We narrowed to 12,452 results for: SOM

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pFB1.HMBP.PrS.EZH2-mt1

Plasmid

#220239

Purpose

Expression of an EZH2 mutant in insect cells.

Depositor

Chen Davidovich

Article

Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

Insert

EZH2 mt 1 (EZH2 Human)

Tags

6x His, MBP

Expression

Insect

Mutation

R27A, R31A, F165A

Promoter

Polyhedrin

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3-TEX264-SIM1_mut-cFlag

Plasmid

#220205

Purpose

Mammalian expression of TEX264 with SIM1 motif mutated, C-terminal Flag

Depositor

Kristijan Ramadan

Article

Fielden et al Nat Commun. 2020 Mar 9;11(1):1274. doi:

Insert

TEX264 (TEX264 Human)

Tags

Flag

Expression

Mammalian

Mutation

p.111LIDLY>AAAAA; c.331C>G,332T>C,334A&g…

Promoter

CMV

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA5-TEX264-SIM1_mut-cGFP

Plasmid

#220212

Purpose

Mammalian expression of TEX264 with SIM1 motif mutated, fused at C-terminus to GFP

Depositor

Kristijan Ramadan

Article

Fielden et al Nat Commun. 2020 Mar 9;11(1):1274. doi:

Insert

TEX264 (TEX264 Human)

Tags

GFP

Expression

Mammalian

Mutation

p.111LIDLY>AAAAA; c.331C>G,332T>C,334A&g…

Promoter

CMV/TetO

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO GABARAP(G116A)-mCherry-GABARAP

Plasmid

#212113

Purpose

Expression of GABARAP split tandem protein with mCherry

Depositor

Dieter Willbold

Article

Uffing et al Autophagy Rep. 2024 May 16;3(1):2348899.

Insert

GABARAP(G116A)-mCherry-GABARAP (GABARAP Discosoma sp., Human)

Tags

mCherry

Expression

Mammalian

Mutation

G116A (lipidation deficiency) of upstream GABARAP

Promoter

CMV

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-mOrange/CALR ins5

Plasmid

#214769

Purpose

Mammalian expression of human CALR ins5

Depositor

Norio Komatsu

Article

De Marchi et al Leuk Res. 2022 Aug;119:106883. doi: 10.1

Insert

human CALR ins5 (CALR Human)

Use

Retroviral

Promoter

MSCV

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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Lenti-sgRNA-Cre-GpNLuc-sgmMRE11

Plasmid

#208384

Purpose

Lentiviral vector expressing Cas9, GpNLuc, and sgRNA targeting murine MRE11 gene.

Depositor

Gaorav Gupta

Article

Cho et al Nature. 2024 Jan 10. doi: 10.1038/s41586

Insert

sgMRE11 (Mre11a Mouse)

Use

Lentiviral and Luciferase

Expression

Mammalian

Mutation

WT

Available Since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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Lenti-sgRNA-Cre-GpNLuc-sgmZBP1#3

Plasmid

#208389

Purpose

Lentiviral vector expressing Cas9, GpNLuc, and sgRNA targeting murine ZBP1#3 gene.

Depositor

Gaorav Gupta

Article

Cho et al Nature. 2024 Jan 10. doi: 10.1038/s41586

Insert

sgZBP1 (Zbp1 Mouse)

Use

Lentiviral and Luciferase

Expression

Mammalian

Mutation

WT

Available Since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_PICK1_POLR2F

Plasmid

#205894

Purpose

Express mEGFP-tagged fusion protein, PICK1_POLR2F from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

PICK1_POLR2F (PICK1, POLR2F Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_DCAF15_ZNF333

Plasmid

#205801

Purpose

Express mEGFP-tagged fusion protein, DCAF15_ZNF333 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

DCAF15_ZNF333 (DCAF15, ZNF333 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pETM30-C9ORF78

Plasmid

#193367

Purpose

Expression of C9ORF78 in E.coli with N-terminal His6-GST-tag which is cleavable with TEV protease

Depositor

Markus Wahl

Article

Bergfort et al Nat Commun. 2022 Mar 3;13(1):1132. doi:

Insert

C9ORF78 (C9orf78 Human)

Tags

His6-GST-TEV

Expression

Bacterial

Promoter

T7

Available Since

June 7, 2023

Availability

Academic Institutions and Nonprofits only

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Halo-H1DBD-H2

Plasmid

#200205

Purpose

Expression of HSF2 with HSF1 DBD

Depositor

Sheila Teves

Article

Price et al Nucleic Acids Res. 2023 Apr 28:gkad304.

Insert

Halo-H1DBD-H2 (Hsf1, Hsf2 Mouse)

Tags

Halo

Expression

Mammalian

Mutation

HSF1 (1-399); HSF2 (366-1554)

Promoter

EF_1alpha

Available Since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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H2DBD-H1-Halo

Plasmid

#200193

Purpose

Expression of HSF1 with HSF2 DBD

Depositor

Sheila Teves

Article

Price et al Nucleic Acids Res. 2023 Apr 28:gkad304.

Insert

H2DBD-H1-Halo (Hsf1, Hsf2 Mouse)

Tags

Halo

Expression

Mammalian

Mutation

HSF2 (1-366); HSF1 (399-1576)

Promoter

EF_1alpha

Available Since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-DIO-inv[roxSTOProx-Lyn-GFP-bGH]

Plasmid

#196884

Purpose

Dual Cre/Dre-dependent expression of LynGFP. Used as Cre-Dre cotransfection reporter

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

roxSTOProx-Lyn-GFP-bGH

Use

Cre/Lox; Dre/rox

Expression

Mammalian

Promoter

Quimeric CAG

Available Since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pBactin-DIO-inv[roxSTOProx-ZsGreen-bGH]

Plasmid

#196886

Purpose

Dual Cre/Dre-dependent expression of ZsGreen. Used as Cre-Dre cotransfection reporter

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

roxSTOProx-ZsGreen-bGH

Use

Cre/Lox; Dre/rox

Expression

Mammalian

Promoter

Chicken bactin (plus Chicken bactin intron)

Available Since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-myc Metap2

Plasmid

#199685

Purpose

Transient expression of Metap2

Depositor

Roberto Zoncu

Article

Shin et al Science. 2022 Sep 16;377(6612):1290-1298

Insert

METAP2 (METAP2 Human)

Tags

myc

Expression

Mammalian

Promoter

CMV

Available Since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

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pGBT9-ATG9A-N

Plasmid

#198530

Purpose

Expression of GAL4 DNA-binding domain (BD)-ATG9A-N terminal cytosolic domain fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Mattera et al Proc Natl Acad Sci U S A. 2017 Dec 12;11

Insert

ATG9A N-terminal cytosolic tail (residues 1-66) (ATG9A Human)

Tags

GAL4-DNA binding domain fragment

Expression

Yeast

Promoter

ADH1

Available Since

April 3, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsAGO2-K663AL697Y_AC

Plasmid

#148427

Purpose

Mammalian Expression of HsAGO2-K663AL697Y

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

HsAGO2-K663AL697Y (AGO2 Human)

Expression

Mammalian

Mutation

three silent mutations and the sequence differs i…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsAGO2-P593GA623FK663AL697Y_AC

Plasmid

#148428

Purpose

Mammalian Expression of HsAGO2-P593GA623FK663AL697Y

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

HsAGO2-P593GA623FK663AL697Y (AGO2 Human)

Expression

Mammalian

Mutation

three silent mutations and the sequence differs i…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-NDUFA10-mVenus

Plasmid

#185648

Purpose

NDUFA10 Complex I sub-unit with C-terminus mVenus tag

Depositor

Dipayan Chaudhuri

Article

Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

Insert

NDUFA10 (NDUFA10 Human)

Tags

mVenus

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-TAPtagN-PrP-SBP-HsAGO2_AC

Plasmid

#148440

Purpose

Mammalian Expression of HsAGO2

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

HsAGO2 (AGO2 Human)

Expression

Mammalian

Mutation

one silent mutation C840Tand sequence differs in …

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-NDUFB6-mVenus

Plasmid

#185652

Purpose

NDUFB6 Complex I sub-unit with C-terminus mVenus tag

Depositor

Dipayan Chaudhuri

Article

Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

Insert

NDUFB6 (NDUFB6 Human)

Tags

mVenus

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-NDUFS3-mVenus

Plasmid

#185649

Purpose

NDUFS3 Complex I sub-unit with C-terminus mVenus tag

Depositor

Dipayan Chaudhuri

Article

Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

Insert

NDUFS3 (NDUFS3 Human)

Tags

mVenus

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

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fru-T2A-CsChrimson-tdTomato HDR plasmid

Plasmid

#141100

Purpose

Plasmid for CRISPR-generated knock-in line that expresses optogenetic activator CsChrimson-tdTomato by knocking-in fragment into the coding sequence of the endogenous Aedes aegypti fru gene

Depositor

Leslie Vosshall

Article

Basrur et al Elife. 2020 Dec 7;9. pii: 63982. doi: 10

Insert

fru-left-HDR-arm; T2A-CsChrimson-tdTomato-SV40; 3xP3-EYFP-SV40; fru-right-HDR-arm

Use

Donor template

Available Since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only

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Cas9_ANKRD1_sgRNA2

Plasmid

#186669

Purpose

Plasmid containing Cas9 and ANKRD1 sgRNA2 , sgRNA sequence: cggtcagcttatatagct.

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

ANKRD1 KO sgRNA Plasmid (ANKRD1 Human)

Expression

Mammalian

Available Since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-NDUFA2-mVenus

Plasmid

#185645

Purpose

NDUFA2 Complex I sub-unit with C-terminus mVenus tag

Depositor

Dipayan Chaudhuri

Article

Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

Insert

NDUFA2 (NDUFA2 Human)

Tags

mVenus

Expression

Mammalian

Promoter

CMV

Available Since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-NDUFA10-FKBP-HA

Plasmid

#185810

Purpose

NDUFA10 subunit of Complex I fused with C-terminal FKBP and HA epitope

Depositor

Dipayan Chaudhuri

Article

Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

Insert

NDUFA10 (NDUFA10 Human)

Use

Lentiviral

Tags

FKBP and HA

Expression

Mammalian

Promoter

CMV

Available Since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

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pT22gcfUbm5En25EE4PCh(#552)

Plasmid

#184092

Purpose

cyclofen-inducible chicken EN2 (mut) activation in zebrafish permanent transgenic

Depositor

Sophie Vriz

Article

Amblard et al Commun Biol. 2020 Sep 29;3(1):536. doi:

Insert

5myc-chkEn2(5E)-ERT2-P2A-mCherry

Use

Zebrafish transgenesis (blue eyes)

Tags

5xMyc

Mutation

chkEn2: S150E, S151E, S153E, S155E, S156E; ERT2: …

Promoter

Zf-Ubi

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pT22Ubm4Eng2bKRPC(#493)

Plasmid

#184091

Purpose

cyclofen-inducible fish Eng2b (mut) activation in zebrafish permanent transgenic

Depositor

Sophie Vriz

Article

Amblard et al Commun Biol. 2020 Sep 29;3(1):536. doi:

Insert

4myc-ZfEng2b(WW>KK)-ERT2-P2A-mCherry

Use

Zebrafish transgenesis (blue eyes)

Tags

4xMyc

Mutation

ZfEng2b: W141K, W144K; ERT2: G400V, M543A, L544A,…

Promoter

Zf-Ubi

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pT22UbGfpnE4PCh(#301)

Plasmid

#184066

Purpose

cyclofen-inducible GFP nuclear relocation in zebrafish permanent transgenic

Depositor

Sophie Vriz

Article

Zhang et al Chembiochem. 2018 Jun 18;19(12):1232-123

Insert

eGFP-nls-ERT2-P2A-mCherry

Use

Zebrafish transgenesis (blue eyes)

Mutation

ERT2: G400V, M543A, L544A, deleted 1-281;

Promoter

Zf-Ubi

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pBoBi-hPEN2 20A

Plasmid

#183639

Purpose

Lentiviral expression of a PEN2 mutant showing impaired affinity with ATP6AP1.

Depositor

Sheng-cai Lin

Article

Ma et al Nature. 2022 Mar;603(7899):159-165. doi:

Insert

PEN2 (PSENEN Human)

Use

Lentiviral

Tags

HA

Mutation

residues 27, 28, 30, 31, 34, 38, 42, 43, 57, 58, …

Promoter

CMV

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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