We narrowed to 68,416 results for: TOR;

Showing: 10801 - 10840 of 68416 results

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pShuttle.CC9

Plasmid

#192931

Purpose

Gateway compatible vector with BsaI cloning site and Cre-2A-Cas9 expression

Depositor

Thorsten Stiewe

Article

Merle et al Mol Cancer. 2022 Oct 3;21(1):191. doi: 1

Type

Empty backbone

Use

Gateway-compatible cloning vector

Promoter

CMV

Available Since

Dec. 9, 2022

Availability

Academic Institutions and Nonprofits only

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attB53_SNCB-_attB53

Plasmid

#183615

Purpose

RMCE vector to shuttle puromycin resistance (+), anti-GFP SynNotch receptor (+), tagBFP (-) and TRE-mCherry (-) cassettes into attP50-flanked landing pad (Addgene 183609). (+) = sense (-) = antisense.

Depositor

Sally Lowell

Article

Malaguti et al Development. 2022 May 26. pii: 275525. d

Inserts

LaG17 GFP nanobody SynNotch tTA
TRE-mCherry-SV40pA
tagBFP

Use

Rmce

Tags

Myc and human CD8a signal sequence

Expression

Mammalian

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pTS2308-Tier1-pGL3-PPGK-NLuc_TB

Plasmid

#169551

Purpose

Tier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' insulator sequence derived from the pGL3 plasmid (pGL3-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

pGL3-insulator-modulated PPGK-driven NanoLuc Luciferase - mTagBFP expression cassette

Expression

Mammalian

Promoter

PPGK

Available Since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pHR-PD-1 (BTLA ITIM)-mGFP

Plasmid

#180817

Purpose

Expression of human PD-1 with immunoreceptor tyrosine-based inhibitory motif (ITIM) from BTLA, fused to mEGFP

Depositor

Enfu Hui

Article

Xu et al Elife. 2021 Nov 4;10. pii: 74276. doi: 1

Insert

PD-1 (BTLA ITIM) (PDCD1 Human)

Use

Lentiviral

Tags

mEGFP

Mutation

replaced PD-1-ITIM (VDYGEL) with BTLA-ITIM (IVYAS…

Promoter

SFFV

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pET24a His6 GST PYL2(14-188)

Plasmid

#177842

Purpose

Bacterial Expression of PYL2

Depositor

Karsten Melcher

Article

Melcher et al Nature. 2009 Dec 3;462(7273):602-8. doi:

Insert

PYR1-like 2 (PYL2 Mustard Weed)

Tags

6xHis-GST

Expression

Bacterial

Available Since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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XLone-Puro Cas13d-eGFP U6 RUNX1 g1

Plasmid

#155185

Purpose

PiggybacTransposon-based tunable and temporal expression control of Cas13d- eGFP and RUNX1 gRNA1

Depositor

Xiaoping Bao

Article

Chang et al Biomaterials. 2022 May 6;285:121569. doi

Insert

Cas13d RUNX1 gRNA1

Use

Piggybac transposon

Expression

Mammalian

Promoter

U6

Available Since

June 24, 2021

Availability

Academic Institutions and Nonprofits only

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KIF13B

Plasmid

#163907

Purpose

Full length mouse KIF13B for purification from insect cells

Depositor

Martin Loose

Article

Duellberg et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

Insert

kif13b (Kif13b Mouse)

Tags

Cleavable 6 x HIS tag

Expression

Insect

Promoter

polyhedrin

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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KIF13B 1-584

Plasmid

#163908

Purpose

Motile fragment of mouse KIF13B (aa 1-584) for purification from insect cells.

Depositor

Martin Loose

Article

Duellberg et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

Insert

kif13b (Kif13b Mouse)

Tags

Cleavable 6 x HIS tag

Expression

Insect

Promoter

polyhedrin

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pPICZ-aC-VTS-US

Plasmid

#160425

Purpose

Expression of c-luciferase from sp. Vargula tsujii (USA)

Depositor

Todd Oakley

Article

Hensley et al Mol Ecol. 2020 Oct 8. doi: 10.1111/mec.1

Insert

luciferase

Use

Luciferase

Tags

6x Histidine tag, Yeast alpha secretion factor, c…

Expression

Yeast

Mutation

See depositor comments below

Promoter

AOX

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pPICZ-aC-MSVU-BZ

Plasmid

#160426

Purpose

Expression of c-luciferase from sp. Maristella sp. SVU (Belize)

Depositor

Todd Oakley

Article

Hensley et al Mol Ecol. 2020 Oct 8. doi: 10.1111/mec.1

Insert

luciferase

Use

Luciferase

Tags

6x Histidine tag, Yeast alpha secretion factor, c…

Expression

Yeast

Mutation

See depositor comments below

Promoter

AOX

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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BtGRK2[D110A K220R]-mVenus

Plasmid

#137779

Purpose

Visualization of GRK2

Depositor

Dorus Gadella

Article

Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

Insert

Bovine GRK2[D110A K220R] (GRK2 Bovine)

Expression

Mammalian

Mutation

D110A K220R

Promoter

CMV

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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HsGRK6[L66A E514A]-sYFP2

Plasmid

#137781

Purpose

Visualization of GRK6

Depositor

Dorus Gadella

Article

Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

Insert

Human GRK6[L66A E514A] (GRK6 Human)

Expression

Mammalian

Mutation

L66A E514A

Promoter

CMV

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pMS22

Plasmid

#129710

Purpose

Expresses Escherichia coli RssB E134G:E135S:E136G:E137S variant as an N-terminal 10His fusion

Depositor

Alexandra Deaconescu

Article

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

Escherichia coli RssB-GSGS variant (rssB Escherichia coli MG1655)

Tags

10-Histidine tag cleavable with PreScission Prote…

Expression

Bacterial

Mutation

RssB E134G:E135S:E136G:E137S

Promoter

T7

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pFB-U6-Chrnb4-gRNA-hSyn-mCherry-WPRE-SV40pA

Plasmid

#128345

Purpose

pAAV encoding gRNA sequence for loss-of-function indels

Depositor

Ryan Drenan

Article

Peng et al Eur J Neurosci. 2018 May 19. doi: 10.111

Insert

Chrnb4 (Chrnb4 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

July 19, 2019

Availability

Academic Institutions and Nonprofits only

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Opto-GPR152

Plasmid

#106052

Purpose

Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR152; Opto-GPR152; E6)

Depositor

Harald Janovjak

Article

Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

Insert

Opto-GPR152 (GPR152 Bovine, Human)

Tags

Rhodopsin-1D4 and VSV-G

Expression

Mammalian

Mutation

Chimeric receptor protein

Promoter

CMV

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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Opto-GPR176

Plasmid

#106060

Purpose

Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR176; Opto-GPR176; F2)

Depositor

Harald Janovjak

Article

Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

Insert

Opto-GPR176 (GPR176 Bovine, Human)

Tags

Rhodopsin-1D4 and VSV-G

Expression

Mammalian

Mutation

Chimeric receptor protein

Promoter

CMV

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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Opto-GPR183

Plasmid

#106062

Purpose

Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR183; Opto-GPR183; F4)

Depositor

Harald Janovjak

Article

Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

Insert

Opto-GPR183 (GPR183 Bovine, Human)

Tags

Rhodopsin-1D4 and VSV-G

Expression

Mammalian

Mutation

Chimeric receptor protein

Promoter

CMV

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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Opto-GPR132

Plasmid

#106042

Purpose

Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR132; Opto-GPR132; D8)

Depositor

Harald Janovjak

Article

Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

Insert

Opto-GPR132 (GPR132 Bovine, Human)

Tags

Rhodopsin-1D4 and VSV-G

Expression

Mammalian

Mutation

Chimeric receptor protein

Promoter

CMV

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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Opto-GPR141

Plasmid

#106045

Purpose

Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR141; Opto-GPR141; D11)

Depositor

Harald Janovjak

Article

Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

Insert

Opto-GPR141 (GPR141 Bovine, Human)

Tags

Rhodopsin-1D4 and VSV-G

Expression

Mammalian

Mutation

Chimeric receptor protein

Promoter

CMV

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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Opto-GPR35

Plasmid

#106018

Purpose

Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR35; Opto-GPR35; B8)

Depositor

Harald Janovjak

Article

Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

Insert

Opto-GPR35 (GPR35 Bovine, Human)

Tags

Rhodopsin-1D4 and VSV-G

Expression

Mammalian

Mutation

Chimeric receptor protein

Promoter

CMV

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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Opto-GPR50

Plasmid

#106024

Purpose

Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR50; Opto-GPR50; C2)

Depositor

Harald Janovjak

Article

Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

Insert

Opto-GPR50 (GPR50 Bovine, Human)

Tags

Rhodopsin-1D4 and VSV-G

Expression

Mammalian

Mutation

Chimeric receptor protein

Promoter

CMV

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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Opto-GPR62

Plasmid

#106028

Purpose

Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR62; Opto-GPR62; C6)

Depositor

Harald Janovjak

Article

Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

Insert

Opto-GPR62 (GPR62 Bovine, Human)

Tags

Rhodopsin-1D4 and VSV-G

Expression

Mammalian

Mutation

Chimeric receptor protein

Promoter

CMV

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Opto-GPR15

Plasmid

#106004

Purpose

Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR15; Opto-GPR15; A6)

Depositor

Harald Janovjak

Article

Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

Insert

Opto-GPR15 (GPR15 Bovine, Human)

Tags

Rhodopsin-1D4 and VSV-G

Expression

Mammalian

Mutation

Chimeric receptor protein

Promoter

CMV

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Opto-GPR33

Plasmid

#106016

Purpose

Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR33; Opto-GPR33; B6)

Depositor

Harald Janovjak

Article

Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

Insert

Opto-GPR33 (GPR33 Bovine, Human)

Tags

Rhodopsin-1D4 and VSV-G

Expression

Mammalian

Mutation

Chimeric receptor protein

Promoter

CMV

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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[pSK480] pLJC5 Flag-Depdc5(A)

Plasmid

#109338

Purpose

Lenti-viral expression of Flag-tagged Depdc5 mutant A

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Nature. 2018 Apr 5;556(7699):64-69. doi:

Insert

Depdc5 (DEPDC5 Human)

Use

Lentiviral

Tags

FLAG

Mutation

DIYGD(185-189)GSGSG

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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[pSK481] pLJC5 Flag-Depdc5(B)

Plasmid

#109339

Purpose

Lenti-viral expression of Flag-tagged Depdc5 mutant B

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Nature. 2018 Apr 5;556(7699):64-69. doi:

Insert

Depdc5 (DEPDC5 Human)

Use

Lentiviral

Tags

FLAG

Mutation

EQPLH(371-375)GSGSG

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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[pSK479] pLJC5 Flag-Depdc5(C)

Plasmid

#109340

Purpose

Lenti-viral expression of Flag-tagged Depdc5 mutant C

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Nature. 2018 Apr 5;556(7699):64-69. doi:

Insert

Depdc5 (DEPDC5 Human)

Use

Lentiviral

Tags

FLAG

Mutation

FTPRI(419-423)GSGSG

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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pSN31

Plasmid

#100116

Purpose

expresses a chimeric KLF4-activator fusion

Depositor

Mark Ptashne

Article

Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

Insert

mCherry-P2A-KLF4 fused to a 'medium' VP16 domain (KLF4 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pSN32

Plasmid

#100117

Purpose

expresses a chimeric KLF4-activator fusion

Depositor

Mark Ptashne

Article

Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

Insert

mCherry-P2A-KLF4 fused to a 'weak' VP16 domain (KLF4 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pLPCX2-HA-GATA3 N1(1-259)

Plasmid

#89830

Purpose

Express GATA3 N1 in mammalian cells

Depositor

Shengyu Yang

Article

Sun et al J Biol Chem. 2013 Dec 27;288(52):36971-8

Insert

Gata3 (Gata3 Mouse)

Use

Retroviral

Tags

HA

Mutation

N1 (AA 1-259)

Promoter

CMV

Available Since

May 11, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pLPCX2-HA-GATA3 C1(295-443)

Plasmid

#90316

Purpose

Express GATA3 C2 in mammalian cells

Depositor

Shengyu Yang

Article

Sun et al J Biol Chem. 2013 Dec 27;288(52):36971-8

Insert

GATA3 (Gata3 Mouse)

Use

Retroviral

Tags

HA

Mutation

C2 (AA 295-443)

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pLPCX2-HA-GATA3 N2(1-294)

Plasmid

#89831

Purpose

Express GATA3 N2 in mammalian cells

Depositor

Shengyu Yang

Article

Sun et al J Biol Chem. 2013 Dec 27;288(52):36971-8

Insert

Gata3 (Gata3 Mouse)

Use

Retroviral

Tags

HA

Mutation

N2 (AA 1-294)

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
C0062 (luxR)_CD

Plasmid

#66028

Purpose

MoClo Basic Part: CDS - Controller protein, luxR repressor/activator (in concert with HSL, represses pLuxR(pR) R0063. Also up-regulates pLuxR(pL) R0062) [C:C0062:D]

Depositor

Douglas Densmore

Article

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Insert

Transcription factor

Use

Synthetic Biology

Available Since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pUT1147-HQ858691

Plasmid

#27737

Depositor

John Gray, Erich Grotewold

Article

Burdo et al Plant J. 2014 Oct;80(2):356-66. doi: 10.

Insert

ZmMYB2 transcription factor from AM156904 (myb2 Zea mays)

Use

Entry vector

Available Since

March 25, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pUT1148-HQ858692

Plasmid

#27738

Depositor

John Gray, Erich Grotewold

Article

Burdo et al Plant J. 2014 Oct;80(2):356-66. doi: 10.

Insert

ZmMYB8 transcription factor from AM156905 (myb8 Zea mays)

Use

Entry vector

Available Since

March 25, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pUT1149-HQ858693

Plasmid

#27739

Depositor

John Gray, Erich Grotewold

Article

Burdo et al Plant J. 2014 Oct;80(2):356-66. doi: 10.

Insert

ZmMYB39 transcription factor from AM156907 (myb39 Zea mays)

Use

Entry vector

Available Since

March 25, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pUT1150-HQ858694

Plasmid

#27740

Depositor

John Gray, Erich Grotewold

Article

Burdo et al Plant J. 2014 Oct;80(2):356-66. doi: 10.

Insert

ZmMYB42 transcription factor from AM156908 (myb42 Zea mays)

Use

Entry vector

Available Since

March 25, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pEN_hU6miR-Gb2-K

Plasmid

#25759

Purpose

Entry vector with human U6 promoter driving both mouse G alpha 12 and G alpha 13 miR30-based shRNAs.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Insert

Gb2 miR-shRNA (Gnb2 Mouse)

Use

Entry vector

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pCin4-3xFLAG-MKL1 L555A

Plasmid

#19851

Depositor

Ron Prywes

Article

Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

Insert

MKL1 L555A (MRTFA Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

changed Leucine 555 to Alanine

Available Since

Feb. 12, 2009

Availability

Academic Institutions and Nonprofits only
pET28a-MED20

Plasmid

#15435

Depositor

Joan Conaway, Ronald Conaway

Article

Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

Insert

MED20 (Med20 Mouse)

Tags

His

Expression

Bacterial

Mutation

N/A

Available Since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only

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We narrowed to 68,416 results for: TOR;