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We narrowed to 30,195 results for: REP

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Showing: 5401 - 5420 of 30195 results

  3. pPV-TetO-SpCas9-GR-iC-ERiP (CRONUS-Puro)

    Plasmid
    #100596
    Purpose
    piggyBac vector expressing Dual-regulated Cas9 (Puro resistance)
    Depositor
    Akitsu Hotta
    Article
    Ishida et al Sci Rep. 2018 Jan 10;8(1):310. doi: 10.1
    Inserts
    CRISPR Cas9 fused with human Glucocorticoid Receptor
    mCherry
    rtTA-M2
    Use
    Piggybac vector
    Expression
    Mammalian
    Mutation
    Codon-optimized for human codon usage
    Promoter
    Dox-inducible TetO promoter and Human EEF1A1 prom…
    Available Since
    April 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    100596-plasmid-map-sequence-id-207339

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  6. pICE-HA-Ku70-siR-Mut6E

    Plasmid
    #82330
    Purpose
    Plasmid for constitutive or doxy-inducible expression of mutant human Ku70 unable to bind DNA and resistant to siRNA. Confers resistance to puro. Use T-REx cells for doxycycline-inducible expression.
    Depositor
    Sébastien Britton, Patrick Calsou
    Article
    Lamaa et al EMBO Rep. 2016 Apr;17(4):508-18. doi: 10
    Insert
    Ku70 (XRCC6 Human)
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Mut6E corresponding to K282E K287E T300E K331E K3…
    Promoter
    CMV-tet
    Available Since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    82330-plasmid-map-sequence-id-155730

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  9. pMIA3

    Plasmid
    #109399
    Purpose
    Expresses sgRNA cassette (BsmBI cloning site) and EF1-A driven eSpCas9 linked via P2A with mRuby2 and dominant negative mtp53 for enhanced survival of hES after DSB
    Depositor
    Roger Foo
    Article
    Ang et al Cell Rep. 2018 Feb 20;22(8):2190-2205. d
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    mRuby2 and mtp53dn
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    109399-plasmid-map-sequence-id-211963

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  11. Epac-S-H30

    Plasmid
    #170337
    Purpose
    CFP(UST)_EPACdDEPCD_Y(UST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Kees Jalink
    Article
    Ponsioen et al EMBO Rep. 2004 Dec . 5(12):1176-80.
    Insert
    CFP(UST)_EPACdDEPCD_Y(UST) (RAPGEF3 Human)
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A …
    Promoter
    CMV
    Available Since
    Oct. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    170337-plasmid-map-sequence-id-344639

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  13. mChF-AIP(TA)

    Plasmid
    #61528
    Purpose
    Expression of human FK506 binding protein 1A (FKBP1A) tagged with mCherry and AIP (with TA mutation)
    Depositor
    Takanari Inoue
    Article
    Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:
    Insert
    FK506 binding protein 1A (FKBP1A Human)
    Tags
    AIP with TA mutation (see comments) and mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 17, 2015
    Availability
    Academic Institutions and Nonprofits only

  16. pRetroX-PTuner DD-M.EcoGII-v5-Centromeric protein C

    Plasmid
    #122085
    Purpose
    Express M.EcoGII fused to CENPC with destabilization domain - inducible
    Depositor
    Laure Crabbe
    Article
    Sobecki et al Cell Rep. 2018 Dec 4;25(10):2891-2903.e5
    Insert
    DD-linker-M.EcoGII-V5-CENPC (CENPC E.coli, Human)
    Use
    Retroviral
    Tags
    V5 tag on Nter of CENPC
    Expression
    Bacterial and Mammalian
    Mutation
    L341F, R930L (see comments below)
    Available Since
    March 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    122085-plasmid-map-sequence-id-238127

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  19. pCAG-FLAG-puro-PTPIP51(1-150_175-470)

    Plasmid
    #170537
    Purpose
    Expresses PTPIP51 FFAT-like motif (151-175) deletion mutant (1-150_176-470) in human HeLa cells after transfection
    Depositor
    Byung Il Lee
    Article
    Yeo et al EMBO Rep. 2021 Jun 4;22(6):e51323. doi:
    Insert
    Protein tyrosine phosphatase interacting protein 51 (RMDN3 Human)
    Tags
    FLAG tag
    Expression
    Mammalian
    Mutation
    deleted amino acids 151-175
    Promoter
    CAG promoter
    Available Since
    Aug. 4, 2021
    Availability
    Academic Institutions and Nonprofits only

  20. Epac-S-H27

    Plasmid
    #170336
    Purpose
    CFP(ST)_EPACdDEP _Y(UST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Kees Jalink
    Article
    Ponsioen et al EMBO Rep. 2004 Dec . 5(12):1176-80.
    Insert
    CFP(ST)_EPACdDEP _Y(UST) (RAPGEF3 Human)
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain
    Promoter
    CMV
    Available Since
    June 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    170336-plasmid-map-sequence-id-335892

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Showing: 5401 - 5420 of 30195 results