PH::tbb-2

Tags

TagRFP-T

Promoter

mes-5

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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JH04

Plasmid

#73513

Purpose

mRuby2_PH pCFJ150 - contains C. elegans codon optimized mRuby2

Depositor

Bob Goldstein

Article

Heppert et al Mol Biol Cell. 2016 Nov 7;27(22):3385-33

Insert

pmex-5::mRuby2::PH::tbb-2

Tags

mRuby2

Promoter

mex-5

Available Since

Feb. 29, 2016

Availability

Academic Institutions and Nonprofits only

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peft-3::cas-9::tbb-2 3'UTR

Plasmid

#48960

Purpose

this plasmid contains the codon optimized gene encoding Cas9 for C. elegans.

Depositor

Mario de Bono

Article

Chen et al Nucleic Acids Res. 2013 Sep 5.

Insert

cas9

Use

CRISPR

Promoter

eef-1A.1 (eft-3)

Available Since

April 17, 2014

Availability

Academic Institutions and Nonprofits only

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pGENE:EFF-1::mCherry::V5

Plasmid

#133020

Purpose

EFF-1 fused to mCherry fluorescent protein cloned into the pGene vector for inducible expression in mammalian cells

Depositor

Benjamin Podbilewicz

Article

Perez-Vargas et al Cell. 2014 Apr 10;157(2):407-419. doi: 1

Insert

EFF-1::mCherry::V5

Tags

V5; mCherry

Expression

Mammalian

Available Since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pSF11-wmiRFP680-T2A-HO1

Plasmid

#197244

Purpose

Expresses the protein of wmiRFP680-T2A-HO1 in neurons of C elegan

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

wmiRFP680-T2A-HO1

Expression

Worm

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pAAP2

Plasmid

#171208

Purpose

bacterial expression for purification of PGL-3, allowing cleavage for MBP tag using TEV protease

Depositor

Geraldine Seydoux

Article

Schmidt et al bioRxiv 2020.10.15.340570

Insert

pgl-3 (pgl-3 Nematode)

Tags

MBP::HIS::TEV cleavage site

Expression

Bacterial

Available Since

June 23, 2021

Availability

Academic Institutions and Nonprofits only

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EFF-1::V5

Plasmid

#132961

Purpose

Expresses EFF-1 A full length protein in mammalian cells

Depositor

Benjamin Podbilewicz

Article

Valansi et al J Cell Biol. 2017 Mar 6;216(3):571-581.

Insert

EFF-1::V5

Tags

V5

Expression

Mammalian

Available Since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pAP974-1

Plasmid

#99497

Purpose

TEV::linker::tagRFP::3XFlag

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

TEV::linker::tagRFP::3XFlag

Expression

Bacterial

Available Since

Oct. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pHCMM4

Plasmid

#170359

Purpose

Expresses a Sec61p-mCherry ER-marker from the CYC1 promoter

Depositor

Hugo Cesar Medina-Munoz, Marvin Wickens

Article

Medina-Munoz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

Insert

PCYC1_SEC61_mCherry_LEU2_TCYC1

Expression

Yeast

Promoter

PCYC1

Available Since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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pRG02

Plasmid

#44196

Depositor

Denis Dupuy

Insert

Neo

Expression

Worm

Promoter

rps-27

Available Since

April 10, 2013

Availability

Academic Institutions and Nonprofits only

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pAS1-rab-3P::his24::tagBFP2

Plasmid

#124344

Purpose

Nuclear Localized Pan-Neuronal tagBFP2

Depositor

Andrew Leifer

Insert

rab3, tagBFP2 (rab-3 Nematode)

Expression

Worm

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pKS2301

Plasmid

#37834

Depositor

Gary Silverman

Article

Miedel et al PLoS One. 2012;7(7):e40145. Epub 2012 Ju

Insert

Pnhx-2cpl-1::YFP (cpl-1 Nematode)

Tags

YFP

Expression

Worm

Promoter

nhx-2

Available Since

Dec. 10, 2012

Availability

Academic Institutions and Nonprofits only

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(p)odr-3::GFP::EGL-4

Plasmid

#100897

Purpose

GFP and the EGL-4 cDNA driven by the odr-3 promoter, which expresses in the AWA, AWB, and AWC olfactory sensory neurons in C. elegans. unc-54 3'UTR in sequence as well.

Depositor

Noelle L'Etoile

Article

Lee et al Proc Natl Acad Sci U S A. 2010 Mar 30;10

Insert

56-2741: odr-3 promoter

Expression

Worm

Promoter

odr-3

Available Since

Sept. 15, 2017

Availability

Academic Institutions and Nonprofits only

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pJW6.01

Plasmid

#62882

Purpose

Transgene plasmid for expressing GFP::MEG-3 under meg-3 promoter and 3'UTR

Depositor

Geraldine Seydoux

Article

Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

Insert

GFP::MEG-3

Tags

GFP

Expression

Worm

Promoter

meg-3

Available Since

March 30, 2015

Availability

Academic Institutions and Nonprofits only

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mg180

Plasmid

#34660

Depositor

Miriam Goodman

Article

Johnson et al Proc Natl Acad Sci U S A. 2011 Nov 2.

Insert

slo-1(A2;B0;C1) (slo-1 Nematode)

Use

Xenopus oocyte expression

Tags

Xenopus beta-globulin 3' UTR and Xenopus bet…

Available Since

Feb. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pSEM237 - pEXP[Prpl-3 | histamine | rpl-3 UTR]

Plasmid

#159796

Purpose

Histamine based negative selection marker to paralyze animals with arrays

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al MicroPubl Biol. 2021 Jan 8;2021. doi: 10

Insert

pEXP[Prpl-3 | histamine | rpl-3 UTR]

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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