We narrowed to 29,602 results for: REP

1. Synaptotagmin-6 scFv [N270/47]

   Plasmid

   #190544

   Purpose

   Mammalian Expression of Synaptotagmin-6 scFV. Derived from hybridoma N270/47.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   Synaptotagmin-6 (Mus musculus) recombinant scFV (Syt6 Mouse)
   

   Use

   Tags

   HA, Sortase, 6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. Lenti-sgNotch2#2/Cre

   Plasmid

   #193226

   Purpose

   Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Notch2 gene

   Depositor

   Julien Sage

   Article

   Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

   Insert

   sgNotch2#2 (Notch2 Mouse)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Dec. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. TrpV1 scFv [N221/17]

   Plasmid

   #182082

   Purpose

   Mammalian Expression of TrpV1 scFv. Derived from hybridoma N221/17.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   recombinant mouse scFv targeting TrpV1 (Rattus norvegicus) (Trpv1 Mouse)
   

   Use

   Tags

   Sortase, His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCC_10 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-KRAB-dCas9NG-NLS-2A-Puro-WPRE

   Plasmid

   #139095

   Purpose

   Expresses human codon-optimized inactive SpCas9-NG fused to a transcriptional repressor KRAB in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.

   Depositor

   Neville Sanjana

   Article

   Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

   Insert

   KRAB-dSpCas9-NG
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A, H840A, L1111R, D1135V,G1218R, E1219F, A1322…

   Promoter

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKLV2-U6gRNA5(Nelfb-g1)-PGKpuroBFP-W

   Plasmid

   #105023

   Purpose

   Lentiviral gRNA plasmid targeting mouse Nelfb , co-expression of TagBFP

   Depositor

   Kosuke Yusa

   Article

   Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

   Insert

   Nelfb (Cobra1 Mouse)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKLV2-U6gRNA5(Nelfb-g2)-PGKpuroBFP-W

   Plasmid

   #105024

   Purpose

   Lentiviral gRNA plasmid targeting mouse Nelfb , co-expression of TagBFP

   Depositor

   Kosuke Yusa

   Article

   Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

   Insert

   Nelfb (Cobra1 Mouse)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. FLAG-FullLength-TET1-CXXC/LLAA

   Plasmid

   #124399

   Purpose

   Full-length mutant TET1 (L897A/L900A), missing zinc finger domain (585-624), used to PiggyBAC rescue the function of TET1/2 DKO cells

   Depositor

   Shaun Cowley

   Article

   Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

   Insert

   FLAG-FullLength-TET1 CXXC zinc finger deletion (585-624) and L897A/L900A mutations (TET1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   CXXC zinc finger deletion (aa 585-624) and L897A/…

   Promoter

   Available since

   May 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSpCas9-Puro HLAG-2B-sgRNA

   Plasmid

   #182552

   Purpose

   Cas9 from S.pyogenes with 2A-Puro, and the 2B-sgRNA to targeting exon 2 of human HLA-G gene

   Depositor

   Santiago Miriuka

   Article

   Palma et al Sci Rep. 2021 Nov 12;11(1):22158. doi: 1

   Insert

   hSpCas9-2A-Puro-HLAG-2B-sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available since

   May 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pME mKate2-dynactin1-811

   Plasmid

   #105971

   Purpose

   entry clone for gateway cloning, p150Glued subunit of dynactin, cofactor of dynein, dominant negative truncation, amino acids 1-811

   Depositor

   Caren Norden

   Article

   Taverna et al Sci Rep. 2016 Feb 16;6:21206. doi: 10.10

   Insert

   dynactin1-811 (DCTN1 Human)
   

   Use

   Tags

   mKate2

   Expression

   Bacterial

   Mutation

   contains only amino acids 1-811

   Promoter

   Available since

   July 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. PB-PE VQR

    Plasmid

    #162797

    Purpose

    All-in-one prime editor piggyBac transposon, VQR variant

    Depositor

    Tobias Cantz

    Article

    Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1

    Insert

    SpCas9_H840A_VQR-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
    

    Use

    CRISPR and Synthetic Biology; Piggybac transposon

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    D1135V, R1335Q, T1337R

    Promoter

    CAG

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA8-Flag-LARS-DELTA-UNE-L

    Plasmid

    #139691

    Purpose

    Expresses N-teminal Flag tagged aa 1- 1064 of LARS1

    Depositor

    Jie Chen

    Article

    Yoon et al Cell Rep. 2016 Aug 9;16(6):1510-1517. do

    Insert

    LARS1 (LARS1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. SIN-CMV-Cas9-V5-WPRE

    Plasmid

    #87904

    Purpose

    To produce lentiviral vector for gene editing

    Depositor

    Nicole Déglon

    Article

    Merienne et al Cell Rep. 2017 Sep 19;20(12):2980-2991.

    Insert

    Cas9-V5
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    human codon-optimized

    Promoter

    CMV

    Available since

    Sept. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDF1-MCS2-EF1-Puro-RGS7

    Plasmid

    #113728

    Purpose

    Express RGS7 in mamalian cells

    Depositor

    Yardena Samuels

    Article

    Qutob et al Sci Rep. 2018 Jan 12;8(1):653. doi: 10.1

    Insert

    RGS7 (RGS7 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. AOI-WT-Cas9-sg-mouse Suz12-F2-GFP

    Plasmid

    #91882

    Purpose

    Expresses 3xFLAG-Cas9 and a gRNA targeting mouse Suz12

    Depositor

    Martine Roussel

    Article

    Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

    Insert

    gRNA targeting Suz12 (Suz12 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 26, 2017

    Availability

    Academic Institutions and Nonprofits only

15. pαH-S-HRV3C/D614G

    Plasmid

    #164568

    Purpose

    SARS-CoV-2 Spike protein with furin site mutated to HRV3C site and D614G mutation (S-HRV3C-D614G variant)

    Depositor

    Priyamvada Acharya

    Article

    Gobeil et al Cell Rep. 2021 Jan 12;34(2):108630. doi:

    Insert

    Spike (S-HRV3C-D614G variant)
    

    Use

    Tags

    2X Strep-Tag II and 8X His tag

    Expression

    Mammalian

    Mutation

    D614G

    Promoter

    Available since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSPL3-ABCC8-27

    Plasmid

    #135919

    Purpose

    Encodes ABCC8 exon 27 wild type for the analysis of splicing variants

    Depositor

    Diana Valverde Pérez

    Article

    Lago-Docampo et al Sci Rep. 2020 Sep 15;10(1):15135. doi: 1

    Insert

    ATP-binding Cassette subfamily C member 8 (ABCC8 Human)
    

    Use

    Exon trapping

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    March 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLVX-CMV-XRCC1-gRNA res-Neo

    Plasmid

    #176149

    Purpose

    XRCC1 with dual PAM resistance to XRCC1 gRNA1 and XRCC1 gRNA2 & a neomycin/G418 resistance cassette

    Depositor

    Robert Sobol

    Article

    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

    Insert

    X-ray repair cross complementing 1 (XRCC1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Dual PAM resistance to XRCC1 gRNA 1 and XRCC1 gRN…

    Promoter

    CMV

    Available since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLV-CMV-EGFP-PolB-Hygro

    Plasmid

    #176056

    Purpose

    EGFP fused to the N-terminus of PolB & a hygromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

    Insert

    PolB (POLB Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PB-PE NG

    Plasmid

    #174554

    Purpose

    All-in-one prime editor piggyBac transposon, NG variant

    Depositor

    Tobias Cantz

    Article

    Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1

    Insert

    SpCas9 NG_H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
    

    Use

    CRISPR and Synthetic Biology; Piggybac transposon

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    L1111R, G1218R, E1219F, A1322R, R1335V, T1337R

    Promoter

    CAG

    Available since

    Feb. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Anti-TrpV3 [N15/39R]

    Plasmid

    #188210

    Purpose

    Mammalian Expression Plasmid of anti-TrpV3 (Rat). Derived from hybridoma N15/39

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    anti-TrpV3 (Rattus norvegicus) recombinant mouse monoclonal antibody (Trpv3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Dual CMV

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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