Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 24,939 results for: SPR

Clear keywords
Showing: 10761 - 10800 of 24939 results

  1. qTAG-C-miRFP670nano3-Blast-H2BC11

    Plasmid
    #207762
    Purpose
    Donor template for miRFP670nano3-2A-Blast insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    H2BC11 Homology Arms flanking a miRFP670nano3-Blast Cassette (H2BC11 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Available Since
    Nov. 29, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207762-plasmid-map-sequence-id-416207

    Enlarge

  2. Lenti-sgRNA-Cre-GpNLuc

    Plasmid
    #208382
    Purpose
    Derived from LentiCRISPRV2 by replacing Cas9 with Cre recombinase and puromycin resistance with GpNLuc. Contains a stuffer sequence for cloning of sgRNA of interest, as per LentiCRISPRV2.
    Depositor
    Gaorav Gupta
    Article
    Cho et al Nature. 2024 Jan 10. doi: 10.1038/s41586
    Type
    Empty backbone
    Use
    Lentiviral and Luciferase
    Expression
    Mammalian
    Mutation
    WT
    Available Since
    Jan. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    208382-plasmid-map-sequence-id-412369

    Enlarge

  6. qTAG-AAVS1-PGK-Puro-moxGFP

    Plasmid
    #227275
    Purpose
    AAVS1 targeting donor for the insertion of Puro and a medium strength PGK expressing moxGFP. To be co-transfected with sgRNA plasmid px330-AAVS1 (Addgene #227272)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    AAVS1 Homology Arms flanking a 2A-Puro-PGK-moxGFP cassette (AAVS1 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227275-plasmid-map-sequence-id-452039

    Enlarge

  7. pCAG-memRFP-3xPax7gRNA

    Plasmid
    #224569
    Purpose
    Ubiquitous expression plasmid, CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), three unique Pax7 gRNAs with ribozyme self-cleavage sites, and membrane RFP reporter.
    Depositor
    Erica Hutchins
    Article
    Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026
    Insert
    mRFP1
    Use
    CRISPR
    Tags
    Membrane localization signal
    Mutation
    Codon optimized
    Available Since
    Dec. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    224569-plasmid-map-sequence-id-451497

    Enlarge

  12. pDIV537

    Plasmid
    #177704
    Purpose
    Plasmid expressing optimized Cas9 and NAT marker and sgRNA targeting KU70 homolog in D. hansenii
    Depositor
    Uffe Mortensen
    Article
    Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0
    Inserts
    Cas9
    Nat
    tRNA-sgRNA-tRNA
    Use
    CRISPR
    Tags
    SV40
    Expression
    Bacterial and Yeast
    Promoter
    RNR2p (Debaryomyces hansenii ), TDH3p (Candida lu…
    Available Since
    March 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    177704-plasmid-map-sequence-id-352590

    Enlarge

  14. qTAG-C-moxGFP-Zeo-H2BC11

    Plasmid
    #207759
    Purpose
    Donor template for moxGFP-2A-Zeo insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    H2BC11 Homology Arms flanking a moxGFP-Zeo Cassette (H2BC11 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Available Since
    Nov. 17, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207759-plasmid-map-sequence-id-415319

    Enlarge

  15. pUC57-white[coffee]

    Plasmid
    #84006
    Purpose
    Donor HR template carrying a 2,080 bp fragment of the Drosophila melanogaster white[coffee] allele and silent mutations conferring resistance to white sgRNAs-1, -2, -3, and -4
    Depositor
    Phillip Zamore
    Article
    Ge et al G3 (Bethesda). 2016 Aug 26. pii: g3.116.
    Insert
    A 2,080 bp fragment of the white[coffee] allele (w Fly)
    Use
    Unspecified
    Mutation
    A GC-to-AA mutation that creates a G589E missense…
    Available Since
    Nov. 8, 2016
    Availability
    Academic Institutions and Nonprofits only
    84006-plasmid-map-sequence-id-165529

    Enlarge

  16. pCAT105

    Plasmid
    #180510
    Purpose
    Plasmid expressing mammalian codon optimized engineered DpbCasX-R3, sgRNAv2 scaffold, and restriction sites to clone in new spacers
    Depositor
    Jennifer Doudna
    Article
    Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6
    Inserts
    CasX sgRNAv2
    DpbCasX with R3 loop
    Use
    CRISPR
    Tags
    2x FLAG and SV40 NLS
    Expression
    Mammalian
    Promoter
    CAG and U6
    Available Since
    April 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    180510-plasmid-map-sequence-id-359734

    Enlarge

  21. LentiRCas9-Lambda2

    Plasmid
    #104184
    Purpose
    Lentiviral transfer vector that carries U6-driven non-targeting sgRNA using a modified scaffold (Chen et al. Cell 2013) and CMV-driven PIN-dCas9. Derived from LentiCRISPR v2 (Zhang lab)
    Depositor
    Eugene Yeo
    Article
    Batra et al Cell. 2017 Aug 24;170(5):899-912.e10. do
    Insert
    U6-sgRNA, EFS-PIN-dCas9
    Use
    CRISPR and Lentiviral
    Available Since
    April 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    104184-plasmid-map-sequence-id-201745

    Enlarge

  22. pCMV-T7-AsCas12a-P2A-EGFP (RTW2861)

    Plasmid
    #160140
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized AsCas12a with a c-terminal NLS(nucleoplasmin), 3x HA tag, and P2A-EGFP
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41
    Insert
    human codon optimized AsCas12a with NLS(nucleoplasmin)-3xHA-P2A-EGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    NLS(nucleoplasmin)-3xHA-P2A-EGFP
    Expression
    Mammalian
    Promoter
    CMV and T7
    Available Since
    Feb. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    160140-plasmid-map-sequence-id-317331

    Enlarge

  25. qTAG-N-Solo-mStayGold-MYO1C

    Plasmid
    #227306
    Purpose
    Donor template for mStayGold insertion into the N-terminus of the MYO1C locus. For membrane visualization. To be co-transfected with sgRNA plasmid px330-PITCh-MYO1C (Addgene #227305)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    MYO1C Homology Arms flanking a mStayGold Tag (MYO1C Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227306-plasmid-map-sequence-id-452076

    Enlarge

  35. AA145

    Plasmid
    #215937
    Purpose
    Fragmid fragment: (guide cassette) guide expression; Chen F+E trRNA (preferred)
    Depositor
    John Doench
    Article
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Has Service
    Cloning Grade DNA
    Insert
    U6_v1; BsmBI_v0; BsmBI_v1; trRNA_v4 [Sp]
    Use
    CRISPR; Fragment
    Available Since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    215937-plasmid-map-sequence-id-431395

    Enlarge

  38. pPPC011

    Plasmid
    #171143
    Purpose
    Expression of Sp.pCas9-dCas9 and BBa_J23107-MCP-SoxS(R93A/S101A) on pRK2-KmR plasmid
    Depositor
    Jesse Zalatan
    Article
    Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(
    Insert
    Sp.pCas9-dCas9_BBa_J23107-MCP-SoxS(R93A, S101A)
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    SoxS has R93A and S101A mutations
    Promoter
    Sp.pCas9, BBa_J23107
    Available Since
    Oct. 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    171143-plasmid-map-sequence-id-345216

    Enlarge

  40. pET28b-BE3∆UGI

    Plasmid
    #96943
    Purpose
    The plasmid encoding the His6-rAPOBEC1-XTEN-nCas9 protein (BE3∆UGI) was generated by site-directed mutagenesis using pET28b-BE1 (Addgene plasmid #73018).
    Depositor
    Daesik Kim
    Article
    Kim et al Nat Biotechnol. 2017 May;35(5):475-480.
    Insert
    BE3∆UGI
    Tags
    His6 tag
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Sept. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    96943-plasmid-map-sequence-id-193667

    Enlarge

Showing: 10761 - 10800 of 24939 results