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We narrowed to 2,368 results for: pcas9

Showing: 521 - 540 of 2368 results

  1. pET-FLAG-SpCas9-HF1-plus

    Plasmid
    #126775
    Purpose
    Expression of increased fidelity SpCas9-HF1-plus in bacterial cells. Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as SpCas9-HF1.
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    SpCas9-HF1-plus
    Use
    CRISPR
    Tags
    3xFLAG, 6xHis, MBP, and NLS
    Expression
    Bacterial
    Mutation
    N497A; Q695A; Q926A; amino acids 1005-1013 replac…
    Promoter
    T7
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126775-plasmid-map-sequence-id-437774

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  2. pspCas9-POLI-ST2-com vector

    Plasmid
    #176234
    Purpose
    Vector plasmid for expressing spCas9-POLI fusion protein and sgRNA. There are two copies of HBB 3’ UTR in the 3’UTR of spCas9-POLI to enhance expression. The ST2 loop of the sgRNA scaffold was replace
    Depositor
    Baisong Lu
    Article
    Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    March 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    176234-plasmid-map-sequence-id-348845

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  12. AAV-pCamKII-Cre-pU6-SpCas9gRNAentry

    Plasmid
    #210391
    Purpose
    AAV plasmid encoding the CamKII promoter driving Cre expression, along with SpCas9 gRNA entry cassette (RFP dropout)
    Depositor
    Benjamin Kleinstiver
    Article
    Huynh et al Nature. 2024 Nov;635(8037):168-177. doi:
    Insert
    AAV-(ITR)-pCamKII-NLS-Cre-WPRE-bGHpA-(rev)-SpCas9_gRNA-BsmBI_RFPcassette-hU6-(ITR) (NJA158)
    Use
    AAV and CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CamKII promoter for Cre, human U6 promoter for Sp…
    Available Since
    July 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    210391-plasmid-map-sequence-id-415458

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  15. pX330-U6-BRD4-HaloTag-Guide-hspCas9

    Plasmid
    #228576
    Purpose
    Expression of Halo-tagged human BRD4 gRNA; CRISPR-mediated gene insertion
    Depositor
    Marcin Imielinski
    Insert
    BRD4 gRNA (BRD4 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Jan. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    228576-plasmid-map-sequence-id-457355

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Showing: 521 - 540 of 2368 results